Stylophora pistillata (Smooth cauliflower coral)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24067 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2B4SQ96|A0A2B4SQ96_STYPI Protein notum-like OS=Stylophora pistillata OX=50429 GN=Notum PE=3 SV=1
MM1 pKa = 7.16 EE2 pKa = 4.59 TFSNRR7 pKa = 11.84 EE8 pKa = 3.93 LIEE11 pKa = 3.96 ASKK14 pKa = 10.57 MMNFVDD20 pKa = 5.39 EE21 pKa = 4.51 VPGWGSPYY29 pKa = 10.14 CVEE32 pKa = 4.61 GLPFLDD38 pKa = 4.05 GTQVVPEE45 pKa = 4.24 MTAQDD50 pKa = 4.0 LDD52 pKa = 4.41 GYY54 pKa = 10.32 PPMQLDD60 pKa = 3.92 GEE62 pKa = 4.44 EE63 pKa = 4.27 SKK65 pKa = 11.06 SRR67 pKa = 11.84 SDD69 pKa = 5.41 GVTSWKK75 pKa = 10.24 SCSSLTDD82 pKa = 3.36 HH83 pKa = 6.96 EE84 pKa = 4.91 SGYY87 pKa = 11.22 SSDD90 pKa = 3.81 EE91 pKa = 4.2 SASPRR96 pKa = 11.84 MDD98 pKa = 3.23 EE99 pKa = 5.03 ALPSPANNCLDD110 pKa = 4.29 LDD112 pKa = 4.07 QNTTPGLMEE121 pKa = 4.53 CLGPTEE127 pKa = 5.25 DD128 pKa = 3.43 WFNYY132 pKa = 8.98 PPYY135 pKa = 9.56 TWTAAQLEE143 pKa = 4.66 SFLKK147 pKa = 10.33 SVRR150 pKa = 11.84 EE151 pKa = 4.15 SYY153 pKa = 10.71 NIPGEE158 pKa = 4.2 CDD160 pKa = 3.41 LEE162 pKa = 4.81 KK163 pKa = 10.92 LDD165 pKa = 4.48 LTGPEE170 pKa = 4.62 LWCSTADD177 pKa = 3.47 DD178 pKa = 4.09 LKK180 pKa = 11.16 RR181 pKa = 11.84 RR182 pKa = 11.84 TEE184 pKa = 3.98 YY185 pKa = 11.55 GEE187 pKa = 4.23 LLYY190 pKa = 10.62 EE191 pKa = 4.8 ALNQFPSNSQWEE203 pKa = 4.7 SNSTSCGGYY212 pKa = 9.66 KK213 pKa = 10.38 DD214 pKa = 4.12 PSSDD218 pKa = 3.99 EE219 pKa = 4.02 MFNLQILAEE228 pKa = 4.2 QDD230 pKa = 3.52 PSSSPFLDD238 pKa = 3.96 DD239 pKa = 5.3 LPVEE243 pKa = 4.79 GKK245 pKa = 8.01 TLSHH249 pKa = 6.18 LTIPQIILL257 pKa = 3.3
Molecular weight: 28.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.668
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.732
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.126
Thurlkill 3.706
EMBOSS 3.745
Sillero 3.986
Patrickios 1.214
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A2B4S848|A0A2B4S848_STYPI Uncharacterized protein OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene10595 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 10.53 VAGKK6 pKa = 9.52 SQLSRR11 pKa = 11.84 KK12 pKa = 9.11 KK13 pKa = 10.18 VARR16 pKa = 11.84 KK17 pKa = 9.05 SQLSRR22 pKa = 11.84 KK23 pKa = 8.89 KK24 pKa = 10.18 VARR27 pKa = 11.84 KK28 pKa = 9.05 SQLSRR33 pKa = 11.84 KK34 pKa = 8.89 KK35 pKa = 10.18 VARR38 pKa = 11.84 KK39 pKa = 9.05 SQLSRR44 pKa = 11.84 KK45 pKa = 8.89 KK46 pKa = 10.18 VARR49 pKa = 11.84 KK50 pKa = 9.05 SQLSRR55 pKa = 11.84 KK56 pKa = 8.89 KK57 pKa = 10.14 VARR60 pKa = 11.84 KK61 pKa = 8.62 SQPSRR66 pKa = 11.84 KK67 pKa = 9.07 KK68 pKa = 9.99 VARR71 pKa = 11.84 KK72 pKa = 9.05 SQLSRR77 pKa = 11.84 KK78 pKa = 8.89 KK79 pKa = 10.18 VARR82 pKa = 11.84 KK83 pKa = 9.05 SQLSRR88 pKa = 11.84 KK89 pKa = 8.89 KK90 pKa = 10.18 VARR93 pKa = 11.84 KK94 pKa = 9.05 SQLSRR99 pKa = 11.84 KK100 pKa = 8.89 KK101 pKa = 10.18 VARR104 pKa = 11.84 KK105 pKa = 9.05 SQLSRR110 pKa = 11.84 KK111 pKa = 8.89 KK112 pKa = 10.18 VARR115 pKa = 11.84 KK116 pKa = 9.05 SQLSGKK122 pKa = 9.56 KK123 pKa = 8.19 VARR126 pKa = 11.84 KK127 pKa = 9.05 SQLSRR132 pKa = 11.84 KK133 pKa = 8.89 KK134 pKa = 10.18 VARR137 pKa = 11.84 KK138 pKa = 9.05 SQLSRR143 pKa = 11.84 KK144 pKa = 8.89 KK145 pKa = 10.18 VARR148 pKa = 11.84 KK149 pKa = 9.05 SQLSRR154 pKa = 11.84 KK155 pKa = 8.89 KK156 pKa = 10.18 VARR159 pKa = 11.84 KK160 pKa = 9.05 SQLSRR165 pKa = 11.84 KK166 pKa = 8.89 KK167 pKa = 10.18 VARR170 pKa = 11.84 KK171 pKa = 9.05 SQLSRR176 pKa = 11.84 KK177 pKa = 8.89 KK178 pKa = 10.18 VARR181 pKa = 11.84 KK182 pKa = 9.05 SQLSRR187 pKa = 11.84 KK188 pKa = 8.89 KK189 pKa = 10.18 VARR192 pKa = 11.84 KK193 pKa = 9.05 SQLSRR198 pKa = 11.84 KK199 pKa = 8.89 KK200 pKa = 10.18 VARR203 pKa = 11.84 KK204 pKa = 9.05 SQLSRR209 pKa = 11.84 KK210 pKa = 8.89 KK211 pKa = 10.18 VARR214 pKa = 11.84 KK215 pKa = 9.05 SQLSRR220 pKa = 11.84 KK221 pKa = 9.91 KK222 pKa = 10.11 EE223 pKa = 4.13 DD224 pKa = 3.49 CSKK227 pKa = 10.93 AAPP230 pKa = 3.71
Molecular weight: 26.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.228
IPC_protein 12.764
Toseland 12.954
ProMoST 13.437
Dawson 12.954
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 12.881
Grimsley 12.998
Solomon 13.437
Lehninger 13.334
Nozaki 12.954
DTASelect 12.939
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.588
IPC_peptide 13.437
IPC2_peptide 12.427
IPC2.peptide.svr19 9.109
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24067
0
24067
14677808
31
15904
609.9
68.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.135 ± 0.011
2.158 ± 0.016
5.635 ± 0.013
7.131 ± 0.024
3.986 ± 0.011
6.044 ± 0.022
2.357 ± 0.007
5.124 ± 0.011
6.955 ± 0.014
8.997 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.089 ± 0.006
4.648 ± 0.011
4.623 ± 0.018
4.097 ± 0.012
5.501 ± 0.015
8.19 ± 0.019
5.727 ± 0.012
6.494 ± 0.013
1.191 ± 0.005
2.916 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here