Pontibacter diazotrophicus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8LER3|A0A3D8LER3_9BACT GntP family permease OS=Pontibacter diazotrophicus OX=1400979 GN=DXT99_06925 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.35KK3 pKa = 10.58GEE5 pKa = 4.17RR6 pKa = 11.84NSLRR10 pKa = 11.84MIRR13 pKa = 11.84SILSLFFMAGVAACNGEE30 pKa = 4.35TEE32 pKa = 4.43VVEE35 pKa = 4.28QEE37 pKa = 4.4VVTDD41 pKa = 4.17DD42 pKa = 3.69VAVAEE47 pKa = 4.4TWDD50 pKa = 3.42EE51 pKa = 4.06EE52 pKa = 4.47EE53 pKa = 5.16YY54 pKa = 9.84YY55 pKa = 8.17TTFGTTTYY63 pKa = 11.05YY64 pKa = 10.77EE65 pKa = 4.24DD66 pKa = 3.4WDD68 pKa = 3.67IDD70 pKa = 3.86EE71 pKa = 6.0DD72 pKa = 4.02SLLNEE77 pKa = 4.52EE78 pKa = 4.67EE79 pKa = 4.43FTASFYY85 pKa = 10.8QIWDD89 pKa = 3.65LNNDD93 pKa = 2.98GRR95 pKa = 11.84ISQGEE100 pKa = 4.21WTTVVSDD107 pKa = 3.45YY108 pKa = 11.73GNGIDD113 pKa = 5.04AADD116 pKa = 3.75WQPWDD121 pKa = 3.52TDD123 pKa = 2.95GDD125 pKa = 4.19GFIEE129 pKa = 3.97RR130 pKa = 11.84VEE132 pKa = 3.99FDD134 pKa = 3.24GDD136 pKa = 3.58FAEE139 pKa = 4.82LGWYY143 pKa = 7.47NTWDD147 pKa = 3.58TDD149 pKa = 3.79GDD151 pKa = 4.02GQLTEE156 pKa = 4.4RR157 pKa = 11.84EE158 pKa = 4.52YY159 pKa = 10.16ITGTFTLWDD168 pKa = 3.76TNGDD172 pKa = 3.76NVLDD176 pKa = 3.63TAEE179 pKa = 4.06YY180 pKa = 10.14RR181 pKa = 11.84RR182 pKa = 11.84HH183 pKa = 3.56QTYY186 pKa = 10.42YY187 pKa = 11.31SNN189 pKa = 3.83

Molecular weight: 21.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8L1I2|A0A3D8L1I2_9BACT Uncharacterized protein OS=Pontibacter diazotrophicus OX=1400979 GN=DXT99_25395 PE=4 SV=1
MM1 pKa = 7.69FGRR4 pKa = 11.84INNKK8 pKa = 8.23VPKK11 pKa = 9.71INQQINPSASTEE23 pKa = 4.0SRR25 pKa = 11.84LLARR29 pKa = 11.84GFAKK33 pKa = 9.68MCLLTHH39 pKa = 6.82GSLPLMQHH47 pKa = 5.41YY48 pKa = 9.92VRR50 pKa = 11.84SFRR53 pKa = 11.84TPVLKK58 pKa = 10.24SVRR61 pKa = 11.84QFRR64 pKa = 11.84CLQARR69 pKa = 11.84FLHH72 pKa = 6.61CIPHH76 pKa = 6.44GKK78 pKa = 8.0PACGLLIGFTNLPIRR93 pKa = 11.84DD94 pKa = 3.92MHH96 pKa = 6.75PLDD99 pKa = 3.98NLSIFLLKK107 pKa = 10.86YY108 pKa = 8.66NSCHH112 pKa = 6.86AGHH115 pKa = 6.19TQSAA119 pKa = 3.72

Molecular weight: 13.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5237

0

5237

1775216

32

7403

339.0

37.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.067 ± 0.035

0.73 ± 0.011

5.19 ± 0.028

6.7 ± 0.033

4.529 ± 0.023

7.03 ± 0.037

2.036 ± 0.018

6.001 ± 0.031

5.736 ± 0.037

9.879 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.407 ± 0.018

4.752 ± 0.029

4.161 ± 0.017

4.268 ± 0.028

4.674 ± 0.022

6.056 ± 0.025

5.668 ± 0.034

6.934 ± 0.029

1.193 ± 0.014

3.986 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski