Amino acid dipepetide frequency for Streptomyces sp. HIT-DPA4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.624AlaAla: 20.624 ± 0.155	1.113AlaCys: 1.113 ± 0.024	8.241AlaAsp: 8.241 ± 0.063	8.869AlaGlu: 8.869 ± 0.085	3.481AlaPhe: 3.481 ± 0.041	12.592AlaGly: 12.592 ± 0.083	2.931AlaHis: 2.931 ± 0.033	3.455AlaIle: 3.455 ± 0.04	2.902AlaLys: 2.902 ± 0.047	14.124AlaLeu: 14.124 ± 0.092	2.452AlaMet: 2.452 ± 0.031	1.911AlaAsn: 1.911 ± 0.031	6.881AlaPro: 6.881 ± 0.074	4.006AlaGln: 4.006 ± 0.044	10.275AlaArg: 10.275 ± 0.08	5.993AlaSer: 5.993 ± 0.053	6.803AlaThr: 6.803 ± 0.052	12.225AlaVal: 12.225 ± 0.081	1.895AlaTrp: 1.895 ± 0.031	2.703AlaTyr: 2.703 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.094CysAla: 1.094 ± 0.022	0.101CysCys: 0.101 ± 0.007	0.484CysAsp: 0.484 ± 0.015	0.421CysGlu: 0.421 ± 0.013	0.22CysPhe: 0.22 ± 0.009	0.987CysGly: 0.987 ± 0.021	0.202CysHis: 0.202 ± 0.01	0.193CysIle: 0.193 ± 0.01	0.125CysLys: 0.125 ± 0.007	0.787CysLeu: 0.787 ± 0.018	0.127CysMet: 0.127 ± 0.007	0.134CysAsn: 0.134 ± 0.008	0.511CysPro: 0.511 ± 0.017	0.187CysGln: 0.187 ± 0.01	0.654CysArg: 0.654 ± 0.019	0.485CysSer: 0.485 ± 0.014	0.556CysThr: 0.556 ± 0.015	0.668CysVal: 0.668 ± 0.016	0.134CysTrp: 0.134 ± 0.007	0.142CysTyr: 0.142 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.546AspAla: 7.546 ± 0.059	0.449AspCys: 0.449 ± 0.014	3.721AspAsp: 3.721 ± 0.045	4.018AspGlu: 4.018 ± 0.042	1.634AspPhe: 1.634 ± 0.027	6.285AspGly: 6.285 ± 0.058	1.426AspHis: 1.426 ± 0.027	2.015AspIle: 2.015 ± 0.03	1.231AspLys: 1.231 ± 0.027	6.249AspLeu: 6.249 ± 0.06	0.834AspMet: 0.834 ± 0.017	0.999AspAsn: 0.999 ± 0.021	4.402AspPro: 4.402 ± 0.05	1.61AspGln: 1.61 ± 0.029	4.964AspArg: 4.964 ± 0.055	2.63AspSer: 2.63 ± 0.035	3.296AspThr: 3.296 ± 0.037	4.732AspVal: 4.732 ± 0.046	1.027AspTrp: 1.027 ± 0.022	1.1AspTyr: 1.1 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.576GluAla: 7.576 ± 0.081	0.405GluCys: 0.405 ± 0.013	2.96GluAsp: 2.96 ± 0.034	3.656GluGlu: 3.656 ± 0.05	1.494GluPhe: 1.494 ± 0.026	4.402GluGly: 4.402 ± 0.046	1.582GluHis: 1.582 ± 0.029	2.274GluIle: 2.274 ± 0.033	1.491GluLys: 1.491 ± 0.029	6.927GluLeu: 6.927 ± 0.062	0.916GluMet: 0.916 ± 0.022	1.095GluAsn: 1.095 ± 0.022	3.472GluPro: 3.472 ± 0.046	2.385GluGln: 2.385 ± 0.036	5.443GluArg: 5.443 ± 0.058	2.617GluSer: 2.617 ± 0.036	2.945GluThr: 2.945 ± 0.039	4.439GluVal: 4.439 ± 0.045	0.792GluTrp: 0.792 ± 0.016	1.125GluTyr: 1.125 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.553PheAla: 3.553 ± 0.042	0.259PheCys: 0.259 ± 0.011	1.919PheAsp: 1.919 ± 0.031	1.45PheGlu: 1.45 ± 0.024	0.844PhePhe: 0.844 ± 0.022	2.948PheGly: 2.948 ± 0.042	0.634PheHis: 0.634 ± 0.016	0.717PheIle: 0.717 ± 0.018	0.518PheLys: 0.518 ± 0.014	2.581PheLeu: 2.581 ± 0.04	0.409PheMet: 0.409 ± 0.014	0.563PheAsn: 0.563 ± 0.014	1.369PhePro: 1.369 ± 0.02	0.669PheGln: 0.669 ± 0.016	1.846PheArg: 1.846 ± 0.028	1.453PheSer: 1.453 ± 0.025	1.96PheThr: 1.96 ± 0.029	2.248PheVal: 2.248 ± 0.03	0.401PheTrp: 0.401 ± 0.013	0.559PheTyr: 0.559 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	10.593GlyAla: 10.593 ± 0.088	0.866GlyCys: 0.866 ± 0.022	5.132GlyAsp: 5.132 ± 0.049	5.137GlyGlu: 5.137 ± 0.05	2.804GlyPhe: 2.804 ± 0.039	8.51GlyGly: 8.51 ± 0.092	2.373GlyHis: 2.373 ± 0.035	3.468GlyIle: 3.468 ± 0.041	2.452GlyLys: 2.452 ± 0.038	9.314GlyLeu: 9.314 ± 0.062	1.989GlyMet: 1.989 ± 0.029	1.736GlyAsn: 1.736 ± 0.029	5.121GlyPro: 5.121 ± 0.055	2.72GlyGln: 2.72 ± 0.036	7.961GlyArg: 7.961 ± 0.064	5.393GlySer: 5.393 ± 0.049	6.381GlyThr: 6.381 ± 0.055	7.356GlyVal: 7.356 ± 0.062	1.705GlyTrp: 1.705 ± 0.028	2.158GlyTyr: 2.158 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.801HisAla: 2.801 ± 0.042	0.223HisCys: 0.223 ± 0.01	1.359HisAsp: 1.359 ± 0.021	1.288HisGlu: 1.288 ± 0.023	0.629HisPhe: 0.629 ± 0.017	2.423HisGly: 2.423 ± 0.034	0.704HisHis: 0.704 ± 0.018	0.742HisIle: 0.742 ± 0.016	0.399HisLys: 0.399 ± 0.013	2.451HisLeu: 2.451 ± 0.031	0.357HisMet: 0.357 ± 0.013	0.385HisAsn: 0.385 ± 0.014	1.808HisPro: 1.808 ± 0.028	0.665HisGln: 0.665 ± 0.017	2.157HisArg: 2.157 ± 0.031	1.069HisSer: 1.069 ± 0.023	1.392HisThr: 1.392 ± 0.025	1.733HisVal: 1.733 ± 0.029	0.357HisTrp: 0.357 ± 0.011	0.48HisTyr: 0.48 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.732IleAla: 4.732 ± 0.047	0.282IleCys: 0.282 ± 0.011	2.327IleAsp: 2.327 ± 0.032	2.012IleGlu: 2.012 ± 0.03	0.711IlePhe: 0.711 ± 0.017	3.534IleGly: 3.534 ± 0.043	0.628IleHis: 0.628 ± 0.017	0.862IleIle: 0.862 ± 0.021	0.772IleLys: 0.772 ± 0.02	2.455IleLeu: 2.455 ± 0.035	0.457IleMet: 0.457 ± 0.014	0.706IleAsn: 0.706 ± 0.018	1.83IlePro: 1.83 ± 0.028	0.741IleGln: 0.741 ± 0.018	2.383IleArg: 2.383 ± 0.033	1.686IleSer: 1.686 ± 0.03	2.311IleThr: 2.311 ± 0.03	2.781IleVal: 2.781 ± 0.04	0.373IleTrp: 0.373 ± 0.013	0.526IleTyr: 0.526 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.066LysAla: 3.066 ± 0.045	0.116LysCys: 0.116 ± 0.007	1.399LysAsp: 1.399 ± 0.031	1.284LysGlu: 1.284 ± 0.026	0.474LysPhe: 0.474 ± 0.013	1.873LysGly: 1.873 ± 0.035	0.434LysHis: 0.434 ± 0.011	0.89LysIle: 0.89 ± 0.02	0.878LysLys: 0.878 ± 0.027	2.077LysLeu: 2.077 ± 0.031	0.373LysMet: 0.373 ± 0.013	0.568LysAsn: 0.568 ± 0.017	1.402LysPro: 1.402 ± 0.03	0.738LysGln: 0.738 ± 0.018	1.504LysArg: 1.504 ± 0.026	1.207LysSer: 1.207 ± 0.023	1.344LysThr: 1.344 ± 0.024	1.959LysVal: 1.959 ± 0.033	0.287LysTrp: 0.287 ± 0.012	0.469LysTyr: 0.469 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	14.918LeuAla: 14.918 ± 0.099	0.877LeuCys: 0.877 ± 0.021	6.642LeuAsp: 6.642 ± 0.058	4.77LeuGlu: 4.77 ± 0.05	2.58LeuPhe: 2.58 ± 0.037	9.086LeuGly: 9.086 ± 0.065	2.378LeuHis: 2.378 ± 0.034	3.302LeuIle: 3.302 ± 0.045	2.147LeuLys: 2.147 ± 0.03	11.283LeuLeu: 11.283 ± 0.071	1.67LeuMet: 1.67 ± 0.032	1.702LeuAsn: 1.702 ± 0.029	6.479LeuPro: 6.479 ± 0.059	2.302LeuGln: 2.302 ± 0.031	8.791LeuArg: 8.791 ± 0.077	5.327LeuSer: 5.327 ± 0.042	6.87LeuThr: 6.87 ± 0.057	8.863LeuVal: 8.863 ± 0.066	1.276LeuTrp: 1.276 ± 0.026	1.893LeuTyr: 1.893 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.27MetAla: 2.27 ± 0.032	0.142MetCys: 0.142 ± 0.008	0.901MetAsp: 0.901 ± 0.022	0.791MetGlu: 0.791 ± 0.016	0.432MetPhe: 0.432 ± 0.013	1.307MetGly: 1.307 ± 0.026	0.372MetHis: 0.372 ± 0.012	0.663MetIle: 0.663 ± 0.017	0.437MetLys: 0.437 ± 0.014	1.683MetLeu: 1.683 ± 0.025	0.305MetMet: 0.305 ± 0.012	0.444MetAsn: 0.444 ± 0.013	1.116MetPro: 1.116 ± 0.024	0.461MetGln: 0.461 ± 0.014	1.439MetArg: 1.439 ± 0.022	1.319MetSer: 1.319 ± 0.023	1.556MetThr: 1.556 ± 0.027	1.263MetVal: 1.263 ± 0.026	0.215MetTrp: 0.215 ± 0.008	0.311MetTyr: 0.311 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.195AsnAla: 2.195 ± 0.029	0.177AsnCys: 0.177 ± 0.008	0.977AsnAsp: 0.977 ± 0.022	0.859AsnGlu: 0.859 ± 0.02	0.473AsnPhe: 0.473 ± 0.015	1.895AsnGly: 1.895 ± 0.03	0.4AsnHis: 0.4 ± 0.013	0.659AsnIle: 0.659 ± 0.017	0.442AsnLys: 0.442 ± 0.016	1.665AsnLeu: 1.665 ± 0.028	0.285AsnMet: 0.285 ± 0.01	0.431AsnAsn: 0.431 ± 0.014	1.388AsnPro: 1.388 ± 0.029	0.543AsnGln: 0.543 ± 0.017	1.326AsnArg: 1.326 ± 0.024	0.936AsnSer: 0.936 ± 0.022	1.102AsnThr: 1.102 ± 0.025	1.364AsnVal: 1.364 ± 0.025	0.304AsnTrp: 0.304 ± 0.011	0.388AsnTyr: 0.388 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.391ProAla: 8.391 ± 0.082	0.353ProCys: 0.353 ± 0.013	4.499ProAsp: 4.499 ± 0.052	4.394ProGlu: 4.394 ± 0.048	1.494ProPhe: 1.494 ± 0.024	6.597ProGly: 6.597 ± 0.071	1.415ProHis: 1.415 ± 0.024	1.247ProIle: 1.247 ± 0.023	1.22ProLys: 1.22 ± 0.027	5.342ProLeu: 5.342 ± 0.048	0.978ProMet: 0.978 ± 0.019	0.906ProAsn: 0.906 ± 0.019	3.692ProPro: 3.692 ± 0.058	1.839ProGln: 1.839 ± 0.032	4.026ProArg: 4.026 ± 0.045	3.323ProSer: 3.323 ± 0.046	3.197ProThr: 3.197 ± 0.043	5.542ProVal: 5.542 ± 0.054	0.872ProTrp: 0.872 ± 0.021	1.408ProTyr: 1.408 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.855GlnAla: 3.855 ± 0.046	0.185GlnCys: 0.185 ± 0.008	1.527GlnAsp: 1.527 ± 0.027	1.58GlnGlu: 1.58 ± 0.026	0.691GlnPhe: 0.691 ± 0.019	2.389GlnGly: 2.389 ± 0.033	0.722GlnHis: 0.722 ± 0.019	1.117GlnIle: 1.117 ± 0.021	0.65GlnLys: 0.65 ± 0.018	3.187GlnLeu: 3.187 ± 0.039	0.509GlnMet: 0.509 ± 0.014	0.52GlnAsn: 0.52 ± 0.017	1.792GlnPro: 1.792 ± 0.035	1.341GlnGln: 1.341 ± 0.029	2.498GlnArg: 2.498 ± 0.03	1.306GlnSer: 1.306 ± 0.021	1.421GlnThr: 1.421 ± 0.026	2.436GlnVal: 2.436 ± 0.029	0.471GlnTrp: 0.471 ± 0.016	0.619GlnTyr: 0.619 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.804ArgAla: 9.804 ± 0.078	0.634ArgCys: 0.634 ± 0.017	4.315ArgAsp: 4.315 ± 0.052	4.796ArgGlu: 4.796 ± 0.046	2.339ArgPhe: 2.339 ± 0.031	5.909ArgGly: 5.909 ± 0.049	2.156ArgHis: 2.156 ± 0.03	3.335ArgIle: 3.335 ± 0.04	1.749ArgLys: 1.749 ± 0.027	9.101ArgLeu: 9.101 ± 0.078	1.762ArgMet: 1.762 ± 0.028	1.405ArgAsn: 1.405 ± 0.026	5.089ArgPro: 5.089 ± 0.06	2.455ArgGln: 2.455 ± 0.034	7.955ArgArg: 7.955 ± 0.082	4.101ArgSer: 4.101 ± 0.038	5.498ArgThr: 5.498 ± 0.046	5.824ArgVal: 5.824 ± 0.055	1.393ArgTrp: 1.393 ± 0.024	1.806ArgTyr: 1.806 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.783SerAla: 6.783 ± 0.058	0.432SerCys: 0.432 ± 0.013	2.782SerAsp: 2.782 ± 0.034	2.503SerGlu: 2.503 ± 0.034	1.5SerPhe: 1.5 ± 0.028	6.011SerGly: 6.011 ± 0.063	1.031SerHis: 1.031 ± 0.02	1.439SerIle: 1.439 ± 0.028	1.076SerLys: 1.076 ± 0.027	4.938SerLeu: 4.938 ± 0.05	1.09SerMet: 1.09 ± 0.019	0.884SerAsn: 0.884 ± 0.022	3.231SerPro: 3.231 ± 0.043	1.302SerGln: 1.302 ± 0.028	3.808SerArg: 3.808 ± 0.04	2.941SerSer: 2.941 ± 0.041	3.071SerThr: 3.071 ± 0.041	4.351SerVal: 4.351 ± 0.043	0.88SerTrp: 0.88 ± 0.02	1.233SerTyr: 1.233 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	8.787ThrAla: 8.787 ± 0.069	0.473ThrCys: 0.473 ± 0.012	3.672ThrAsp: 3.672 ± 0.041	3.354ThrGlu: 3.354 ± 0.039	1.573ThrPhe: 1.573 ± 0.027	6.653ThrGly: 6.653 ± 0.059	1.238ThrHis: 1.238 ± 0.019	1.695ThrIle: 1.695 ± 0.03	1.241ThrLys: 1.241 ± 0.026	5.706ThrLeu: 5.706 ± 0.049	0.955ThrMet: 0.955 ± 0.019	0.977ThrAsn: 0.977 ± 0.02	4.1ThrPro: 4.1 ± 0.048	1.45ThrGln: 1.45 ± 0.023	4.009ThrArg: 4.009 ± 0.043	3.24ThrSer: 3.24 ± 0.038	3.858ThrThr: 3.858 ± 0.052	6.046ThrVal: 6.046 ± 0.056	0.924ThrTrp: 0.924 ± 0.021	1.379ThrTyr: 1.379 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.548ValAla: 10.548 ± 0.073	0.785ValCys: 0.785 ± 0.021	4.928ValAsp: 4.928 ± 0.049	4.734ValGlu: 4.734 ± 0.054	2.389ValPhe: 2.389 ± 0.035	6.443ValGly: 6.443 ± 0.052	2.032ValHis: 2.032 ± 0.032	2.971ValIle: 2.971 ± 0.037	1.815ValLys: 1.815 ± 0.03	9.403ValLeu: 9.403 ± 0.07	1.434ValMet: 1.434 ± 0.024	1.65ValAsn: 1.65 ± 0.031	5.187ValPro: 5.187 ± 0.049	2.183ValGln: 2.183 ± 0.026	7.22ValArg: 7.22 ± 0.059	4.417ValSer: 4.417 ± 0.047	5.589ValThr: 5.589 ± 0.051	7.916ValVal: 7.916 ± 0.074	1.177ValTrp: 1.177 ± 0.023	1.558ValTyr: 1.558 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.638TrpAla: 1.638 ± 0.026	0.159TrpCys: 0.159 ± 0.007	0.824TrpAsp: 0.824 ± 0.018	0.744TrpGlu: 0.744 ± 0.016	0.509TrpPhe: 0.509 ± 0.017	1.05TrpGly: 1.05 ± 0.021	0.393TrpHis: 0.393 ± 0.012	0.562TrpIle: 0.562 ± 0.014	0.374TrpLys: 0.374 ± 0.013	1.81TrpLeu: 1.81 ± 0.027	0.282TrpMet: 0.282 ± 0.01	0.402TrpAsn: 0.402 ± 0.012	0.797TrpPro: 0.797 ± 0.019	0.613TrpGln: 0.613 ± 0.015	1.325TrpArg: 1.325 ± 0.025	0.918TrpSer: 0.918 ± 0.02	1.033TrpThr: 1.033 ± 0.022	0.995TrpVal: 0.995 ± 0.021	0.346TrpTrp: 0.346 ± 0.012	0.383TrpTyr: 0.383 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.701TyrAla: 2.701 ± 0.039	0.183TyrCys: 0.183 ± 0.009	1.558TyrAsp: 1.558 ± 0.031	1.335TyrGlu: 1.335 ± 0.026	0.631TyrPhe: 0.631 ± 0.017	2.277TyrGly: 2.277 ± 0.034	0.374TyrHis: 0.374 ± 0.012	0.504TyrIle: 0.504 ± 0.015	0.421TyrLys: 0.421 ± 0.014	2.073TyrLeu: 2.073 ± 0.032	0.242TyrMet: 0.242 ± 0.01	0.391TyrAsn: 0.391 ± 0.012	1.065TyrPro: 1.065 ± 0.022	0.607TyrGln: 0.607 ± 0.018	1.841TyrArg: 1.841 ± 0.028	0.926TyrSer: 0.926 ± 0.02	1.146TyrThr: 1.146 ± 0.023	1.638TyrVal: 1.638 ± 0.024	0.328TyrTrp: 0.328 ± 0.012	0.438TyrTyr: 0.438 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7693 proteins (2529602 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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