Streptomyces sp. HIT-DPA4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9V5W2|A0A6H9V5W2_9ACTN Isoprenylcysteine carboxylmethyltransferase family protein OS=Streptomyces sp. HIT-DPA4 OX=2615112 GN=F7R91_07580 PE=4 SV=1
MM1 pKa = 7.57SVQQEE6 pKa = 3.66AGAAGEE12 pKa = 4.23ALEE15 pKa = 4.16VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.67VKK48 pKa = 10.82GADD51 pKa = 4.57DD52 pKa = 4.9VLLQDD57 pKa = 4.64RR58 pKa = 11.84GATAPVPLPLVMEE71 pKa = 4.49VADD74 pKa = 4.26SAKK77 pKa = 10.01EE78 pKa = 3.94CPGDD82 pKa = 4.18CIHH85 pKa = 6.41VRR87 pKa = 11.84RR88 pKa = 11.84VGDD91 pKa = 3.32KK92 pKa = 10.92VEE94 pKa = 4.25IYY96 pKa = 10.93GPDD99 pKa = 3.32AEE101 pKa = 4.45

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9V7K2|A0A6H9V7K2_9ACTN Sarcosine oxidase subunit beta family protein OS=Streptomyces sp. HIT-DPA4 OX=2615112 GN=F7R91_10250 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7693

0

7693

2529602

18

7141

328.8

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.348 ± 0.044

0.821 ± 0.008

6.017 ± 0.024

5.799 ± 0.024

2.69 ± 0.015

9.317 ± 0.03

2.342 ± 0.016

3.211 ± 0.02

2.234 ± 0.025

10.316 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.743 ± 0.013

1.781 ± 0.015

6.073 ± 0.032

2.882 ± 0.016

8.143 ± 0.031

5.129 ± 0.021

6.133 ± 0.023

8.438 ± 0.029

1.516 ± 0.01

2.068 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski