Streptococcus phage Javan7
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ALX1|A0A4D6ALX1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan7 OX=2548303 GN=Javan7_0040 PE=4 SV=1
MM1 pKa = 7.89 EE2 pKa = 5.85 LLQYY6 pKa = 10.68 NNKK9 pKa = 9.88 KK10 pKa = 10.05 ISLVDD15 pKa = 3.22 IDD17 pKa = 4.66 DD18 pKa = 5.14 EE19 pKa = 4.43 IWTGTAYY26 pKa = 11.08 YY27 pKa = 10.52 CDD29 pKa = 4.3 ADD31 pKa = 4.11 TNEE34 pKa = 4.15 TPEE37 pKa = 4.28 DD38 pKa = 3.52 VLVVKK43 pKa = 10.12 NEE45 pKa = 3.8 RR46 pKa = 11.84 GYY48 pKa = 9.13 TEE50 pKa = 3.79 ISEE53 pKa = 4.55 SEE55 pKa = 3.94 IKK57 pKa = 10.61 SIEE60 pKa = 4.11 III62 pKa = 3.76
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.061
IPC2_protein 3.999
IPC_protein 3.884
Toseland 3.706
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.63
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.77
Sillero 3.999
Patrickios 3.02
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A4D6ALV4|A0A4D6ALV4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan7 OX=2548303 GN=Javan7_0020 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.53 KK3 pKa = 10.2 LFKK6 pKa = 10.85 KK7 pKa = 10.7 LFVKK11 pKa = 10.26 KK12 pKa = 10.11 NQVIEE17 pKa = 4.42 TRR19 pKa = 11.84 QKK21 pKa = 8.56 WTIEE25 pKa = 3.9 THH27 pKa = 4.43 GWEE30 pKa = 4.24 ANARR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 11.41 DD37 pKa = 4.21 NMIEE41 pKa = 3.97 HH42 pKa = 6.94 NNRR45 pKa = 11.84 GRR47 pKa = 11.84 TCC49 pKa = 3.47
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.172
IPC2_protein 9.443
IPC_protein 9.516
Toseland 10.555
ProMoST 10.028
Dawson 10.628
Bjellqvist 10.218
Wikipedia 10.73
Rodwell 11.213
Grimsley 10.657
Solomon 10.687
Lehninger 10.672
Nozaki 10.526
DTASelect 10.204
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.54
Patrickios 10.994
IPC_peptide 10.687
IPC2_peptide 8.785
IPC2.peptide.svr19 8.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
10912
38
1373
202.1
22.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.424 ± 0.371
0.651 ± 0.106
6.387 ± 0.261
6.69 ± 0.52
4.124 ± 0.272
6.635 ± 0.471
1.595 ± 0.188
7.505 ± 0.284
8.541 ± 0.475
8.0 ± 0.342
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.193
5.957 ± 0.209
2.923 ± 0.18
3.739 ± 0.181
4.005 ± 0.247
6.296 ± 0.368
6.332 ± 0.328
6.131 ± 0.229
1.301 ± 0.123
4.335 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here