Amino acid dipepetide frequency for Microvirga sp. 17 mud 1-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.848AlaAla: 15.848 ± 0.149
1.044AlaCys: 1.044 ± 0.033
6.209AlaAsp: 6.209 ± 0.084
7.564AlaGlu: 7.564 ± 0.097
4.667AlaPhe: 4.667 ± 0.077
10.212AlaGly: 10.212 ± 0.116
2.163AlaHis: 2.163 ± 0.039
6.265AlaIle: 6.265 ± 0.073
4.103AlaLys: 4.103 ± 0.065
13.861AlaLeu: 13.861 ± 0.146
3.4AlaMet: 3.4 ± 0.058
2.655AlaAsn: 2.655 ± 0.053
5.598AlaPro: 5.598 ± 0.089
4.122AlaGln: 4.122 ± 0.067
8.583AlaArg: 8.583 ± 0.101
6.386AlaSer: 6.386 ± 0.088
5.727AlaThr: 5.727 ± 0.066
8.844AlaVal: 8.844 ± 0.089
1.517AlaTrp: 1.517 ± 0.037
2.682AlaTyr: 2.682 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.825CysAla: 0.825 ± 0.026
0.079CysCys: 0.079 ± 0.008
0.457CysAsp: 0.457 ± 0.019
0.381CysGlu: 0.381 ± 0.018
0.305CysPhe: 0.305 ± 0.014
0.848CysGly: 0.848 ± 0.025
0.249CysHis: 0.249 ± 0.016
0.398CysIle: 0.398 ± 0.019
0.158CysLys: 0.158 ± 0.01
0.796CysLeu: 0.796 ± 0.028
0.14CysMet: 0.14 ± 0.01
0.208CysAsn: 0.208 ± 0.012
0.43CysPro: 0.43 ± 0.019
0.181CysGln: 0.181 ± 0.013
0.666CysArg: 0.666 ± 0.026
0.403CysSer: 0.403 ± 0.018
0.39CysThr: 0.39 ± 0.021
0.541CysVal: 0.541 ± 0.023
0.086CysTrp: 0.086 ± 0.009
0.157CysTyr: 0.157 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.216AspAla: 6.216 ± 0.073
0.406AspCys: 0.406 ± 0.019
2.786AspAsp: 2.786 ± 0.063
3.778AspGlu: 3.778 ± 0.068
2.12AspPhe: 2.12 ± 0.037
4.973AspGly: 4.973 ± 0.086
1.079AspHis: 1.079 ± 0.034
2.882AspIle: 2.882 ± 0.045
1.731AspLys: 1.731 ± 0.041
6.276AspLeu: 6.276 ± 0.084
1.225AspMet: 1.225 ± 0.029
1.139AspAsn: 1.139 ± 0.035
3.729AspPro: 3.729 ± 0.059
1.584AspGln: 1.584 ± 0.036
4.458AspArg: 4.458 ± 0.068
2.027AspSer: 2.027 ± 0.046
2.528AspThr: 2.528 ± 0.057
4.138AspVal: 4.138 ± 0.061
0.804AspTrp: 0.804 ± 0.026
1.406AspTyr: 1.406 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
8.309GluAla: 8.309 ± 0.094
0.322GluCys: 0.322 ± 0.016
2.772GluAsp: 2.772 ± 0.05
3.519GluGlu: 3.519 ± 0.07
1.656GluPhe: 1.656 ± 0.041
4.738GluGly: 4.738 ± 0.062
1.146GluHis: 1.146 ± 0.032
3.616GluIle: 3.616 ± 0.055
2.255GluLys: 2.255 ± 0.047
4.977GluLeu: 4.977 ± 0.059
1.466GluMet: 1.466 ± 0.034
1.601GluAsn: 1.601 ± 0.032
3.117GluPro: 3.117 ± 0.064
1.938GluGln: 1.938 ± 0.042
5.608GluArg: 5.608 ± 0.088
2.501GluSer: 2.501 ± 0.052
3.703GluThr: 3.703 ± 0.061
3.867GluVal: 3.867 ± 0.068
0.688GluTrp: 0.688 ± 0.024
0.912GluTyr: 0.912 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.328PheAla: 4.328 ± 0.064
0.356PheCys: 0.356 ± 0.017
2.422PheAsp: 2.422 ± 0.048
2.147PheGlu: 2.147 ± 0.043
1.46PhePhe: 1.46 ± 0.043
3.703PheGly: 3.703 ± 0.057
0.71PheHis: 0.71 ± 0.023
1.735PheIle: 1.735 ± 0.039
1.078PheLys: 1.078 ± 0.032
3.544PheLeu: 3.544 ± 0.054
0.825PheMet: 0.825 ± 0.026
1.004PheAsn: 1.004 ± 0.027
1.601PhePro: 1.601 ± 0.041
1.103PheGln: 1.103 ± 0.031
2.419PheArg: 2.419 ± 0.044
2.239PheSer: 2.239 ± 0.039
1.964PheThr: 1.964 ± 0.042
2.979PheVal: 2.979 ± 0.052
0.596PheTrp: 0.596 ± 0.022
0.933PheTyr: 0.933 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
9.065GlyAla: 9.065 ± 0.103
0.794GlyCys: 0.794 ± 0.024
4.186GlyAsp: 4.186 ± 0.07
4.932GlyGlu: 4.932 ± 0.074
3.597GlyPhe: 3.597 ± 0.048
7.369GlyGly: 7.369 ± 0.125
1.909GlyHis: 1.909 ± 0.04
4.859GlyIle: 4.859 ± 0.069
3.015GlyLys: 3.015 ± 0.053
9.449GlyLeu: 9.449 ± 0.094
2.12GlyMet: 2.12 ± 0.042
2.223GlyAsn: 2.223 ± 0.065
3.827GlyPro: 3.827 ± 0.061
2.953GlyGln: 2.953 ± 0.045
6.748GlyArg: 6.748 ± 0.073
4.908GlySer: 4.908 ± 0.072
5.011GlyThr: 5.011 ± 0.086
5.742GlyVal: 5.742 ± 0.065
1.305GlyTrp: 1.305 ± 0.035
2.276GlyTyr: 2.276 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.22HisAla: 2.22 ± 0.047
0.222HisCys: 0.222 ± 0.014
1.209HisAsp: 1.209 ± 0.033
1.102HisGlu: 1.102 ± 0.031
0.782HisPhe: 0.782 ± 0.024
1.887HisGly: 1.887 ± 0.044
0.511HisHis: 0.511 ± 0.022
0.857HisIle: 0.857 ± 0.026
0.493HisLys: 0.493 ± 0.021
2.12HisLeu: 2.12 ± 0.047
0.501HisMet: 0.501 ± 0.019
0.459HisAsn: 0.459 ± 0.02
1.426HisPro: 1.426 ± 0.035
0.544HisGln: 0.544 ± 0.02
1.551HisArg: 1.551 ± 0.033
0.878HisSer: 0.878 ± 0.027
0.81HisThr: 0.81 ± 0.028
1.483HisVal: 1.483 ± 0.036
0.28HisTrp: 0.28 ± 0.014
0.513HisTyr: 0.513 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.135IleAla: 7.135 ± 0.08
0.449IleCys: 0.449 ± 0.021
3.316IleAsp: 3.316 ± 0.05
3.385IleGlu: 3.385 ± 0.049
1.691IlePhe: 1.691 ± 0.036
5.01IleGly: 5.01 ± 0.066
0.938IleHis: 0.938 ± 0.031
2.044IleIle: 2.044 ± 0.045
1.309IleLys: 1.309 ± 0.037
5.241IleLeu: 5.241 ± 0.063
1.045IleMet: 1.045 ± 0.032
1.208IleAsn: 1.208 ± 0.032
2.468IlePro: 2.468 ± 0.047
1.313IleGln: 1.313 ± 0.031
3.544IleArg: 3.544 ± 0.048
2.574IleSer: 2.574 ± 0.05
2.545IleThr: 2.545 ± 0.045
4.775IleVal: 4.775 ± 0.064
0.581IleTrp: 0.581 ± 0.025
1.143IleTyr: 1.143 ± 0.034
0.0IleXaa: 0.0 ± 0.0
Lys
4.564LysAla: 4.564 ± 0.068
0.142LysCys: 0.142 ± 0.011
1.92LysAsp: 1.92 ± 0.051
1.684LysGlu: 1.684 ± 0.038
0.833LysPhe: 0.833 ± 0.03
3.028LysGly: 3.028 ± 0.057
0.563LysHis: 0.563 ± 0.022
1.651LysIle: 1.651 ± 0.042
1.237LysLys: 1.237 ± 0.04
2.991LysLeu: 2.991 ± 0.05
0.666LysMet: 0.666 ± 0.025
0.862LysAsn: 0.862 ± 0.03
2.129LysPro: 2.129 ± 0.04
0.881LysGln: 0.881 ± 0.028
2.366LysArg: 2.366 ± 0.048
1.771LysSer: 1.771 ± 0.042
1.889LysThr: 1.889 ± 0.043
2.499LysVal: 2.499 ± 0.051
0.317LysTrp: 0.317 ± 0.015
0.532LysTyr: 0.532 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.687LeuAla: 13.687 ± 0.137
0.837LeuCys: 0.837 ± 0.028
6.26LeuAsp: 6.26 ± 0.078
5.261LeuGlu: 5.261 ± 0.081
3.727LeuPhe: 3.727 ± 0.068
8.736LeuGly: 8.736 ± 0.101
1.866LeuHis: 1.866 ± 0.041
5.037LeuIle: 5.037 ± 0.068
3.873LeuLys: 3.873 ± 0.06
9.832LeuLeu: 9.832 ± 0.142
2.328LeuMet: 2.328 ± 0.046
2.586LeuAsn: 2.586 ± 0.049
5.662LeuPro: 5.662 ± 0.068
2.858LeuGln: 2.858 ± 0.054
7.278LeuArg: 7.278 ± 0.103
6.809LeuSer: 6.809 ± 0.08
5.772LeuThr: 5.772 ± 0.083
8.227LeuVal: 8.227 ± 0.098
1.236LeuTrp: 1.236 ± 0.031
2.135LeuTyr: 2.135 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.063MetAla: 3.063 ± 0.052
0.127MetCys: 0.127 ± 0.011
1.116MetAsp: 1.116 ± 0.031
1.138MetGlu: 1.138 ± 0.034
0.627MetPhe: 0.627 ± 0.024
1.848MetGly: 1.848 ± 0.041
0.407MetHis: 0.407 ± 0.016
1.234MetIle: 1.234 ± 0.032
0.959MetLys: 0.959 ± 0.034
2.208MetLeu: 2.208 ± 0.038
0.589MetMet: 0.589 ± 0.023
0.747MetAsn: 0.747 ± 0.027
1.464MetPro: 1.464 ± 0.036
0.738MetGln: 0.738 ± 0.024
1.921MetArg: 1.921 ± 0.038
1.537MetSer: 1.537 ± 0.032
1.815MetThr: 1.815 ± 0.038
1.617MetVal: 1.617 ± 0.03
0.18MetTrp: 0.18 ± 0.014
0.29MetTyr: 0.29 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.955AsnAla: 2.955 ± 0.048
0.194AsnCys: 0.194 ± 0.014
1.512AsnAsp: 1.512 ± 0.058
1.227AsnGlu: 1.227 ± 0.033
0.844AsnPhe: 0.844 ± 0.025
2.324AsnGly: 2.324 ± 0.057
0.445AsnHis: 0.445 ± 0.018
1.214AsnIle: 1.214 ± 0.028
0.687AsnLys: 0.687 ± 0.027
2.648AsnLeu: 2.648 ± 0.048
0.55AsnMet: 0.55 ± 0.02
0.647AsnAsn: 0.647 ± 0.027
1.858AsnPro: 1.858 ± 0.036
0.705AsnGln: 0.705 ± 0.024
1.816AsnArg: 1.816 ± 0.034
1.146AsnSer: 1.146 ± 0.033
1.179AsnThr: 1.179 ± 0.031
1.949AsnVal: 1.949 ± 0.042
0.373AsnTrp: 0.373 ± 0.018
0.644AsnTyr: 0.644 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.186ProAla: 6.186 ± 0.087
0.311ProCys: 0.311 ± 0.016
3.616ProAsp: 3.616 ± 0.047
3.922ProGlu: 3.922 ± 0.057
2.143ProPhe: 2.143 ± 0.04
4.634ProGly: 4.634 ± 0.065
1.146ProHis: 1.146 ± 0.031
2.387ProIle: 2.387 ± 0.043
1.779ProLys: 1.779 ± 0.041
4.966ProLeu: 4.966 ± 0.066
1.165ProMet: 1.165 ± 0.032
1.402ProAsn: 1.402 ± 0.038
2.851ProPro: 2.851 ± 0.07
1.794ProGln: 1.794 ± 0.04
3.17ProArg: 3.17 ± 0.057
3.168ProSer: 3.168 ± 0.055
2.62ProThr: 2.62 ± 0.054
4.513ProVal: 4.513 ± 0.059
0.69ProTrp: 0.69 ± 0.025
1.227ProTyr: 1.227 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.313GlnAla: 4.313 ± 0.064
0.169GlnCys: 0.169 ± 0.01
1.665GlnAsp: 1.665 ± 0.039
1.794GlnGlu: 1.794 ± 0.043
0.952GlnPhe: 0.952 ± 0.031
2.552GlnGly: 2.552 ± 0.048
0.586GlnHis: 0.586 ± 0.021
1.77GlnIle: 1.77 ± 0.045
1.094GlnLys: 1.094 ± 0.031
2.547GlnLeu: 2.547 ± 0.046
0.695GlnMet: 0.695 ± 0.025
0.877GlnAsn: 0.877 ± 0.026
1.705GlnPro: 1.705 ± 0.044
1.107GlnGln: 1.107 ± 0.035
2.361GlnArg: 2.361 ± 0.05
1.675GlnSer: 1.675 ± 0.042
1.581GlnThr: 1.581 ± 0.038
2.303GlnVal: 2.303 ± 0.043
0.377GlnTrp: 0.377 ± 0.016
0.613GlnTyr: 0.613 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.948ArgAla: 7.948 ± 0.091
0.521ArgCys: 0.521 ± 0.021
4.099ArgAsp: 4.099 ± 0.066
4.622ArgGlu: 4.622 ± 0.071
3.026ArgPhe: 3.026 ± 0.05
4.908ArgGly: 4.908 ± 0.066
1.808ArgHis: 1.808 ± 0.04
4.504ArgIle: 4.504 ± 0.069
2.276ArgLys: 2.276 ± 0.045
8.542ArgLeu: 8.542 ± 0.086
1.862ArgMet: 1.862 ± 0.04
1.9ArgAsn: 1.9 ± 0.043
3.924ArgPro: 3.924 ± 0.064
2.644ArgGln: 2.644 ± 0.05
6.269ArgArg: 6.269 ± 0.088
3.939ArgSer: 3.939 ± 0.058
3.682ArgThr: 3.682 ± 0.048
4.972ArgVal: 4.972 ± 0.067
1.005ArgTrp: 1.005 ± 0.031
1.73ArgTyr: 1.73 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
5.976SerAla: 5.976 ± 0.07
0.4SerCys: 0.4 ± 0.018
2.828SerAsp: 2.828 ± 0.051
2.873SerGlu: 2.873 ± 0.057
2.333SerPhe: 2.333 ± 0.041
5.478SerGly: 5.478 ± 0.074
1.107SerHis: 1.107 ± 0.028
2.809SerIle: 2.809 ± 0.045
1.548SerLys: 1.548 ± 0.038
6.173SerLeu: 6.173 ± 0.074
1.261SerMet: 1.261 ± 0.03
1.248SerAsn: 1.248 ± 0.037
2.915SerPro: 2.915 ± 0.052
1.562SerGln: 1.562 ± 0.041
3.821SerArg: 3.821 ± 0.055
3.083SerSer: 3.083 ± 0.069
2.702SerThr: 2.702 ± 0.056
4.044SerVal: 4.044 ± 0.059
0.785SerTrp: 0.785 ± 0.028
1.31SerTyr: 1.31 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.846ThrAla: 5.846 ± 0.07
0.388ThrCys: 0.388 ± 0.015
2.748ThrAsp: 2.748 ± 0.053
2.735ThrGlu: 2.735 ± 0.043
2.159ThrPhe: 2.159 ± 0.049
5.143ThrGly: 5.143 ± 0.07
0.991ThrHis: 0.991 ± 0.031
3.006ThrIle: 3.006 ± 0.051
1.589ThrLys: 1.589 ± 0.041
6.13ThrLeu: 6.13 ± 0.083
1.151ThrMet: 1.151 ± 0.028
1.244ThrAsn: 1.244 ± 0.035
3.135ThrPro: 3.135 ± 0.057
1.404ThrGln: 1.404 ± 0.034
3.37ThrArg: 3.37 ± 0.055
2.805ThrSer: 2.805 ± 0.053
2.774ThrThr: 2.774 ± 0.049
4.537ThrVal: 4.537 ± 0.066
0.708ThrTrp: 0.708 ± 0.028
1.284ThrTyr: 1.284 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
9.242ValAla: 9.242 ± 0.104
0.596ValCys: 0.596 ± 0.02
4.033ValAsp: 4.033 ± 0.06
4.805ValGlu: 4.805 ± 0.069
2.855ValPhe: 2.855 ± 0.051
5.744ValGly: 5.744 ± 0.078
1.429ValHis: 1.429 ± 0.038
3.829ValIle: 3.829 ± 0.064
2.339ValLys: 2.339 ± 0.046
7.874ValLeu: 7.874 ± 0.094
1.9ValMet: 1.9 ± 0.039
1.907ValAsn: 1.907 ± 0.044
4.018ValPro: 4.018 ± 0.054
2.154ValGln: 2.154 ± 0.041
5.218ValArg: 5.218 ± 0.077
4.525ValSer: 4.525 ± 0.059
4.581ValThr: 4.581 ± 0.062
6.072ValVal: 6.072 ± 0.083
0.883ValTrp: 0.883 ± 0.032
1.525ValTyr: 1.525 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.173TrpAla: 1.173 ± 0.034
0.131TrpCys: 0.131 ± 0.011
0.675TrpAsp: 0.675 ± 0.027
0.559TrpGlu: 0.559 ± 0.02
0.506TrpPhe: 0.506 ± 0.02
0.885TrpGly: 0.885 ± 0.025
0.338TrpHis: 0.338 ± 0.016
0.742TrpIle: 0.742 ± 0.024
0.4TrpLys: 0.4 ± 0.018
1.604TrpLeu: 1.604 ± 0.041
0.319TrpMet: 0.319 ± 0.014
0.418TrpAsn: 0.418 ± 0.017
0.685TrpPro: 0.685 ± 0.025
0.512TrpGln: 0.512 ± 0.02
1.168TrpArg: 1.168 ± 0.038
0.816TrpSer: 0.816 ± 0.027
0.785TrpThr: 0.785 ± 0.024
0.764TrpVal: 0.764 ± 0.026
0.229TrpTrp: 0.229 ± 0.014
0.28TrpTyr: 0.28 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.552TyrAla: 2.552 ± 0.042
0.211TyrCys: 0.211 ± 0.014
1.454TyrAsp: 1.454 ± 0.034
1.285TyrGlu: 1.285 ± 0.033
0.86TyrPhe: 0.86 ± 0.026
2.267TyrGly: 2.267 ± 0.051
0.459TyrHis: 0.459 ± 0.018
0.874TyrIle: 0.874 ± 0.027
0.6TyrLys: 0.6 ± 0.025
2.227TyrLeu: 2.227 ± 0.048
0.414TyrMet: 0.414 ± 0.019
0.581TyrAsn: 0.581 ± 0.028
1.149TyrPro: 1.149 ± 0.031
0.641TyrGln: 0.641 ± 0.022
1.848TyrArg: 1.848 ± 0.042
1.068TyrSer: 1.068 ± 0.033
1.089TyrThr: 1.089 ± 0.027
1.665TyrVal: 1.665 ± 0.041
0.35TyrTrp: 0.35 ± 0.016
0.655TyrTyr: 0.655 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3992 proteins (1263954 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski