Methanobacterium congolense

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D3L4I2|A0A1D3L4I2_9EURY Uncharacterized protein OS=Methanobacterium congolense OX=118062 GN=MCBB_1831 PE=4 SV=1
MM1 pKa = 6.73YY2 pKa = 9.63RR3 pKa = 11.84KK4 pKa = 9.35HH5 pKa = 6.49RR6 pKa = 11.84RR7 pKa = 11.84PAFLLLTVFFVICAAGAAAAQEE29 pKa = 4.11AVDD32 pKa = 4.0VNITKK37 pKa = 8.07TAPDD41 pKa = 3.58TVVAGDD47 pKa = 3.86NITYY51 pKa = 10.5NITVTNTNPDD61 pKa = 3.24EE62 pKa = 4.54DD63 pKa = 4.15AQAVTLVDD71 pKa = 4.12DD72 pKa = 4.14VTGKK76 pKa = 10.67LVDD79 pKa = 3.68PQYY82 pKa = 11.62SVDD85 pKa = 4.05GVDD88 pKa = 2.89KK89 pKa = 11.14GSWNGTHH96 pKa = 6.33IWDD99 pKa = 3.91VLPAQTSVLVKK110 pKa = 9.93IWGQVNPSQPAGPWLYY126 pKa = 11.32NDD128 pKa = 3.5ATVFSANDD136 pKa = 3.64PNQDD140 pKa = 2.75NNYY143 pKa = 8.51AHH145 pKa = 6.83TWTLVSTAADD155 pKa = 3.7LNITKK160 pKa = 7.98TAPDD164 pKa = 3.5TVVAGNNITYY174 pKa = 10.25DD175 pKa = 2.93IVVANNGPSDD185 pKa = 3.76AQAVTLVDD193 pKa = 4.12DD194 pKa = 4.18VTGKK198 pKa = 10.76LINPQFSIDD207 pKa = 3.75GVNMGSWGGAYY218 pKa = 9.95GGSYY222 pKa = 9.92VWDD225 pKa = 3.4VLAAQTSVLVKK236 pKa = 9.93IWGQVNPSQPAGPWLYY252 pKa = 11.32NDD254 pKa = 3.35ATVFSVTDD262 pKa = 3.92PNQDD266 pKa = 2.62NNYY269 pKa = 8.42AHH271 pKa = 6.71TWTWVYY277 pKa = 10.15TLADD281 pKa = 3.59VYY283 pKa = 9.96VQKK286 pKa = 9.45TAPANVTAGEE296 pKa = 4.33NITYY300 pKa = 10.44NIVVGNNGPSDD311 pKa = 3.83AQNVTVTDD319 pKa = 4.18TLDD322 pKa = 3.16SWLSGATYY330 pKa = 9.88TLDD333 pKa = 3.76GADD336 pKa = 3.37MGSWTGSLSLGTLAPGKK353 pKa = 9.96YY354 pKa = 6.57VTIIITGLVNATAPAGSLIGNTVSAITDD382 pKa = 3.47TSDD385 pKa = 3.35PFMDD389 pKa = 4.05NNTASTVTGVVTATSGGEE407 pKa = 4.19GVLPPISGGEE417 pKa = 3.95AVTEE421 pKa = 4.21EE422 pKa = 4.37PLSGGEE428 pKa = 3.94AVSAEE433 pKa = 4.62TIPMEE438 pKa = 4.5TTGSPVTLLVMALLVLLASLSGIRR462 pKa = 11.84RR463 pKa = 11.84SS464 pKa = 3.4

Molecular weight: 48.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D3L3Z8|A0A1D3L3Z8_9EURY Biopolymer transport protein OS=Methanobacterium congolense OX=118062 GN=MCBB_1823 PE=3 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84NKK5 pKa = 9.92PLAKK9 pKa = 10.08KK10 pKa = 10.28LRR12 pKa = 11.84LAKK15 pKa = 10.27AGKK18 pKa = 7.75QNRR21 pKa = 11.84RR22 pKa = 11.84VPLWVMLKK30 pKa = 9.19TSRR33 pKa = 11.84KK34 pKa = 8.41VRR36 pKa = 11.84AHH38 pKa = 6.02PKK40 pKa = 7.21MRR42 pKa = 11.84QWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 11.06VKK50 pKa = 10.52AA51 pKa = 3.54

Molecular weight: 6.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2340

0

2340

662468

29

2816

283.1

31.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.491 ± 0.059

1.197 ± 0.029

5.653 ± 0.045

7.501 ± 0.084

4.181 ± 0.047

7.234 ± 0.052

1.754 ± 0.023

8.057 ± 0.058

7.422 ± 0.068

9.145 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.025

4.756 ± 0.063

3.747 ± 0.034

2.339 ± 0.023

3.949 ± 0.039

6.309 ± 0.047

5.414 ± 0.076

7.557 ± 0.049

0.873 ± 0.019

3.495 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski