Beihai sobemo-like virus 27

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KE72|A0A1L3KE72_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 27 OX=1922699 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.44SFAVQSRR9 pKa = 11.84TAHH12 pKa = 5.76IVRR15 pKa = 11.84EE16 pKa = 4.2RR17 pKa = 11.84TISPTDD23 pKa = 3.31EE24 pKa = 4.1EE25 pKa = 4.94LEE27 pKa = 4.7HH28 pKa = 7.91ILYY31 pKa = 9.87HH32 pKa = 6.83AEE34 pKa = 3.83QALAPAKK41 pKa = 9.71WKK43 pKa = 10.77LPSNWNSRR51 pKa = 11.84TAFEE55 pKa = 4.21AALAEE60 pKa = 4.67LDD62 pKa = 3.77WQSSPGYY69 pKa = 8.74PLLRR73 pKa = 11.84EE74 pKa = 4.28APTIGDD80 pKa = 3.37WLLIKK85 pKa = 9.9GTLEE89 pKa = 3.96RR90 pKa = 11.84DD91 pKa = 3.19PSKK94 pKa = 11.26VEE96 pKa = 4.07RR97 pKa = 11.84LWDD100 pKa = 3.57MVQKK104 pKa = 10.28VLKK107 pKa = 10.49GEE109 pKa = 4.49YY110 pKa = 7.59IHH112 pKa = 6.06YY113 pKa = 8.82WRR115 pKa = 11.84VFIKK119 pKa = 10.51SEE121 pKa = 3.49PHH123 pKa = 5.86KK124 pKa = 10.4RR125 pKa = 11.84SKK127 pKa = 10.84ALEE130 pKa = 4.2GRR132 pKa = 11.84WRR134 pKa = 11.84LITAASLPVTVAWMMTFKK152 pKa = 11.02KK153 pKa = 10.83LNDD156 pKa = 3.85RR157 pKa = 11.84LSHH160 pKa = 6.31YY161 pKa = 10.56DD162 pKa = 2.97AALPVQQGYY171 pKa = 7.34VWCSGGWKK179 pKa = 9.58NFKK182 pKa = 10.3RR183 pKa = 11.84RR184 pKa = 11.84IEE186 pKa = 3.97QDD188 pKa = 2.89GLTISVDD195 pKa = 3.04KK196 pKa = 11.04KK197 pKa = 9.77AWDD200 pKa = 3.15WGAPGWAFEE209 pKa = 4.49ADD211 pKa = 3.32LEE213 pKa = 4.58LRR215 pKa = 11.84TRR217 pKa = 11.84LCLNPSPEE225 pKa = 3.73WHH227 pKa = 6.46RR228 pKa = 11.84VATLLYY234 pKa = 10.5ADD236 pKa = 5.05AFQTAKK242 pKa = 10.92LLLPNGIVYY251 pKa = 7.87EE252 pKa = 4.14QQFHH256 pKa = 6.4GFMKK260 pKa = 10.32SGCFNTISTNSNCQILLHH278 pKa = 5.85YY279 pKa = 9.6LAEE282 pKa = 4.22YY283 pKa = 10.04RR284 pKa = 11.84WAKK287 pKa = 10.75AEE289 pKa = 3.91GVSIKK294 pKa = 9.89EE295 pKa = 3.7HH296 pKa = 6.17SKK298 pKa = 10.51ILACGDD304 pKa = 3.37DD305 pKa = 3.86TLQAFYY311 pKa = 10.49TPLYY315 pKa = 10.97GEE317 pKa = 4.9LLEE320 pKa = 4.85QAGCTVKK327 pKa = 10.52EE328 pKa = 4.01AAQNTDD334 pKa = 3.49FMGYY338 pKa = 10.19NYY340 pKa = 10.02DD341 pKa = 3.92GPPEE345 pKa = 3.82PMYY348 pKa = 10.24FAKK351 pKa = 10.65HH352 pKa = 4.96LVKK355 pKa = 10.41FALQKK360 pKa = 9.9PALRR364 pKa = 11.84PEE366 pKa = 4.2TLDD369 pKa = 2.92SYY371 pKa = 10.94MRR373 pKa = 11.84MYY375 pKa = 9.56ATSEE379 pKa = 3.87SRR381 pKa = 11.84HH382 pKa = 4.91FWRR385 pKa = 11.84AVANEE390 pKa = 4.08LEE392 pKa = 4.17IHH394 pKa = 6.35LMSDD398 pKa = 3.21SYY400 pKa = 9.67YY401 pKa = 11.09ARR403 pKa = 11.84WANVPEE409 pKa = 4.24PP410 pKa = 3.84

Molecular weight: 47.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KE72|A0A1L3KE72_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 27 OX=1922699 PE=4 SV=1
MM1 pKa = 7.4EE2 pKa = 4.79FVAKK6 pKa = 10.39ARR8 pKa = 11.84AFLARR13 pKa = 11.84MCLYY17 pKa = 10.22FVGFVVTITNEE28 pKa = 3.72HH29 pKa = 6.07LVALFGAILRR39 pKa = 11.84AVVALFEE46 pKa = 4.29CSMRR50 pKa = 11.84GVHH53 pKa = 6.35HH54 pKa = 6.27MVGALLPEE62 pKa = 4.49ALNPPNRR69 pKa = 11.84KK70 pKa = 9.03NDD72 pKa = 3.65LLRR75 pKa = 11.84QYY77 pKa = 10.13MEE79 pKa = 4.38ANEE82 pKa = 3.99AEE84 pKa = 4.33EE85 pKa = 4.38RR86 pKa = 11.84SRR88 pKa = 11.84SATDD92 pKa = 3.76IIYY95 pKa = 7.62GTSEE99 pKa = 3.69LRR101 pKa = 11.84EE102 pKa = 3.83LHH104 pKa = 6.48MDD106 pKa = 3.97LGYY109 pKa = 10.51QAGFQHH115 pKa = 6.79ASEE118 pKa = 5.12MIISMLALGCLAAWLFSNMPSMNGIGARR146 pKa = 11.84LRR148 pKa = 11.84GCLKK152 pKa = 9.7WRR154 pKa = 11.84PTKK157 pKa = 10.36AVRR160 pKa = 11.84LTDD163 pKa = 3.44VDD165 pKa = 3.74AKK167 pKa = 10.71AAAFATILPEE177 pKa = 3.87TAIPGSNEE185 pKa = 3.27LSMPMTKK192 pKa = 9.94SQVVMFLRR200 pKa = 11.84EE201 pKa = 3.3TDD203 pKa = 3.51AQGKK207 pKa = 8.73NVDD210 pKa = 3.7TVLGNGIRR218 pKa = 11.84FTATRR223 pKa = 11.84LVFPSHH229 pKa = 6.87LGCEE233 pKa = 4.44SFGEE237 pKa = 4.3VWLSSKK243 pKa = 11.0NGGEE247 pKa = 4.08PFCLKK252 pKa = 10.69LGLKK256 pKa = 10.14GDD258 pKa = 3.98YY259 pKa = 10.02QAVAFSNDD267 pKa = 2.88MSFVEE272 pKa = 5.4LPTTVWAGLGVKK284 pKa = 9.55VATIGALSTVGDD296 pKa = 3.75NVTVVGARR304 pKa = 11.84GKK306 pKa = 9.29GTTGVLIAGYY316 pKa = 10.74RR317 pKa = 11.84MGDD320 pKa = 3.41VIYY323 pKa = 11.07NSTTLSGYY331 pKa = 10.33SGAPYY336 pKa = 10.1HH337 pKa = 6.53SGPMTLGVHH346 pKa = 4.82THH348 pKa = 6.44GGKK351 pKa = 10.03QNGGVAATYY360 pKa = 10.1IRR362 pKa = 11.84ARR364 pKa = 11.84LAIYY368 pKa = 9.68DD369 pKa = 4.09ALEE372 pKa = 4.09LDD374 pKa = 3.79IVAEE378 pKa = 4.03SSEE381 pKa = 4.02DD382 pKa = 3.5FMRR385 pKa = 11.84KK386 pKa = 9.11VFDD389 pKa = 3.45SGKK392 pKa = 8.8RR393 pKa = 11.84AHH395 pKa = 6.22RR396 pKa = 11.84TEE398 pKa = 4.33DD399 pKa = 3.13FGDD402 pKa = 3.85FMVVEE407 pKa = 4.6YY408 pKa = 10.39HH409 pKa = 6.59GNHH412 pKa = 5.72HH413 pKa = 5.88TVSKK417 pKa = 10.53QLYY420 pKa = 9.9DD421 pKa = 4.03RR422 pKa = 11.84IKK424 pKa = 9.89SQTYY428 pKa = 9.48HH429 pKa = 7.18PSHH432 pKa = 7.02LSRR435 pKa = 11.84RR436 pKa = 11.84TGLSSSEE443 pKa = 3.68DD444 pKa = 3.3SEE446 pKa = 4.46YY447 pKa = 11.27DD448 pKa = 3.39SEE450 pKa = 5.96SEE452 pKa = 3.95MDD454 pKa = 4.35PPPQLEE460 pKa = 4.22SALNGPVATMAEE472 pKa = 4.3PAVPGYY478 pKa = 10.7DD479 pKa = 3.98DD480 pKa = 5.89GSGLPANIPSSKK492 pKa = 10.09PPSAPPKK499 pKa = 9.44KK500 pKa = 10.01KK501 pKa = 9.97KK502 pKa = 10.04SKK504 pKa = 10.58KK505 pKa = 10.32SGDD508 pKa = 3.57SQRR511 pKa = 11.84ATEE514 pKa = 4.47RR515 pKa = 11.84PTTSRR520 pKa = 11.84KK521 pKa = 9.23SEE523 pKa = 3.95KK524 pKa = 9.28TSSGGSQSRR533 pKa = 11.84KK534 pKa = 9.35SNPSRR539 pKa = 11.84HH540 pKa = 5.77KK541 pKa = 10.48AVKK544 pKa = 9.77RR545 pKa = 11.84DD546 pKa = 2.86IMTKK550 pKa = 10.46FSEE553 pKa = 4.2LTPTHH558 pKa = 7.07RR559 pKa = 11.84ARR561 pKa = 11.84LLTQMAQSLAKK572 pKa = 10.03PP573 pKa = 3.71

Molecular weight: 62.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

983

410

573

491.5

54.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.664 ± 0.223

1.221 ± 0.161

4.374 ± 0.011

6.307 ± 0.672

3.662 ± 0.002

7.121 ± 1.492

3.052 ± 0.079

3.561 ± 0.227

5.697 ± 0.267

9.461 ± 0.683

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.255 ± 0.543

3.255 ± 0.056

5.29 ± 0.05

2.95 ± 0.471

5.799 ± 0.126

7.935 ± 1.222

6.002 ± 0.585

5.697 ± 0.869

2.136 ± 1.337

3.561 ± 0.714
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski