Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2615 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q3AAL9|Q3AAL9_CARHZ Uncharacterized protein OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=CHY_1996 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 6.48 DD3 pKa = 2.83 IVGRR7 pKa = 11.84 VSYY10 pKa = 10.43 LQGLAEE16 pKa = 5.01 GINLKK21 pKa = 10.55 EE22 pKa = 4.02 KK23 pKa = 10.46 GKK25 pKa = 9.86 EE26 pKa = 3.96 GEE28 pKa = 4.6 IITEE32 pKa = 4.44 IIDD35 pKa = 4.63 LIADD39 pKa = 3.73 LTEE42 pKa = 4.24 EE43 pKa = 4.2 VRR45 pKa = 11.84 SLRR48 pKa = 11.84 DD49 pKa = 3.29 DD50 pKa = 3.51 QEE52 pKa = 4.24 RR53 pKa = 11.84 LEE55 pKa = 4.63 EE56 pKa = 4.06 YY57 pKa = 11.02 VEE59 pKa = 4.29 TVDD62 pKa = 4.71 DD63 pKa = 5.15 DD64 pKa = 4.98 LYY66 pKa = 10.69 EE67 pKa = 5.88 LEE69 pKa = 4.68 DD70 pKa = 3.63 AVYY73 pKa = 11.01 DD74 pKa = 4.0 DD75 pKa = 6.0 DD76 pKa = 5.98 EE77 pKa = 4.75 EE78 pKa = 4.39 FTYY81 pKa = 8.33 VTCPEE86 pKa = 4.29 CGEE89 pKa = 3.77 RR90 pKa = 11.84 VYY92 pKa = 10.88 FEE94 pKa = 5.22 RR95 pKa = 11.84 EE96 pKa = 3.75 LLEE99 pKa = 6.29 ADD101 pKa = 3.69 DD102 pKa = 5.37 NYY104 pKa = 11.01 EE105 pKa = 3.96 ITCPNCGALVLVSEE119 pKa = 5.34 FIEE122 pKa = 4.1 NRR124 pKa = 11.84 RR125 pKa = 11.84 EE126 pKa = 3.75 QALNQQNYY134 pKa = 10.42 DD135 pKa = 3.65 DD136 pKa = 4.79 VII138 pKa = 5.67
Molecular weight: 16.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.528
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.554
Grimsley 3.439
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 3.973
Thurlkill 3.567
EMBOSS 3.605
Sillero 3.834
Patrickios 1.825
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|Q3A8P8|Q3A8P8_CARHZ DNA gyrase subunit A OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=gyrA PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.61 QPKK8 pKa = 8.03 NRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 9.16 RR14 pKa = 11.84 VHH16 pKa = 6.3 GFLKK20 pKa = 10.47 RR21 pKa = 11.84 MRR23 pKa = 11.84 TPGGRR28 pKa = 11.84 NVIKK32 pKa = 10.48 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.22 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.199
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.457
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.179
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2615
0
2615
720219
28
1407
275.4
30.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.645 ± 0.05
0.899 ± 0.024
4.257 ± 0.031
7.945 ± 0.055
4.585 ± 0.041
7.524 ± 0.044
1.507 ± 0.02
7.488 ± 0.04
7.845 ± 0.043
10.995 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.113 ± 0.02
4.109 ± 0.034
4.192 ± 0.031
2.817 ± 0.027
4.704 ± 0.042
4.579 ± 0.028
4.808 ± 0.034
7.494 ± 0.042
0.851 ± 0.017
3.643 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here