Candidatus Ishikawaella capsulata Mpkobe

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Candidatus Ishikawaella; Candidatus Ishikawaella capsulata

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 623 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5WDN2|C5WDN2_9ENTR ATP-dependent protease subunit HslV OS=Candidatus Ishikawaella capsulata Mpkobe OX=476281 GN=hslV PE=3 SV=1
MM1 pKa = 7.41INVVLLEE8 pKa = 3.86VMMIFTKK15 pKa = 10.49FAIGQQVRR23 pKa = 11.84HH24 pKa = 5.99KK25 pKa = 10.07MSGLLGVIIDD35 pKa = 3.83VDD37 pKa = 3.82PEE39 pKa = 4.22YY40 pKa = 11.49SLTTPKK46 pKa = 10.69LEE48 pKa = 3.86EE49 pKa = 4.35FGVSEE54 pKa = 5.16SLRR57 pKa = 11.84TDD59 pKa = 3.06PWYY62 pKa = 10.65HH63 pKa = 6.45VIIEE67 pKa = 4.4NYY69 pKa = 9.88SGEE72 pKa = 4.03AVYY75 pKa = 9.25TYY77 pKa = 10.57VAEE80 pKa = 4.1VQLYY84 pKa = 10.37GEE86 pKa = 4.64VLCEE90 pKa = 4.57HH91 pKa = 7.43LDD93 pKa = 3.75QPSLDD98 pKa = 3.94EE99 pKa = 4.38LASSIRR105 pKa = 11.84QQLQEE110 pKa = 3.87PCLRR114 pKa = 11.84HH115 pKa = 5.53

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5WC40|C5WC40_9ENTR Superoxide dismutase OS=Candidatus Ishikawaella capsulata Mpkobe OX=476281 GN=sodA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLIKK11 pKa = 10.42RR12 pKa = 11.84NRR14 pKa = 11.84AHH16 pKa = 7.6GFRR19 pKa = 11.84ARR21 pKa = 11.84MASKK25 pKa = 10.41NGRR28 pKa = 11.84QILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.65GRR39 pKa = 11.84MRR41 pKa = 11.84LTISDD46 pKa = 3.42RR47 pKa = 11.84KK48 pKa = 9.39YY49 pKa = 10.75

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

623

0

623

204056

30

1415

327.5

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.953 ± 0.082

1.42 ± 0.037

4.534 ± 0.056

5.374 ± 0.079

4.062 ± 0.068

6.017 ± 0.081

2.426 ± 0.039

10.793 ± 0.099

7.339 ± 0.081

10.17 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.04

6.386 ± 0.07

3.513 ± 0.042

3.648 ± 0.047

4.507 ± 0.064

6.27 ± 0.061

4.957 ± 0.055

5.391 ± 0.074

1.016 ± 0.036

3.685 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski