Grapevine leafroll-associated virus 3 (isolate United States/NY1) (GLRaV-3) (Grapevine leafroll-associated closterovirus (isolate 109))

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus; Grapevine leafroll-associated virus 3

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|O71194|P20A_GLRV3 Protein P20A OS=Grapevine leafroll-associated virus 3 (isolate United States/NY1) OX=651354 GN=ORF9 PE=3 SV=1
MM1 pKa = 7.76EE2 pKa = 5.34FRR4 pKa = 11.84PVLITVRR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 3.18PGVNTGSLKK22 pKa = 10.72VIAYY26 pKa = 8.3DD27 pKa = 3.32LHH29 pKa = 7.63YY30 pKa = 11.44DD31 pKa = 4.46NIFDD35 pKa = 3.71NCAVKK40 pKa = 10.48SFRR43 pKa = 11.84DD44 pKa = 3.36TDD46 pKa = 3.53TGFTVMKK53 pKa = 9.95EE54 pKa = 3.97YY55 pKa = 9.76STNSAFILSPYY66 pKa = 10.25KK67 pKa = 10.65LFSAVFNKK75 pKa = 9.74EE76 pKa = 3.29GEE78 pKa = 4.35MISNDD83 pKa = 3.1VGSSFRR89 pKa = 11.84VYY91 pKa = 10.88NIFSQMCKK99 pKa = 10.21DD100 pKa = 3.14INEE103 pKa = 3.82ISEE106 pKa = 4.11IQRR109 pKa = 11.84AGYY112 pKa = 10.35LEE114 pKa = 4.65TYY116 pKa = 10.25LGDD119 pKa = 3.87GQADD123 pKa = 3.38TDD125 pKa = 3.65IFFDD129 pKa = 3.76VLTNNKK135 pKa = 9.96AKK137 pKa = 10.45VRR139 pKa = 11.84WLVNKK144 pKa = 10.06DD145 pKa = 2.9HH146 pKa = 6.72SAWCGILNDD155 pKa = 4.6LKK157 pKa = 10.74WEE159 pKa = 4.06EE160 pKa = 4.25SNKK163 pKa = 10.4EE164 pKa = 3.89KK165 pKa = 10.96FKK167 pKa = 11.24GRR169 pKa = 11.84DD170 pKa = 3.12ILDD173 pKa = 3.85TYY175 pKa = 10.91VLSSDD180 pKa = 3.81YY181 pKa = 11.11PGFKK185 pKa = 10.41

Molecular weight: 21.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|O41518|CAPSD_GLRV3 Capsid protein OS=Grapevine leafroll-associated virus 3 (isolate United States/NY1) OX=651354 GN=ORF6 PE=3 SV=1
MM1 pKa = 8.07DD2 pKa = 4.6KK3 pKa = 10.7YY4 pKa = 10.96IYY6 pKa = 9.13VTGILNPNEE15 pKa = 3.77ARR17 pKa = 11.84DD18 pKa = 3.75EE19 pKa = 4.32VFSVVNKK26 pKa = 10.52GYY28 pKa = 9.88IGPGGRR34 pKa = 11.84SFSNRR39 pKa = 11.84GSKK42 pKa = 8.45YY43 pKa = 7.87TVVWEE48 pKa = 4.05NSAARR53 pKa = 11.84ISGFTSTSQSTIDD66 pKa = 3.21AFAYY70 pKa = 10.13FLLKK74 pKa = 10.81GGLTTTLSNPINCEE88 pKa = 3.16NWVRR92 pKa = 11.84SSKK95 pKa = 10.71DD96 pKa = 2.89LSAFFRR102 pKa = 11.84TLIKK106 pKa = 10.67GKK108 pKa = 9.7IYY110 pKa = 10.66ASRR113 pKa = 11.84SVDD116 pKa = 3.41SNLPKK121 pKa = 9.96KK122 pKa = 10.69DD123 pKa = 3.69RR124 pKa = 11.84DD125 pKa = 4.38DD126 pKa = 3.37IMEE129 pKa = 4.61ASRR132 pKa = 11.84RR133 pKa = 11.84LSPSDD138 pKa = 3.37AAFCRR143 pKa = 11.84AVSVQVGKK151 pKa = 10.89YY152 pKa = 10.2VDD154 pKa = 3.41VTQNLEE160 pKa = 4.1STIVPLRR167 pKa = 11.84VMEE170 pKa = 4.09IKK172 pKa = 10.2KK173 pKa = 10.19RR174 pKa = 11.84RR175 pKa = 11.84GSAHH179 pKa = 5.4VSLPKK184 pKa = 10.41VVSAYY189 pKa = 10.95VDD191 pKa = 4.5FYY193 pKa = 11.52TNLQEE198 pKa = 4.35LLSDD202 pKa = 3.51EE203 pKa = 4.4VTRR206 pKa = 11.84ARR208 pKa = 11.84TDD210 pKa = 3.3TVSAYY215 pKa = 9.31ATDD218 pKa = 3.48SMAFLVKK225 pKa = 10.01MLPLTARR232 pKa = 11.84EE233 pKa = 3.85QWLKK237 pKa = 11.32DD238 pKa = 3.23VLGYY242 pKa = 10.93LLVRR246 pKa = 11.84RR247 pKa = 11.84RR248 pKa = 11.84PANFSYY254 pKa = 10.49DD255 pKa = 3.43VRR257 pKa = 11.84VAWVYY262 pKa = 11.26DD263 pKa = 3.89VIATLKK269 pKa = 10.67LVIRR273 pKa = 11.84LFFNKK278 pKa = 8.3DD279 pKa = 2.99TPGGIKK285 pKa = 9.87DD286 pKa = 4.75LKK288 pKa = 8.83PCVPIEE294 pKa = 4.24SFDD297 pKa = 3.87PFHH300 pKa = 6.71EE301 pKa = 4.49LSSYY305 pKa = 10.52FSRR308 pKa = 11.84LSYY311 pKa = 11.32EE312 pKa = 3.79MTTGKK317 pKa = 10.25GGKK320 pKa = 8.56ICPEE324 pKa = 3.53IAEE327 pKa = 4.28KK328 pKa = 10.46LVRR331 pKa = 11.84RR332 pKa = 11.84LMEE335 pKa = 4.26EE336 pKa = 3.83NYY338 pKa = 10.34KK339 pKa = 10.78LRR341 pKa = 11.84LTPVMALIIILVYY354 pKa = 9.77YY355 pKa = 10.13SIYY358 pKa = 9.0GTNATRR364 pKa = 11.84IKK366 pKa = 10.37RR367 pKa = 11.84RR368 pKa = 11.84PDD370 pKa = 3.28FLNVRR375 pKa = 11.84IKK377 pKa = 11.08GRR379 pKa = 11.84VEE381 pKa = 3.8KK382 pKa = 10.97VSLRR386 pKa = 11.84GVEE389 pKa = 4.03DD390 pKa = 3.31RR391 pKa = 11.84AFRR394 pKa = 11.84ISEE397 pKa = 3.84KK398 pKa = 10.58RR399 pKa = 11.84GINAQRR405 pKa = 11.84VLCRR409 pKa = 11.84YY410 pKa = 10.02YY411 pKa = 11.36SDD413 pKa = 3.73LTCLARR419 pKa = 11.84RR420 pKa = 11.84HH421 pKa = 5.07YY422 pKa = 10.62GIRR425 pKa = 11.84RR426 pKa = 11.84NNWKK430 pKa = 8.2TLSYY434 pKa = 11.39VDD436 pKa = 3.46GTLAYY441 pKa = 8.18DD442 pKa = 3.61TADD445 pKa = 4.21CITSKK450 pKa = 10.3VRR452 pKa = 11.84NTINTADD459 pKa = 3.63HH460 pKa = 6.8ASIIHH465 pKa = 5.83YY466 pKa = 10.35IKK468 pKa = 10.13TNEE471 pKa = 3.99NQVTGTTLPHH481 pKa = 5.83QLL483 pKa = 3.71

Molecular weight: 54.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

7560

36

2772

581.5

64.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.222 ± 0.319

1.865 ± 0.149

5.582 ± 0.247

5.146 ± 0.201

5.053 ± 0.336

7.222 ± 0.438

1.574 ± 0.246

4.669 ± 0.565

5.952 ± 0.253

8.452 ± 0.636

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.157

4.021 ± 0.337

3.81 ± 0.3

2.5 ± 0.24

5.952 ± 0.378

8.598 ± 0.648

6.111 ± 0.315

10.04 ± 0.428

0.728 ± 0.099

3.624 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski