Oleiagrimonas soli
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2676 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099CZG3|A0A099CZG3_9GAMM 50S ribosomal protein L34 OS=Oleiagrimonas soli OX=1543381 GN=rpmH PE=3 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.57 YY3 pKa = 10.61 SLLAAALALGLSGVANADD21 pKa = 3.69 QNSGPDD27 pKa = 3.4 GTITINGKK35 pKa = 10.11 VIAQTCQVDD44 pKa = 3.95 GNGLGTADD52 pKa = 3.38 NKK54 pKa = 10.17 VVNLPDD60 pKa = 3.47 VLTTALASTGDD71 pKa = 3.74 TAGDD75 pKa = 3.38 TSFQISVTGCDD86 pKa = 3.28 ASLSTVQAYY95 pKa = 9.46 FSGGDD100 pKa = 3.49 VNSGNGRR107 pKa = 11.84 LDD109 pKa = 3.29 NSATGAAGNVQVQLLNGSDD128 pKa = 3.53 NPMDD132 pKa = 4.75 LSGADD137 pKa = 3.66 ASAQSSQQVALSGGAATLSYY157 pKa = 9.95 KK158 pKa = 10.16 ARR160 pKa = 11.84 YY161 pKa = 7.82 YY162 pKa = 10.38 ATGASSAGAVKK173 pKa = 9.53 STVDD177 pKa = 3.63 FTLIYY182 pKa = 10.2 QQ183 pKa = 4.1
Molecular weight: 18.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.63
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.948
Rodwell 3.706
Grimsley 3.541
Solomon 3.91
Lehninger 3.859
Nozaki 4.05
DTASelect 4.406
Thurlkill 3.732
EMBOSS 3.935
Sillero 4.012
Patrickios 1.964
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.912
Protein with the highest isoelectric point:
>tr|A0A099CUS4|A0A099CUS4_9GAMM Replication-associated recombination protein A OS=Oleiagrimonas soli OX=1543381 GN=LF63_0108710 PE=3 SV=1
MM1 pKa = 7.45 LNSLAAGRR9 pKa = 11.84 RR10 pKa = 11.84 LALRR14 pKa = 11.84 LVLWQLGVAAMAGFIFLIQGRR35 pKa = 11.84 RR36 pKa = 11.84 AALAALASALAVALGNALAGARR58 pKa = 11.84 AFSPGGAGQALARR71 pKa = 11.84 LILGTLLKK79 pKa = 9.69 WAVVFTGLYY88 pKa = 9.28 VILVRR93 pKa = 11.84 WQLPPAPAIAGLILAVLVNLFALRR117 pKa = 11.84 FKK119 pKa = 10.83 QQ120 pKa = 3.78
Molecular weight: 12.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.377
IPC2_protein 11.023
IPC_protein 12.398
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.149
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2676
0
2676
872925
32
5770
326.2
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.283 ± 0.064
0.875 ± 0.016
6.196 ± 0.039
5.112 ± 0.061
3.453 ± 0.031
8.294 ± 0.064
2.722 ± 0.025
4.26 ± 0.03
2.971 ± 0.041
10.771 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.435 ± 0.021
2.518 ± 0.036
5.075 ± 0.04
3.75 ± 0.031
7.608 ± 0.075
5.22 ± 0.057
5.082 ± 0.046
7.396 ± 0.049
1.526 ± 0.025
2.453 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here