Helicobacter phage Pt1293U
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S5RGS6|A0A1S5RGS6_9CAUD Structural protein OS=Helicobacter phage Pt1293U OX=1852669 PE=4 SV=1
MM1 pKa = 7.66 GIKK4 pKa = 9.82 EE5 pKa = 4.23 KK6 pKa = 10.55 EE7 pKa = 3.99 IEE9 pKa = 4.18 LEE11 pKa = 3.99 TLKK14 pKa = 11.01 RR15 pKa = 11.84 EE16 pKa = 3.99 IAQAEE21 pKa = 4.06 ASLEE25 pKa = 3.89 QDD27 pKa = 4.59 FIKK30 pKa = 10.97 HH31 pKa = 5.29 MVDD34 pKa = 2.81 KK35 pKa = 10.6 TNEE38 pKa = 3.95 KK39 pKa = 10.6 VEE41 pKa = 4.81 DD42 pKa = 3.83 LFFSNKK48 pKa = 8.85 PEE50 pKa = 4.27 FYY52 pKa = 10.1 RR53 pKa = 11.84 FVFTEE58 pKa = 3.6 QNNYY62 pKa = 9.57 LRR64 pKa = 11.84 EE65 pKa = 4.0 KK66 pKa = 10.13 LTDD69 pKa = 3.41 KK70 pKa = 10.83 VGRR73 pKa = 11.84 AMDD76 pKa = 4.45 LSDD79 pKa = 4.84 EE80 pKa = 4.16 IQRR83 pKa = 11.84 DD84 pKa = 3.19 KK85 pKa = 11.29 DD86 pKa = 3.41 AEE88 pKa = 4.19 EE89 pKa = 4.01 IEE91 pKa = 4.13 KK92 pKa = 11.03 DD93 pKa = 3.3 KK94 pKa = 11.44 EE95 pKa = 4.14 AFLKK99 pKa = 10.04 KK100 pKa = 10.16 HH101 pKa = 6.21 PGIDD105 pKa = 3.74 FNEE108 pKa = 3.89 LLEE111 pKa = 4.62 FYY113 pKa = 10.85 NEE115 pKa = 3.93 EE116 pKa = 3.79 VPNRR120 pKa = 11.84 FKK122 pKa = 11.17 KK123 pKa = 10.62 QIDD126 pKa = 3.63 KK127 pKa = 11.4 LEE129 pKa = 4.21 GVAFFEE135 pKa = 5.75 AILDD139 pKa = 3.82 YY140 pKa = 11.38 FEE142 pKa = 4.71 VLNSKK147 pKa = 10.43 PEE149 pKa = 3.98 EE150 pKa = 4.2 GQKK153 pKa = 10.54 EE154 pKa = 4.18 EE155 pKa = 4.64 EE156 pKa = 4.49 KK157 pKa = 11.24 NNLPKK162 pKa = 10.47 EE163 pKa = 4.11 VLGNGVSGVGYY174 pKa = 10.77 ANNEE178 pKa = 4.31 NIMTRR183 pKa = 11.84 YY184 pKa = 9.53
Molecular weight: 21.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.82
IPC2_protein 4.736
IPC_protein 4.647
Toseland 4.507
ProMoST 4.724
Dawson 4.571
Bjellqvist 4.711
Wikipedia 4.393
Rodwell 4.482
Grimsley 4.418
Solomon 4.558
Lehninger 4.52
Nozaki 4.673
DTASelect 4.762
Thurlkill 4.495
EMBOSS 4.418
Sillero 4.749
Patrickios 4.24
IPC_peptide 4.571
IPC2_peptide 4.749
IPC2.peptide.svr19 4.695
Protein with the highest isoelectric point:
>tr|A0A1S5RGR5|A0A1S5RGR5_9CAUD Portal protein OS=Helicobacter phage Pt1293U OX=1852669 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.32 NINLIKK8 pKa = 10.35 HH9 pKa = 5.56 FRR11 pKa = 11.84 NVEE14 pKa = 4.15 NIKK17 pKa = 10.26 KK18 pKa = 9.84 RR19 pKa = 11.84 HH20 pKa = 5.11 LAFKK24 pKa = 10.13 KK25 pKa = 9.42 SKK27 pKa = 10.39 KK28 pKa = 9.7 RR29 pKa = 11.84 VLEE32 pKa = 3.84 FLKK35 pKa = 10.89 NRR37 pKa = 11.84 GYY39 pKa = 11.0 RR40 pKa = 11.84 DD41 pKa = 4.54 FIAKK45 pKa = 9.48 VKK47 pKa = 10.65 SKK49 pKa = 10.48 NQSDD53 pKa = 4.27 DD54 pKa = 4.31 EE55 pKa = 4.1 ILEE58 pKa = 4.09 NLEE61 pKa = 4.16 LSYY64 pKa = 11.47 LKK66 pKa = 10.42 II67 pKa = 4.09
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 9.487
IPC_protein 9.443
Toseland 10.526
ProMoST 9.984
Dawson 10.599
Bjellqvist 10.175
Wikipedia 10.701
Rodwell 11.316
Grimsley 10.628
Solomon 10.628
Lehninger 10.628
Nozaki 10.482
DTASelect 10.175
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.511
Patrickios 11.067
IPC_peptide 10.643
IPC2_peptide 8.492
IPC2.peptide.svr19 8.592
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
0
36
9542
43
880
265.1
30.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.487 ± 0.318
0.796 ± 0.142
5.02 ± 0.429
8.457 ± 0.423
4.758 ± 0.245
4.024 ± 0.238
1.572 ± 0.178
6.676 ± 0.247
11.109 ± 0.422
10.836 ± 0.431
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.897 ± 0.179
8.195 ± 0.434
2.389 ± 0.17
4.601 ± 0.318
3.836 ± 0.19
6.445 ± 0.213
4.674 ± 0.285
4.244 ± 0.337
0.472 ± 0.083
3.511 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here