Amino acid dipepetide frequency for Aspergillus niger (strain CBS 513.88 / FGSC A1513)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.538AlaAla: 8.538 ± 0.058	1.216AlaCys: 1.216 ± 0.016	4.116AlaAsp: 4.116 ± 0.025	4.885AlaGlu: 4.885 ± 0.035	3.074AlaPhe: 3.074 ± 0.024	5.733AlaGly: 5.733 ± 0.037	1.813AlaHis: 1.813 ± 0.019	4.272AlaIle: 4.272 ± 0.028	3.61AlaLys: 3.61 ± 0.027	7.661AlaLeu: 7.661 ± 0.045	1.982AlaMet: 1.982 ± 0.02	2.839AlaAsn: 2.839 ± 0.023	4.525AlaPro: 4.525 ± 0.04	3.255AlaGln: 3.255 ± 0.028	4.913AlaArg: 4.913 ± 0.034	7.2AlaSer: 7.2 ± 0.041	5.193AlaThr: 5.193 ± 0.033	5.396AlaVal: 5.396 ± 0.029	1.196AlaTrp: 1.196 ± 0.016	2.213AlaTyr: 2.213 ± 0.022	0.002AlaXaa: 0.002 ± 0.001
Cys	1.033CysAla: 1.033 ± 0.014	0.311CysCys: 0.311 ± 0.008	0.739CysAsp: 0.739 ± 0.012	0.645CysGlu: 0.645 ± 0.009	0.59CysPhe: 0.59 ± 0.01	1.024CysGly: 1.024 ± 0.015	0.422CysHis: 0.422 ± 0.008	0.795CysIle: 0.795 ± 0.012	0.508CysLys: 0.508 ± 0.011	1.523CysLeu: 1.523 ± 0.018	0.328CysMet: 0.328 ± 0.007	0.468CysAsn: 0.468 ± 0.009	0.764CysPro: 0.764 ± 0.014	0.541CysGln: 0.541 ± 0.01	0.942CysArg: 0.942 ± 0.012	1.126CysSer: 1.126 ± 0.016	0.789CysThr: 0.789 ± 0.012	0.901CysVal: 0.901 ± 0.012	0.255CysTrp: 0.255 ± 0.007	0.405CysTyr: 0.405 ± 0.008	0.001CysXaa: 0.001 ± 0.0
Asp	4.429AspAla: 4.429 ± 0.027	0.693AspCys: 0.693 ± 0.011	3.807AspAsp: 3.807 ± 0.043	4.058AspGlu: 4.058 ± 0.028	2.033AspPhe: 2.033 ± 0.019	3.896AspGly: 3.896 ± 0.028	1.282AspHis: 1.282 ± 0.015	3.068AspIle: 3.068 ± 0.022	2.169AspLys: 2.169 ± 0.019	5.038AspLeu: 5.038 ± 0.033	1.251AspMet: 1.251 ± 0.014	1.849AspAsn: 1.849 ± 0.018	3.33AspPro: 3.33 ± 0.025	1.866AspGln: 1.866 ± 0.018	3.157AspArg: 3.157 ± 0.027	3.997AspSer: 3.997 ± 0.033	2.961AspThr: 2.961 ± 0.024	3.571AspVal: 3.571 ± 0.02	0.912AspTrp: 0.912 ± 0.012	1.689AspTyr: 1.689 ± 0.017	0.001AspXaa: 0.001 ± 0.001
Glu	5.094GluAla: 5.094 ± 0.036	0.685GluCys: 0.685 ± 0.012	3.884GluAsp: 3.884 ± 0.03	5.256GluGlu: 5.256 ± 0.049	1.842GluPhe: 1.842 ± 0.016	3.807GluGly: 3.807 ± 0.027	1.376GluHis: 1.376 ± 0.014	2.92GluIle: 2.92 ± 0.025	3.358GluLys: 3.358 ± 0.033	5.027GluLeu: 5.027 ± 0.037	1.438GluMet: 1.438 ± 0.014	2.161GluAsn: 2.161 ± 0.022	2.783GluPro: 2.783 ± 0.035	2.464GluGln: 2.464 ± 0.023	3.919GluArg: 3.919 ± 0.032	4.267GluSer: 4.267 ± 0.03	3.451GluThr: 3.451 ± 0.026	3.519GluVal: 3.519 ± 0.026	0.895GluTrp: 0.895 ± 0.012	1.721GluTyr: 1.721 ± 0.019	0.001GluXaa: 0.001 ± 0.001
Phe	2.892PheAla: 2.892 ± 0.023	0.615PheCys: 0.615 ± 0.011	2.158PheAsp: 2.158 ± 0.02	1.982PheGlu: 1.982 ± 0.019	1.601PhePhe: 1.601 ± 0.021	2.725PheGly: 2.725 ± 0.03	0.97PheHis: 0.97 ± 0.014	1.818PheIle: 1.818 ± 0.019	1.299PheLys: 1.299 ± 0.015	3.59PheLeu: 3.59 ± 0.027	0.767PheMet: 0.767 ± 0.012	1.339PheAsn: 1.339 ± 0.017	2.072PhePro: 2.072 ± 0.02	1.389PheGln: 1.389 ± 0.015	2.07PheArg: 2.07 ± 0.019	3.023PheSer: 3.023 ± 0.022	2.135PheThr: 2.135 ± 0.019	2.332PheVal: 2.332 ± 0.023	0.647PheTrp: 0.647 ± 0.009	1.138PheTyr: 1.138 ± 0.014	0.001PheXaa: 0.001 ± 0.0
Gly	5.148GlyAla: 5.148 ± 0.035	1.039GlyCys: 1.039 ± 0.012	3.551GlyAsp: 3.551 ± 0.023	3.624GlyGlu: 3.624 ± 0.027	2.742GlyPhe: 2.742 ± 0.025	5.575GlyGly: 5.575 ± 0.053	1.769GlyHis: 1.769 ± 0.018	3.597GlyIle: 3.597 ± 0.024	3.264GlyLys: 3.264 ± 0.027	6.215GlyLeu: 6.215 ± 0.036	1.656GlyMet: 1.656 ± 0.016	2.438GlyAsn: 2.438 ± 0.023	3.359GlyPro: 3.359 ± 0.029	2.567GlyGln: 2.567 ± 0.025	4.317GlyArg: 4.317 ± 0.028	5.756GlySer: 5.756 ± 0.038	3.968GlyThr: 3.968 ± 0.03	4.516GlyVal: 4.516 ± 0.031	1.259GlyTrp: 1.259 ± 0.016	2.232GlyTyr: 2.232 ± 0.026	0.002GlyXaa: 0.002 ± 0.001
His	1.946HisAla: 1.946 ± 0.019	0.407HisCys: 0.407 ± 0.008	1.337HisAsp: 1.337 ± 0.017	1.332HisGlu: 1.332 ± 0.014	0.92HisPhe: 0.92 ± 0.01	1.781HisGly: 1.781 ± 0.017	0.97HisHis: 0.97 ± 0.017	1.281HisIle: 1.281 ± 0.014	0.843HisLys: 0.843 ± 0.011	2.424HisLeu: 2.424 ± 0.017	0.527HisMet: 0.527 ± 0.01	0.877HisAsn: 0.877 ± 0.011	1.873HisPro: 1.873 ± 0.019	1.014HisGln: 1.014 ± 0.013	1.641HisArg: 1.641 ± 0.019	1.956HisSer: 1.956 ± 0.018	1.402HisThr: 1.402 ± 0.018	1.462HisVal: 1.462 ± 0.016	0.376HisTrp: 0.376 ± 0.007	0.757HisTyr: 0.757 ± 0.011	0.001HisXaa: 0.001 ± 0.0
Ile	4.138IleAla: 4.138 ± 0.03	0.87IleCys: 0.87 ± 0.012	2.795IleAsp: 2.795 ± 0.02	2.744IleGlu: 2.744 ± 0.023	2.004IlePhe: 2.004 ± 0.019	3.253IleGly: 3.253 ± 0.024	1.328IleHis: 1.328 ± 0.014	2.596IleIle: 2.596 ± 0.024	1.968IleLys: 1.968 ± 0.021	4.802IleLeu: 4.802 ± 0.031	1.053IleMet: 1.053 ± 0.012	1.771IleAsn: 1.771 ± 0.018	3.244IlePro: 3.244 ± 0.027	1.954IleGln: 1.954 ± 0.021	2.953IleArg: 2.953 ± 0.024	4.023IleSer: 4.023 ± 0.024	2.888IleThr: 2.888 ± 0.021	3.187IleVal: 3.187 ± 0.023	0.741IleTrp: 0.741 ± 0.012	1.537IleTyr: 1.537 ± 0.017	0.003IleXaa: 0.003 ± 0.001
Lys	3.787LysAla: 3.787 ± 0.03	0.515LysCys: 0.515 ± 0.01	2.457LysAsp: 2.457 ± 0.025	3.107LysGlu: 3.107 ± 0.029	1.266LysPhe: 1.266 ± 0.015	2.8LysGly: 2.8 ± 0.022	1.063LysHis: 1.063 ± 0.015	2.098LysIle: 2.098 ± 0.018	2.789LysLys: 2.789 ± 0.033	3.746LysLeu: 3.746 ± 0.027	0.917LysMet: 0.917 ± 0.012	1.563LysAsn: 1.563 ± 0.016	2.523LysPro: 2.523 ± 0.025	1.76LysGln: 1.76 ± 0.019	3.187LysArg: 3.187 ± 0.027	3.195LysSer: 3.195 ± 0.025	2.494LysThr: 2.494 ± 0.025	2.624LysVal: 2.624 ± 0.025	0.637LysTrp: 0.637 ± 0.009	1.33LysTyr: 1.33 ± 0.017	0.001LysXaa: 0.001 ± 0.001
Leu	7.824LeuAla: 7.824 ± 0.04	1.399LeuCys: 1.399 ± 0.017	5.13LeuAsp: 5.13 ± 0.029	5.391LeuGlu: 5.391 ± 0.035	3.452LeuPhe: 3.452 ± 0.027	6.128LeuGly: 6.128 ± 0.032	2.394LeuHis: 2.394 ± 0.021	4.155LeuIle: 4.155 ± 0.032	3.854LeuLys: 3.854 ± 0.031	8.961LeuLeu: 8.961 ± 0.051	1.863LeuMet: 1.863 ± 0.015	3.156LeuAsn: 3.156 ± 0.023	5.705LeuPro: 5.705 ± 0.033	3.932LeuGln: 3.932 ± 0.029	5.973LeuArg: 5.973 ± 0.038	7.768LeuSer: 7.768 ± 0.044	5.038LeuThr: 5.038 ± 0.032	5.609LeuVal: 5.609 ± 0.033	1.263LeuTrp: 1.263 ± 0.017	2.552LeuTyr: 2.552 ± 0.021	0.004LeuXaa: 0.004 ± 0.001
Met	2.178MetAla: 2.178 ± 0.022	0.267MetCys: 0.267 ± 0.007	1.245MetAsp: 1.245 ± 0.015	1.324MetGlu: 1.324 ± 0.015	0.741MetPhe: 0.741 ± 0.01	1.546MetGly: 1.546 ± 0.017	0.519MetHis: 0.519 ± 0.009	1.06MetIle: 1.06 ± 0.012	0.959MetLys: 0.959 ± 0.012	1.904MetLeu: 1.904 ± 0.019	0.579MetMet: 0.579 ± 0.01	0.813MetAsn: 0.813 ± 0.011	1.278MetPro: 1.278 ± 0.015	0.879MetGln: 0.879 ± 0.011	1.329MetArg: 1.329 ± 0.015	1.909MetSer: 1.909 ± 0.02	1.357MetThr: 1.357 ± 0.016	1.376MetVal: 1.376 ± 0.015	0.273MetTrp: 0.273 ± 0.006	0.55MetTyr: 0.55 ± 0.009	0.001MetXaa: 0.001 ± 0.001
Asn	2.967AsnAla: 2.967 ± 0.023	0.494AsnCys: 0.494 ± 0.009	1.828AsnAsp: 1.828 ± 0.019	1.937AsnGlu: 1.937 ± 0.018	1.271AsnPhe: 1.271 ± 0.017	2.821AsnGly: 2.821 ± 0.028	0.901AsnHis: 0.901 ± 0.012	2.025AsnIle: 2.025 ± 0.018	1.421AsnLys: 1.421 ± 0.017	3.186AsnLeu: 3.186 ± 0.026	0.838AsnMet: 0.838 ± 0.012	1.502AsnAsn: 1.502 ± 0.023	2.453AsnPro: 2.453 ± 0.021	1.318AsnGln: 1.318 ± 0.017	1.984AsnArg: 1.984 ± 0.015	2.665AsnSer: 2.665 ± 0.023	2.15AsnThr: 2.15 ± 0.021	2.271AsnVal: 2.271 ± 0.021	0.572AsnTrp: 0.572 ± 0.011	1.11AsnTyr: 1.11 ± 0.015	0.001AsnXaa: 0.001 ± 0.001
Pro	5.156ProAla: 5.156 ± 0.04	0.652ProCys: 0.652 ± 0.012	3.236ProAsp: 3.236 ± 0.022	3.841ProGlu: 3.841 ± 0.035	2.118ProPhe: 2.118 ± 0.019	3.957ProGly: 3.957 ± 0.029	1.418ProHis: 1.418 ± 0.019	2.643ProIle: 2.643 ± 0.022	2.404ProLys: 2.404 ± 0.022	4.903ProLeu: 4.903 ± 0.038	1.125ProMet: 1.125 ± 0.017	2.146ProAsn: 2.146 ± 0.023	4.788ProPro: 4.788 ± 0.062	2.444ProGln: 2.444 ± 0.026	3.508ProArg: 3.508 ± 0.027	6.182ProSer: 6.182 ± 0.044	4.027ProThr: 4.027 ± 0.028	3.736ProVal: 3.736 ± 0.03	0.825ProTrp: 0.825 ± 0.011	1.58ProTyr: 1.58 ± 0.017	0.003ProXaa: 0.003 ± 0.001
Gln	3.369GlnAla: 3.369 ± 0.027	0.517GlnCys: 0.517 ± 0.01	1.994GlnAsp: 1.994 ± 0.018	2.388GlnGlu: 2.388 ± 0.022	1.294GlnPhe: 1.294 ± 0.017	2.436GlnGly: 2.436 ± 0.018	1.083GlnHis: 1.083 ± 0.015	1.911GlnIle: 1.911 ± 0.02	1.878GlnLys: 1.878 ± 0.018	3.621GlnLeu: 3.621 ± 0.029	0.886GlnMet: 0.886 ± 0.012	1.469GlnAsn: 1.469 ± 0.017	2.589GlnPro: 2.589 ± 0.032	2.403GlnGln: 2.403 ± 0.04	2.644GlnArg: 2.644 ± 0.021	3.256GlnSer: 3.256 ± 0.026	2.346GlnThr: 2.346 ± 0.02	2.216GlnVal: 2.216 ± 0.022	0.609GlnTrp: 0.609 ± 0.01	1.204GlnTyr: 1.204 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.731ArgAla: 4.731 ± 0.028	0.883ArgCys: 0.883 ± 0.012	3.342ArgAsp: 3.342 ± 0.027	3.837ArgGlu: 3.837 ± 0.028	2.181ArgPhe: 2.181 ± 0.018	3.867ArgGly: 3.867 ± 0.029	1.63ArgHis: 1.63 ± 0.019	2.984ArgIle: 2.984 ± 0.021	3.314ArgLys: 3.314 ± 0.026	5.859ArgLeu: 5.859 ± 0.042	1.401ArgMet: 1.401 ± 0.018	2.229ArgAsn: 2.229 ± 0.02	3.608ArgPro: 3.608 ± 0.03	2.723ArgGln: 2.723 ± 0.023	5.194ArgArg: 5.194 ± 0.044	5.016ArgSer: 5.016 ± 0.035	3.359ArgThr: 3.359 ± 0.022	3.601ArgVal: 3.601 ± 0.024	0.998ArgTrp: 0.998 ± 0.016	1.831ArgTyr: 1.831 ± 0.021	0.002ArgXaa: 0.002 ± 0.001
Ser	6.654SerAla: 6.654 ± 0.036	1.056SerCys: 1.056 ± 0.015	4.256SerAsp: 4.256 ± 0.032	4.156SerGlu: 4.156 ± 0.025	3.074SerPhe: 3.074 ± 0.024	5.65SerGly: 5.65 ± 0.043	2.103SerHis: 2.103 ± 0.02	4.108SerIle: 4.108 ± 0.031	3.345SerLys: 3.345 ± 0.027	7.653SerLeu: 7.653 ± 0.04	1.78SerMet: 1.78 ± 0.018	2.944SerAsn: 2.944 ± 0.022	5.509SerPro: 5.509 ± 0.041	3.359SerGln: 3.359 ± 0.026	5.224SerArg: 5.224 ± 0.032	9.057SerSer: 9.057 ± 0.062	5.751SerThr: 5.751 ± 0.042	4.823SerVal: 4.823 ± 0.035	1.21SerTrp: 1.21 ± 0.014	2.197SerTyr: 2.197 ± 0.022	0.002SerXaa: 0.002 ± 0.001
Thr	5.163ThrAla: 5.163 ± 0.033	0.834ThrCys: 0.834 ± 0.011	2.95ThrAsp: 2.95 ± 0.021	3.152ThrGlu: 3.152 ± 0.025	2.152ThrPhe: 2.152 ± 0.019	4.317ThrGly: 4.317 ± 0.028	1.38ThrHis: 1.38 ± 0.017	3.135ThrIle: 3.135 ± 0.025	2.387ThrLys: 2.387 ± 0.021	5.38ThrLeu: 5.38 ± 0.031	1.236ThrMet: 1.236 ± 0.015	2.119ThrAsn: 2.119 ± 0.02	4.234ThrPro: 4.234 ± 0.031	2.081ThrGln: 2.081 ± 0.019	3.209ThrArg: 3.209 ± 0.021	5.353ThrSer: 5.353 ± 0.042	4.557ThrThr: 4.557 ± 0.044	3.905ThrVal: 3.905 ± 0.026	0.875ThrTrp: 0.875 ± 0.012	1.742ThrTyr: 1.742 ± 0.021	0.003ThrXaa: 0.003 ± 0.001
Val	5.192ValAla: 5.192 ± 0.033	0.946ValCys: 0.946 ± 0.013	3.71ValAsp: 3.71 ± 0.024	3.776ValGlu: 3.776 ± 0.031	2.426ValPhe: 2.426 ± 0.023	4.101ValGly: 4.101 ± 0.03	1.487ValHis: 1.487 ± 0.017	3.069ValIle: 3.069 ± 0.024	2.652ValLys: 2.652 ± 0.025	5.715ValLeu: 5.715 ± 0.031	1.379ValMet: 1.379 ± 0.015	2.237ValAsn: 2.237 ± 0.023	3.708ValPro: 3.708 ± 0.027	2.431ValGln: 2.431 ± 0.022	3.641ValArg: 3.641 ± 0.026	4.867ValSer: 4.867 ± 0.03	3.593ValThr: 3.593 ± 0.027	4.364ValVal: 4.364 ± 0.031	0.916ValTrp: 0.916 ± 0.012	1.865ValTyr: 1.865 ± 0.018	0.001ValXaa: 0.001 ± 0.001
Trp	1.162TrpAla: 1.162 ± 0.014	0.222TrpCys: 0.222 ± 0.006	0.894TrpAsp: 0.894 ± 0.012	0.862TrpGlu: 0.862 ± 0.012	0.556TrpPhe: 0.556 ± 0.01	0.995TrpGly: 0.995 ± 0.013	0.376TrpHis: 0.376 ± 0.008	0.806TrpIle: 0.806 ± 0.012	0.81TrpLys: 0.81 ± 0.011	1.462TrpLeu: 1.462 ± 0.016	0.402TrpMet: 0.402 ± 0.008	0.637TrpAsn: 0.637 ± 0.012	0.675TrpPro: 0.675 ± 0.011	0.58TrpGln: 0.58 ± 0.009	1.011TrpArg: 1.011 ± 0.013	1.113TrpSer: 1.113 ± 0.013	0.971TrpThr: 0.971 ± 0.014	0.96TrpVal: 0.96 ± 0.013	0.297TrpTrp: 0.297 ± 0.007	0.452TrpTyr: 0.452 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.23TyrAla: 2.23 ± 0.02	0.49TyrCys: 0.49 ± 0.01	1.625TyrAsp: 1.625 ± 0.018	1.569TyrGlu: 1.569 ± 0.016	1.224TyrPhe: 1.224 ± 0.015	2.181TyrGly: 2.181 ± 0.023	0.842TyrHis: 0.842 ± 0.011	1.508TyrIle: 1.508 ± 0.019	1.026TyrLys: 1.026 ± 0.014	2.879TyrLeu: 2.879 ± 0.021	0.681TyrMet: 0.681 ± 0.012	1.156TyrAsn: 1.156 ± 0.015	1.673TyrPro: 1.673 ± 0.022	1.153TyrGln: 1.153 ± 0.015	1.766TyrArg: 1.766 ± 0.016	2.18TyrSer: 2.18 ± 0.021	1.733TyrThr: 1.733 ± 0.02	1.703TyrVal: 1.703 ± 0.018	0.488TyrTrp: 0.488 ± 0.01	1.054TyrTyr: 1.054 ± 0.017	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.003XaaLys: 0.003 ± 0.001	0.003XaaLeu: 0.003 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.001XaaXaa: 0.001 ± 0.0

Statistics based on 14068 proteins (6183668 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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