Devosia sp. I507
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3776 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L1S497|A0A2L1S497_9RHIZ N-acylglucosamine-6-phosphate 2-epimerase OS=Devosia sp. I507 OX=2083786 GN=C4375_05615 PE=4 SV=1
MM1 pKa = 7.51 FFPSDD6 pKa = 3.75 PLSEE10 pKa = 4.14 IVAHH14 pKa = 5.98 FLGYY18 pKa = 9.4 FQIAVEE24 pKa = 4.04 DD25 pKa = 3.52 MKK27 pKa = 11.39 VRR29 pKa = 11.84 LEE31 pKa = 3.88 MALAEE36 pKa = 4.45 HH37 pKa = 6.45 EE38 pKa = 4.41 AEE40 pKa = 4.97 AGLEE44 pKa = 4.03 APEE47 pKa = 4.03 MQQVSVDD54 pKa = 3.71 VLQTYY59 pKa = 11.04 ALGNYY64 pKa = 9.57 APGVFYY70 pKa = 10.74 APPEE74 pKa = 3.72 FLIRR78 pKa = 11.84 GEE80 pKa = 4.37 VMFGPTNIGLANTPDD95 pKa = 3.72 FTASEE100 pKa = 4.34 LDD102 pKa = 3.66 SPLSQWPTGPGDD114 pKa = 3.48 IVIPLIGTEE123 pKa = 4.15 PGSVIAVFSQTISLSDD139 pKa = 3.62 DD140 pKa = 3.46 DD141 pKa = 4.7 VFVMGTLDD149 pKa = 3.66 EE150 pKa = 4.54 PLVYY154 pKa = 10.44 QSGSDD159 pKa = 3.34 AGLLGLQAASNAILGPIGSGPGMTSHH185 pKa = 7.0 GDD187 pKa = 3.21 VPLVMDD193 pKa = 5.24 AVHH196 pKa = 6.35 ATAKK200 pKa = 10.15 AHH202 pKa = 5.89 QSNPDD207 pKa = 3.34 TQFEE211 pKa = 4.36 QVEE214 pKa = 4.86 SLTGTYY220 pKa = 10.95 VNGKK224 pKa = 9.63 LEE226 pKa = 4.22 TEE228 pKa = 4.09 APEE231 pKa = 4.27 LTDD234 pKa = 4.43 ALPSHH239 pKa = 6.6 LQAEE243 pKa = 4.9 AEE245 pKa = 4.35 DD246 pKa = 4.68 APPPDD251 pKa = 4.19 TIKK254 pKa = 9.94 VTSEE258 pKa = 4.0 HH259 pKa = 6.36 EE260 pKa = 3.68 AHH262 pKa = 6.4 IQIEE266 pKa = 4.1 NDD268 pKa = 2.96 ATQGSVTYY276 pKa = 10.62 NSGGNFLVNEE286 pKa = 4.04 AVVLNGGLVGTHH298 pKa = 5.85 FVVGGNFHH306 pKa = 6.34 QLDD309 pKa = 4.78 AIIQTNAFSDD319 pKa = 3.51 TDD321 pKa = 3.85 YY322 pKa = 11.74 VNDD325 pKa = 5.1 DD326 pKa = 4.13 FPLDD330 pKa = 3.61 AQAGGGTKK338 pKa = 10.07 AVNVASFVQEE348 pKa = 4.1 TFDD351 pKa = 4.08 AAGDD355 pKa = 3.74 NAEE358 pKa = 4.57 ANPGVMPQNWQVTCVSGDD376 pKa = 3.68 VIFLEE381 pKa = 4.43 WMKK384 pKa = 10.85 QLTFMSDD391 pKa = 2.73 EE392 pKa = 4.28 DD393 pKa = 4.24 VGVLTATGSDD403 pKa = 3.12 AMVTSGEE410 pKa = 4.23 NIGLNSVNFANIGSLFDD427 pKa = 5.1 LILVGGNVFDD437 pKa = 5.81 ANVIVQNNILYY448 pKa = 10.67 DD449 pKa = 3.7 NDD451 pKa = 4.5 TITMLGAGDD460 pKa = 4.62 DD461 pKa = 4.13 GDD463 pKa = 4.2 YY464 pKa = 10.96 SASGSLNTSNNLLWNQASIHH484 pKa = 5.78 NVGATEE490 pKa = 4.16 VKK492 pKa = 10.52 SGVSDD497 pKa = 3.8 HH498 pKa = 6.87 YY499 pKa = 11.07 KK500 pKa = 10.92 DD501 pKa = 5.41 AMDD504 pKa = 5.06 GLANGNKK511 pKa = 9.86 SMPASFYY518 pKa = 10.53 AAEE521 pKa = 4.46 DD522 pKa = 4.02 FEE524 pKa = 6.13 GIAIPRR530 pKa = 11.84 VLFVSGSIYY539 pKa = 10.05 DD540 pKa = 3.51 LRR542 pKa = 11.84 YY543 pKa = 9.69 IEE545 pKa = 4.06 QTNILGDD552 pKa = 3.72 ADD554 pKa = 4.71 FVALQKK560 pKa = 10.89 KK561 pKa = 9.88 AFLDD565 pKa = 4.1 GEE567 pKa = 4.58 PATEE571 pKa = 4.01 WNIDD575 pKa = 3.45 TGSNALVNMAAIKK588 pKa = 10.58 DD589 pKa = 3.82 YY590 pKa = 9.86 DD591 pKa = 3.83 TFGDD595 pKa = 4.07 SIEE598 pKa = 4.37 VGGDD602 pKa = 3.18 HH603 pKa = 6.77 YY604 pKa = 11.49 SDD606 pKa = 5.3 SILIQANILEE616 pKa = 4.43 AQGDD620 pKa = 4.04 GDD622 pKa = 5.57 DD623 pKa = 5.34 DD624 pKa = 4.4 DD625 pKa = 6.61 ALVSEE630 pKa = 4.99 VVAFLDD636 pKa = 3.62 HH637 pKa = 6.94 TEE639 pKa = 4.2 TLADD643 pKa = 3.81 TPDD646 pKa = 3.44 YY647 pKa = 11.0 SALGDD652 pKa = 3.92 TTHH655 pKa = 7.69 DD656 pKa = 3.77 AAPVDD661 pKa = 4.09 LMQSVLAA668 pKa = 4.92
Molecular weight: 70.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.465
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.05
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.821
Patrickios 1.43
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A2L1SCC4|A0A2L1SCC4_9RHIZ Choline phosphatase OS=Devosia sp. I507 OX=2083786 GN=cls PE=4 SV=1
MM1 pKa = 6.91 TRR3 pKa = 11.84 APGRR7 pKa = 11.84 PWSRR11 pKa = 11.84 PRR13 pKa = 11.84 TLRR16 pKa = 11.84 PPRR19 pKa = 11.84 LRR21 pKa = 11.84 TSRR24 pKa = 11.84 PARR27 pKa = 11.84 PRR29 pKa = 11.84 PRR31 pKa = 11.84 HH32 pKa = 4.72 RR33 pKa = 11.84 CQRR36 pKa = 11.84 RR37 pKa = 11.84 WQRR40 pKa = 11.84 PTPRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 LWQSRR51 pKa = 11.84 HH52 pKa = 4.56 RR53 pKa = 11.84 PRR55 pKa = 11.84 PKK57 pKa = 9.22 RR58 pKa = 11.84 WPVGPEE64 pKa = 3.2 PGRR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 SHH73 pKa = 7.19 DD74 pKa = 3.45 CLLRR78 pKa = 11.84 SPWFRR83 pKa = 11.84 LARR86 pKa = 11.84 QPPRR90 pKa = 11.84 PRR92 pKa = 11.84 RR93 pKa = 11.84 GG94 pKa = 3.01
Molecular weight: 11.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 10.979
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.266
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 11.974
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.156
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3776
0
3776
1170282
41
2904
309.9
33.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.297 ± 0.058
0.692 ± 0.011
5.796 ± 0.038
5.752 ± 0.037
3.834 ± 0.026
8.55 ± 0.036
2.028 ± 0.019
5.488 ± 0.027
2.946 ± 0.033
10.36 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.02
2.801 ± 0.027
5.038 ± 0.03
3.34 ± 0.023
6.492 ± 0.04
5.427 ± 0.029
5.46 ± 0.024
7.502 ± 0.035
1.344 ± 0.013
2.247 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here