Amino acid dipepetide frequency for Gordonia phage Lambo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.593AlaAla: 10.593 ± 1.717
0.555AlaCys: 0.555 ± 0.168
5.458AlaAsp: 5.458 ± 0.53
7.864AlaGlu: 7.864 ± 0.81
2.914AlaPhe: 2.914 ± 0.43
6.43AlaGly: 6.43 ± 0.844
1.434AlaHis: 1.434 ± 0.265
5.366AlaIle: 5.366 ± 0.513
6.199AlaLys: 6.199 ± 0.719
7.586AlaLeu: 7.586 ± 0.783
2.729AlaMet: 2.729 ± 0.366
4.024AlaAsn: 4.024 ± 0.565
3.377AlaPro: 3.377 ± 0.652
2.822AlaGln: 2.822 ± 0.388
5.458AlaArg: 5.458 ± 0.598
5.69AlaSer: 5.69 ± 0.649
4.811AlaThr: 4.811 ± 0.515
6.754AlaVal: 6.754 ± 0.701
1.388AlaTrp: 1.388 ± 0.228
2.544AlaTyr: 2.544 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.879CysAla: 0.879 ± 0.277
0.093CysCys: 0.093 ± 0.078
0.463CysAsp: 0.463 ± 0.163
0.601CysGlu: 0.601 ± 0.18
0.139CysPhe: 0.139 ± 0.075
1.064CysGly: 1.064 ± 0.261
0.185CysHis: 0.185 ± 0.092
0.231CysIle: 0.231 ± 0.101
0.416CysLys: 0.416 ± 0.144
0.601CysLeu: 0.601 ± 0.171
0.046CysMet: 0.046 ± 0.043
0.509CysAsn: 0.509 ± 0.16
0.509CysPro: 0.509 ± 0.172
0.37CysGln: 0.37 ± 0.13
0.324CysArg: 0.324 ± 0.137
0.324CysSer: 0.324 ± 0.125
0.648CysThr: 0.648 ± 0.196
0.324CysVal: 0.324 ± 0.128
0.278CysTrp: 0.278 ± 0.127
0.416CysTyr: 0.416 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
6.06AspAla: 6.06 ± 0.637
0.231AspCys: 0.231 ± 0.106
5.597AspAsp: 5.597 ± 0.749
5.366AspGlu: 5.366 ± 0.629
2.452AspPhe: 2.452 ± 0.298
6.291AspGly: 6.291 ± 0.683
1.203AspHis: 1.203 ± 0.216
3.007AspIle: 3.007 ± 0.437
2.729AspLys: 2.729 ± 0.468
6.106AspLeu: 6.106 ± 0.576
1.712AspMet: 1.712 ± 0.244
1.989AspAsn: 1.989 ± 0.281
4.394AspPro: 4.394 ± 0.413
1.85AspGln: 1.85 ± 0.284
3.793AspArg: 3.793 ± 0.411
3.654AspSer: 3.654 ± 0.396
3.516AspThr: 3.516 ± 0.402
3.331AspVal: 3.331 ± 0.333
0.879AspTrp: 0.879 ± 0.252
2.082AspTyr: 2.082 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
7.124GluAla: 7.124 ± 0.645
0.416GluCys: 0.416 ± 0.145
3.932GluAsp: 3.932 ± 0.474
5.32GluGlu: 5.32 ± 0.695
3.331GluPhe: 3.331 ± 0.408
5.69GluGly: 5.69 ± 0.453
1.203GluHis: 1.203 ± 0.321
4.071GluIle: 4.071 ± 0.474
3.654GluLys: 3.654 ± 0.571
6.06GluLeu: 6.06 ± 0.643
1.989GluMet: 1.989 ± 0.313
2.128GluAsn: 2.128 ± 0.309
2.498GluPro: 2.498 ± 0.318
2.729GluGln: 2.729 ± 0.438
3.747GluArg: 3.747 ± 0.539
3.562GluSer: 3.562 ± 0.415
3.146GluThr: 3.146 ± 0.368
4.58GluVal: 4.58 ± 0.479
1.527GluTrp: 1.527 ± 0.335
2.637GluTyr: 2.637 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.683PheAla: 2.683 ± 0.37
0.185PheCys: 0.185 ± 0.089
2.775PheAsp: 2.775 ± 0.378
2.544PheGlu: 2.544 ± 0.407
0.694PhePhe: 0.694 ± 0.181
3.469PheGly: 3.469 ± 0.342
0.601PheHis: 0.601 ± 0.144
1.989PheIle: 1.989 ± 0.273
1.897PheLys: 1.897 ± 0.231
2.498PheLeu: 2.498 ± 0.388
0.925PheMet: 0.925 ± 0.17
1.388PheAsn: 1.388 ± 0.279
1.11PhePro: 1.11 ± 0.247
1.249PheGln: 1.249 ± 0.252
1.989PheArg: 1.989 ± 0.307
1.989PheSer: 1.989 ± 0.28
2.637PheThr: 2.637 ± 0.315
1.804PheVal: 1.804 ± 0.301
0.509PheTrp: 0.509 ± 0.124
0.786PheTyr: 0.786 ± 0.228
0.0PheXaa: 0.0 ± 0.0
Gly
8.049GlyAla: 8.049 ± 0.869
0.555GlyCys: 0.555 ± 0.172
5.782GlyAsp: 5.782 ± 0.578
5.135GlyGlu: 5.135 ± 0.537
3.654GlyPhe: 3.654 ± 0.446
8.141GlyGly: 8.141 ± 1.144
1.48GlyHis: 1.48 ± 0.304
5.088GlyIle: 5.088 ± 0.621
5.181GlyLys: 5.181 ± 0.61
5.597GlyLeu: 5.597 ± 0.629
2.498GlyMet: 2.498 ± 0.427
3.007GlyAsn: 3.007 ± 0.448
3.932GlyPro: 3.932 ± 0.348
3.562GlyGln: 3.562 ± 0.392
5.273GlyArg: 5.273 ± 0.532
5.366GlySer: 5.366 ± 0.613
6.43GlyThr: 6.43 ± 0.594
5.875GlyVal: 5.875 ± 0.536
1.665GlyTrp: 1.665 ± 0.338
2.128GlyTyr: 2.128 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
1.619HisAla: 1.619 ± 0.248
0.324HisCys: 0.324 ± 0.137
0.971HisAsp: 0.971 ± 0.228
1.018HisGlu: 1.018 ± 0.226
0.648HisPhe: 0.648 ± 0.18
1.619HisGly: 1.619 ± 0.22
0.416HisHis: 0.416 ± 0.119
1.156HisIle: 1.156 ± 0.29
0.925HisLys: 0.925 ± 0.23
1.527HisLeu: 1.527 ± 0.209
0.463HisMet: 0.463 ± 0.152
0.879HisAsn: 0.879 ± 0.195
1.156HisPro: 1.156 ± 0.249
0.555HisGln: 0.555 ± 0.172
1.203HisArg: 1.203 ± 0.27
1.018HisSer: 1.018 ± 0.2
0.925HisThr: 0.925 ± 0.197
1.249HisVal: 1.249 ± 0.228
0.509HisTrp: 0.509 ± 0.187
0.74HisTyr: 0.74 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
4.256IleAla: 4.256 ± 0.522
0.786IleCys: 0.786 ± 0.212
3.793IleAsp: 3.793 ± 0.407
4.071IleGlu: 4.071 ± 0.382
1.295IlePhe: 1.295 ± 0.268
4.672IleGly: 4.672 ± 0.564
1.064IleHis: 1.064 ± 0.208
3.192IleIle: 3.192 ± 0.35
3.007IleLys: 3.007 ± 0.399
3.608IleLeu: 3.608 ± 0.545
1.018IleMet: 1.018 ± 0.239
2.822IleAsn: 2.822 ± 0.335
3.053IlePro: 3.053 ± 0.461
2.035IleGln: 2.035 ± 0.33
2.96IleArg: 2.96 ± 0.326
2.96IleSer: 2.96 ± 0.388
3.377IleThr: 3.377 ± 0.474
3.331IleVal: 3.331 ± 0.449
0.833IleTrp: 0.833 ± 0.206
1.434IleTyr: 1.434 ± 0.208
0.0IleXaa: 0.0 ± 0.0
Lys
8.049LysAla: 8.049 ± 0.91
0.416LysCys: 0.416 ± 0.16
3.516LysAsp: 3.516 ± 0.649
3.747LysGlu: 3.747 ± 0.481
1.85LysPhe: 1.85 ± 0.29
3.793LysGly: 3.793 ± 0.364
0.74LysHis: 0.74 ± 0.225
2.359LysIle: 2.359 ± 0.291
4.348LysLys: 4.348 ± 0.573
3.469LysLeu: 3.469 ± 0.385
1.85LysMet: 1.85 ± 0.345
2.452LysAsn: 2.452 ± 0.329
3.238LysPro: 3.238 ± 0.393
1.48LysGln: 1.48 ± 0.32
4.903LysArg: 4.903 ± 0.627
3.053LysSer: 3.053 ± 0.441
2.775LysThr: 2.775 ± 0.348
4.256LysVal: 4.256 ± 0.472
1.249LysTrp: 1.249 ± 0.277
1.85LysTyr: 1.85 ± 0.333
0.0LysXaa: 0.0 ± 0.0
Leu
7.031LeuAla: 7.031 ± 0.589
0.555LeuCys: 0.555 ± 0.172
5.597LeuAsp: 5.597 ± 0.623
5.042LeuGlu: 5.042 ± 0.586
1.804LeuPhe: 1.804 ± 0.314
6.985LeuGly: 6.985 ± 0.521
1.897LeuHis: 1.897 ± 0.379
2.683LeuIle: 2.683 ± 0.296
4.718LeuLys: 4.718 ± 0.627
4.117LeuLeu: 4.117 ± 0.336
1.48LeuMet: 1.48 ± 0.304
3.886LeuAsn: 3.886 ± 0.455
3.747LeuPro: 3.747 ± 0.456
2.128LeuGln: 2.128 ± 0.27
4.626LeuArg: 4.626 ± 0.446
4.903LeuSer: 4.903 ± 0.534
4.071LeuThr: 4.071 ± 0.38
4.996LeuVal: 4.996 ± 0.569
0.694LeuTrp: 0.694 ± 0.211
1.434LeuTyr: 1.434 ± 0.267
0.0LeuXaa: 0.0 ± 0.0
Met
2.96MetAla: 2.96 ± 0.346
0.093MetCys: 0.093 ± 0.073
1.665MetAsp: 1.665 ± 0.3
1.619MetGlu: 1.619 ± 0.313
0.925MetPhe: 0.925 ± 0.206
1.758MetGly: 1.758 ± 0.265
0.324MetHis: 0.324 ± 0.116
1.064MetIle: 1.064 ± 0.185
1.897MetLys: 1.897 ± 0.299
1.804MetLeu: 1.804 ± 0.304
0.555MetMet: 0.555 ± 0.128
0.694MetAsn: 0.694 ± 0.206
1.758MetPro: 1.758 ± 0.33
0.833MetGln: 0.833 ± 0.172
1.064MetArg: 1.064 ± 0.232
1.943MetSer: 1.943 ± 0.324
1.85MetThr: 1.85 ± 0.376
1.295MetVal: 1.295 ± 0.179
0.37MetTrp: 0.37 ± 0.105
0.833MetTyr: 0.833 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
4.857AsnAla: 4.857 ± 0.588
0.231AsnCys: 0.231 ± 0.109
2.313AsnAsp: 2.313 ± 0.313
2.914AsnGlu: 2.914 ± 0.298
1.388AsnPhe: 1.388 ± 0.259
4.487AsnGly: 4.487 ± 0.536
0.971AsnHis: 0.971 ± 0.221
2.498AsnIle: 2.498 ± 0.463
2.035AsnLys: 2.035 ± 0.304
2.914AsnLeu: 2.914 ± 0.444
1.156AsnMet: 1.156 ± 0.225
1.434AsnAsn: 1.434 ± 0.277
2.59AsnPro: 2.59 ± 0.284
1.388AsnGln: 1.388 ± 0.264
2.359AsnArg: 2.359 ± 0.303
1.989AsnSer: 1.989 ± 0.315
1.897AsnThr: 1.897 ± 0.316
2.452AsnVal: 2.452 ± 0.316
0.694AsnTrp: 0.694 ± 0.168
0.925AsnTyr: 0.925 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
3.793ProAla: 3.793 ± 0.547
0.278ProCys: 0.278 ± 0.112
4.209ProAsp: 4.209 ± 0.518
3.562ProGlu: 3.562 ± 0.344
1.573ProPhe: 1.573 ± 0.299
4.811ProGly: 4.811 ± 0.422
1.249ProHis: 1.249 ± 0.295
2.267ProIle: 2.267 ± 0.382
3.978ProLys: 3.978 ± 0.62
3.331ProLeu: 3.331 ± 0.387
1.064ProMet: 1.064 ± 0.244
2.405ProAsn: 2.405 ± 0.306
1.897ProPro: 1.897 ± 0.365
1.341ProGln: 1.341 ± 0.256
2.22ProArg: 2.22 ± 0.315
2.822ProSer: 2.822 ± 0.453
2.498ProThr: 2.498 ± 0.408
2.498ProVal: 2.498 ± 0.369
1.11ProTrp: 1.11 ± 0.208
1.943ProTyr: 1.943 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
3.516GlnAla: 3.516 ± 0.431
0.416GlnCys: 0.416 ± 0.172
1.48GlnAsp: 1.48 ± 0.248
2.498GlnGlu: 2.498 ± 0.275
1.341GlnPhe: 1.341 ± 0.26
1.712GlnGly: 1.712 ± 0.261
0.37GlnHis: 0.37 ± 0.14
2.313GlnIle: 2.313 ± 0.389
2.128GlnLys: 2.128 ± 0.313
2.544GlnLeu: 2.544 ± 0.292
1.249GlnMet: 1.249 ± 0.221
1.712GlnAsn: 1.712 ± 0.363
1.249GlnPro: 1.249 ± 0.283
1.388GlnGln: 1.388 ± 0.294
1.943GlnArg: 1.943 ± 0.248
1.712GlnSer: 1.712 ± 0.236
1.573GlnThr: 1.573 ± 0.258
2.128GlnVal: 2.128 ± 0.247
0.74GlnTrp: 0.74 ± 0.189
1.527GlnTyr: 1.527 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
4.117ArgAla: 4.117 ± 0.553
1.156ArgCys: 1.156 ± 0.288
3.562ArgAsp: 3.562 ± 0.408
3.701ArgGlu: 3.701 ± 0.38
2.313ArgPhe: 2.313 ± 0.293
5.32ArgGly: 5.32 ± 0.437
0.879ArgHis: 0.879 ± 0.222
3.978ArgIle: 3.978 ± 0.419
5.042ArgLys: 5.042 ± 0.573
4.58ArgLeu: 4.58 ± 0.353
1.527ArgMet: 1.527 ± 0.274
2.498ArgAsn: 2.498 ± 0.303
2.59ArgPro: 2.59 ± 0.39
2.498ArgGln: 2.498 ± 0.308
4.533ArgArg: 4.533 ± 0.634
3.516ArgSer: 3.516 ± 0.323
3.007ArgThr: 3.007 ± 0.429
3.192ArgVal: 3.192 ± 0.414
0.925ArgTrp: 0.925 ± 0.21
2.128ArgTyr: 2.128 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
4.903SerAla: 4.903 ± 0.476
0.555SerCys: 0.555 ± 0.163
3.701SerAsp: 3.701 ± 0.448
3.284SerGlu: 3.284 ± 0.358
2.082SerPhe: 2.082 ± 0.291
6.569SerGly: 6.569 ± 0.631
1.295SerHis: 1.295 ± 0.218
3.331SerIle: 3.331 ± 0.438
2.544SerLys: 2.544 ± 0.333
3.469SerLeu: 3.469 ± 0.364
1.712SerMet: 1.712 ± 0.298
2.128SerAsn: 2.128 ± 0.305
2.59SerPro: 2.59 ± 0.349
2.452SerGln: 2.452 ± 0.276
3.608SerArg: 3.608 ± 0.476
3.747SerSer: 3.747 ± 0.559
3.469SerThr: 3.469 ± 0.492
3.608SerVal: 3.608 ± 0.405
1.295SerTrp: 1.295 ± 0.25
1.573SerTyr: 1.573 ± 0.268
0.0SerXaa: 0.0 ± 0.0
Thr
4.209ThrAla: 4.209 ± 0.641
0.37ThrCys: 0.37 ± 0.155
3.839ThrAsp: 3.839 ± 0.478
3.331ThrGlu: 3.331 ± 0.407
1.758ThrPhe: 1.758 ± 0.372
5.505ThrGly: 5.505 ± 0.427
0.971ThrHis: 0.971 ± 0.288
3.053ThrIle: 3.053 ± 0.309
3.053ThrLys: 3.053 ± 0.379
4.765ThrLeu: 4.765 ± 0.383
1.018ThrMet: 1.018 ± 0.221
2.868ThrAsn: 2.868 ± 0.467
3.469ThrPro: 3.469 ± 0.51
1.388ThrGln: 1.388 ± 0.319
3.007ThrArg: 3.007 ± 0.441
2.914ThrSer: 2.914 ± 0.425
3.423ThrThr: 3.423 ± 0.525
4.394ThrVal: 4.394 ± 0.41
1.527ThrTrp: 1.527 ± 0.315
1.619ThrTyr: 1.619 ± 0.226
0.0ThrXaa: 0.0 ± 0.0
Val
5.69ValAla: 5.69 ± 0.583
0.509ValCys: 0.509 ± 0.16
4.024ValAsp: 4.024 ± 0.375
4.533ValGlu: 4.533 ± 0.505
1.85ValPhe: 1.85 ± 0.256
5.875ValGly: 5.875 ± 0.496
1.619ValHis: 1.619 ± 0.321
3.469ValIle: 3.469 ± 0.451
3.608ValLys: 3.608 ± 0.378
3.747ValLeu: 3.747 ± 0.52
1.249ValMet: 1.249 ± 0.259
2.544ValAsn: 2.544 ± 0.342
3.562ValPro: 3.562 ± 0.393
1.85ValGln: 1.85 ± 0.308
4.348ValArg: 4.348 ± 0.489
3.562ValSer: 3.562 ± 0.5
3.839ValThr: 3.839 ± 0.424
3.654ValVal: 3.654 ± 0.501
1.48ValTrp: 1.48 ± 0.315
1.758ValTyr: 1.758 ± 0.288
0.0ValXaa: 0.0 ± 0.0
Trp
1.527TrpAla: 1.527 ± 0.219
0.324TrpCys: 0.324 ± 0.131
1.48TrpAsp: 1.48 ± 0.274
1.11TrpGlu: 1.11 ± 0.271
0.463TrpPhe: 0.463 ± 0.142
1.341TrpGly: 1.341 ± 0.235
0.278TrpHis: 0.278 ± 0.123
0.74TrpIle: 0.74 ± 0.173
0.694TrpLys: 0.694 ± 0.177
1.712TrpLeu: 1.712 ± 0.264
0.416TrpMet: 0.416 ± 0.135
1.018TrpAsn: 1.018 ± 0.246
0.694TrpPro: 0.694 ± 0.172
0.786TrpGln: 0.786 ± 0.196
1.156TrpArg: 1.156 ± 0.289
1.388TrpSer: 1.388 ± 0.239
1.203TrpThr: 1.203 ± 0.293
1.203TrpVal: 1.203 ± 0.214
0.278TrpTrp: 0.278 ± 0.134
0.601TrpTyr: 0.601 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.082TyrAla: 2.082 ± 0.336
0.37TyrCys: 0.37 ± 0.176
2.082TyrAsp: 2.082 ± 0.38
2.082TyrGlu: 2.082 ± 0.358
1.249TyrPhe: 1.249 ± 0.244
2.914TyrGly: 2.914 ± 0.372
0.74TyrHis: 0.74 ± 0.191
1.712TyrIle: 1.712 ± 0.308
1.341TyrLys: 1.341 ± 0.266
2.359TyrLeu: 2.359 ± 0.371
0.601TyrMet: 0.601 ± 0.198
1.11TyrAsn: 1.11 ± 0.25
1.434TyrPro: 1.434 ± 0.315
0.833TyrGln: 0.833 ± 0.201
2.544TyrArg: 2.544 ± 0.375
1.804TyrSer: 1.804 ± 0.237
1.388TyrThr: 1.388 ± 0.25
1.85TyrVal: 1.85 ± 0.311
0.463TyrTrp: 0.463 ± 0.172
0.833TyrTyr: 0.833 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (21619 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski