Dishui lake phycodnavirus 1

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9R7H1|A0A2K9R7H1_9PHYC Uncharacterized protein OS=Dishui lake phycodnavirus 1 OX=2079134 GN=DSLPV1_075 PE=4 SV=1
MM1 pKa = 7.1VWSQYY6 pKa = 10.39VYY8 pKa = 10.08EE9 pKa = 4.61ANYY12 pKa = 10.29GLDD15 pKa = 3.49VDD17 pKa = 4.06EE18 pKa = 5.23HH19 pKa = 7.82LSGEE23 pKa = 4.24EE24 pKa = 4.21DD25 pKa = 3.5STDD28 pKa = 3.19PSLNLTVHH36 pKa = 5.98TFWDD40 pKa = 3.86EE41 pKa = 4.2FNDD44 pKa = 3.83EE45 pKa = 4.21LWFLFDD51 pKa = 3.53WLQVLLYY58 pKa = 10.72DD59 pKa = 3.72AVLRR63 pKa = 11.84SKK65 pKa = 9.7VTFDD69 pKa = 4.54DD70 pKa = 5.57FVDD73 pKa = 4.52FCYY76 pKa = 10.91YY77 pKa = 10.74EE78 pKa = 4.35EE79 pKa = 6.0DD80 pKa = 3.71DD81 pKa = 4.09TVLEE85 pKa = 4.01EE86 pKa = 4.88DD87 pKa = 3.72EE88 pKa = 4.38RR89 pKa = 11.84LVYY92 pKa = 10.16IWDD95 pKa = 3.22KK96 pKa = 8.91MQRR99 pKa = 11.84LDD101 pKa = 3.48TTNLMGRR108 pKa = 11.84KK109 pKa = 8.67QFGTFCRR116 pKa = 11.84FINLEE121 pKa = 4.0EE122 pKa = 4.0

Molecular weight: 14.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9R7S8|A0A2K9R7S8_9PHYC Uncharacterized protein OS=Dishui lake phycodnavirus 1 OX=2079134 GN=DSLPV1_218 PE=4 SV=1
MM1 pKa = 7.56TLTEE5 pKa = 4.21WTNLPRR11 pKa = 11.84LQFPIRR17 pKa = 11.84KK18 pKa = 8.58LHH20 pKa = 5.68EE21 pKa = 4.16LFDD24 pKa = 4.24RR25 pKa = 11.84VNTVLDD31 pKa = 3.1HH32 pKa = 6.66RR33 pKa = 11.84LQVRR37 pKa = 11.84KK38 pKa = 9.1VHH40 pKa = 6.14VGAHH44 pKa = 5.73LPKK47 pKa = 10.12HH48 pKa = 4.65VHH50 pKa = 5.71TFLTLSNVAMPSIIGPTMFFKK71 pKa = 10.32MISRR75 pKa = 11.84SEE77 pKa = 4.14SSSPSSISSMSKK89 pKa = 9.95KK90 pKa = 10.14RR91 pKa = 11.84SCVTNFFLDD100 pKa = 3.83APSNAPFFSDD110 pKa = 3.64DD111 pKa = 3.44EE112 pKa = 4.62RR113 pKa = 11.84RR114 pKa = 11.84MSSARR119 pKa = 11.84MPGMSSLVRR128 pKa = 11.84PSDD131 pKa = 3.28KK132 pKa = 9.38YY133 pKa = 7.57TLRR136 pKa = 11.84MRR138 pKa = 11.84STASVIFFASAPVWEE153 pKa = 4.15NRR155 pKa = 11.84SRR157 pKa = 11.84ISSSFIYY164 pKa = 10.42ILTRR168 pKa = 11.84YY169 pKa = 8.08YY170 pKa = 11.02KK171 pKa = 8.08WTARR175 pKa = 11.84SWSSVSASACTSCSTVCVGSTPWRR199 pKa = 11.84IYY201 pKa = 9.38PICTT205 pKa = 3.28

Molecular weight: 23.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

227

0

227

58077

51

2218

255.8

28.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.853 ± 0.455

1.696 ± 0.127

6.126 ± 0.197

6.047 ± 0.248

4.232 ± 0.19

6.231 ± 0.317

2.368 ± 0.14

5.412 ± 0.154

6.436 ± 0.426

8.041 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.121

5.212 ± 0.407

4.074 ± 0.186

3.671 ± 0.173

5.913 ± 0.214

6.758 ± 0.227

6.206 ± 0.202

7.204 ± 0.2

1.155 ± 0.098

3.611 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski