Amino acid dipepetide frequency for Pedobacter sp. Leaf194

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.377AlaAla: 6.377 ± 0.092	0.641AlaCys: 0.641 ± 0.026	4.645AlaAsp: 4.645 ± 0.062	4.825AlaGlu: 4.825 ± 0.059	3.786AlaPhe: 3.786 ± 0.051	5.617AlaGly: 5.617 ± 0.071	1.192AlaHis: 1.192 ± 0.029	5.581AlaIle: 5.581 ± 0.078	5.411AlaLys: 5.411 ± 0.074	7.189AlaLeu: 7.189 ± 0.088	1.737AlaMet: 1.737 ± 0.042	4.11AlaAsn: 4.11 ± 0.065	2.267AlaPro: 2.267 ± 0.045	2.91AlaGln: 2.91 ± 0.049	2.375AlaArg: 2.375 ± 0.05	4.707AlaSer: 4.707 ± 0.079	4.13AlaThr: 4.13 ± 0.068	4.891AlaVal: 4.891 ± 0.072	0.761AlaTrp: 0.761 ± 0.025	2.932AlaTyr: 2.932 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.531CysAla: 0.531 ± 0.022	0.113CysCys: 0.113 ± 0.01	0.374CysAsp: 0.374 ± 0.016	0.356CysGlu: 0.356 ± 0.015	0.439CysPhe: 0.439 ± 0.018	0.617CysGly: 0.617 ± 0.025	0.18CysHis: 0.18 ± 0.013	0.632CysIle: 0.632 ± 0.022	0.49CysLys: 0.49 ± 0.019	0.747CysLeu: 0.747 ± 0.025	0.162CysMet: 0.162 ± 0.011	0.359CysAsn: 0.359 ± 0.017	0.3CysPro: 0.3 ± 0.018	0.207CysGln: 0.207 ± 0.013	0.294CysArg: 0.294 ± 0.016	0.478CysSer: 0.478 ± 0.02	0.394CysThr: 0.394 ± 0.017	0.45CysVal: 0.45 ± 0.019	0.065CysTrp: 0.065 ± 0.007	0.275CysTyr: 0.275 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.11AspAla: 4.11 ± 0.061	0.375AspCys: 0.375 ± 0.018	2.7AspAsp: 2.7 ± 0.049	3.669AspGlu: 3.669 ± 0.06	3.366AspPhe: 3.366 ± 0.054	3.89AspGly: 3.89 ± 0.058	0.81AspHis: 0.81 ± 0.027	3.82AspIle: 3.82 ± 0.05	4.14AspLys: 4.14 ± 0.064	5.132AspLeu: 5.132 ± 0.062	1.143AspMet: 1.143 ± 0.027	2.719AspAsn: 2.719 ± 0.049	1.766AspPro: 1.766 ± 0.036	1.436AspGln: 1.436 ± 0.034	1.989AspArg: 1.989 ± 0.043	2.91AspSer: 2.91 ± 0.055	2.359AspThr: 2.359 ± 0.044	3.687AspVal: 3.687 ± 0.051	0.786AspTrp: 0.786 ± 0.023	2.519AspTyr: 2.519 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	4.066GluAla: 4.066 ± 0.063	0.3GluCys: 0.3 ± 0.016	2.736GluAsp: 2.736 ± 0.051	3.641GluGlu: 3.641 ± 0.078	2.474GluPhe: 2.474 ± 0.044	3.193GluGly: 3.193 ± 0.063	1.2GluHis: 1.2 ± 0.032	4.85GluIle: 4.85 ± 0.077	5.132GluLys: 5.132 ± 0.069	5.624GluLeu: 5.624 ± 0.08	1.523GluMet: 1.523 ± 0.034	3.772GluAsn: 3.772 ± 0.053	1.604GluPro: 1.604 ± 0.038	2.402GluGln: 2.402 ± 0.041	2.556GluArg: 2.556 ± 0.048	3.078GluSer: 3.078 ± 0.049	3.166GluThr: 3.166 ± 0.058	3.76GluVal: 3.76 ± 0.055	0.617GluTrp: 0.617 ± 0.021	1.893GluTyr: 1.893 ± 0.043	0.001GluXaa: 0.001 ± 0.001
Phe	3.817PheAla: 3.817 ± 0.061	0.479PheCys: 0.479 ± 0.02	3.071PheAsp: 3.071 ± 0.05	2.98PheGlu: 2.98 ± 0.051	2.64PhePhe: 2.64 ± 0.057	3.703PheGly: 3.703 ± 0.065	0.744PheHis: 0.744 ± 0.021	3.771PheIle: 3.771 ± 0.056	3.693PheLys: 3.693 ± 0.06	4.67PheLeu: 4.67 ± 0.077	1.008PheMet: 1.008 ± 0.029	3.176PheAsn: 3.176 ± 0.06	1.719PhePro: 1.719 ± 0.039	1.403PheGln: 1.403 ± 0.03	1.758PheArg: 1.758 ± 0.039	4.042PheSer: 4.042 ± 0.063	3.132PheThr: 3.132 ± 0.053	2.993PheVal: 2.993 ± 0.051	0.617PheTrp: 0.617 ± 0.023	2.239PheTyr: 2.239 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.667GlyAla: 4.667 ± 0.074	0.601GlyCys: 0.601 ± 0.024	3.356GlyAsp: 3.356 ± 0.056	3.432GlyGlu: 3.432 ± 0.058	3.826GlyPhe: 3.826 ± 0.053	4.945GlyGly: 4.945 ± 0.093	1.13GlyHis: 1.13 ± 0.031	5.399GlyIle: 5.399 ± 0.068	5.673GlyLys: 5.673 ± 0.062	6.395GlyLeu: 6.395 ± 0.083	1.634GlyMet: 1.634 ± 0.036	3.906GlyAsn: 3.906 ± 0.06	1.512GlyPro: 1.512 ± 0.038	2.055GlyGln: 2.055 ± 0.045	2.469GlyArg: 2.469 ± 0.045	4.32GlySer: 4.32 ± 0.078	4.091GlyThr: 4.091 ± 0.07	4.247GlyVal: 4.247 ± 0.066	0.926GlyTrp: 0.926 ± 0.028	2.954GlyTyr: 2.954 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.109HisAla: 1.109 ± 0.03	0.168HisCys: 0.168 ± 0.011	0.829HisAsp: 0.829 ± 0.023	0.927HisGlu: 0.927 ± 0.026	1.099HisPhe: 1.099 ± 0.028	1.084HisGly: 1.084 ± 0.031	0.462HisHis: 0.462 ± 0.02	1.273HisIle: 1.273 ± 0.032	1.054HisLys: 1.054 ± 0.032	1.722HisLeu: 1.722 ± 0.036	0.315HisMet: 0.315 ± 0.014	0.888HisAsn: 0.888 ± 0.027	0.93HisPro: 0.93 ± 0.03	0.701HisGln: 0.701 ± 0.023	0.687HisArg: 0.687 ± 0.024	0.949HisSer: 0.949 ± 0.023	0.848HisThr: 0.848 ± 0.027	0.881HisVal: 0.881 ± 0.026	0.228HisTrp: 0.228 ± 0.012	0.778HisTyr: 0.778 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.935IleAla: 5.935 ± 0.074	0.717IleCys: 0.717 ± 0.023	4.329IleAsp: 4.329 ± 0.061	4.504IleGlu: 4.504 ± 0.065	3.546IlePhe: 3.546 ± 0.066	4.83IleGly: 4.83 ± 0.071	1.166IleHis: 1.166 ± 0.034	5.395IleIle: 5.395 ± 0.079	5.555IleLys: 5.555 ± 0.066	6.725IleLeu: 6.725 ± 0.087	1.373IleMet: 1.373 ± 0.033	4.524IleAsn: 4.524 ± 0.059	2.999IlePro: 2.999 ± 0.042	2.263IleGln: 2.263 ± 0.043	2.739IleArg: 2.739 ± 0.043	5.518IleSer: 5.518 ± 0.072	4.367IleThr: 4.367 ± 0.056	4.396IleVal: 4.396 ± 0.061	0.762IleTrp: 0.762 ± 0.027	2.707IleTyr: 2.707 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	5.804LysAla: 5.804 ± 0.078	0.321LysCys: 0.321 ± 0.015	4.181LysAsp: 4.181 ± 0.062	4.526LysGlu: 4.526 ± 0.071	3.168LysPhe: 3.168 ± 0.05	4.687LysGly: 4.687 ± 0.056	1.312LysHis: 1.312 ± 0.03	5.822LysIle: 5.822 ± 0.067	5.904LysLys: 5.904 ± 0.081	6.803LysLeu: 6.803 ± 0.07	1.971LysMet: 1.971 ± 0.041	5.014LysAsn: 5.014 ± 0.061	3.026LysPro: 3.026 ± 0.049	2.816LysGln: 2.816 ± 0.044	2.785LysArg: 2.785 ± 0.051	4.542LysSer: 4.542 ± 0.059	4.678LysThr: 4.678 ± 0.068	4.655LysVal: 4.655 ± 0.063	0.871LysTrp: 0.871 ± 0.028	3.007LysTyr: 3.007 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	7.329LeuAla: 7.329 ± 0.082	0.713LeuCys: 0.713 ± 0.027	4.722LeuAsp: 4.722 ± 0.058	5.021LeuGlu: 5.021 ± 0.081	4.738LeuPhe: 4.738 ± 0.074	5.857LeuGly: 5.857 ± 0.07	1.529LeuHis: 1.529 ± 0.036	6.954LeuIle: 6.954 ± 0.091	7.832LeuLys: 7.832 ± 0.075	9.03LeuLeu: 9.03 ± 0.103	2.142LeuMet: 2.142 ± 0.043	6.078LeuAsn: 6.078 ± 0.066	3.996LeuPro: 3.996 ± 0.057	3.435LeuGln: 3.435 ± 0.051	3.472LeuArg: 3.472 ± 0.045	7.056LeuSer: 7.056 ± 0.09	5.577LeuThr: 5.577 ± 0.069	5.453LeuVal: 5.453 ± 0.067	0.909LeuTrp: 0.909 ± 0.025	3.109LeuTyr: 3.109 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.791MetAla: 1.791 ± 0.04	0.143MetCys: 0.143 ± 0.012	1.142MetAsp: 1.142 ± 0.028	1.311MetGlu: 1.311 ± 0.034	0.89MetPhe: 0.89 ± 0.03	1.443MetGly: 1.443 ± 0.031	0.409MetHis: 0.409 ± 0.018	1.473MetIle: 1.473 ± 0.034	1.983MetLys: 1.983 ± 0.041	2.179MetLeu: 2.179 ± 0.039	0.58MetMet: 0.58 ± 0.026	1.213MetAsn: 1.213 ± 0.033	1.059MetPro: 1.059 ± 0.027	0.979MetGln: 0.979 ± 0.029	0.887MetArg: 0.887 ± 0.026	1.362MetSer: 1.362 ± 0.031	1.006MetThr: 1.006 ± 0.028	1.492MetVal: 1.492 ± 0.035	0.185MetTrp: 0.185 ± 0.012	0.637MetTyr: 0.637 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	4.401AsnAla: 4.401 ± 0.062	0.445AsnCys: 0.445 ± 0.018	2.9AsnAsp: 2.9 ± 0.043	3.192AsnGlu: 3.192 ± 0.054	3.22AsnPhe: 3.22 ± 0.054	4.182AsnGly: 4.182 ± 0.069	1.048AsnHis: 1.048 ± 0.033	4.31AsnIle: 4.31 ± 0.067	3.985AsnLys: 3.985 ± 0.051	5.723AsnLeu: 5.723 ± 0.062	1.231AsnMet: 1.231 ± 0.032	3.48AsnAsn: 3.48 ± 0.066	2.827AsnPro: 2.827 ± 0.048	2.213AsnGln: 2.213 ± 0.042	2.331AsnArg: 2.331 ± 0.046	3.58AsnSer: 3.58 ± 0.066	3.348AsnThr: 3.348 ± 0.061	3.513AsnVal: 3.513 ± 0.062	0.818AsnTrp: 0.818 ± 0.026	2.682AsnTyr: 2.682 ± 0.05	0.001AsnXaa: 0.001 ± 0.001
Pro	3.132ProAla: 3.132 ± 0.061	0.222ProCys: 0.222 ± 0.013	2.321ProAsp: 2.321 ± 0.043	2.782ProGlu: 2.782 ± 0.05	1.896ProPhe: 1.896 ± 0.038	2.537ProGly: 2.537 ± 0.042	0.587ProHis: 0.587 ± 0.023	2.384ProIle: 2.384 ± 0.045	2.547ProLys: 2.547 ± 0.054	3.231ProLeu: 3.231 ± 0.05	0.789ProMet: 0.789 ± 0.028	2.082ProAsn: 2.082 ± 0.04	0.918ProPro: 0.918 ± 0.037	1.271ProGln: 1.271 ± 0.027	1.017ProArg: 1.017 ± 0.027	2.11ProSer: 2.11 ± 0.041	1.902ProThr: 1.902 ± 0.04	2.96ProVal: 2.96 ± 0.06	0.392ProTrp: 0.392 ± 0.019	1.429ProTyr: 1.429 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.635GlnAla: 2.635 ± 0.049	0.17GlnCys: 0.17 ± 0.012	1.616GlnAsp: 1.616 ± 0.033	1.912GlnGlu: 1.912 ± 0.043	1.686GlnPhe: 1.686 ± 0.032	1.954GlnGly: 1.954 ± 0.043	0.669GlnHis: 0.669 ± 0.026	2.569GlnIle: 2.569 ± 0.043	2.729GlnLys: 2.729 ± 0.058	3.426GlnLeu: 3.426 ± 0.056	0.824GlnMet: 0.824 ± 0.029	2.312GlnAsn: 2.312 ± 0.046	1.342GlnPro: 1.342 ± 0.032	1.728GlnGln: 1.728 ± 0.053	1.368GlnArg: 1.368 ± 0.038	2.14GlnSer: 2.14 ± 0.045	2.078GlnThr: 2.078 ± 0.043	2.185GlnVal: 2.185 ± 0.039	0.374GlnTrp: 0.374 ± 0.018	1.286GlnTyr: 1.286 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.33ArgAla: 2.33 ± 0.044	0.228ArgCys: 0.228 ± 0.014	1.825ArgAsp: 1.825 ± 0.038	2.058ArgGlu: 2.058 ± 0.042	2.084ArgPhe: 2.084 ± 0.046	2.085ArgGly: 2.085 ± 0.044	0.582ArgHis: 0.582 ± 0.019	2.984ArgIle: 2.984 ± 0.049	2.972ArgLys: 2.972 ± 0.051	3.691ArgLeu: 3.691 ± 0.053	0.99ArgMet: 0.99 ± 0.026	2.329ArgAsn: 2.329 ± 0.044	1.253ArgPro: 1.253 ± 0.034	1.307ArgGln: 1.307 ± 0.03	1.473ArgArg: 1.473 ± 0.031	2.143ArgSer: 2.143 ± 0.044	1.916ArgThr: 1.916 ± 0.037	2.195ArgVal: 2.195 ± 0.044	0.437ArgTrp: 0.437 ± 0.018	1.698ArgTyr: 1.698 ± 0.038	0.001ArgXaa: 0.001 ± 0.001
Ser	5.084SerAla: 5.084 ± 0.082	0.528SerCys: 0.528 ± 0.02	3.16SerAsp: 3.16 ± 0.054	3.264SerGlu: 3.264 ± 0.051	3.725SerPhe: 3.725 ± 0.059	5.023SerGly: 5.023 ± 0.068	1.014SerHis: 1.014 ± 0.03	4.915SerIle: 4.915 ± 0.062	4.575SerLys: 4.575 ± 0.067	6.204SerLeu: 6.204 ± 0.078	1.32SerMet: 1.32 ± 0.03	3.401SerAsn: 3.401 ± 0.058	2.233SerPro: 2.233 ± 0.042	2.019SerGln: 2.019 ± 0.036	2.345SerArg: 2.345 ± 0.047	4.334SerSer: 4.334 ± 0.081	3.587SerThr: 3.587 ± 0.06	4.316SerVal: 4.316 ± 0.063	0.759SerTrp: 0.759 ± 0.024	2.69SerTyr: 2.69 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.594ThrAla: 4.594 ± 0.065	0.339ThrCys: 0.339 ± 0.016	3.238ThrAsp: 3.238 ± 0.059	3.17ThrGlu: 3.17 ± 0.051	2.827ThrPhe: 2.827 ± 0.048	4.486ThrGly: 4.486 ± 0.068	0.897ThrHis: 0.897 ± 0.03	4.289ThrIle: 4.289 ± 0.058	3.627ThrLys: 3.627 ± 0.055	5.362ThrLeu: 5.362 ± 0.067	1.004ThrMet: 1.004 ± 0.028	3.019ThrAsn: 3.019 ± 0.058	2.43ThrPro: 2.43 ± 0.05	1.842ThrGln: 1.842 ± 0.034	1.775ThrArg: 1.775 ± 0.033	3.518ThrSer: 3.518 ± 0.056	3.283ThrThr: 3.283 ± 0.08	3.671ThrVal: 3.671 ± 0.062	0.649ThrTrp: 0.649 ± 0.018	2.242ThrTyr: 2.242 ± 0.043	0.002ThrXaa: 0.002 ± 0.001
Val	4.756ValAla: 4.756 ± 0.066	0.544ValCys: 0.544 ± 0.022	3.422ValAsp: 3.422 ± 0.05	3.537ValGlu: 3.537 ± 0.057	3.291ValPhe: 3.291 ± 0.055	3.872ValGly: 3.872 ± 0.063	1.0ValHis: 1.0 ± 0.034	4.674ValIle: 4.674 ± 0.063	4.908ValLys: 4.908 ± 0.063	6.055ValLeu: 6.055 ± 0.07	1.367ValMet: 1.367 ± 0.035	3.853ValAsn: 3.853 ± 0.056	2.292ValPro: 2.292 ± 0.044	1.899ValGln: 1.899 ± 0.039	2.09ValArg: 2.09 ± 0.045	4.412ValSer: 4.412 ± 0.069	3.563ValThr: 3.563 ± 0.07	4.125ValVal: 4.125 ± 0.07	0.662ValTrp: 0.662 ± 0.023	2.324ValTyr: 2.324 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.789TrpAla: 0.789 ± 0.027	0.108TrpCys: 0.108 ± 0.01	0.647TrpAsp: 0.647 ± 0.022	0.623TrpGlu: 0.623 ± 0.025	0.627TrpPhe: 0.627 ± 0.024	0.812TrpGly: 0.812 ± 0.026	0.237TrpHis: 0.237 ± 0.014	0.735TrpIle: 0.735 ± 0.021	0.832TrpLys: 0.832 ± 0.027	1.17TrpLeu: 1.17 ± 0.034	0.342TrpMet: 0.342 ± 0.018	0.689TrpAsn: 0.689 ± 0.023	0.372TrpPro: 0.372 ± 0.017	0.485TrpGln: 0.485 ± 0.023	0.47TrpArg: 0.47 ± 0.018	0.665TrpSer: 0.665 ± 0.025	0.587TrpThr: 0.587 ± 0.02	0.677TrpVal: 0.677 ± 0.023	0.196TrpTrp: 0.196 ± 0.012	0.442TrpTyr: 0.442 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.825TyrAla: 2.825 ± 0.048	0.307TyrCys: 0.307 ± 0.016	2.113TyrAsp: 2.113 ± 0.04	1.857TyrGlu: 1.857 ± 0.041	2.327TyrPhe: 2.327 ± 0.043	2.72TyrGly: 2.72 ± 0.052	0.766TyrHis: 0.766 ± 0.025	2.52TyrIle: 2.52 ± 0.044	2.852TyrLys: 2.852 ± 0.053	3.873TyrLeu: 3.873 ± 0.05	0.708TyrMet: 0.708 ± 0.024	2.505TyrAsn: 2.505 ± 0.051	1.66TyrPro: 1.66 ± 0.036	1.633TyrGln: 1.633 ± 0.034	1.699TyrArg: 1.699 ± 0.04	2.631TyrSer: 2.631 ± 0.041	2.255TyrThr: 2.255 ± 0.045	2.1TyrVal: 2.1 ± 0.046	0.492TyrTrp: 0.492 ± 0.019	1.777TyrTyr: 1.777 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3909 proteins (1331561 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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