Arundinibacter roseus
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4679 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R4K275|A0A4R4K275_9BACT Lrp/AsnC family transcriptional regulator OS=Arundinibacter roseus OX=2070510 GN=EZE20_19325 PE=4 SV=1
TT1 pKa = 5.8 YY2 pKa = 9.72 TPDD5 pKa = 3.56 PGFVGEE11 pKa = 6.19 DD12 pKa = 3.66 VFCYY16 pKa = 10.08 AASDD20 pKa = 4.98 GIASDD25 pKa = 3.96 TACVAVTVQPNPTPANDD42 pKa = 3.85 SPLALNDD49 pKa = 3.37 NTQTTEE55 pKa = 3.98 GTPLSVNVLANDD67 pKa = 3.42 VDD69 pKa = 4.18 PDD71 pKa = 3.69 GDD73 pKa = 3.77 ALQNPTVVQAPANGTASVNADD94 pKa = 3.01 GTILYY99 pKa = 8.88 TPDD102 pKa = 3.54 PGFVGTDD109 pKa = 2.9 TLTYY113 pKa = 9.67 RR114 pKa = 11.84 VCDD117 pKa = 3.17 TGTPSLCDD125 pKa = 3.24 SAQVIVQVLPAPVDD139 pKa = 3.44 PAANQAPIAVDD150 pKa = 3.54 DD151 pKa = 4.95 ANTTTVNTPVTGTVAANDD169 pKa = 3.84 SDD171 pKa = 4.5 PDD173 pKa = 3.7 GDD175 pKa = 4.13 ALTFAGLTNPANGTLTVNADD195 pKa = 2.93 GSYY198 pKa = 10.79 VYY200 pKa = 10.35 TPAADD205 pKa = 4.85 FIGSDD210 pKa = 3.14 VFTYY214 pKa = 7.31 TVCDD218 pKa = 3.23 NGTPSLCDD226 pKa = 3.0 TATVFITVTPVAGKK240 pKa = 10.03 VALLPKK246 pKa = 10.28 VYY248 pKa = 10.51 LQGALFGVFLPDD260 pKa = 2.78 TLMRR264 pKa = 11.84 DD265 pKa = 3.78 DD266 pKa = 5.76 LRR268 pKa = 11.84 LKK270 pKa = 10.81 NLIPTTSPYY279 pKa = 10.69 PDD281 pKa = 3.49 MGLIGITSANVANITVVNASTTSTNNSIVDD311 pKa = 3.44 WVFVEE316 pKa = 4.76 LRR318 pKa = 11.84 SALDD322 pKa = 3.24 STLVLDD328 pKa = 3.99 SRR330 pKa = 11.84 SALVQRR336 pKa = 11.84 DD337 pKa = 3.31 GDD339 pKa = 3.84 IVEE342 pKa = 4.22 VDD344 pKa = 3.63 GLSAITFDD352 pKa = 3.68 SAVPGSYY359 pKa = 9.94 YY360 pKa = 10.53 VVVRR364 pKa = 11.84 HH365 pKa = 6.09 RR366 pKa = 11.84 NHH368 pKa = 6.71 LGVMSASPIALSTATTVVDD387 pKa = 4.06 FRR389 pKa = 11.84 KK390 pKa = 10.07 AATPTFNLNAASAVNIPQVPVQQGVALWAGNALYY424 pKa = 10.92 LNTLDD429 pKa = 4.59 SMRR432 pKa = 11.84 EE433 pKa = 4.07 VIFQGPDD440 pKa = 2.65 NDD442 pKa = 3.73 VNVIYY447 pKa = 10.24 QQVINSPANALFKK460 pKa = 11.29 SPFFNLKK467 pKa = 9.89 GYY469 pKa = 10.86 YY470 pKa = 10.22 NGDD473 pKa = 2.73 INMNGEE479 pKa = 4.45 TIFQGTGNDD488 pKa = 3.45 VEE490 pKa = 5.56 FIYY493 pKa = 11.05 QNVQKK498 pKa = 10.44 NHH500 pKa = 6.27 EE501 pKa = 4.6 GNTLKK506 pKa = 10.74 QPFFKK511 pKa = 10.29 IKK513 pKa = 10.15 EE514 pKa = 4.03 QTPP517 pKa = 3.36
Molecular weight: 54.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.821
Rodwell 3.656
Grimsley 3.49
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.291
Thurlkill 3.656
EMBOSS 3.834
Sillero 3.961
Patrickios 1.341
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.841
Protein with the highest isoelectric point:
>tr|A0A4R4KG47|A0A4R4KG47_9BACT Efflux RND transporter periplasmic adaptor subunit OS=Arundinibacter roseus OX=2070510 GN=EZE20_08595 PE=3 SV=1
MM1 pKa = 7.61 SNLKK5 pKa = 10.65 NYY7 pKa = 8.47 FQLGDD12 pKa = 3.52 VFSYY16 pKa = 8.96 FIRR19 pKa = 11.84 VFKK22 pKa = 10.73 KK23 pKa = 10.28 PSSDD27 pKa = 3.39 APNSFSLRR35 pKa = 11.84 MMHH38 pKa = 7.15 GINRR42 pKa = 11.84 ISIVMFLFCVVVMIIRR58 pKa = 11.84 AIMRR62 pKa = 3.93
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.867
IPC_protein 10.657
Toseland 10.701
ProMoST 10.935
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.082
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.604
IPC2.peptide.svr19 8.539
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4679
0
4679
1707330
13
3951
364.9
40.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.645 ± 0.038
0.81 ± 0.013
5.164 ± 0.028
6.09 ± 0.04
4.898 ± 0.027
7.054 ± 0.041
1.88 ± 0.02
6.255 ± 0.029
5.675 ± 0.036
10.031 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.274 ± 0.016
4.768 ± 0.035
4.38 ± 0.026
4.155 ± 0.024
4.813 ± 0.033
6.427 ± 0.033
6.031 ± 0.042
6.501 ± 0.028
1.346 ± 0.014
3.802 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here