Thiogranum longum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thiogranum

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2795 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1HFB2|A0A4R1HFB2_9GAMM Ankyrin repeat protein OS=Thiogranum longum OX=1537524 GN=DFR30_1308 PE=4 SV=1
MM1 pKa = 7.82HH2 pKa = 7.4SAQCFKK8 pKa = 10.23TKK10 pKa = 10.46KK11 pKa = 9.71RR12 pKa = 11.84ILAAAVLTALSAAAVGPARR31 pKa = 11.84ADD33 pKa = 3.33VQVYY37 pKa = 10.18DD38 pKa = 3.43WTGTFTMINPANVTLVNTDD57 pKa = 2.99VAGNPTKK64 pKa = 10.87GNQTEE69 pKa = 4.34VTGTFTYY76 pKa = 9.38RR77 pKa = 11.84TGSDD81 pKa = 3.32DD82 pKa = 3.74LVNGGGGSGDD92 pKa = 3.27IAIDD96 pKa = 3.3AFNFFGGGPAVAHH109 pKa = 6.99DD110 pKa = 3.92ATFIAIGDD118 pKa = 3.66GHH120 pKa = 6.6GFNGDD125 pKa = 2.87SSGISNGPGDD135 pKa = 4.54LLKK138 pKa = 11.02GDD140 pKa = 5.92LLFDD144 pKa = 3.73WNGTNNIVVEE154 pKa = 4.62IIWNAGGFLTSDD166 pKa = 3.12MGLAGGVGSSVVAGAGAIAPDD187 pKa = 3.75YY188 pKa = 10.68EE189 pKa = 4.61SRR191 pKa = 11.84CVDD194 pKa = 2.91ITFGVCTIALDD205 pKa = 4.12DD206 pKa = 4.27PAAGATEE213 pKa = 5.1WINPADD219 pKa = 3.8GTLVPDD225 pKa = 4.41ASAFGLTTDD234 pKa = 3.61VSGGPILLATVDD246 pKa = 4.24GNPFAQEE253 pKa = 4.18SVDD256 pKa = 3.71PDD258 pKa = 3.94GPGNPIPPVNRR269 pKa = 11.84HH270 pKa = 5.33IDD272 pKa = 3.78GLSGIAMDD280 pKa = 4.54NGPFPGFGANFDD292 pKa = 3.95VRR294 pKa = 11.84TMMLTAFTDD303 pKa = 3.48TTPPVVTLSPNSISINQGDD322 pKa = 4.52AFDD325 pKa = 4.7PLAPPGVTITVVDD338 pKa = 4.3VVDD341 pKa = 3.93GTLDD345 pKa = 3.35VATDD349 pKa = 3.62CSITNGVLTGAAGNYY364 pKa = 6.68TVTYY368 pKa = 9.69NCSDD372 pKa = 3.69LSANANADD380 pKa = 3.58NGSEE384 pKa = 4.1GVLNVQVVAAGAPIITLSGNASVTQEE410 pKa = 4.19CGVAYY415 pKa = 9.2TDD417 pKa = 4.8AGASCTDD424 pKa = 3.34ALLNPITAPADD435 pKa = 3.67FFLDD439 pKa = 4.0TSALNVSTLGVQSVTWSCTDD459 pKa = 2.99TGGGGTTTVDD469 pKa = 2.81RR470 pKa = 11.84VVTVVDD476 pKa = 4.01TSAPVISLNGAASVAIEE493 pKa = 4.25EE494 pKa = 4.64GSVYY498 pKa = 10.51TDD500 pKa = 3.54AGATATDD507 pKa = 3.99GCDD510 pKa = 3.0VGLPALTVVNTVDD523 pKa = 3.18TSVPSTYY530 pKa = 10.68LVTYY534 pKa = 9.86SVSDD538 pKa = 3.45ASGNPATASRR548 pKa = 11.84TVEE551 pKa = 4.13VQPSAPAIVLQGNQTVNLNVGDD573 pKa = 4.07TFDD576 pKa = 4.65PNNPGAVCSDD586 pKa = 3.69AQDD589 pKa = 2.97ATAPVVSVASNVDD602 pKa = 3.18VTQPGTYY609 pKa = 9.17EE610 pKa = 3.93AVYY613 pKa = 10.71SCTDD617 pKa = 2.98TDD619 pKa = 4.03GNTASVTRR627 pKa = 11.84NVIVQGAGFFSAVDD641 pKa = 3.62SNFTMLTPTGDD652 pKa = 3.84FQNGANDD659 pKa = 5.15IVATWDD665 pKa = 3.36GTFNTSVSDD674 pKa = 3.81SNFNMTLATATPTPYY689 pKa = 10.3EE690 pKa = 4.14GSVWTAHH697 pKa = 6.67NIRR700 pKa = 11.84VFGPGSYY707 pKa = 10.05QFDD710 pKa = 3.85VDD712 pKa = 4.07CTPAQIQAGITDD724 pKa = 4.34CPVTDD729 pKa = 3.9LDD731 pKa = 3.97KK732 pKa = 11.52PEE734 pKa = 4.2GRR736 pKa = 11.84VLTLDD741 pKa = 3.36VGPNQLGAHH750 pKa = 6.74ILFDD754 pKa = 3.52WSTTSNIDD762 pKa = 4.59VVNLWNIDD770 pKa = 3.65GAFTSPSGFLGVTGRR785 pKa = 11.84IFNLVSIDD793 pKa = 3.53ANGNNFSGVPMVDD806 pKa = 3.15GPFIGFHH813 pKa = 6.91ANFNLDD819 pKa = 4.0FNPPIQPAAGVRR831 pKa = 11.84APILGAIQNGATRR844 pKa = 11.84RR845 pKa = 11.84AVLVTDD851 pKa = 5.07GIVTVSTDD859 pKa = 2.49IGTTFDD865 pKa = 3.27WSATDD870 pKa = 3.76DD871 pKa = 4.23ALDD874 pKa = 3.99GGATVTTSSLSFDD887 pKa = 4.19PASVAPGFYY896 pKa = 10.23SVGVTIDD903 pKa = 3.5SGLSSEE909 pKa = 4.5ATGSLILEE917 pKa = 4.54VVASVPSVGLDD928 pKa = 3.33DD929 pKa = 4.99SDD931 pKa = 4.51NDD933 pKa = 4.9GIPDD937 pKa = 3.78SVDD940 pKa = 3.14DD941 pKa = 4.37SGPANSLLVDD951 pKa = 4.44AGDD954 pKa = 3.65ATLGSIISSPGTSLSVGNNAYY975 pKa = 9.7IAGIFGGSIPSTDD988 pKa = 3.98LPSDD992 pKa = 3.47SGVEE996 pKa = 4.0EE997 pKa = 4.47SCIGGCFDD1005 pKa = 3.94FQVSGVSPGGSVMVVLPLSEE1025 pKa = 4.48PLPGGSVYY1033 pKa = 10.59RR1034 pKa = 11.84KK1035 pKa = 8.48YY1036 pKa = 10.76NQSAGTWVDD1045 pKa = 3.91FGVGAGNAIASAGKK1059 pKa = 9.73IAGGLCPDD1067 pKa = 4.46EE1068 pKa = 5.68SSASYY1073 pKa = 11.27DD1074 pKa = 3.47DD1075 pKa = 3.67TLGLVAGDD1083 pKa = 3.25EE1084 pKa = 4.8CIRR1087 pKa = 11.84LTIVDD1092 pKa = 4.31GGANDD1097 pKa = 3.81MDD1099 pKa = 4.68SAANPANGIVYY1110 pKa = 10.0DD1111 pKa = 4.15PGGAGVPATPVFASPSTSLGSASGCTVVTAPIDD1144 pKa = 3.42PRR1146 pKa = 11.84QRR1148 pKa = 11.84GDD1150 pKa = 2.44WWLLAMLLGLLGFHH1164 pKa = 6.09AHH1166 pKa = 5.48MRR1168 pKa = 11.84RR1169 pKa = 11.84SRR1171 pKa = 11.84HH1172 pKa = 4.35

Molecular weight: 118.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2PGH9|A0A4V2PGH9_9GAMM ATP synthase subunit b OS=Thiogranum longum OX=1537524 GN=atpF PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.53NGRR28 pKa = 11.84LVIKK32 pKa = 10.35ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2795

0

2795

882141

29

1966

315.6

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.626 ± 0.046

1.021 ± 0.018

6.0 ± 0.037

6.309 ± 0.058

3.654 ± 0.031

7.763 ± 0.045

2.345 ± 0.023

5.427 ± 0.037

3.837 ± 0.045

10.693 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.025

3.29 ± 0.034

4.698 ± 0.033

3.986 ± 0.034

6.603 ± 0.046

5.796 ± 0.031

5.158 ± 0.043

7.325 ± 0.044

1.301 ± 0.02

2.674 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski