Gordonia phage Lambo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambovirus; Gordonia virus Lambo

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TS31|A0A5J6TS31_9CAUD DNA-directed DNA polymerase OS=Gordonia phage Lambo OX=2599845 GN=77 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.01FTRR5 pKa = 11.84CTEE8 pKa = 4.27CGGSCDD14 pKa = 3.87PVFDD18 pKa = 4.02MVTVPAAFGEE28 pKa = 4.56VEE30 pKa = 4.83IPMCCDD36 pKa = 3.12CYY38 pKa = 11.01EE39 pKa = 4.12EE40 pKa = 5.05VYY42 pKa = 11.17GEE44 pKa = 4.62AGSEE48 pKa = 4.13DD49 pKa = 3.54

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TT11|A0A5J6TT11_9CAUD SAP domain-containing protein OS=Gordonia phage Lambo OX=2599845 GN=16 PE=4 SV=1
MM1 pKa = 8.02PDD3 pKa = 3.07YY4 pKa = 11.21GSTPKK9 pKa = 10.2VPKK12 pKa = 9.89RR13 pKa = 11.84RR14 pKa = 11.84QRR16 pKa = 11.84PKK18 pKa = 9.52MRR20 pKa = 11.84QQRR23 pKa = 11.84RR24 pKa = 11.84DD25 pKa = 3.49YY26 pKa = 10.38KK27 pKa = 10.4RR28 pKa = 11.84KK29 pKa = 8.79RR30 pKa = 11.84DD31 pKa = 3.53RR32 pKa = 11.84EE33 pKa = 4.15GRR35 pKa = 11.84FASTDD40 pKa = 3.02RR41 pKa = 11.84SVSSRR46 pKa = 11.84RR47 pKa = 11.84YY48 pKa = 8.83IRR50 pKa = 11.84EE51 pKa = 3.68LRR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 9.13SKK57 pKa = 10.6NPRR60 pKa = 11.84RR61 pKa = 11.84TRR63 pKa = 11.84NTSKK67 pKa = 10.85NIYY70 pKa = 8.7NEE72 pKa = 3.89RR73 pKa = 11.84GGLKK77 pKa = 10.05SASAKK82 pKa = 9.88RR83 pKa = 11.84AGKK86 pKa = 10.18RR87 pKa = 11.84RR88 pKa = 11.84GTARR92 pKa = 11.84NNSKK96 pKa = 9.03RR97 pKa = 11.84TRR99 pKa = 11.84RR100 pKa = 3.57

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

21619

22

2402

220.6

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.399 ± 0.644

0.842 ± 0.12

6.624 ± 0.251

6.934 ± 0.315

3.372 ± 0.152

8.844 ± 0.357

1.892 ± 0.197

5.356 ± 0.213

5.939 ± 0.344

7.239 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.11

4.149 ± 0.202

4.885 ± 0.277

3.354 ± 0.172

6.106 ± 0.239

5.791 ± 0.215

5.648 ± 0.219

6.277 ± 0.189

1.841 ± 0.161

2.979 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski