Candida tenuis (strain ATCC 10573 / BCRC 21748 / CBS 615 / JCM 9827 / NBRC 10315 / NRRL Y-1498 / VKM Y-70) (Yeast) (Yamadazyma tenuis)
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3AZX4|G3AZX4_CANTC GYF domain-containing protein OS=Candida tenuis (strain ATCC 10573 / BCRC 21748 / CBS 615 / JCM 9827 / NBRC 10315 / NRRL Y-1498 / VKM Y-70) OX=590646 GN=CANTEDRAFT_92490 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 9.8 FAAAIALLASVCAVQAADD20 pKa = 3.86 SEE22 pKa = 4.79 DD23 pKa = 3.61 VAFLTNLVQDD33 pKa = 4.54 YY34 pKa = 10.44 KK35 pKa = 11.61 SHH37 pKa = 5.84 TADD40 pKa = 3.06 YY41 pKa = 11.12 AKK43 pKa = 10.53 FIRR46 pKa = 11.84 TASSVPAVITSLALHH61 pKa = 6.21 VATYY65 pKa = 10.47 TDD67 pKa = 3.21 TSYY70 pKa = 7.64 TTLLDD75 pKa = 3.94 NNGLDD80 pKa = 3.61 IDD82 pKa = 4.22 SLEE85 pKa = 4.5 DD86 pKa = 3.66 FATEE90 pKa = 4.09 LPWYY94 pKa = 10.14 SSRR97 pKa = 11.84 LLVADD102 pKa = 4.67 ADD104 pKa = 3.95 ASEE107 pKa = 4.93 SGDD110 pKa = 3.63 ASEE113 pKa = 5.07 TDD115 pKa = 3.35 ASDD118 pKa = 3.56 ASSTEE123 pKa = 3.92 ASSSAASSAASSAASSSAASSSEE146 pKa = 3.7 AGAAAMMAPIGALLGAAAIALMM168 pKa = 4.57
Molecular weight: 16.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.516
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.567
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.706
Sillero 3.859
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|G3BAR6|G3BAR6_CANTC MFS general substrate transporter OS=Candida tenuis (strain ATCC 10573 / BCRC 21748 / CBS 615 / JCM 9827 / NBRC 10315 / NRRL Y-1498 / VKM Y-70) OX=590646 GN=CANTEDRAFT_131515 PE=4 SV=1
MM1 pKa = 7.72 AGLSTVVASLNRR13 pKa = 11.84 KK14 pKa = 8.54 VVTSSSRR21 pKa = 11.84 PTVLRR26 pKa = 11.84 NMAGLSTSVTLGVVGLTVSGKK47 pKa = 7.11 MVRR50 pKa = 11.84 TSTLVTGGRR59 pKa = 11.84 RR60 pKa = 11.84 LEE62 pKa = 4.14 LRR64 pKa = 11.84 SSRR67 pKa = 11.84 SRR69 pKa = 11.84 RR70 pKa = 11.84 WLFSSRR76 pKa = 11.84 SIAVLRR82 pKa = 11.84 YY83 pKa = 8.35 VAKK86 pKa = 9.34 LTTIVTLGTLFLSWTVRR103 pKa = 11.84 LNMSHH108 pKa = 6.75 ITTQVTLFMGGQFWFLTFF126 pKa = 4.14
Molecular weight: 13.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 10.921
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.135
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.871
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.12
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5540
511
6051
2636551
51
4087
435.7
49.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.588 ± 0.032
1.163 ± 0.011
5.977 ± 0.023
6.218 ± 0.026
4.794 ± 0.021
5.397 ± 0.03
2.205 ± 0.014
6.563 ± 0.024
6.915 ± 0.025
9.707 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.032 ± 0.01
5.678 ± 0.03
4.439 ± 0.025
3.769 ± 0.017
4.134 ± 0.019
8.874 ± 0.032
5.542 ± 0.019
6.303 ± 0.024
1.075 ± 0.01
3.629 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here