Marmoricola mangrovicus
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3882 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L8P1F4|A0A3L8P1F4_9ACTN Imidazoleglycerol-phosphate dehydratase OS=Marmoricola mangrovicus OX=2478913 GN=hisB PE=3 SV=1
MM1 pKa = 7.96 IPEE4 pKa = 4.34 DD5 pKa = 3.41 LKK7 pKa = 10.87 YY8 pKa = 8.99 TAEE11 pKa = 4.42 HH12 pKa = 5.69 EE13 pKa = 4.59 WVRR16 pKa = 11.84 QPGQQEE22 pKa = 3.96 GSVRR26 pKa = 11.84 VGITDD31 pKa = 3.51 YY32 pKa = 11.58 AQDD35 pKa = 3.49 QLGDD39 pKa = 3.4 IVYY42 pKa = 9.88 VQLPEE47 pKa = 4.08 VGTTVAAGDD56 pKa = 3.91 SVGEE60 pKa = 4.1 LEE62 pKa = 4.42 STKK65 pKa = 10.59 SVSEE69 pKa = 3.98 IYY71 pKa = 10.7 APVGGEE77 pKa = 3.7 VVARR81 pKa = 11.84 NEE83 pKa = 4.28 SLDD86 pKa = 3.62 ATPEE90 pKa = 4.13 LVNSEE95 pKa = 4.49 PYY97 pKa = 10.4 AEE99 pKa = 4.2 GWLFEE104 pKa = 4.31 VVPSDD109 pKa = 4.53 SSEE112 pKa = 4.05 LDD114 pKa = 3.26 ALLDD118 pKa = 4.09 DD119 pKa = 4.3 ATYY122 pKa = 11.06 SAGLDD127 pKa = 3.65 GG128 pKa = 5.57
Molecular weight: 13.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 3.681
IPC_protein 3.63
Toseland 3.439
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.528
Rodwell 3.465
Grimsley 3.35
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.884
Thurlkill 3.49
EMBOSS 3.541
Sillero 3.745
Patrickios 1.799
IPC_peptide 3.592
IPC2_peptide 3.732
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|A0A3L8P5Z7|A0A3L8P5Z7_9ACTN Aldehyde dehydrogenase family protein OS=Marmoricola mangrovicus OX=2478913 GN=D9V37_04715 PE=3 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.49 KK7 pKa = 9.45 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.48 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.51 LLKK22 pKa = 8.15 KK23 pKa = 9.12 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3882
0
3882
1253811
29
1617
323.0
34.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.135 ± 0.049
0.735 ± 0.011
6.271 ± 0.034
5.601 ± 0.039
2.758 ± 0.023
9.234 ± 0.029
2.313 ± 0.021
3.117 ± 0.029
1.86 ± 0.028
10.532 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.782 ± 0.016
1.693 ± 0.02
5.467 ± 0.025
2.942 ± 0.019
7.803 ± 0.044
5.397 ± 0.031
5.986 ± 0.032
9.83 ± 0.044
1.532 ± 0.016
2.009 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here