Amino acid dipepetide frequency for Methanobacterium congolense

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.3AlaAla: 5.3 ± 0.122
0.85AlaCys: 0.85 ± 0.044
3.722AlaAsp: 3.722 ± 0.082
4.681AlaGlu: 4.681 ± 0.1
2.738AlaPhe: 2.738 ± 0.076
5.066AlaGly: 5.066 ± 0.103
1.097AlaHis: 1.097 ± 0.047
4.861AlaIle: 4.861 ± 0.107
3.775AlaLys: 3.775 ± 0.085
6.734AlaLeu: 6.734 ± 0.112
1.94AlaMet: 1.94 ± 0.052
2.214AlaAsn: 2.214 ± 0.061
2.003AlaPro: 2.003 ± 0.052
1.57AlaGln: 1.57 ± 0.055
2.61AlaArg: 2.61 ± 0.071
4.024AlaSer: 4.024 ± 0.082
3.184AlaThr: 3.184 ± 0.088
5.943AlaVal: 5.943 ± 0.102
0.528AlaTrp: 0.528 ± 0.029
2.065AlaTyr: 2.065 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.74CysAla: 0.74 ± 0.034
0.186CysCys: 0.186 ± 0.023
0.602CysAsp: 0.602 ± 0.036
0.741CysGlu: 0.741 ± 0.036
0.457CysPhe: 0.457 ± 0.028
1.469CysGly: 1.469 ± 0.06
0.225CysHis: 0.225 ± 0.019
0.829CysIle: 0.829 ± 0.037
0.691CysLys: 0.691 ± 0.033
0.844CysLeu: 0.844 ± 0.037
0.334CysMet: 0.334 ± 0.019
0.51CysAsn: 0.51 ± 0.028
0.868CysPro: 0.868 ± 0.051
0.343CysGln: 0.343 ± 0.025
0.498CysArg: 0.498 ± 0.031
0.737CysSer: 0.737 ± 0.037
0.617CysThr: 0.617 ± 0.034
0.803CysVal: 0.803 ± 0.036
0.078CysTrp: 0.078 ± 0.01
0.395CysTyr: 0.395 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.84AspAla: 3.84 ± 0.081
0.569AspCys: 0.569 ± 0.032
2.992AspAsp: 2.992 ± 0.069
5.058AspGlu: 5.058 ± 0.104
2.633AspPhe: 2.633 ± 0.057
3.546AspGly: 3.546 ± 0.083
0.992AspHis: 0.992 ± 0.037
4.648AspIle: 4.648 ± 0.105
3.712AspLys: 3.712 ± 0.08
5.56AspLeu: 5.56 ± 0.098
1.854AspMet: 1.854 ± 0.062
2.101AspAsn: 2.101 ± 0.062
2.645AspPro: 2.645 ± 0.068
1.005AspGln: 1.005 ± 0.043
1.938AspArg: 1.938 ± 0.055
3.031AspSer: 3.031 ± 0.062
2.491AspThr: 2.491 ± 0.061
5.261AspVal: 5.261 ± 0.093
0.459AspTrp: 0.459 ± 0.032
2.196AspTyr: 2.196 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
4.616GluAla: 4.616 ± 0.088
0.777GluCys: 0.777 ± 0.037
5.024GluAsp: 5.024 ± 0.107
6.509GluGlu: 6.509 ± 0.127
2.98GluPhe: 2.98 ± 0.071
4.864GluGly: 4.864 ± 0.105
1.217GluHis: 1.217 ± 0.045
6.441GluIle: 6.441 ± 0.113
6.497GluLys: 6.497 ± 0.118
6.785GluLeu: 6.785 ± 0.118
2.165GluMet: 2.165 ± 0.065
4.254GluAsn: 4.254 ± 0.086
2.018GluPro: 2.018 ± 0.056
1.209GluGln: 1.209 ± 0.039
3.197GluArg: 3.197 ± 0.085
4.159GluSer: 4.159 ± 0.078
3.968GluThr: 3.968 ± 0.101
5.437GluVal: 5.437 ± 0.108
0.56GluTrp: 0.56 ± 0.031
2.334GluTyr: 2.334 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
2.491PheAla: 2.491 ± 0.062
0.488PheCys: 0.488 ± 0.023
2.258PheAsp: 2.258 ± 0.066
2.764PheGlu: 2.764 ± 0.079
1.784PhePhe: 1.784 ± 0.07
3.132PheGly: 3.132 ± 0.082
0.758PheHis: 0.758 ± 0.03
3.531PheIle: 3.531 ± 0.088
3.253PheLys: 3.253 ± 0.071
4.37PheLeu: 4.37 ± 0.108
1.288PheMet: 1.288 ± 0.046
2.076PheAsn: 2.076 ± 0.056
1.387PhePro: 1.387 ± 0.055
1.128PheGln: 1.128 ± 0.045
1.506PheArg: 1.506 ± 0.055
2.761PheSer: 2.761 ± 0.076
2.086PheThr: 2.086 ± 0.065
2.836PheVal: 2.836 ± 0.071
0.359PheTrp: 0.359 ± 0.024
1.55PheTyr: 1.55 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
5.001GlyAla: 5.001 ± 0.118
1.004GlyCys: 1.004 ± 0.048
3.923GlyAsp: 3.923 ± 0.076
4.51GlyGlu: 4.51 ± 0.092
3.528GlyPhe: 3.528 ± 0.08
4.929GlyGly: 4.929 ± 0.114
1.25GlyHis: 1.25 ± 0.041
6.476GlyIle: 6.476 ± 0.103
5.152GlyLys: 5.152 ± 0.098
6.234GlyLeu: 6.234 ± 0.108
2.242GlyMet: 2.242 ± 0.066
3.23GlyAsn: 3.23 ± 0.095
1.98GlyPro: 1.98 ± 0.061
1.479GlyGln: 1.479 ± 0.051
2.815GlyArg: 2.815 ± 0.069
4.815GlySer: 4.815 ± 0.105
4.494GlyThr: 4.494 ± 0.117
5.83GlyVal: 5.83 ± 0.102
0.684GlyTrp: 0.684 ± 0.031
2.765GlyTyr: 2.765 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.087HisAla: 1.087 ± 0.04
0.248HisCys: 0.248 ± 0.021
0.986HisAsp: 0.986 ± 0.042
1.528HisGlu: 1.528 ± 0.047
0.709HisPhe: 0.709 ± 0.032
1.485HisGly: 1.485 ± 0.048
0.447HisHis: 0.447 ± 0.028
1.226HisIle: 1.226 ± 0.048
1.085HisLys: 1.085 ± 0.043
1.519HisLeu: 1.519 ± 0.051
0.5HisMet: 0.5 ± 0.03
0.646HisAsn: 0.646 ± 0.032
0.939HisPro: 0.939 ± 0.034
0.38HisGln: 0.38 ± 0.025
0.779HisArg: 0.779 ± 0.034
1.007HisSer: 1.007 ± 0.034
0.796HisThr: 0.796 ± 0.033
1.333HisVal: 1.333 ± 0.046
0.158HisTrp: 0.158 ± 0.017
0.688HisTyr: 0.688 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.134IleAla: 5.134 ± 0.097
0.975IleCys: 0.975 ± 0.037
3.919IleAsp: 3.919 ± 0.089
5.338IleGlu: 5.338 ± 0.108
3.526IlePhe: 3.526 ± 0.099
5.789IleGly: 5.789 ± 0.11
1.51IleHis: 1.51 ± 0.042
6.782IleIle: 6.782 ± 0.125
5.954IleLys: 5.954 ± 0.103
8.169IleLeu: 8.169 ± 0.148
2.192IleMet: 2.192 ± 0.054
3.783IleAsn: 3.783 ± 0.083
3.584IlePro: 3.584 ± 0.08
2.015IleGln: 2.015 ± 0.061
3.063IleArg: 3.063 ± 0.076
5.332IleSer: 5.332 ± 0.095
4.55IleThr: 4.55 ± 0.092
5.566IleVal: 5.566 ± 0.094
0.613IleTrp: 0.613 ± 0.034
2.773IleTyr: 2.773 ± 0.077
0.0IleXaa: 0.0 ± 0.0
Lys
4.367LysAla: 4.367 ± 0.09
0.897LysCys: 0.897 ± 0.041
4.276LysAsp: 4.276 ± 0.083
5.973LysGlu: 5.973 ± 0.126
2.59LysPhe: 2.59 ± 0.063
4.559LysGly: 4.559 ± 0.09
1.279LysHis: 1.279 ± 0.043
6.406LysIle: 6.406 ± 0.123
6.708LysLys: 6.708 ± 0.12
6.236LysLeu: 6.236 ± 0.107
2.199LysMet: 2.199 ± 0.055
4.237LysAsn: 4.237 ± 0.099
2.682LysPro: 2.682 ± 0.072
1.503LysGln: 1.503 ± 0.05
3.333LysArg: 3.333 ± 0.067
4.181LysSer: 4.181 ± 0.096
4.516LysThr: 4.516 ± 0.095
4.883LysVal: 4.883 ± 0.097
0.705LysTrp: 0.705 ± 0.04
2.687LysTyr: 2.687 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
5.762LeuAla: 5.762 ± 0.127
0.99LeuCys: 0.99 ± 0.039
5.063LeuAsp: 5.063 ± 0.092
7.277LeuGlu: 7.277 ± 0.139
3.706LeuPhe: 3.706 ± 0.1
6.506LeuGly: 6.506 ± 0.108
1.42LeuHis: 1.42 ± 0.051
7.371LeuIle: 7.371 ± 0.118
8.295LeuLys: 8.295 ± 0.154
8.064LeuLeu: 8.064 ± 0.145
2.832LeuMet: 2.832 ± 0.07
4.903LeuAsn: 4.903 ± 0.1
3.347LeuPro: 3.347 ± 0.06
2.307LeuGln: 2.307 ± 0.057
3.54LeuArg: 3.54 ± 0.082
5.843LeuSer: 5.843 ± 0.108
4.809LeuThr: 4.809 ± 0.114
6.006LeuVal: 6.006 ± 0.099
0.773LeuTrp: 0.773 ± 0.036
2.633LeuTyr: 2.633 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
2.205MetAla: 2.205 ± 0.062
0.312MetCys: 0.312 ± 0.02
2.181MetAsp: 2.181 ± 0.063
2.556MetGlu: 2.556 ± 0.064
1.031MetPhe: 1.031 ± 0.037
2.537MetGly: 2.537 ± 0.065
0.45MetHis: 0.45 ± 0.026
2.231MetIle: 2.231 ± 0.064
2.462MetLys: 2.462 ± 0.057
2.128MetLeu: 2.128 ± 0.061
0.82MetMet: 0.82 ± 0.039
1.402MetAsn: 1.402 ± 0.039
0.998MetPro: 0.998 ± 0.043
0.681MetGln: 0.681 ± 0.033
1.036MetArg: 1.036 ± 0.034
1.635MetSer: 1.635 ± 0.055
1.25MetThr: 1.25 ± 0.043
2.467MetVal: 2.467 ± 0.062
0.189MetTrp: 0.189 ± 0.016
0.711MetTyr: 0.711 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.864AsnAla: 2.864 ± 0.077
0.613AsnCys: 0.613 ± 0.036
2.205AsnAsp: 2.205 ± 0.062
2.968AsnGlu: 2.968 ± 0.071
2.054AsnPhe: 2.054 ± 0.067
3.372AsnGly: 3.372 ± 0.11
0.839AsnHis: 0.839 ± 0.035
4.044AsnIle: 4.044 ± 0.084
2.933AsnLys: 2.933 ± 0.069
4.749AsnLeu: 4.749 ± 0.097
1.389AsnMet: 1.389 ± 0.048
2.251AsnAsn: 2.251 ± 0.103
2.53AsnPro: 2.53 ± 0.067
1.286AsnGln: 1.286 ± 0.043
1.772AsnArg: 1.772 ± 0.048
3.184AsnSer: 3.184 ± 0.092
2.598AsnThr: 2.598 ± 0.081
3.46AsnVal: 3.46 ± 0.089
0.53AsnTrp: 0.53 ± 0.033
1.917AsnTyr: 1.917 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
2.174ProAla: 2.174 ± 0.068
0.497ProCys: 0.497 ± 0.024
2.307ProAsp: 2.307 ± 0.064
3.932ProGlu: 3.932 ± 0.079
1.624ProPhe: 1.624 ± 0.056
2.574ProGly: 2.574 ± 0.063
0.771ProHis: 0.771 ± 0.039
2.459ProIle: 2.459 ± 0.066
2.325ProLys: 2.325 ± 0.065
3.353ProLeu: 3.353 ± 0.072
0.956ProMet: 0.956 ± 0.035
1.466ProAsn: 1.466 ± 0.054
1.137ProPro: 1.137 ± 0.046
1.12ProGln: 1.12 ± 0.039
1.331ProArg: 1.331 ± 0.05
2.464ProSer: 2.464 ± 0.063
2.0ProThr: 2.0 ± 0.056
3.248ProVal: 3.248 ± 0.072
0.358ProTrp: 0.358 ± 0.025
1.38ProTyr: 1.38 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
1.339GlnAla: 1.339 ± 0.06
0.24GlnCys: 0.24 ± 0.019
1.34GlnAsp: 1.34 ± 0.046
1.703GlnGlu: 1.703 ± 0.056
0.889GlnPhe: 0.889 ± 0.036
1.588GlnGly: 1.588 ± 0.052
0.402GlnHis: 0.402 ± 0.025
1.985GlnIle: 1.985 ± 0.055
2.03GlnLys: 2.03 ± 0.06
1.84GlnLeu: 1.84 ± 0.055
0.789GlnMet: 0.789 ± 0.04
1.439GlnAsn: 1.439 ± 0.059
0.716GlnPro: 0.716 ± 0.032
0.528GlnGln: 0.528 ± 0.032
1.17GlnArg: 1.17 ± 0.046
1.408GlnSer: 1.408 ± 0.052
1.363GlnThr: 1.363 ± 0.057
1.522GlnVal: 1.522 ± 0.049
0.261GlnTrp: 0.261 ± 0.019
0.836GlnTyr: 0.836 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.551ArgAla: 2.551 ± 0.077
0.477ArgCys: 0.477 ± 0.026
2.468ArgAsp: 2.468 ± 0.068
3.455ArgGlu: 3.455 ± 0.076
1.656ArgPhe: 1.656 ± 0.049
2.759ArgGly: 2.759 ± 0.076
0.581ArgHis: 0.581 ± 0.03
3.491ArgIle: 3.491 ± 0.078
3.129ArgLys: 3.129 ± 0.073
2.832ArgLeu: 2.832 ± 0.063
1.209ArgMet: 1.209 ± 0.046
1.802ArgAsn: 1.802 ± 0.049
1.085ArgPro: 1.085 ± 0.044
0.8ArgGln: 0.8 ± 0.033
1.967ArgArg: 1.967 ± 0.068
2.479ArgSer: 2.479 ± 0.06
1.931ArgThr: 1.931 ± 0.049
2.886ArgVal: 2.886 ± 0.072
0.325ArgTrp: 0.325 ± 0.022
1.605ArgTyr: 1.605 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
3.747SerAla: 3.747 ± 0.077
0.694SerCys: 0.694 ± 0.029
3.215SerAsp: 3.215 ± 0.073
4.415SerGlu: 4.415 ± 0.083
2.752SerPhe: 2.752 ± 0.072
4.909SerGly: 4.909 ± 0.102
1.191SerHis: 1.191 ± 0.045
5.016SerIle: 5.016 ± 0.098
4.519SerLys: 4.519 ± 0.094
5.535SerLeu: 5.535 ± 0.096
1.82SerMet: 1.82 ± 0.053
3.07SerAsn: 3.07 ± 0.091
2.39SerPro: 2.39 ± 0.056
1.896SerGln: 1.896 ± 0.052
2.521SerArg: 2.521 ± 0.066
4.619SerSer: 4.619 ± 0.132
3.63SerThr: 3.63 ± 0.091
4.124SerVal: 4.124 ± 0.091
0.62SerTrp: 0.62 ± 0.037
2.406SerTyr: 2.406 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
3.987ThrAla: 3.987 ± 0.1
0.663ThrCys: 0.663 ± 0.036
2.916ThrAsp: 2.916 ± 0.076
3.158ThrGlu: 3.158 ± 0.079
2.165ThrPhe: 2.165 ± 0.058
4.734ThrGly: 4.734 ± 0.109
0.998ThrHis: 0.998 ± 0.039
4.19ThrIle: 4.19 ± 0.095
2.897ThrLys: 2.897 ± 0.075
5.054ThrLeu: 5.054 ± 0.107
1.309ThrMet: 1.309 ± 0.048
2.287ThrAsn: 2.287 ± 0.093
2.379ThrPro: 2.379 ± 0.066
1.28ThrGln: 1.28 ± 0.049
1.996ThrArg: 1.996 ± 0.048
3.781ThrSer: 3.781 ± 0.077
3.245ThrThr: 3.245 ± 0.1
4.716ThrVal: 4.716 ± 0.127
0.472ThrTrp: 0.472 ± 0.028
1.911ThrTyr: 1.911 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
5.19ValAla: 5.19 ± 0.104
0.903ValCys: 0.903 ± 0.042
4.636ValAsp: 4.636 ± 0.084
5.824ValGlu: 5.824 ± 0.113
3.052ValPhe: 3.052 ± 0.074
5.197ValGly: 5.197 ± 0.105
1.241ValHis: 1.241 ± 0.041
5.341ValIle: 5.341 ± 0.091
5.618ValLys: 5.618 ± 0.105
7.261ValLeu: 7.261 ± 0.113
2.162ValMet: 2.162 ± 0.057
3.321ValAsn: 3.321 ± 0.086
3.101ValPro: 3.101 ± 0.068
1.716ValGln: 1.716 ± 0.056
2.565ValArg: 2.565 ± 0.061
4.898ValSer: 4.898 ± 0.091
4.272ValThr: 4.272 ± 0.12
6.394ValVal: 6.394 ± 0.14
0.543ValTrp: 0.543 ± 0.03
2.335ValTyr: 2.335 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.515TrpAla: 0.515 ± 0.03
0.116TrpCys: 0.116 ± 0.011
0.559TrpAsp: 0.559 ± 0.029
0.482TrpGlu: 0.482 ± 0.025
0.385TrpPhe: 0.385 ± 0.027
0.58TrpGly: 0.58 ± 0.031
0.171TrpHis: 0.171 ± 0.017
0.717TrpIle: 0.717 ± 0.037
0.717TrpLys: 0.717 ± 0.037
0.752TrpLeu: 0.752 ± 0.032
0.279TrpMet: 0.279 ± 0.02
0.636TrpAsn: 0.636 ± 0.03
0.21TrpPro: 0.21 ± 0.018
0.229TrpGln: 0.229 ± 0.019
0.374TrpArg: 0.374 ± 0.027
0.465TrpSer: 0.465 ± 0.032
0.497TrpThr: 0.497 ± 0.032
0.568TrpVal: 0.568 ± 0.031
0.139TrpTrp: 0.139 ± 0.016
0.338TrpTyr: 0.338 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.999TyrAla: 1.999 ± 0.052
0.469TyrCys: 0.469 ± 0.029
1.937TyrAsp: 1.937 ± 0.058
2.139TyrGlu: 2.139 ± 0.061
1.546TyrPhe: 1.546 ± 0.057
2.747TyrGly: 2.747 ± 0.071
0.708TyrHis: 0.708 ± 0.033
2.521TyrIle: 2.521 ± 0.065
2.467TyrLys: 2.467 ± 0.074
3.428TyrLeu: 3.428 ± 0.08
1.004TyrMet: 1.004 ± 0.036
1.929TyrAsn: 1.929 ± 0.064
1.476TyrPro: 1.476 ± 0.045
0.912TyrGln: 0.912 ± 0.036
1.478TyrArg: 1.478 ± 0.053
2.269TyrSer: 2.269 ± 0.075
1.84TyrThr: 1.84 ± 0.069
2.287TyrVal: 2.287 ± 0.061
0.371TyrTrp: 0.371 ± 0.023
1.425TyrTyr: 1.425 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2340 proteins (662468 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski