Salmonella phage vB_SalM_SA002
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 335 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D3UJT9|A0A7D3UJT9_9CAUD Uncharacterized protein OS=Salmonella phage vB_SalM_SA002 OX=2739752 GN=ACSA002_0670 PE=4 SV=1
MM1 pKa = 7.55 PLIVAQCVVTVPSLFGAYY19 pKa = 8.2 ATFGLTNAEE28 pKa = 4.59 LIQLAVDD35 pKa = 3.53 HH36 pKa = 6.17 VNRR39 pKa = 11.84 GDD41 pKa = 3.48 VTFVEE46 pKa = 4.6 HH47 pKa = 7.47 PEE49 pKa = 4.02 YY50 pKa = 10.45 QRR52 pKa = 11.84 SGCAFSNTIVDD63 pKa = 4.3 LAVEE67 pKa = 4.56 ILQLLPAEE75 pKa = 4.93 LEE77 pKa = 4.04 TDD79 pKa = 4.29 FDD81 pKa = 3.65 QLAIIEE87 pKa = 4.33 ISGNTVCLSLEE98 pKa = 4.28 FKK100 pKa = 10.79 DD101 pKa = 3.94
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 4.024
IPC_protein 3.91
Toseland 3.732
ProMoST 3.961
Dawson 3.884
Bjellqvist 4.113
Wikipedia 3.795
Rodwell 3.757
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 4.012
DTASelect 4.151
Thurlkill 3.783
EMBOSS 3.808
Sillero 4.024
Patrickios 0.769
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.987
Protein with the highest isoelectric point:
>tr|A0A7D3QIB8|A0A7D3QIB8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SalM_SA002 OX=2739752 GN=ACSA002_2410 PE=4 SV=1
MM1 pKa = 7.25 VNKK4 pKa = 10.04 QEE6 pKa = 4.19 SLIRR10 pKa = 11.84 MLALANRR17 pKa = 11.84 LNNIVVEE24 pKa = 4.15 MQVRR28 pKa = 11.84 KK29 pKa = 9.63 AQMLARR35 pKa = 11.84 GDD37 pKa = 3.72 TSRR40 pKa = 11.84 KK41 pKa = 8.18 TEE43 pKa = 3.91 PQQ45 pKa = 2.99
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.294
IPC2_protein 9.589
IPC_protein 10.394
Toseland 11.096
ProMoST 11.038
Dawson 11.125
Bjellqvist 10.877
Wikipedia 11.389
Rodwell 11.272
Grimsley 11.14
Solomon 11.389
Lehninger 11.345
Nozaki 11.067
DTASelect 10.877
Thurlkill 11.067
EMBOSS 11.52
Sillero 11.067
Patrickios 11.096
IPC_peptide 11.403
IPC2_peptide 9.75
IPC2.peptide.svr19 9.007
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
335
0
335
89773
39
2216
268.0
29.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.738 ± 0.132
0.943 ± 0.05
6.225 ± 0.105
5.684 ± 0.146
3.606 ± 0.071
7.13 ± 0.284
2.001 ± 0.071
5.939 ± 0.088
5.21 ± 0.114
8.603 ± 0.119
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.081 ± 0.08
5.411 ± 0.096
4.353 ± 0.098
3.843 ± 0.14
4.99 ± 0.105
5.974 ± 0.096
6.525 ± 0.105
7.229 ± 0.12
1.429 ± 0.059
4.084 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here