Amino acid dipepetide frequency for Helicobacter phage Pt1293U

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.258AlaAla: 1.258 ± 0.41
0.629AlaCys: 0.629 ± 0.29
1.887AlaAsp: 1.887 ± 0.327
3.564AlaGlu: 3.564 ± 0.756
3.773AlaPhe: 3.773 ± 0.725
2.62AlaGly: 2.62 ± 0.682
0.629AlaHis: 0.629 ± 0.228
4.507AlaIle: 4.507 ± 0.713
8.804AlaLys: 8.804 ± 0.959
10.691AlaLeu: 10.691 ± 1.211
1.782AlaMet: 1.782 ± 0.485
6.708AlaAsn: 6.708 ± 1.043
1.258AlaPro: 1.258 ± 0.28
2.411AlaGln: 2.411 ± 0.525
2.515AlaArg: 2.515 ± 0.515
3.773AlaSer: 3.773 ± 0.558
3.249AlaThr: 3.249 ± 0.603
1.887AlaVal: 1.887 ± 0.481
0.314AlaTrp: 0.314 ± 0.205
2.62AlaTyr: 2.62 ± 0.525
0.0AlaXaa: 0.0 ± 0.0
Cys
0.943CysAla: 0.943 ± 0.291
0.0CysCys: 0.0 ± 0.0
0.629CysAsp: 0.629 ± 0.384
0.734CysGlu: 0.734 ± 0.265
0.629CysPhe: 0.629 ± 0.264
0.734CysGly: 0.734 ± 0.354
0.0CysHis: 0.0 ± 0.0
0.629CysIle: 0.629 ± 0.34
0.21CysLys: 0.21 ± 0.156
1.258CysLeu: 1.258 ± 0.409
0.0CysMet: 0.0 ± 0.0
0.314CysAsn: 0.314 ± 0.164
0.314CysPro: 0.314 ± 0.169
0.314CysGln: 0.314 ± 0.177
0.105CysArg: 0.105 ± 0.098
0.105CysSer: 0.105 ± 0.109
0.419CysThr: 0.419 ± 0.208
0.419CysVal: 0.419 ± 0.221
0.0CysTrp: 0.0 ± 0.0
0.21CysTyr: 0.21 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
2.411AspAla: 2.411 ± 0.401
0.314AspCys: 0.314 ± 0.233
1.572AspAsp: 1.572 ± 0.378
4.297AspGlu: 4.297 ± 0.771
4.192AspPhe: 4.192 ± 0.552
1.048AspGly: 1.048 ± 0.301
0.419AspHis: 0.419 ± 0.166
2.83AspIle: 2.83 ± 0.802
6.079AspLys: 6.079 ± 1.065
8.07AspLeu: 8.07 ± 0.979
1.258AspMet: 1.258 ± 0.543
4.088AspAsn: 4.088 ± 0.657
1.258AspPro: 1.258 ± 0.356
1.363AspGln: 1.363 ± 0.38
2.306AspArg: 2.306 ± 0.52
2.201AspSer: 2.201 ± 0.433
1.991AspThr: 1.991 ± 0.592
1.048AspVal: 1.048 ± 0.335
0.21AspTrp: 0.21 ± 0.141
3.249AspTyr: 3.249 ± 0.619
0.0AspXaa: 0.0 ± 0.0
Glu
6.289GluAla: 6.289 ± 0.683
0.419GluCys: 0.419 ± 0.195
2.306GluAsp: 2.306 ± 0.495
4.612GluGlu: 4.612 ± 0.881
4.088GluPhe: 4.088 ± 0.615
1.048GluGly: 1.048 ± 0.321
1.258GluHis: 1.258 ± 0.366
7.337GluIle: 7.337 ± 0.825
8.28GluLys: 8.28 ± 0.971
9.852GluLeu: 9.852 ± 1.412
1.363GluMet: 1.363 ± 0.356
6.813GluAsn: 6.813 ± 0.708
1.467GluPro: 1.467 ± 0.434
5.241GluGln: 5.241 ± 1.048
4.926GluArg: 4.926 ± 0.709
7.546GluSer: 7.546 ± 0.999
4.297GluThr: 4.297 ± 0.673
4.821GluVal: 4.821 ± 0.862
0.419GluTrp: 0.419 ± 0.207
2.201GluTyr: 2.201 ± 0.424
0.0GluXaa: 0.0 ± 0.0
Phe
1.363PheAla: 1.363 ± 0.431
0.734PheCys: 0.734 ± 0.338
2.725PheAsp: 2.725 ± 0.58
3.564PheGlu: 3.564 ± 0.543
2.935PhePhe: 2.935 ± 0.688
1.363PheGly: 1.363 ± 0.268
0.838PheHis: 0.838 ± 0.208
2.725PheIle: 2.725 ± 0.425
6.498PheLys: 6.498 ± 0.706
6.184PheLeu: 6.184 ± 0.886
0.419PheMet: 0.419 ± 0.286
4.088PheAsn: 4.088 ± 0.574
0.21PhePro: 0.21 ± 0.117
0.943PheGln: 0.943 ± 0.361
2.306PheArg: 2.306 ± 0.471
4.297PheSer: 4.297 ± 0.628
2.411PheThr: 2.411 ± 0.513
1.782PheVal: 1.782 ± 0.35
0.21PheTrp: 0.21 ± 0.12
1.991PheTyr: 1.991 ± 0.579
0.0PheXaa: 0.0 ± 0.0
Gly
2.935GlyAla: 2.935 ± 0.995
0.524GlyCys: 0.524 ± 0.212
1.887GlyAsp: 1.887 ± 0.469
2.515GlyGlu: 2.515 ± 0.435
2.725GlyPhe: 2.725 ± 0.513
3.04GlyGly: 3.04 ± 0.61
0.419GlyHis: 0.419 ± 0.204
3.249GlyIle: 3.249 ± 0.636
2.096GlyLys: 2.096 ± 0.369
4.297GlyLeu: 4.297 ± 0.57
1.467GlyMet: 1.467 ± 0.337
2.725GlyAsn: 2.725 ± 0.483
0.0GlyPro: 0.0 ± 0.0
1.153GlyGln: 1.153 ± 0.339
1.048GlyArg: 1.048 ± 0.282
3.668GlySer: 3.668 ± 0.61
1.153GlyThr: 1.153 ± 0.34
3.773GlyVal: 3.773 ± 0.769
0.0GlyTrp: 0.0 ± 0.0
1.572GlyTyr: 1.572 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
0.943HisAla: 0.943 ± 0.287
0.105HisCys: 0.105 ± 0.102
1.048HisAsp: 1.048 ± 0.449
1.467HisGlu: 1.467 ± 0.32
0.943HisPhe: 0.943 ± 0.288
0.314HisGly: 0.314 ± 0.171
0.105HisHis: 0.105 ± 0.119
0.838HisIle: 0.838 ± 0.33
1.782HisLys: 1.782 ± 0.422
1.782HisLeu: 1.782 ± 0.57
0.21HisMet: 0.21 ± 0.155
1.572HisAsn: 1.572 ± 0.474
0.524HisPro: 0.524 ± 0.229
0.524HisGln: 0.524 ± 0.301
0.419HisArg: 0.419 ± 0.175
1.153HisSer: 1.153 ± 0.325
0.838HisThr: 0.838 ± 0.259
0.105HisVal: 0.105 ± 0.105
0.0HisTrp: 0.0 ± 0.0
1.048HisTyr: 1.048 ± 0.361
0.0HisXaa: 0.0 ± 0.0
Ile
4.821IleAla: 4.821 ± 0.6
0.943IleCys: 0.943 ± 0.419
3.983IleAsp: 3.983 ± 0.583
5.136IleGlu: 5.136 ± 0.74
1.572IlePhe: 1.572 ± 0.447
2.306IleGly: 2.306 ± 0.506
1.048IleHis: 1.048 ± 0.375
3.354IleIle: 3.354 ± 0.488
8.28IleLys: 8.28 ± 1.17
6.708IleLeu: 6.708 ± 0.578
0.734IleMet: 0.734 ± 0.215
4.716IleAsn: 4.716 ± 0.628
1.258IlePro: 1.258 ± 0.387
4.192IleGln: 4.192 ± 0.564
3.04IleArg: 3.04 ± 0.539
4.088IleSer: 4.088 ± 0.515
4.192IleThr: 4.192 ± 0.663
3.249IleVal: 3.249 ± 0.63
0.21IleTrp: 0.21 ± 0.146
2.935IleTyr: 2.935 ± 0.536
0.0IleXaa: 0.0 ± 0.0
Lys
8.804LysAla: 8.804 ± 0.985
0.419LysCys: 0.419 ± 0.222
7.442LysAsp: 7.442 ± 1.038
12.997LysGlu: 12.997 ± 1.355
3.04LysPhe: 3.04 ± 0.354
3.354LysGly: 3.354 ± 0.577
3.354LysHis: 3.354 ± 0.662
8.49LysIle: 8.49 ± 0.974
9.957LysLys: 9.957 ± 1.381
8.385LysLeu: 8.385 ± 0.973
1.467LysMet: 1.467 ± 0.453
10.271LysAsn: 10.271 ± 1.227
3.983LysPro: 3.983 ± 0.584
5.241LysGln: 5.241 ± 0.76
5.45LysArg: 5.45 ± 0.783
5.031LysSer: 5.031 ± 0.796
5.765LysThr: 5.765 ± 0.881
4.402LysVal: 4.402 ± 0.698
0.629LysTrp: 0.629 ± 0.256
2.62LysTyr: 2.62 ± 0.507
0.0LysXaa: 0.0 ± 0.0
Leu
6.813LeuAla: 6.813 ± 0.985
1.572LeuCys: 1.572 ± 0.556
5.345LeuAsp: 5.345 ± 0.623
11.32LeuGlu: 11.32 ± 1.376
3.144LeuPhe: 3.144 ± 0.664
5.974LeuGly: 5.974 ± 0.737
0.734LeuHis: 0.734 ± 0.325
6.079LeuIle: 6.079 ± 0.711
17.923LeuLys: 17.923 ± 1.52
8.07LeuLeu: 8.07 ± 0.998
2.096LeuMet: 2.096 ± 0.296
11.32LeuAsn: 11.32 ± 1.618
1.782LeuPro: 1.782 ± 0.379
3.773LeuGln: 3.773 ± 0.686
3.668LeuArg: 3.668 ± 0.559
5.765LeuSer: 5.765 ± 0.616
6.079LeuThr: 6.079 ± 0.874
4.192LeuVal: 4.192 ± 0.635
0.629LeuTrp: 0.629 ± 0.237
2.096LeuTyr: 2.096 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
0.629MetAla: 0.629 ± 0.305
0.105MetCys: 0.105 ± 0.119
1.572MetAsp: 1.572 ± 0.329
0.943MetGlu: 0.943 ± 0.331
1.258MetPhe: 1.258 ± 0.487
0.838MetGly: 0.838 ± 0.292
0.314MetHis: 0.314 ± 0.187
1.153MetIle: 1.153 ± 0.404
2.515MetLys: 2.515 ± 0.474
1.677MetLeu: 1.677 ± 0.379
0.105MetMet: 0.105 ± 0.088
1.782MetAsn: 1.782 ± 0.473
0.838MetPro: 0.838 ± 0.257
1.677MetGln: 1.677 ± 0.356
0.314MetArg: 0.314 ± 0.181
1.467MetSer: 1.467 ± 0.358
0.419MetThr: 0.419 ± 0.241
0.734MetVal: 0.734 ± 0.268
0.314MetTrp: 0.314 ± 0.174
0.314MetTyr: 0.314 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
8.28AsnAla: 8.28 ± 1.13
0.21AsnCys: 0.21 ± 0.148
3.773AsnAsp: 3.773 ± 0.528
7.651AsnGlu: 7.651 ± 0.986
4.192AsnPhe: 4.192 ± 0.901
2.725AsnGly: 2.725 ± 0.565
1.991AsnHis: 1.991 ± 0.415
3.564AsnIle: 3.564 ± 0.566
8.28AsnLys: 8.28 ± 0.686
7.861AsnLeu: 7.861 ± 0.936
1.677AsnMet: 1.677 ± 0.341
6.603AsnAsn: 6.603 ± 0.876
2.201AsnPro: 2.201 ± 0.562
5.031AsnGln: 5.031 ± 0.979
3.144AsnArg: 3.144 ± 0.456
4.088AsnSer: 4.088 ± 0.539
4.088AsnThr: 4.088 ± 0.589
2.306AsnVal: 2.306 ± 0.405
0.419AsnTrp: 0.419 ± 0.189
3.878AsnTyr: 3.878 ± 0.627
0.0AsnXaa: 0.0 ± 0.0
Pro
0.314ProAla: 0.314 ± 0.156
0.0ProCys: 0.0 ± 0.0
0.943ProAsp: 0.943 ± 0.251
1.677ProGlu: 1.677 ± 0.365
1.572ProPhe: 1.572 ± 0.395
0.629ProGly: 0.629 ± 0.286
0.105ProHis: 0.105 ± 0.087
2.306ProIle: 2.306 ± 0.376
3.354ProLys: 3.354 ± 0.57
2.411ProLeu: 2.411 ± 0.512
0.838ProMet: 0.838 ± 0.326
2.725ProAsn: 2.725 ± 0.47
0.524ProPro: 0.524 ± 0.181
0.419ProGln: 0.419 ± 0.166
0.419ProArg: 0.419 ± 0.174
2.515ProSer: 2.515 ± 0.406
1.363ProThr: 1.363 ± 0.376
0.838ProVal: 0.838 ± 0.266
0.0ProTrp: 0.0 ± 0.0
0.838ProTyr: 0.838 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.821GlnAla: 4.821 ± 0.759
0.419GlnCys: 0.419 ± 0.195
1.782GlnAsp: 1.782 ± 0.517
5.345GlnGlu: 5.345 ± 0.936
1.467GlnPhe: 1.467 ± 0.403
2.725GlnGly: 2.725 ± 0.519
0.314GlnHis: 0.314 ± 0.165
2.935GlnIle: 2.935 ± 0.491
5.555GlnLys: 5.555 ± 0.962
3.459GlnLeu: 3.459 ± 0.497
0.838GlnMet: 0.838 ± 0.346
3.144GlnAsn: 3.144 ± 0.461
0.838GlnPro: 0.838 ± 0.243
2.935GlnGln: 2.935 ± 0.663
1.048GlnArg: 1.048 ± 0.367
3.249GlnSer: 3.249 ± 0.65
1.572GlnThr: 1.572 ± 0.329
2.725GlnVal: 2.725 ± 0.549
0.314GlnTrp: 0.314 ± 0.156
0.524GlnTyr: 0.524 ± 0.272
0.0GlnXaa: 0.0 ± 0.0
Arg
2.935ArgAla: 2.935 ± 0.584
0.105ArgCys: 0.105 ± 0.098
2.306ArgAsp: 2.306 ± 0.43
3.354ArgGlu: 3.354 ± 0.543
2.935ArgPhe: 2.935 ± 0.614
1.153ArgGly: 1.153 ± 0.318
0.838ArgHis: 0.838 ± 0.26
2.935ArgIle: 2.935 ± 0.562
3.144ArgLys: 3.144 ± 0.715
5.45ArgLeu: 5.45 ± 0.744
0.524ArgMet: 0.524 ± 0.2
2.096ArgAsn: 2.096 ± 0.423
1.048ArgPro: 1.048 ± 0.354
1.467ArgGln: 1.467 ± 0.489
0.734ArgArg: 0.734 ± 0.283
2.515ArgSer: 2.515 ± 0.513
1.467ArgThr: 1.467 ± 0.582
1.363ArgVal: 1.363 ± 0.388
0.105ArgTrp: 0.105 ± 0.093
1.887ArgTyr: 1.887 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
4.507SerAla: 4.507 ± 0.707
0.524SerCys: 0.524 ± 0.235
4.612SerAsp: 4.612 ± 0.571
6.289SerGlu: 6.289 ± 1.106
3.459SerPhe: 3.459 ± 0.552
3.564SerGly: 3.564 ± 0.62
0.629SerHis: 0.629 ± 0.296
3.459SerIle: 3.459 ± 0.491
5.241SerLys: 5.241 ± 0.557
8.594SerLeu: 8.594 ± 0.8
1.467SerMet: 1.467 ± 0.389
4.192SerAsn: 4.192 ± 0.749
1.363SerPro: 1.363 ± 0.361
2.515SerGln: 2.515 ± 0.483
1.572SerArg: 1.572 ± 0.383
2.62SerSer: 2.62 ± 0.557
1.467SerThr: 1.467 ± 0.371
5.031SerVal: 5.031 ± 0.72
0.314SerTrp: 0.314 ± 0.145
3.04SerTyr: 3.04 ± 0.438
0.0SerXaa: 0.0 ± 0.0
Thr
2.096ThrAla: 2.096 ± 0.518
0.314ThrCys: 0.314 ± 0.172
2.306ThrAsp: 2.306 ± 0.355
3.144ThrGlu: 3.144 ± 0.358
0.838ThrPhe: 0.838 ± 0.331
2.306ThrGly: 2.306 ± 0.489
1.572ThrHis: 1.572 ± 0.529
3.354ThrIle: 3.354 ± 0.599
4.612ThrLys: 4.612 ± 0.885
4.716ThrLeu: 4.716 ± 0.856
1.048ThrMet: 1.048 ± 0.337
3.354ThrAsn: 3.354 ± 0.475
3.04ThrPro: 3.04 ± 0.576
3.459ThrGln: 3.459 ± 0.654
1.467ThrArg: 1.467 ± 0.343
3.564ThrSer: 3.564 ± 0.656
2.935ThrThr: 2.935 ± 0.555
0.524ThrVal: 0.524 ± 0.208
0.524ThrTrp: 0.524 ± 0.191
1.572ThrTyr: 1.572 ± 0.365
0.0ThrXaa: 0.0 ± 0.0
Val
3.04ValAla: 3.04 ± 0.584
0.314ValCys: 0.314 ± 0.182
2.096ValAsp: 2.096 ± 0.391
2.515ValGlu: 2.515 ± 0.404
2.83ValPhe: 2.83 ± 0.539
3.04ValGly: 3.04 ± 0.528
0.314ValHis: 0.314 ± 0.2
3.878ValIle: 3.878 ± 0.628
3.983ValLys: 3.983 ± 0.63
5.241ValLeu: 5.241 ± 0.932
0.734ValMet: 0.734 ± 0.316
2.201ValAsn: 2.201 ± 0.446
0.734ValPro: 0.734 ± 0.254
0.734ValGln: 0.734 ± 0.239
2.096ValArg: 2.096 ± 0.422
3.878ValSer: 3.878 ± 0.782
1.467ValThr: 1.467 ± 0.304
2.201ValVal: 2.201 ± 0.58
0.105ValTrp: 0.105 ± 0.093
1.048ValTyr: 1.048 ± 0.518
0.0ValXaa: 0.0 ± 0.0
Trp
0.105TrpAla: 0.105 ± 0.115
0.0TrpCys: 0.0 ± 0.0
0.21TrpAsp: 0.21 ± 0.137
0.419TrpGlu: 0.419 ± 0.21
0.0TrpPhe: 0.0 ± 0.0
0.314TrpGly: 0.314 ± 0.176
0.105TrpHis: 0.105 ± 0.098
0.419TrpIle: 0.419 ± 0.214
0.314TrpLys: 0.314 ± 0.157
0.105TrpLeu: 0.105 ± 0.122
0.314TrpMet: 0.314 ± 0.192
0.419TrpAsn: 0.419 ± 0.224
0.0TrpPro: 0.0 ± 0.0
0.105TrpGln: 0.105 ± 0.093
0.314TrpArg: 0.314 ± 0.142
0.419TrpSer: 0.419 ± 0.249
0.21TrpThr: 0.21 ± 0.134
0.734TrpVal: 0.734 ± 0.286
0.0TrpTrp: 0.0 ± 0.0
0.21TrpTyr: 0.21 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.572TyrAla: 1.572 ± 0.425
0.314TyrCys: 0.314 ± 0.183
1.782TyrAsp: 1.782 ± 0.338
3.04TyrGlu: 3.04 ± 0.524
1.991TyrPhe: 1.991 ± 0.613
1.153TyrGly: 1.153 ± 0.241
0.734TyrHis: 0.734 ± 0.267
2.725TyrIle: 2.725 ± 0.545
4.192TyrLys: 4.192 ± 0.533
3.564TyrLeu: 3.564 ± 0.591
0.524TyrMet: 0.524 ± 0.232
2.83TyrAsn: 2.83 ± 0.417
1.258TyrPro: 1.258 ± 0.303
2.515TyrGln: 2.515 ± 0.412
1.467TyrArg: 1.467 ± 0.349
2.515TyrSer: 2.515 ± 0.443
1.363TyrThr: 1.363 ± 0.323
0.314TyrVal: 0.314 ± 0.179
0.0TyrTrp: 0.0 ± 0.0
1.258TyrTyr: 1.258 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (9542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski