Amino acid dipepetide frequency for Lactobacillus sakei subsp. sakei (strain 23K)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.102AlaAla: 8.102 ± 0.163
0.397AlaCys: 0.397 ± 0.028
5.327AlaAsp: 5.327 ± 0.111
4.929AlaGlu: 4.929 ± 0.123
3.407AlaPhe: 3.407 ± 0.089
6.285AlaGly: 6.285 ± 0.113
1.686AlaHis: 1.686 ± 0.062
6.761AlaIle: 6.761 ± 0.13
5.549AlaLys: 5.549 ± 0.125
8.714AlaLeu: 8.714 ± 0.142
2.3AlaMet: 2.3 ± 0.068
3.883AlaAsn: 3.883 ± 0.097
2.88AlaPro: 2.88 ± 0.087
4.741AlaGln: 4.741 ± 0.103
2.83AlaArg: 2.83 ± 0.071
4.49AlaSer: 4.49 ± 0.109
5.868AlaThr: 5.868 ± 0.112
6.324AlaVal: 6.324 ± 0.139
0.664AlaTrp: 0.664 ± 0.042
2.699AlaTyr: 2.699 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.38CysAla: 0.38 ± 0.028
0.055CysCys: 0.055 ± 0.012
0.271CysAsp: 0.271 ± 0.025
0.275CysGlu: 0.275 ± 0.024
0.214CysPhe: 0.214 ± 0.02
0.469CysGly: 0.469 ± 0.033
0.157CysHis: 0.157 ± 0.019
0.243CysIle: 0.243 ± 0.021
0.124CysLys: 0.124 ± 0.014
0.66CysLeu: 0.66 ± 0.039
0.092CysMet: 0.092 ± 0.013
0.131CysAsn: 0.131 ± 0.015
0.231CysPro: 0.231 ± 0.026
0.319CysGln: 0.319 ± 0.028
0.188CysArg: 0.188 ± 0.02
0.24CysSer: 0.24 ± 0.019
0.249CysThr: 0.249 ± 0.021
0.323CysVal: 0.323 ± 0.026
0.042CysTrp: 0.042 ± 0.011
0.205CysTyr: 0.205 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.364AspAla: 4.364 ± 0.1
0.297AspCys: 0.297 ± 0.029
2.966AspAsp: 2.966 ± 0.086
3.429AspGlu: 3.429 ± 0.097
2.691AspPhe: 2.691 ± 0.071
3.531AspGly: 3.531 ± 0.099
1.28AspHis: 1.28 ± 0.054
3.485AspIle: 3.485 ± 0.109
2.758AspLys: 2.758 ± 0.084
5.626AspLeu: 5.626 ± 0.114
1.428AspMet: 1.428 ± 0.053
2.243AspAsn: 2.243 ± 0.066
2.07AspPro: 2.07 ± 0.056
3.529AspGln: 3.529 ± 0.094
2.171AspArg: 2.171 ± 0.069
2.736AspSer: 2.736 ± 0.082
2.752AspThr: 2.752 ± 0.087
3.632AspVal: 3.632 ± 0.096
0.815AspTrp: 0.815 ± 0.038
2.584AspTyr: 2.584 ± 0.07
0.0AspXaa: 0.0 ± 0.0
Glu
5.243GluAla: 5.243 ± 0.117
0.194GluCys: 0.194 ± 0.019
2.675GluAsp: 2.675 ± 0.07
3.274GluGlu: 3.274 ± 0.107
1.867GluPhe: 1.867 ± 0.069
2.822GluGly: 2.822 ± 0.083
1.096GluHis: 1.096 ± 0.042
4.103GluIle: 4.103 ± 0.108
3.922GluLys: 3.922 ± 0.088
5.517GluLeu: 5.517 ± 0.116
1.852GluMet: 1.852 ± 0.062
2.512GluAsn: 2.512 ± 0.081
1.817GluPro: 1.817 ± 0.062
3.555GluGln: 3.555 ± 0.105
2.608GluArg: 2.608 ± 0.077
2.745GluSer: 2.745 ± 0.083
3.901GluThr: 3.901 ± 0.102
3.783GluVal: 3.783 ± 0.111
0.505GluTrp: 0.505 ± 0.033
1.546GluTyr: 1.546 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.151PheAla: 3.151 ± 0.094
0.288PheCys: 0.288 ± 0.024
2.501PheAsp: 2.501 ± 0.075
2.678PheGlu: 2.678 ± 0.072
1.933PhePhe: 1.933 ± 0.073
3.204PheGly: 3.204 ± 0.085
0.64PheHis: 0.64 ± 0.035
3.088PheIle: 3.088 ± 0.085
2.868PheLys: 2.868 ± 0.074
3.588PheLeu: 3.588 ± 0.102
1.19PheMet: 1.19 ± 0.052
2.037PheAsn: 2.037 ± 0.07
1.356PhePro: 1.356 ± 0.047
1.337PheGln: 1.337 ± 0.05
1.199PheArg: 1.199 ± 0.046
2.732PheSer: 2.732 ± 0.074
2.328PheThr: 2.328 ± 0.073
2.918PheVal: 2.918 ± 0.092
0.491PheTrp: 0.491 ± 0.033
1.658PheTyr: 1.658 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
5.228GlyAla: 5.228 ± 0.113
0.402GlyCys: 0.402 ± 0.03
3.285GlyAsp: 3.285 ± 0.086
3.252GlyGlu: 3.252 ± 0.076
2.968GlyPhe: 2.968 ± 0.08
4.449GlyGly: 4.449 ± 0.103
1.544GlyHis: 1.544 ± 0.054
5.469GlyIle: 5.469 ± 0.119
3.604GlyLys: 3.604 ± 0.09
7.087GlyLeu: 7.087 ± 0.147
1.9GlyMet: 1.9 ± 0.069
2.461GlyAsn: 2.461 ± 0.083
1.642GlyPro: 1.642 ± 0.061
4.021GlyGln: 4.021 ± 0.114
2.782GlyArg: 2.782 ± 0.077
3.695GlySer: 3.695 ± 0.091
4.132GlyThr: 4.132 ± 0.111
4.975GlyVal: 4.975 ± 0.098
0.714GlyTrp: 0.714 ± 0.04
2.634GlyTyr: 2.634 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
1.356HisAla: 1.356 ± 0.05
0.114HisCys: 0.114 ± 0.016
1.101HisAsp: 1.101 ± 0.047
1.11HisGlu: 1.11 ± 0.045
1.144HisPhe: 1.144 ± 0.051
1.247HisGly: 1.247 ± 0.043
0.657HisHis: 0.657 ± 0.037
1.256HisIle: 1.256 ± 0.054
0.909HisLys: 0.909 ± 0.041
2.228HisLeu: 2.228 ± 0.066
0.533HisMet: 0.533 ± 0.028
0.837HisAsn: 0.837 ± 0.037
0.994HisPro: 0.994 ± 0.043
1.267HisGln: 1.267 ± 0.054
0.832HisArg: 0.832 ± 0.038
0.956HisSer: 0.956 ± 0.043
0.957HisThr: 0.957 ± 0.045
1.319HisVal: 1.319 ± 0.045
0.256HisTrp: 0.256 ± 0.024
1.035HisTyr: 1.035 ± 0.048
0.0HisXaa: 0.0 ± 0.0
Ile
6.68IleAla: 6.68 ± 0.113
0.518IleCys: 0.518 ± 0.033
4.342IleAsp: 4.342 ± 0.103
4.298IleGlu: 4.298 ± 0.1
2.843IlePhe: 2.843 ± 0.078
5.755IleGly: 5.755 ± 0.142
1.179IleHis: 1.179 ± 0.048
5.298IleIle: 5.298 ± 0.142
4.045IleLys: 4.045 ± 0.098
6.798IleLeu: 6.798 ± 0.141
1.911IleMet: 1.911 ± 0.069
3.363IleAsn: 3.363 ± 0.087
2.66IlePro: 2.66 ± 0.082
3.125IleGln: 3.125 ± 0.076
2.466IleArg: 2.466 ± 0.077
4.285IleSer: 4.285 ± 0.102
4.414IleThr: 4.414 ± 0.085
5.056IleVal: 5.056 ± 0.12
0.631IleTrp: 0.631 ± 0.035
2.12IleTyr: 2.12 ± 0.067
0.0IleXaa: 0.0 ± 0.0
Lys
5.346LysAla: 5.346 ± 0.111
0.122LysCys: 0.122 ± 0.015
2.977LysAsp: 2.977 ± 0.083
3.494LysGlu: 3.494 ± 0.089
1.551LysPhe: 1.551 ± 0.053
3.26LysGly: 3.26 ± 0.08
1.044LysHis: 1.044 ± 0.043
4.062LysIle: 4.062 ± 0.097
4.466LysLys: 4.466 ± 0.121
5.04LysLeu: 5.04 ± 0.119
2.177LysMet: 2.177 ± 0.067
2.677LysAsn: 2.677 ± 0.081
2.177LysPro: 2.177 ± 0.068
3.51LysGln: 3.51 ± 0.093
2.983LysArg: 2.983 ± 0.083
2.975LysSer: 2.975 ± 0.091
4.364LysThr: 4.364 ± 0.099
4.108LysVal: 4.108 ± 0.098
0.609LysTrp: 0.609 ± 0.038
1.727LysTyr: 1.727 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
10.144LeuAla: 10.144 ± 0.167
0.481LeuCys: 0.481 ± 0.035
5.115LeuAsp: 5.115 ± 0.122
5.106LeuGlu: 5.106 ± 0.104
4.257LeuPhe: 4.257 ± 0.118
6.453LeuGly: 6.453 ± 0.128
1.62LeuHis: 1.62 ± 0.054
7.441LeuIle: 7.441 ± 0.147
6.482LeuLys: 6.482 ± 0.102
10.402LeuLeu: 10.402 ± 0.211
2.92LeuMet: 2.92 ± 0.077
4.639LeuAsn: 4.639 ± 0.111
4.341LeuPro: 4.341 ± 0.091
4.234LeuGln: 4.234 ± 0.106
3.486LeuArg: 3.486 ± 0.088
6.165LeuSer: 6.165 ± 0.118
7.657LeuThr: 7.657 ± 0.133
7.451LeuVal: 7.451 ± 0.136
0.808LeuTrp: 0.808 ± 0.041
2.806LeuTyr: 2.806 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.865MetAla: 2.865 ± 0.065
0.114MetCys: 0.114 ± 0.016
1.236MetAsp: 1.236 ± 0.05
0.926MetGlu: 0.926 ± 0.047
0.806MetPhe: 0.806 ± 0.038
1.931MetGly: 1.931 ± 0.07
0.498MetHis: 0.498 ± 0.029
1.976MetIle: 1.976 ± 0.077
1.592MetLys: 1.592 ± 0.062
2.623MetLeu: 2.623 ± 0.074
1.011MetMet: 1.011 ± 0.048
1.306MetAsn: 1.306 ± 0.051
1.229MetPro: 1.229 ± 0.051
1.496MetGln: 1.496 ± 0.054
1.153MetArg: 1.153 ± 0.051
1.677MetSer: 1.677 ± 0.054
2.402MetThr: 2.402 ± 0.059
1.918MetVal: 1.918 ± 0.058
0.173MetTrp: 0.173 ± 0.018
0.603MetTyr: 0.603 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.306AsnAla: 3.306 ± 0.085
0.253AsnCys: 0.253 ± 0.024
2.479AsnAsp: 2.479 ± 0.081
2.472AsnGlu: 2.472 ± 0.069
1.69AsnPhe: 1.69 ± 0.055
3.193AsnGly: 3.193 ± 0.087
1.147AsnHis: 1.147 ± 0.041
2.835AsnIle: 2.835 ± 0.084
2.297AsnLys: 2.297 ± 0.075
4.114AsnLeu: 4.114 ± 0.092
1.088AsnMet: 1.088 ± 0.049
2.016AsnAsn: 2.016 ± 0.082
2.169AsnPro: 2.169 ± 0.069
2.955AsnGln: 2.955 ± 0.082
2.022AsnArg: 2.022 ± 0.064
2.079AsnSer: 2.079 ± 0.075
2.402AsnThr: 2.402 ± 0.081
2.798AsnVal: 2.798 ± 0.08
0.594AsnTrp: 0.594 ± 0.041
1.594AsnTyr: 1.594 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
3.248ProAla: 3.248 ± 0.094
0.129ProCys: 0.129 ± 0.016
2.286ProAsp: 2.286 ± 0.07
3.178ProGlu: 3.178 ± 0.096
1.649ProPhe: 1.649 ± 0.062
2.144ProGly: 2.144 ± 0.066
0.67ProHis: 0.67 ± 0.033
2.828ProIle: 2.828 ± 0.082
2.167ProLys: 2.167 ± 0.076
3.719ProLeu: 3.719 ± 0.098
0.825ProMet: 0.825 ± 0.04
1.76ProAsn: 1.76 ± 0.057
0.568ProPro: 0.568 ± 0.037
1.747ProGln: 1.747 ± 0.069
1.162ProArg: 1.162 ± 0.046
1.824ProSer: 1.824 ± 0.066
2.804ProThr: 2.804 ± 0.086
3.003ProVal: 3.003 ± 0.079
0.295ProTrp: 0.295 ± 0.025
1.384ProTyr: 1.384 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
5.237GlnAla: 5.237 ± 0.13
0.155GlnCys: 0.155 ± 0.018
2.291GlnAsp: 2.291 ± 0.072
2.625GlnGlu: 2.625 ± 0.083
2.243GlnPhe: 2.243 ± 0.058
2.5GlnGly: 2.5 ± 0.07
1.061GlnHis: 1.061 ± 0.047
3.656GlnIle: 3.656 ± 0.095
3.606GlnLys: 3.606 ± 0.089
6.534GlnLeu: 6.534 ± 0.155
1.502GlnMet: 1.502 ± 0.054
2.306GlnAsn: 2.306 ± 0.078
2.227GlnPro: 2.227 ± 0.07
3.771GlnGln: 3.771 ± 0.14
2.25GlnArg: 2.25 ± 0.072
2.83GlnSer: 2.83 ± 0.085
3.972GlnThr: 3.972 ± 0.101
3.739GlnVal: 3.739 ± 0.091
0.485GlnTrp: 0.485 ± 0.03
1.789GlnTyr: 1.789 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
2.756ArgAla: 2.756 ± 0.085
0.162ArgCys: 0.162 ± 0.016
2.108ArgAsp: 2.108 ± 0.07
2.332ArgGlu: 2.332 ± 0.08
1.878ArgPhe: 1.878 ± 0.057
2.19ArgGly: 2.19 ± 0.069
1.024ArgHis: 1.024 ± 0.047
2.677ArgIle: 2.677 ± 0.08
2.134ArgLys: 2.134 ± 0.075
4.388ArgLeu: 4.388 ± 0.098
1.123ArgMet: 1.123 ± 0.046
1.548ArgAsn: 1.548 ± 0.056
1.461ArgPro: 1.461 ± 0.067
2.752ArgGln: 2.752 ± 0.086
2.048ArgArg: 2.048 ± 0.074
1.769ArgSer: 1.769 ± 0.062
2.064ArgThr: 2.064 ± 0.065
2.584ArgVal: 2.584 ± 0.067
0.341ArgTrp: 0.341 ± 0.024
1.813ArgTyr: 1.813 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
4.425SerAla: 4.425 ± 0.099
0.231SerCys: 0.231 ± 0.017
3.173SerAsp: 3.173 ± 0.072
3.232SerGlu: 3.232 ± 0.093
2.461SerPhe: 2.461 ± 0.073
4.162SerGly: 4.162 ± 0.095
1.19SerHis: 1.19 ± 0.055
3.811SerIle: 3.811 ± 0.087
2.78SerLys: 2.78 ± 0.083
5.523SerLeu: 5.523 ± 0.113
1.459SerMet: 1.459 ± 0.053
2.081SerAsn: 2.081 ± 0.073
1.728SerPro: 1.728 ± 0.051
2.944SerGln: 2.944 ± 0.081
2.28SerArg: 2.28 ± 0.063
3.079SerSer: 3.079 ± 0.114
3.112SerThr: 3.112 ± 0.087
3.959SerVal: 3.959 ± 0.099
0.57SerTrp: 0.57 ± 0.042
1.954SerTyr: 1.954 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
5.859ThrAla: 5.859 ± 0.123
0.269ThrCys: 0.269 ± 0.028
3.89ThrAsp: 3.89 ± 0.087
3.35ThrGlu: 3.35 ± 0.075
2.64ThrPhe: 2.64 ± 0.078
4.61ThrGly: 4.61 ± 0.115
1.297ThrHis: 1.297 ± 0.051
4.992ThrIle: 4.992 ± 0.103
3.841ThrLys: 3.841 ± 0.095
6.661ThrLeu: 6.661 ± 0.127
1.391ThrMet: 1.391 ± 0.055
2.931ThrAsn: 2.931 ± 0.092
3.167ThrPro: 3.167 ± 0.095
2.905ThrGln: 2.905 ± 0.085
2.077ThrArg: 2.077 ± 0.056
3.39ThrSer: 3.39 ± 0.1
4.885ThrThr: 4.885 ± 0.137
5.156ThrVal: 5.156 ± 0.107
0.623ThrTrp: 0.623 ± 0.039
2.016ThrTyr: 2.016 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
6.916ValAla: 6.916 ± 0.144
0.402ValCys: 0.402 ± 0.029
4.032ValAsp: 4.032 ± 0.099
3.485ValGlu: 3.485 ± 0.087
2.747ValPhe: 2.747 ± 0.082
5.088ValGly: 5.088 ± 0.112
1.107ValHis: 1.107 ± 0.046
5.324ValIle: 5.324 ± 0.11
3.997ValLys: 3.997 ± 0.112
7.233ValLeu: 7.233 ± 0.117
1.839ValMet: 1.839 ± 0.059
3.119ValAsn: 3.119 ± 0.088
2.891ValPro: 2.891 ± 0.074
2.998ValGln: 2.998 ± 0.078
2.302ValArg: 2.302 ± 0.076
4.215ValSer: 4.215 ± 0.084
5.2ValThr: 5.2 ± 0.123
5.368ValVal: 5.368 ± 0.11
0.574ValTrp: 0.574 ± 0.037
2.195ValTyr: 2.195 ± 0.07
0.0ValXaa: 0.0 ± 0.0
Trp
0.664TrpAla: 0.664 ± 0.039
0.079TrpCys: 0.079 ± 0.013
0.47TrpAsp: 0.47 ± 0.032
0.363TrpGlu: 0.363 ± 0.027
0.457TrpPhe: 0.457 ± 0.037
0.657TrpGly: 0.657 ± 0.041
0.347TrpHis: 0.347 ± 0.027
0.563TrpIle: 0.563 ± 0.032
0.243TrpLys: 0.243 ± 0.022
1.478TrpLeu: 1.478 ± 0.071
0.216TrpMet: 0.216 ± 0.022
0.341TrpAsn: 0.341 ± 0.027
0.349TrpPro: 0.349 ± 0.027
0.869TrpGln: 0.869 ± 0.045
0.515TrpArg: 0.515 ± 0.029
0.52TrpSer: 0.52 ± 0.032
0.531TrpThr: 0.531 ± 0.037
0.577TrpVal: 0.577 ± 0.03
0.142TrpTrp: 0.142 ± 0.018
0.369TrpTyr: 0.369 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.566TyrAla: 2.566 ± 0.072
0.205TyrCys: 0.205 ± 0.019
1.859TyrAsp: 1.859 ± 0.061
1.728TyrGlu: 1.728 ± 0.062
1.74TyrPhe: 1.74 ± 0.063
2.298TyrGly: 2.298 ± 0.067
0.869TyrHis: 0.869 ± 0.041
1.92TyrIle: 1.92 ± 0.057
1.284TyrLys: 1.284 ± 0.049
4.075TyrLeu: 4.075 ± 0.087
0.777TyrMet: 0.777 ± 0.036
1.312TyrAsn: 1.312 ± 0.054
1.435TyrPro: 1.435 ± 0.057
2.702TyrGln: 2.702 ± 0.072
1.799TyrArg: 1.799 ± 0.067
1.751TyrSer: 1.751 ± 0.059
1.955TyrThr: 1.955 ± 0.065
2.038TyrVal: 2.038 ± 0.054
0.417TyrTrp: 0.417 ± 0.03
1.395TyrTyr: 1.395 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1872 proteins (542104 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski