Aspergillus niger (strain CBS 513.88 / FGSC A1513)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati; Aspergillus niger

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14068 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A2QH82|A2QH82_ASPNC Aspergillus niger contig An03c0180 genomic contig OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=An03g05930 PE=4 SV=1
MM1 pKa = 7.59AFKK4 pKa = 8.7TTAGLAFLALAGSVKK19 pKa = 10.35AQSVDD24 pKa = 2.83GSKK27 pKa = 11.16YY28 pKa = 9.83NSPSNGPPASYY39 pKa = 9.75FAAATTLPVAALQSAAAKK57 pKa = 10.14ASSVPSKK64 pKa = 10.16ATYY67 pKa = 9.19PVNTDD72 pKa = 3.23DD73 pKa = 6.32DD74 pKa = 4.58SPKK77 pKa = 10.06STIHH81 pKa = 6.41SDD83 pKa = 2.72WVKK86 pKa = 10.45FNQGAALSWVADD98 pKa = 3.6MDD100 pKa = 4.13VDD102 pKa = 5.35CDD104 pKa = 4.89GIDD107 pKa = 3.7YY108 pKa = 10.19KK109 pKa = 11.38CKK111 pKa = 10.76GNGDD115 pKa = 4.15GLPEE119 pKa = 4.35TNWGALSAYY128 pKa = 6.81EE129 pKa = 4.22VPWIVIPDD137 pKa = 3.55QFLTANEE144 pKa = 4.25DD145 pKa = 3.83LLPGNNVAAVICNGKK160 pKa = 8.75MYY162 pKa = 10.7YY163 pKa = 10.53GILGDD168 pKa = 4.46SNGDD172 pKa = 3.56DD173 pKa = 3.61PEE175 pKa = 4.56VTGEE179 pKa = 4.26ASWLMARR186 pKa = 11.84TCFPDD191 pKa = 4.84DD192 pKa = 4.5DD193 pKa = 5.37LNGAEE198 pKa = 4.32GHH200 pKa = 6.72AEE202 pKa = 3.82ADD204 pKa = 4.28VTCKK208 pKa = 10.36PYY210 pKa = 10.24TRR212 pKa = 11.84SALNKK217 pKa = 10.09NYY219 pKa = 8.33ITNFSTLRR227 pKa = 11.84SMGDD231 pKa = 3.28KK232 pKa = 10.77LVGALASNLGLTSSASGSTATCSWQGHH259 pKa = 5.4CEE261 pKa = 3.74GAICSTEE268 pKa = 4.19DD269 pKa = 3.48DD270 pKa = 4.32CSDD273 pKa = 5.38DD274 pKa = 4.41LVCDD278 pKa = 3.72SGKK281 pKa = 10.45CSSPDD286 pKa = 3.29EE287 pKa = 5.13DD288 pKa = 6.05DD289 pKa = 6.01GDD291 pKa = 5.31DD292 pKa = 4.57EE293 pKa = 6.49DD294 pKa = 6.8DD295 pKa = 4.03EE296 pKa = 6.29DD297 pKa = 5.26EE298 pKa = 4.72EE299 pKa = 5.4EE300 pKa = 4.42NDD302 pKa = 5.03EE303 pKa = 5.64DD304 pKa = 6.44DD305 pKa = 5.6EE306 pKa = 7.61DD307 pKa = 6.7DD308 pKa = 5.74DD309 pKa = 7.19DD310 pKa = 6.53EE311 pKa = 7.71DD312 pKa = 6.68DD313 pKa = 5.05EE314 pKa = 7.16DD315 pKa = 6.26DD316 pKa = 3.82EE317 pKa = 4.83

Molecular weight: 33.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A2RA46|A2RA46_ASPNC Aspergillus niger contig An18c0050 genomic contig OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=An18g01890 PE=4 SV=1
MM1 pKa = 7.04VNRR4 pKa = 11.84RR5 pKa = 11.84LRR7 pKa = 11.84VRR9 pKa = 11.84IRR11 pKa = 11.84ISGRR15 pKa = 11.84AVLRR19 pKa = 11.84TNIFNKK25 pKa = 9.78VV26 pKa = 2.87

Molecular weight: 3.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14068

0

14068

6183668

10

7035

439.6

48.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.363 ± 0.018

1.411 ± 0.009

5.506 ± 0.014

5.987 ± 0.024

3.656 ± 0.012

6.859 ± 0.018

2.513 ± 0.01

4.985 ± 0.015

4.386 ± 0.02

9.155 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.008

3.571 ± 0.01

6.069 ± 0.021

4.011 ± 0.014

6.259 ± 0.021

8.391 ± 0.023

6.017 ± 0.015

6.208 ± 0.015

1.524 ± 0.008

2.916 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski