Pedobacter sp. Leaf194

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3909 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5TMQ9|A0A0Q5TMQ9_9SPHI Cyclic nucleotide-binding protein OS=Pedobacter sp. Leaf194 OX=1736297 GN=ASG14_17975 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.47DD21 pKa = 3.38EE22 pKa = 5.13LIDD25 pKa = 3.42YY26 pKa = 7.68GIRR29 pKa = 11.84SGAPVEE35 pKa = 4.53VIEE38 pKa = 4.56NLQALEE44 pKa = 4.69DD45 pKa = 4.28DD46 pKa = 4.28GEE48 pKa = 4.35PYY50 pKa = 9.33EE51 pKa = 4.57TIEE54 pKa = 4.84EE55 pKa = 4.08IWPDD59 pKa = 3.78YY60 pKa = 7.48PTKK63 pKa = 10.86EE64 pKa = 3.82DD65 pKa = 3.75FFFNEE70 pKa = 4.55DD71 pKa = 3.13EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5UBV4|A0A0Q5UBV4_9SPHI Galanin OS=Pedobacter sp. Leaf194 OX=1736297 GN=ASG14_01295 PE=4 SV=1
MM1 pKa = 8.06AIILPQNFFLQPAIKK16 pKa = 9.82IIRR19 pKa = 11.84NAVKK23 pKa = 10.67SNFLIIEE30 pKa = 4.15PAVSILIPRR39 pKa = 11.84ATKK42 pKa = 10.33SAISCRR48 pKa = 11.84INNIISQKK56 pKa = 7.44MEE58 pKa = 4.06RR59 pKa = 11.84LRR61 pKa = 11.84CCVSVEE67 pKa = 3.87LQGFMRR73 pKa = 11.84FRR75 pKa = 11.84YY76 pKa = 9.6LGQCFARR83 pKa = 11.84KK84 pKa = 9.44SSYY87 pKa = 9.88LTVV90 pKa = 3.19

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3909

0

3909

1331561

36

3128

340.6

38.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.608 ± 0.04

0.747 ± 0.012

5.333 ± 0.028

5.759 ± 0.037

5.165 ± 0.033

6.754 ± 0.037

1.693 ± 0.016

7.435 ± 0.039

7.589 ± 0.035

9.495 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.017

5.943 ± 0.041

3.65 ± 0.02

3.5 ± 0.024

3.658 ± 0.025

6.45 ± 0.028

5.627 ± 0.038

6.265 ± 0.029

1.15 ± 0.017

3.962 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski