Amino acid dipepetide frequency for Lactococcus phage bIL312

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.053AlaAla: 1.053 ± 0.576
0.263AlaCys: 0.263 ± 0.254
2.896AlaAsp: 2.896 ± 0.857
5.266AlaGlu: 5.266 ± 1.179
2.106AlaPhe: 2.106 ± 0.715
2.106AlaGly: 2.106 ± 1.09
1.58AlaHis: 1.58 ± 0.591
6.319AlaIle: 6.319 ± 1.188
6.846AlaLys: 6.846 ± 1.492
5.003AlaLeu: 5.003 ± 1.254
1.58AlaMet: 1.58 ± 0.561
2.37AlaAsn: 2.37 ± 0.769
1.316AlaPro: 1.316 ± 0.601
1.58AlaGln: 1.58 ± 0.621
2.106AlaArg: 2.106 ± 0.783
2.106AlaSer: 2.106 ± 0.722
2.106AlaThr: 2.106 ± 0.827
3.423AlaVal: 3.423 ± 0.799
0.79AlaTrp: 0.79 ± 0.566
2.37AlaTyr: 2.37 ± 0.714
0.0AlaXaa: 0.0 ± 0.0
Cys
0.263CysAla: 0.263 ± 0.299
0.263CysCys: 0.263 ± 0.262
1.053CysAsp: 1.053 ± 0.487
0.0CysGlu: 0.0 ± 0.0
0.263CysPhe: 0.263 ± 0.222
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.263CysIle: 0.263 ± 0.296
0.0CysLys: 0.0 ± 0.0
0.527CysLeu: 0.527 ± 0.368
0.263CysMet: 0.263 ± 0.291
0.263CysAsn: 0.263 ± 0.262
0.527CysPro: 0.527 ± 0.301
0.0CysGln: 0.0 ± 0.0
0.79CysArg: 0.79 ± 0.387
0.263CysSer: 0.263 ± 0.223
0.527CysThr: 0.527 ± 0.434
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.263CysTyr: 0.263 ± 0.223
0.0CysXaa: 0.0 ± 0.0
Asp
1.843AspAla: 1.843 ± 0.629
0.79AspCys: 0.79 ± 0.334
6.582AspAsp: 6.582 ± 1.533
4.739AspGlu: 4.739 ± 0.994
3.686AspPhe: 3.686 ± 1.024
2.896AspGly: 2.896 ± 0.731
0.263AspHis: 0.263 ± 0.299
3.686AspIle: 3.686 ± 0.905
7.109AspLys: 7.109 ± 1.501
5.793AspLeu: 5.793 ± 1.281
2.106AspMet: 2.106 ± 0.824
4.213AspAsn: 4.213 ± 1.468
1.053AspPro: 1.053 ± 0.491
2.106AspGln: 2.106 ± 0.653
2.106AspArg: 2.106 ± 0.567
3.949AspSer: 3.949 ± 1.074
2.106AspThr: 2.106 ± 0.88
3.423AspVal: 3.423 ± 0.955
0.527AspTrp: 0.527 ± 0.379
4.476AspTyr: 4.476 ± 0.888
0.0AspXaa: 0.0 ± 0.0
Glu
4.739GluAla: 4.739 ± 1.077
0.263GluCys: 0.263 ± 0.252
3.423GluAsp: 3.423 ± 1.03
6.582GluGlu: 6.582 ± 1.587
4.213GluPhe: 4.213 ± 0.948
3.423GluGly: 3.423 ± 1.338
1.053GluHis: 1.053 ± 0.493
6.846GluIle: 6.846 ± 1.333
9.215GluLys: 9.215 ± 1.888
7.636GluLeu: 7.636 ± 1.661
3.16GluMet: 3.16 ± 0.831
5.529GluAsn: 5.529 ± 1.281
0.263GluPro: 0.263 ± 0.299
4.213GluGln: 4.213 ± 1.095
3.423GluArg: 3.423 ± 0.857
2.633GluSer: 2.633 ± 0.718
4.213GluThr: 4.213 ± 1.116
3.423GluVal: 3.423 ± 0.833
1.053GluTrp: 1.053 ± 0.687
3.949GluTyr: 3.949 ± 0.848
0.0GluXaa: 0.0 ± 0.0
Phe
2.37PheAla: 2.37 ± 0.799
0.0PheCys: 0.0 ± 0.0
5.003PheAsp: 5.003 ± 1.003
5.266PheGlu: 5.266 ± 1.445
1.843PhePhe: 1.843 ± 0.559
1.843PheGly: 1.843 ± 0.781
0.263PheHis: 0.263 ± 0.292
4.213PheIle: 4.213 ± 0.874
4.213PheLys: 4.213 ± 1.024
5.266PheLeu: 5.266 ± 1.325
1.053PheMet: 1.053 ± 0.418
4.213PheAsn: 4.213 ± 1.024
0.79PhePro: 0.79 ± 0.388
1.053PheGln: 1.053 ± 0.468
2.106PheArg: 2.106 ± 0.664
2.633PheSer: 2.633 ± 0.868
2.106PheThr: 2.106 ± 0.821
2.633PheVal: 2.633 ± 1.071
0.263PheTrp: 0.263 ± 0.252
1.843PheTyr: 1.843 ± 0.606
0.0PheXaa: 0.0 ± 0.0
Gly
1.58GlyAla: 1.58 ± 0.882
0.527GlyCys: 0.527 ± 0.345
1.053GlyAsp: 1.053 ± 0.527
3.686GlyGlu: 3.686 ± 0.979
3.423GlyPhe: 3.423 ± 1.063
2.633GlyGly: 2.633 ± 0.928
1.316GlyHis: 1.316 ± 0.527
3.16GlyIle: 3.16 ± 0.876
3.686GlyLys: 3.686 ± 0.954
3.949GlyLeu: 3.949 ± 0.976
1.843GlyMet: 1.843 ± 0.622
2.633GlyAsn: 2.633 ± 1.032
0.527GlyPro: 0.527 ± 0.344
1.843GlyGln: 1.843 ± 0.743
1.316GlyArg: 1.316 ± 0.644
2.37GlySer: 2.37 ± 0.722
3.423GlyThr: 3.423 ± 0.801
3.16GlyVal: 3.16 ± 0.683
0.527GlyTrp: 0.527 ± 0.446
4.213GlyTyr: 4.213 ± 0.929
0.0GlyXaa: 0.0 ± 0.0
His
0.0HisAla: 0.0 ± 0.0
0.263HisCys: 0.263 ± 0.223
1.316HisAsp: 1.316 ± 0.598
1.58HisGlu: 1.58 ± 0.862
1.58HisPhe: 1.58 ± 0.582
0.79HisGly: 0.79 ± 0.432
0.263HisHis: 0.263 ± 0.284
1.58HisIle: 1.58 ± 0.61
1.843HisLys: 1.843 ± 0.711
2.37HisLeu: 2.37 ± 1.059
0.0HisMet: 0.0 ± 0.0
0.79HisAsn: 0.79 ± 0.39
1.053HisPro: 1.053 ± 0.687
1.053HisGln: 1.053 ± 0.481
0.0HisArg: 0.0 ± 0.0
0.0HisSer: 0.0 ± 0.0
1.58HisThr: 1.58 ± 0.786
1.053HisVal: 1.053 ± 0.469
0.527HisTrp: 0.527 ± 0.39
0.79HisTyr: 0.79 ± 0.455
0.0HisXaa: 0.0 ± 0.0
Ile
4.213IleAla: 4.213 ± 0.839
0.527IleCys: 0.527 ± 0.334
5.266IleAsp: 5.266 ± 1.581
6.846IleGlu: 6.846 ± 1.109
2.37IlePhe: 2.37 ± 0.806
3.686IleGly: 3.686 ± 1.078
0.79IleHis: 0.79 ± 0.421
5.003IleIle: 5.003 ± 1.367
7.899IleLys: 7.899 ± 1.547
6.582IleLeu: 6.582 ± 1.546
1.843IleMet: 1.843 ± 0.644
5.793IleAsn: 5.793 ± 1.521
1.58IlePro: 1.58 ± 0.735
2.37IleGln: 2.37 ± 0.792
3.686IleArg: 3.686 ± 1.247
5.793IleSer: 5.793 ± 1.185
5.793IleThr: 5.793 ± 1.578
2.633IleVal: 2.633 ± 0.779
0.527IleTrp: 0.527 ± 0.438
2.37IleTyr: 2.37 ± 0.74
0.0IleXaa: 0.0 ± 0.0
Lys
7.372LysAla: 7.372 ± 1.108
0.0LysCys: 0.0 ± 0.0
4.739LysAsp: 4.739 ± 0.859
8.689LysGlu: 8.689 ± 1.007
3.949LysPhe: 3.949 ± 1.337
4.213LysGly: 4.213 ± 1.216
2.37LysHis: 2.37 ± 0.991
9.479LysIle: 9.479 ± 1.595
10.532LysLys: 10.532 ± 1.927
7.109LysLeu: 7.109 ± 0.82
2.896LysMet: 2.896 ± 0.83
9.742LysAsn: 9.742 ± 2.026
3.686LysPro: 3.686 ± 1.224
4.213LysGln: 4.213 ± 1.1
3.423LysArg: 3.423 ± 0.862
6.846LysSer: 6.846 ± 1.225
3.949LysThr: 3.949 ± 1.184
6.056LysVal: 6.056 ± 1.314
1.316LysTrp: 1.316 ± 0.56
5.793LysTyr: 5.793 ± 0.972
0.0LysXaa: 0.0 ± 0.0
Leu
5.266LeuAla: 5.266 ± 1.272
0.79LeuCys: 0.79 ± 0.508
6.319LeuAsp: 6.319 ± 1.025
8.425LeuGlu: 8.425 ± 1.499
5.529LeuPhe: 5.529 ± 1.227
2.896LeuGly: 2.896 ± 0.863
0.79LeuHis: 0.79 ± 0.413
5.529LeuIle: 5.529 ± 1.348
8.689LeuLys: 8.689 ± 1.36
8.162LeuLeu: 8.162 ± 1.628
2.633LeuMet: 2.633 ± 0.901
6.582LeuAsn: 6.582 ± 0.998
1.58LeuPro: 1.58 ± 0.574
3.686LeuGln: 3.686 ± 0.952
2.37LeuArg: 2.37 ± 0.897
8.425LeuSer: 8.425 ± 1.36
5.266LeuThr: 5.266 ± 1.271
3.423LeuVal: 3.423 ± 0.998
0.79LeuTrp: 0.79 ± 0.431
2.633LeuTyr: 2.633 ± 0.801
0.0LeuXaa: 0.0 ± 0.0
Met
3.16MetAla: 3.16 ± 0.884
0.0MetCys: 0.0 ± 0.0
0.527MetAsp: 0.527 ± 0.354
2.106MetGlu: 2.106 ± 0.771
0.527MetPhe: 0.527 ± 0.457
2.106MetGly: 2.106 ± 0.577
0.79MetHis: 0.79 ± 0.415
2.896MetIle: 2.896 ± 0.987
3.686MetLys: 3.686 ± 1.135
1.58MetLeu: 1.58 ± 0.639
0.79MetMet: 0.79 ± 0.634
1.58MetAsn: 1.58 ± 0.618
0.79MetPro: 0.79 ± 0.436
1.053MetGln: 1.053 ± 0.536
0.263MetArg: 0.263 ± 0.29
2.106MetSer: 2.106 ± 0.762
2.106MetThr: 2.106 ± 0.496
0.79MetVal: 0.79 ± 0.39
0.527MetTrp: 0.527 ± 0.354
0.263MetTyr: 0.263 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
3.686AsnAla: 3.686 ± 0.735
0.0AsnCys: 0.0 ± 0.0
3.16AsnAsp: 3.16 ± 0.851
5.003AsnGlu: 5.003 ± 1.556
3.16AsnPhe: 3.16 ± 0.698
3.16AsnGly: 3.16 ± 0.748
1.053AsnHis: 1.053 ± 0.419
4.739AsnIle: 4.739 ± 1.255
8.952AsnLys: 8.952 ± 1.26
3.949AsnLeu: 3.949 ± 1.109
2.633AsnMet: 2.633 ± 0.788
4.213AsnAsn: 4.213 ± 1.069
1.843AsnPro: 1.843 ± 0.584
3.423AsnGln: 3.423 ± 0.839
0.79AsnArg: 0.79 ± 0.429
4.739AsnSer: 4.739 ± 0.886
2.106AsnThr: 2.106 ± 0.701
4.739AsnVal: 4.739 ± 0.989
1.053AsnTrp: 1.053 ± 0.514
2.106AsnTyr: 2.106 ± 0.499
0.0AsnXaa: 0.0 ± 0.0
Pro
1.58ProAla: 1.58 ± 0.811
0.0ProCys: 0.0 ± 0.0
0.79ProAsp: 0.79 ± 0.397
0.263ProGlu: 0.263 ± 0.29
1.58ProPhe: 1.58 ± 0.714
0.0ProGly: 0.0 ± 0.0
0.263ProHis: 0.263 ± 0.223
1.58ProIle: 1.58 ± 0.618
2.896ProLys: 2.896 ± 0.818
2.106ProLeu: 2.106 ± 0.816
0.79ProMet: 0.79 ± 0.511
1.58ProAsn: 1.58 ± 0.61
0.527ProPro: 0.527 ± 0.361
1.316ProGln: 1.316 ± 0.514
1.316ProArg: 1.316 ± 0.507
0.79ProSer: 0.79 ± 0.464
2.37ProThr: 2.37 ± 0.605
2.37ProVal: 2.37 ± 0.789
0.0ProTrp: 0.0 ± 0.0
0.79ProTyr: 0.79 ± 0.447
0.0ProXaa: 0.0 ± 0.0
Gln
1.843GlnAla: 1.843 ± 0.752
0.527GlnCys: 0.527 ± 0.385
1.316GlnAsp: 1.316 ± 0.503
2.37GlnGlu: 2.37 ± 0.897
1.843GlnPhe: 1.843 ± 0.526
3.949GlnGly: 3.949 ± 1.135
0.527GlnHis: 0.527 ± 0.394
1.843GlnIle: 1.843 ± 0.725
5.003GlnLys: 5.003 ± 0.953
3.423GlnLeu: 3.423 ± 0.746
0.0GlnMet: 0.0 ± 0.0
2.37GlnAsn: 2.37 ± 0.704
0.527GlnPro: 0.527 ± 0.362
1.053GlnGln: 1.053 ± 0.499
1.316GlnArg: 1.316 ± 0.627
3.949GlnSer: 3.949 ± 0.729
1.843GlnThr: 1.843 ± 0.516
2.106GlnVal: 2.106 ± 0.746
0.0GlnTrp: 0.0 ± 0.0
1.843GlnTyr: 1.843 ± 0.639
0.0GlnXaa: 0.0 ± 0.0
Arg
3.16ArgAla: 3.16 ± 0.885
0.0ArgCys: 0.0 ± 0.0
1.053ArgAsp: 1.053 ± 0.496
1.58ArgGlu: 1.58 ± 0.607
1.053ArgPhe: 1.053 ± 0.552
2.633ArgGly: 2.633 ± 0.837
1.316ArgHis: 1.316 ± 0.598
2.106ArgIle: 2.106 ± 0.751
2.106ArgLys: 2.106 ± 0.508
3.949ArgLeu: 3.949 ± 0.996
1.58ArgMet: 1.58 ± 0.534
1.316ArgAsn: 1.316 ± 0.49
1.316ArgPro: 1.316 ± 0.511
1.316ArgGln: 1.316 ± 0.537
1.316ArgArg: 1.316 ± 0.548
3.423ArgSer: 3.423 ± 0.705
2.896ArgThr: 2.896 ± 0.816
1.58ArgVal: 1.58 ± 0.556
0.527ArgTrp: 0.527 ± 0.401
2.37ArgTyr: 2.37 ± 0.696
0.0ArgXaa: 0.0 ± 0.0
Ser
2.37SerAla: 2.37 ± 0.742
0.263SerCys: 0.263 ± 0.307
5.266SerAsp: 5.266 ± 0.965
5.529SerGlu: 5.529 ± 1.145
4.476SerPhe: 4.476 ± 0.859
2.37SerGly: 2.37 ± 0.703
2.106SerHis: 2.106 ± 0.665
3.949SerIle: 3.949 ± 1.162
7.372SerLys: 7.372 ± 1.335
5.793SerLeu: 5.793 ± 1.078
0.527SerMet: 0.527 ± 0.446
3.949SerAsn: 3.949 ± 1.169
2.106SerPro: 2.106 ± 0.76
1.316SerGln: 1.316 ± 0.614
2.633SerArg: 2.633 ± 0.721
3.423SerSer: 3.423 ± 1.512
2.37SerThr: 2.37 ± 1.047
4.213SerVal: 4.213 ± 1.159
1.316SerTrp: 1.316 ± 0.528
2.37SerTyr: 2.37 ± 0.645
0.0SerXaa: 0.0 ± 0.0
Thr
3.16ThrAla: 3.16 ± 0.694
0.79ThrCys: 0.79 ± 0.506
2.896ThrAsp: 2.896 ± 0.764
3.686ThrGlu: 3.686 ± 0.946
1.316ThrPhe: 1.316 ± 0.723
2.633ThrGly: 2.633 ± 1.096
1.053ThrHis: 1.053 ± 0.432
3.16ThrIle: 3.16 ± 0.957
6.056ThrLys: 6.056 ± 1.279
6.319ThrLeu: 6.319 ± 1.258
0.79ThrMet: 0.79 ± 0.43
2.37ThrAsn: 2.37 ± 0.627
1.843ThrPro: 1.843 ± 0.65
2.37ThrGln: 2.37 ± 0.825
2.633ThrArg: 2.633 ± 0.73
3.16ThrSer: 3.16 ± 0.921
3.16ThrThr: 3.16 ± 0.733
3.686ThrVal: 3.686 ± 0.84
0.263ThrTrp: 0.263 ± 0.328
1.053ThrTyr: 1.053 ± 0.498
0.0ThrXaa: 0.0 ± 0.0
Val
3.686ValAla: 3.686 ± 0.957
0.0ValCys: 0.0 ± 0.0
6.056ValAsp: 6.056 ± 1.088
3.423ValGlu: 3.423 ± 1.046
1.843ValPhe: 1.843 ± 0.667
2.633ValGly: 2.633 ± 0.639
1.58ValHis: 1.58 ± 0.721
5.266ValIle: 5.266 ± 1.344
3.949ValLys: 3.949 ± 0.911
3.949ValLeu: 3.949 ± 1.097
1.843ValMet: 1.843 ± 0.576
1.58ValAsn: 1.58 ± 0.707
1.053ValPro: 1.053 ± 0.521
1.316ValGln: 1.316 ± 0.595
2.896ValArg: 2.896 ± 0.902
3.423ValSer: 3.423 ± 1.008
2.106ValThr: 2.106 ± 0.672
1.58ValVal: 1.58 ± 0.702
0.527ValTrp: 0.527 ± 0.352
2.896ValTyr: 2.896 ± 0.759
0.0ValXaa: 0.0 ± 0.0
Trp
0.263TrpAla: 0.263 ± 0.305
0.263TrpCys: 0.263 ± 0.29
1.58TrpAsp: 1.58 ± 0.678
1.053TrpGlu: 1.053 ± 0.471
0.527TrpPhe: 0.527 ± 0.388
0.79TrpGly: 0.79 ± 0.448
0.263TrpHis: 0.263 ± 0.296
0.527TrpIle: 0.527 ± 0.358
0.527TrpLys: 0.527 ± 0.369
1.316TrpLeu: 1.316 ± 0.732
0.0TrpMet: 0.0 ± 0.0
0.79TrpAsn: 0.79 ± 0.612
0.0TrpPro: 0.0 ± 0.0
0.79TrpGln: 0.79 ± 0.333
0.263TrpArg: 0.263 ± 0.252
0.79TrpSer: 0.79 ± 0.381
0.79TrpThr: 0.79 ± 0.427
0.263TrpVal: 0.263 ± 0.299
0.263TrpTrp: 0.263 ± 0.2
0.527TrpTyr: 0.527 ± 0.33
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.58TyrAla: 1.58 ± 0.599
0.0TyrCys: 0.0 ± 0.0
3.16TyrAsp: 3.16 ± 1.061
3.16TyrGlu: 3.16 ± 0.934
3.686TyrPhe: 3.686 ± 0.825
2.106TyrGly: 2.106 ± 0.809
0.79TyrHis: 0.79 ± 0.345
3.423TyrIle: 3.423 ± 0.655
5.793TyrLys: 5.793 ± 1.053
5.003TyrLeu: 5.003 ± 0.957
1.053TyrMet: 1.053 ± 0.586
2.896TyrAsn: 2.896 ± 0.662
0.527TyrPro: 0.527 ± 0.392
1.58TyrGln: 1.58 ± 0.746
1.843TyrArg: 1.843 ± 0.542
2.896TyrSer: 2.896 ± 1.084
1.58TyrThr: 1.58 ± 0.624
1.053TyrVal: 1.053 ± 0.511
0.79TyrTrp: 0.79 ± 0.39
1.053TyrTyr: 1.053 ± 0.441
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 27 proteins (3799 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski