Streptococcus phage 109751

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F4W1|A0A3G8F4W1_9CAUD Uncharacterized protein OS=Streptococcus phage 109751 OX=2484225 GN=109751_00013 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.42SDD5 pKa = 3.89LGPYY9 pKa = 9.9LDD11 pKa = 4.98AVTNEE16 pKa = 4.42DD17 pKa = 3.65GTLLICKK24 pKa = 8.59TEE26 pKa = 3.77QGAYY30 pKa = 9.48IGDD33 pKa = 4.28FNPSCDD39 pKa = 3.67EE40 pKa = 3.84EE41 pKa = 5.78DD42 pKa = 3.78FVLTYY47 pKa = 11.02EE48 pKa = 4.44DD49 pKa = 3.86VSVSLSYY56 pKa = 11.13AQVLSATLLKK66 pKa = 10.84VV67 pKa = 3.17

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F519|A0A3G8F519_9CAUD Cro/CI family transcriptional regulator OS=Streptococcus phage 109751 OX=2484225 GN=109751_00009 PE=4 SV=1
MM1 pKa = 7.4SGVNVKK7 pKa = 9.77IDD9 pKa = 3.59LKK11 pKa = 10.93GVEE14 pKa = 4.71KK15 pKa = 10.54KK16 pKa = 10.69ASPEE20 pKa = 3.63NFAKK24 pKa = 10.69GKK26 pKa = 9.91LAIANQMLLDD36 pKa = 3.89MDD38 pKa = 4.71PYY40 pKa = 9.3VPKK43 pKa = 10.63RR44 pKa = 11.84KK45 pKa = 10.13GILRR49 pKa = 11.84ASGHH53 pKa = 4.84VRR55 pKa = 11.84QDD57 pKa = 2.84AVIYY61 pKa = 7.06VAPYY65 pKa = 10.76ARR67 pKa = 11.84LLYY70 pKa = 10.12YY71 pKa = 10.27GKK73 pKa = 9.95KK74 pKa = 9.48RR75 pKa = 11.84KK76 pKa = 9.93GFFSEE81 pKa = 4.06KK82 pKa = 8.54QRR84 pKa = 11.84KK85 pKa = 8.15FFFANKK91 pKa = 9.44EE92 pKa = 3.94KK93 pKa = 10.8LLSQKK98 pKa = 7.49PTPGTGPRR106 pKa = 11.84WDD108 pKa = 3.54KK109 pKa = 10.92KK110 pKa = 10.61AAALHH115 pKa = 5.28SKK117 pKa = 9.51KK118 pKa = 9.58WAEE121 pKa = 4.16VGAKK125 pKa = 10.44AMGVKK130 pKa = 10.02

Molecular weight: 14.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12100

43

1159

198.4

22.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.709

0.521 ± 0.083

6.298 ± 0.293

7.645 ± 0.464

4.0 ± 0.219

6.421 ± 0.546

1.529 ± 0.185

6.975 ± 0.266

8.868 ± 0.508

8.314 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.177

5.281 ± 0.248

2.86 ± 0.238

4.058 ± 0.181

4.157 ± 0.282

6.306 ± 0.36

5.628 ± 0.195

6.388 ± 0.268

1.165 ± 0.159

4.091 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski