Caulobacter vibrioides (Caulobacter crescentus)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V2F7K9|A0A0V2F7K9_CAUVI Pectate lyase OS=Caulobacter vibrioides OX=155892 GN=AS593_23375 PE=4 SV=1
MM1 pKa = 7.36 SLSSALSTAVSGLTAQSIALSAISEE26 pKa = 4.39 NIANSSTTAYY36 pKa = 7.44 KK37 pKa = 7.9 TTEE40 pKa = 3.72 VSFQSLVSGAMGSNTSTSSVVAKK63 pKa = 9.72 TNQALNVTGTIASTSVATNIAIDD86 pKa = 3.65 GAGYY90 pKa = 9.52 FVVAGAPGDD99 pKa = 3.75 TGSDD103 pKa = 2.88 LVYY106 pKa = 10.77 SRR108 pKa = 11.84 NGSFSTDD115 pKa = 2.62 SDD117 pKa = 4.22 GYY119 pKa = 10.56 LVNSEE124 pKa = 4.58 GYY126 pKa = 10.17 YY127 pKa = 11.18 LMGFPTDD134 pKa = 3.32 ADD136 pKa = 4.11 GNILSSNTSDD146 pKa = 3.48 LTSLSAINLDD156 pKa = 4.5 SISGTAKK163 pKa = 9.49 ATSTVTMAANLPADD177 pKa = 3.76 ATTGQTFTTSMEE189 pKa = 4.44 VIDD192 pKa = 4.32 SLGVSHH198 pKa = 7.03 TVGQSWTKK206 pKa = 10.39 TGDD209 pKa = 3.67 NTWSLTLSDD218 pKa = 4.49 PVLTKK223 pKa = 10.86 DD224 pKa = 3.34 GATTSGTISPSTVTVTFNSDD244 pKa = 2.52 GSLATTDD251 pKa = 4.14 PSPVAIGITGLSTGAADD268 pKa = 4.53 SAITLDD274 pKa = 4.25 LGDD277 pKa = 4.62 AGGTDD282 pKa = 3.84 GLTQFASTSSTLSISPTFEE301 pKa = 3.5 QDD303 pKa = 2.87 GANYY307 pKa = 9.34 GQLSGITIDD316 pKa = 3.99 GNGLVTANFDD326 pKa = 3.68 NGVSLAIYY334 pKa = 7.7 QIPVATFSNPNGLTHH349 pKa = 7.2 VSGTVYY355 pKa = 10.89 DD356 pKa = 4.21 EE357 pKa = 4.08 NTNAGQVHH365 pKa = 6.94 LNLPGDD371 pKa = 4.24 GSAGSLVASALEE383 pKa = 4.34 GSTTDD388 pKa = 4.07 IATEE392 pKa = 3.96 FNKK395 pKa = 10.21 MIIAQQAYY403 pKa = 8.5 SAASQVVSASSDD415 pKa = 3.31 MFDD418 pKa = 3.52 TLIQAVRR425 pKa = 3.38
Molecular weight: 42.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.579
IPC_protein 3.617
Toseland 3.376
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.287
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 4.05
Thurlkill 3.452
EMBOSS 3.617
Sillero 3.745
Patrickios 1.062
IPC_peptide 3.617
IPC2_peptide 3.719
IPC2.peptide.svr19 3.687
Protein with the highest isoelectric point:
>tr|A0A0V2FF12|A0A0V2FF12_CAUVI 6 7-dimethyl-8-ribityllumazine synthase OS=Caulobacter vibrioides OX=155892 GN=ribH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.58 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.26 GYY18 pKa = 7.96 RR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 9.84 NGQKK29 pKa = 9.53 IVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4642
0
4642
1543440
29
4428
332.5
35.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.189 ± 0.063
0.672 ± 0.009
5.884 ± 0.025
5.308 ± 0.028
3.538 ± 0.02
9.224 ± 0.062
1.683 ± 0.015
4.126 ± 0.023
3.306 ± 0.029
10.021 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.043 ± 0.016
2.371 ± 0.027
5.483 ± 0.035
3.109 ± 0.019
7.07 ± 0.038
5.201 ± 0.033
5.354 ± 0.037
7.738 ± 0.026
1.45 ± 0.017
2.231 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here