Bos taurus papillomavirus 2 (Bovine papillomavirus 2)
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5PW87|S5PW87_BPV2 Protein E7 OS=Bos taurus papillomavirus 2 OX=2758382 GN=E7 PE=3 SV=1
MM1 pKa = 7.49 PNLWFLLFLGLVAAMQLLLLLFLLLFFLVYY31 pKa = 9.25 WDD33 pKa = 4.88 HH34 pKa = 7.04 FEE36 pKa = 4.31 CSCTGLPFF44 pKa = 4.63
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.503
IPC2_protein 4.876
IPC_protein 4.151
Toseland 4.062
ProMoST 4.393
Dawson 4.177
Bjellqvist 4.355
Wikipedia 4.177
Rodwell 4.05
Grimsley 4.012
Solomon 4.101
Lehninger 4.062
Nozaki 4.38
DTASelect 4.431
Thurlkill 4.19
EMBOSS 4.177
Sillero 4.304
Patrickios 0.006
IPC_peptide 4.113
IPC2_peptide 4.279
IPC2.peptide.svr19 4.237
Protein with the highest isoelectric point:
>tr|A0A159ZTD8|A0A159ZTD8_BPV2 Protein E6 OS=Bos taurus papillomavirus 2 OX=2758382 GN=E6 PE=3 SV=1
MM1 pKa = 7.21 LVSHH5 pKa = 7.33 PPLLILEE12 pKa = 4.73 IAQTEE17 pKa = 4.58 SGSHH21 pKa = 4.82 QKK23 pKa = 10.32 DD24 pKa = 3.33 LKK26 pKa = 10.22 EE27 pKa = 3.93 TLQEE31 pKa = 4.37 KK32 pKa = 10.44 KK33 pKa = 10.02 PSQPSLSLLCSAPPPAVPSEE53 pKa = 4.08 QASVGYY59 pKa = 8.37 GTVLARR65 pKa = 11.84 TPTIFLQARR74 pKa = 11.84 GALFSALPPPRR85 pKa = 11.84 CRR87 pKa = 11.84 ARR89 pKa = 11.84 YY90 pKa = 8.6 RR91 pKa = 11.84 WTWHH95 pKa = 4.96 QGRR98 pKa = 11.84 KK99 pKa = 8.92 KK100 pKa = 10.79 KK101 pKa = 10.62 NINRR105 pKa = 11.84 PTQQRR110 pKa = 11.84 RR111 pKa = 11.84 NLL113 pKa = 3.56
Molecular weight: 12.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.765
IPC_protein 10.584
Toseland 10.935
ProMoST 10.657
Dawson 10.994
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.257
Grimsley 11.038
Solomon 11.169
Lehninger 11.125
Nozaki 10.921
DTASelect 10.716
Thurlkill 10.921
EMBOSS 11.345
Sillero 10.935
Patrickios 11.008
IPC_peptide 11.169
IPC2_peptide 9.78
IPC2.peptide.svr19 8.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2479
44
606
275.4
30.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.624 ± 0.617
2.501 ± 0.695
5.002 ± 0.406
6.01 ± 0.522
4.155 ± 0.651
7.664 ± 0.771
2.42 ± 0.208
3.59 ± 0.587
5.284 ± 0.646
10.367 ± 1.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.533 ± 0.344
3.59 ± 0.476
6.01 ± 1.111
4.155 ± 0.515
5.889 ± 0.53
7.503 ± 0.466
6.616 ± 0.588
5.607 ± 0.306
1.493 ± 0.22
2.985 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here