Beet chlorosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91AV8|Q91AV8_9LUTE Movement protein OS=Beet chlorosis virus OX=131082 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.23AQSTDD6 pKa = 3.76DD7 pKa = 5.06AISLYY12 pKa = 11.07DD13 pKa = 3.49MPSQRR18 pKa = 11.84FRR20 pKa = 11.84YY21 pKa = 9.81IEE23 pKa = 4.39DD24 pKa = 3.74EE25 pKa = 4.12NMNWTNLDD33 pKa = 3.7SRR35 pKa = 11.84WYY37 pKa = 9.68SQNSLKK43 pKa = 10.38AIPMVIVPVPQGEE56 pKa = 4.3WTVEE60 pKa = 3.43ISMEE64 pKa = 4.75GYY66 pKa = 10.13QPTSSTTDD74 pKa = 3.57PNKK77 pKa = 10.65DD78 pKa = 3.46KK79 pKa = 11.14QDD81 pKa = 3.38GLIAYY86 pKa = 10.04NDD88 pKa = 3.83DD89 pKa = 5.26LKK91 pKa = 11.19EE92 pKa = 3.95GWNVGVYY99 pKa = 10.95NNVEE103 pKa = 3.8ITNNKK108 pKa = 9.19ADD110 pKa = 3.54NTLKK114 pKa = 10.77YY115 pKa = 9.84GHH117 pKa = 7.74PDD119 pKa = 3.01MEE121 pKa = 5.18LNSCHH126 pKa = 7.01FNQQQCLEE134 pKa = 4.15RR135 pKa = 11.84DD136 pKa = 3.51GDD138 pKa = 4.14LTCHH142 pKa = 6.89IKK144 pKa = 8.06TTGDD148 pKa = 3.14NASFFIVGPAVQKK161 pKa = 9.91QSKK164 pKa = 8.52YY165 pKa = 10.45NYY167 pKa = 8.79AVSYY171 pKa = 8.9GAWTDD176 pKa = 3.24RR177 pKa = 11.84MVEE180 pKa = 3.54IGMIAIALDD189 pKa = 3.86EE190 pKa = 4.27QGSSGSARR198 pKa = 11.84TEE200 pKa = 3.59RR201 pKa = 11.84PKK203 pKa = 10.79RR204 pKa = 11.84VGHH207 pKa = 5.9SMAVSTWEE215 pKa = 4.23TINLPEE221 pKa = 6.06KK222 pKa = 10.51EE223 pKa = 4.52DD224 pKa = 3.8SEE226 pKa = 4.47KK227 pKa = 11.19LKK229 pKa = 10.17TGQRR233 pKa = 11.84QDD235 pKa = 3.15LKK237 pKa = 10.52TPFTISGSSDD247 pKa = 2.96VKK249 pKa = 11.02GIEE252 pKa = 4.58KK253 pKa = 10.08RR254 pKa = 11.84DD255 pKa = 3.61LPLPADD261 pKa = 3.75EE262 pKa = 6.24DD263 pKa = 3.75IPDD266 pKa = 5.43FIGNDD271 pKa = 3.0PWSNVSIRR279 pKa = 11.84KK280 pKa = 8.49LQEE283 pKa = 3.6EE284 pKa = 4.31EE285 pKa = 4.52AMTSKK290 pKa = 10.69SGLRR294 pKa = 11.84PQLKK298 pKa = 10.07PPGLPKK304 pKa = 9.66PQPVRR309 pKa = 11.84TIGNFNPTPEE319 pKa = 4.75LVEE322 pKa = 3.88SWRR325 pKa = 11.84PDD327 pKa = 3.39VNPGYY332 pKa = 10.48SKK334 pKa = 11.14EE335 pKa = 4.09DD336 pKa = 3.27VAAATILYY344 pKa = 9.67GGSIKK349 pKa = 10.53DD350 pKa = 3.63GRR352 pKa = 11.84SMIDD356 pKa = 3.06KK357 pKa = 10.07RR358 pKa = 11.84DD359 pKa = 3.47KK360 pKa = 11.16AVLDD364 pKa = 4.22GRR366 pKa = 11.84KK367 pKa = 8.85HH368 pKa = 5.5WGSSLASSLTGGTLKK383 pKa = 10.92ASAKK387 pKa = 9.49SEE389 pKa = 3.99KK390 pKa = 9.94LAKK393 pKa = 9.1LTSRR397 pKa = 11.84EE398 pKa = 3.62RR399 pKa = 11.84AEE401 pKa = 3.8FEE403 pKa = 4.32RR404 pKa = 11.84IKK406 pKa = 10.41RR407 pKa = 11.84QQGTTQASEE416 pKa = 3.97YY417 pKa = 11.04LEE419 pKa = 5.44FILKK423 pKa = 10.35SMNPDD428 pKa = 2.86

Molecular weight: 47.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q91AW0|Q91AW0_9LUTE Serine protease OS=Beet chlorosis virus OX=131082 PE=4 SV=1
MM1 pKa = 6.49NTVVGRR7 pKa = 11.84RR8 pKa = 11.84TINGRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PRR17 pKa = 11.84RR18 pKa = 11.84QTRR21 pKa = 11.84RR22 pKa = 11.84AQRR25 pKa = 11.84NQPVVVVQTSRR36 pKa = 11.84RR37 pKa = 11.84TQRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GNNRR51 pKa = 11.84AGRR54 pKa = 11.84TVSTRR59 pKa = 11.84GTGQSEE65 pKa = 4.45TFVFSEE71 pKa = 4.69DD72 pKa = 3.37NLAGSSSGAITFGPSLSDD90 pKa = 4.1CPAFSNGMLKK100 pKa = 10.2AYY102 pKa = 10.09HH103 pKa = 6.71EE104 pKa = 4.71YY105 pKa = 9.92KK106 pKa = 10.07ISMVILEE113 pKa = 4.63FVSEE117 pKa = 4.33ASSQSSGSIAYY128 pKa = 9.47EE129 pKa = 3.85LDD131 pKa = 3.29PHH133 pKa = 6.98CKK135 pKa = 10.11LNSLSSTINKK145 pKa = 9.78FGITKK150 pKa = 9.13PGRR153 pKa = 11.84RR154 pKa = 11.84AFTASYY160 pKa = 10.78INGTEE165 pKa = 3.62WHH167 pKa = 7.18DD168 pKa = 3.61VAKK171 pKa = 10.54DD172 pKa = 3.35QFRR175 pKa = 11.84ILYY178 pKa = 8.84KK179 pKa = 11.0GNGSSSIAGSFRR191 pKa = 11.84ITIKK195 pKa = 10.75CHH197 pKa = 4.21FHH199 pKa = 6.02NPKK202 pKa = 10.5

Molecular weight: 22.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2133

175

1080

426.6

47.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.391 ± 0.662

1.641 ± 0.353

5.204 ± 0.766

6.142 ± 0.474

3.516 ± 0.459

6.892 ± 0.521

2.532 ± 0.638

5.11 ± 0.508

5.767 ± 0.682

9.048 ± 1.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.28

4.594 ± 0.441

5.11 ± 0.47

3.985 ± 0.326

6.423 ± 1.244

9.47 ± 0.985

6.517 ± 0.591

5.579 ± 0.825

1.969 ± 0.387

2.813 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski