Lactococcus phage bIL312

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZE9|Q9AZE9_9CAUD Orf11 OS=Lactococcus phage bIL312 OX=151539 GN=orf11 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 9.41ITIDD6 pKa = 3.51VLEE9 pKa = 4.58NEE11 pKa = 4.49SNKK14 pKa = 10.85DD15 pKa = 3.29NLEE18 pKa = 3.92YY19 pKa = 10.73LISDD23 pKa = 4.45TSNEE27 pKa = 4.32AITVLMFALIGEE39 pKa = 4.42ARR41 pKa = 11.84QRR43 pKa = 11.84ASYY46 pKa = 9.96EE47 pKa = 3.94QFLEE51 pKa = 4.54TITRR55 pKa = 11.84IWGYY59 pKa = 8.96MNEE62 pKa = 4.34DD63 pKa = 3.19NN64 pKa = 5.33

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZD5|Q9AZD5_9CAUD Orf25 OS=Lactococcus phage bIL312 OX=151539 GN=orf25 PE=4 SV=1
MM1 pKa = 7.27LTTRR5 pKa = 11.84EE6 pKa = 3.93QYY8 pKa = 10.84RR9 pKa = 11.84IMKK12 pKa = 10.12LLTKK16 pKa = 9.81HH17 pKa = 5.71YY18 pKa = 9.17KK19 pKa = 9.45SSKK22 pKa = 7.79ITLKK26 pKa = 10.54ILLGRR31 pKa = 11.84VTVGSSCLPQVILRR45 pKa = 11.84SGFFYY50 pKa = 10.44IKK52 pKa = 10.09KK53 pKa = 10.28ALALSQCC60 pKa = 4.01

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

3799

46

480

140.7

16.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.317 ± 0.366

0.553 ± 0.107

6.344 ± 0.378

7.923 ± 0.581

4.896 ± 0.396

4.685 ± 0.456

1.843 ± 0.262

7.555 ± 0.511

10.634 ± 0.561

8.976 ± 0.44

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.764 ± 0.251

6.475 ± 0.357

2.29 ± 0.207

3.764 ± 0.355

3.659 ± 0.378

6.37 ± 0.502

5.238 ± 0.386

5.159 ± 0.296

1.158 ± 0.2

4.396 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski