Microvirga sp. 17 mud 1-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Microvirga; unclassified Microvirga

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3992 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3I9U6|A0A2Z3I9U6_9RHIZ SAM-dependent methyltransferase OS=Microvirga sp. 17 mud 1-3 OX=2082949 GN=C4E04_08670 PE=4 SV=1
MM1 pKa = 8.08SEE3 pKa = 3.87EE4 pKa = 3.99HH5 pKa = 6.88AMATIIGGSGRR16 pKa = 11.84QILKK20 pKa = 8.13GTPSTDD26 pKa = 3.22RR27 pKa = 11.84IFGDD31 pKa = 3.17TSGAYY36 pKa = 9.67SFQSGGDD43 pKa = 3.64DD44 pKa = 4.13KK45 pKa = 11.69LYY47 pKa = 10.79GYY49 pKa = 10.33NGSDD53 pKa = 3.15TLYY56 pKa = 11.34GDD58 pKa = 4.2ALRR61 pKa = 11.84LTGTALGGKK70 pKa = 9.47DD71 pKa = 3.81LFYY74 pKa = 11.15GGNGNDD80 pKa = 4.02TIYY83 pKa = 11.44GDD85 pKa = 3.99ALQLRR90 pKa = 11.84DD91 pKa = 3.81YY92 pKa = 10.25ATGGSDD98 pKa = 3.15TLYY101 pKa = 10.09GQSGNDD107 pKa = 3.62HH108 pKa = 7.13LYY110 pKa = 11.18GDD112 pKa = 4.94AKK114 pKa = 10.9YY115 pKa = 10.36LYY117 pKa = 9.75QKK119 pKa = 10.97AHH121 pKa = 6.1GAGDD125 pKa = 3.74RR126 pKa = 11.84LVGGDD131 pKa = 5.23GIDD134 pKa = 3.29TLYY137 pKa = 11.31GDD139 pKa = 4.61GVALFNYY146 pKa = 10.03AVGGNDD152 pKa = 3.09ILSGDD157 pKa = 3.68RR158 pKa = 11.84GKK160 pKa = 9.61DD161 pKa = 3.12TLYY164 pKa = 10.71GDD166 pKa = 4.31SYY168 pKa = 11.01RR169 pKa = 11.84LEE171 pKa = 4.32GRR173 pKa = 11.84SSGGNDD179 pKa = 3.19SLSGGDD185 pKa = 4.38GVDD188 pKa = 4.23FLYY191 pKa = 11.3GDD193 pKa = 4.31AKK195 pKa = 9.71YY196 pKa = 10.23MKK198 pKa = 9.45YY199 pKa = 10.68ASKK202 pKa = 10.86GGNDD206 pKa = 3.47QLIGGSDD213 pKa = 3.78GDD215 pKa = 4.11SLFGDD220 pKa = 4.54GVRR223 pKa = 11.84MTDD226 pKa = 3.21HH227 pKa = 6.85SVGGSDD233 pKa = 3.51TLFGEE238 pKa = 4.54TGDD241 pKa = 3.82DD242 pKa = 3.47ALYY245 pKa = 11.14GDD247 pKa = 5.06AYY249 pKa = 11.28YY250 pKa = 10.11MGSATHH256 pKa = 6.83GGNDD260 pKa = 3.57SLIGADD266 pKa = 3.91GNDD269 pKa = 3.98TIFGDD274 pKa = 3.98AYY276 pKa = 9.83KK277 pKa = 10.17IEE279 pKa = 4.22YY280 pKa = 9.26NGRR283 pKa = 11.84SGADD287 pKa = 3.43TLSGGAGDD295 pKa = 4.17DD296 pKa = 3.68ALYY299 pKa = 11.12GDD301 pKa = 5.17DD302 pKa = 4.46FQDD305 pKa = 3.3GSTTIISSVLGGNDD319 pKa = 3.94RR320 pKa = 11.84IDD322 pKa = 3.64GGTGNDD328 pKa = 3.76FLAGGLGSDD337 pKa = 3.45VFVFHH342 pKa = 6.97KK343 pKa = 10.15WDD345 pKa = 3.54GRR347 pKa = 11.84DD348 pKa = 3.89VIWDD352 pKa = 3.86FCGTASKK359 pKa = 10.34IPQGDD364 pKa = 4.08KK365 pKa = 10.28IDD367 pKa = 3.92LSDD370 pKa = 2.98WHH372 pKa = 6.39FASFDD377 pKa = 3.33QLTPYY382 pKa = 9.66IHH384 pKa = 6.7YY385 pKa = 10.74NSLDD389 pKa = 3.5TGAVTIDD396 pKa = 3.74LPEE399 pKa = 4.3GQTIILLPVGNNGGIQQLTAADD421 pKa = 5.23FILL424 pKa = 5.1

Molecular weight: 44.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3IG99|A0A2Z3IG99_9RHIZ Aminotransferase OS=Microvirga sp. 17 mud 1-3 OX=2082949 GN=C4E04_05440 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38GGRR28 pKa = 11.84KK29 pKa = 9.21VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.11GRR39 pKa = 11.84KK40 pKa = 9.3RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3992

0

3992

1263954

12

2011

316.6

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.145 ± 0.047

0.77 ± 0.012

5.528 ± 0.035

5.871 ± 0.035

3.715 ± 0.027

8.629 ± 0.04

1.985 ± 0.02

5.171 ± 0.028

3.252 ± 0.029

10.401 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.019

2.492 ± 0.023

5.369 ± 0.034

3.074 ± 0.024

7.418 ± 0.046

5.426 ± 0.025

5.315 ± 0.032

7.553 ± 0.031

1.299 ± 0.014

2.225 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski