Vulcaniibacterium tengchongense
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4VIU4|A0A3N4VIU4_9GAMM Peptidyl-prolyl cis-trans isomerase OS=Vulcaniibacterium tengchongense OX=1273429 GN=EDC50_2270 PE=3 SV=1
MM1 pKa = 7.49 PFVVTEE7 pKa = 3.85 NCIKK11 pKa = 10.53 CKK13 pKa = 10.0 YY14 pKa = 7.43 TDD16 pKa = 3.85 CVEE19 pKa = 4.15 VCPVDD24 pKa = 4.28 CFHH27 pKa = 7.43 EE28 pKa = 5.34 GPNFLVIDD36 pKa = 4.26 PDD38 pKa = 3.64 EE39 pKa = 6.0 CIDD42 pKa = 3.79 CTLCEE47 pKa = 4.4 PEE49 pKa = 4.54 CPINAIYY56 pKa = 10.45 PEE58 pKa = 4.82 DD59 pKa = 4.37 DD60 pKa = 3.53 VPAGQEE66 pKa = 3.7 AFVALNAEE74 pKa = 4.56 LAKK77 pKa = 10.17 AWPVITARR85 pKa = 11.84 KK86 pKa = 9.92 DD87 pKa = 3.42 PLPDD91 pKa = 3.51 AKK93 pKa = 10.36 EE94 pKa = 4.11 WEE96 pKa = 4.46 GKK98 pKa = 9.09 PGKK101 pKa = 10.15 LDD103 pKa = 3.58 LLEE106 pKa = 4.23 RR107 pKa = 4.71
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.885
IPC2_protein 4.164
IPC_protein 4.088
Toseland 3.897
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.91
Grimsley 3.808
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.329
Thurlkill 3.935
EMBOSS 3.948
Sillero 4.19
Patrickios 1.036
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.09
Protein with the highest isoelectric point:
>tr|A0A3N4V4L9|A0A3N4V4L9_9GAMM Nucleoside-diphosphate-sugar epimerase OS=Vulcaniibacterium tengchongense OX=1273429 GN=EDC50_3103 PE=4 SV=1
MM1 pKa = 5.87 QTAVVVIAVLALGLFLFVRR20 pKa = 11.84 SRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 9.73 RR25 pKa = 11.84 SAAGGGGAGSAGSGGGVNPRR45 pKa = 11.84 PPQRR49 pKa = 11.84 PRR51 pKa = 2.95
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.466
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3153
0
3153
1042108
26
3648
330.5
35.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.77 ± 0.075
0.797 ± 0.014
5.661 ± 0.032
5.712 ± 0.046
3.252 ± 0.027
8.992 ± 0.052
2.123 ± 0.024
3.592 ± 0.029
2.366 ± 0.035
10.93 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.823 ± 0.015
2.153 ± 0.033
5.848 ± 0.046
3.335 ± 0.029
8.923 ± 0.065
4.337 ± 0.036
4.254 ± 0.041
7.201 ± 0.033
1.563 ± 0.02
2.365 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here