Mycobacterium phage ThulaThula

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; unclassified Pclasvirinae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TJQ1|A0A5J6TJQ1_9CAUD Uncharacterized protein OS=Mycobacterium phage ThulaThula OX=2599880 GN=33 PE=4 SV=1
MM1 pKa = 7.57SNPTNAEE8 pKa = 3.79DD9 pKa = 3.97YY10 pKa = 10.68EE11 pKa = 4.32FAFYY15 pKa = 10.3FSVKK19 pKa = 9.29GVKK22 pKa = 8.34VTGTGDD28 pKa = 3.72PDD30 pKa = 2.83TWYY33 pKa = 10.22PNIQYY38 pKa = 10.8GLRR41 pKa = 11.84GVGDD45 pKa = 4.32APPPLILQAYY55 pKa = 8.48LMNVGLNSDD64 pKa = 3.89PEE66 pKa = 4.46SVRR69 pKa = 11.84FAILYY74 pKa = 6.83TDD76 pKa = 3.83PVSWTVASPEE86 pKa = 3.98EE87 pKa = 4.31VAALPGSVEE96 pKa = 4.55DD97 pKa = 3.61YY98 pKa = 11.17SFAIFGDD105 pKa = 3.97VPADD109 pKa = 3.54EE110 pKa = 4.99NGNAFRR116 pKa = 11.84LGLVGSGEE124 pKa = 4.23YY125 pKa = 10.4GRR127 pKa = 11.84EE128 pKa = 3.94VALLTLPIYY137 pKa = 11.06VDD139 pKa = 3.45TQGQDD144 pKa = 3.28GALHH148 pKa = 6.27NVALCYY154 pKa = 10.24APRR157 pKa = 11.84VSWSAWEE164 pKa = 3.92LDD166 pKa = 3.4AA167 pKa = 7.91

Molecular weight: 18.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TDT7|A0A5J6TDT7_9CAUD Uncharacterized protein OS=Mycobacterium phage ThulaThula OX=2599880 GN=55 PE=4 SV=1
MM1 pKa = 7.31AVIVKK6 pKa = 6.47TTYY9 pKa = 10.07GAPVLYY15 pKa = 10.41RR16 pKa = 11.84NASDD20 pKa = 3.36YY21 pKa = 11.11RR22 pKa = 11.84IDD24 pKa = 3.46HH25 pKa = 6.69RR26 pKa = 11.84GDD28 pKa = 3.16LHH30 pKa = 5.72VVNRR34 pKa = 11.84KK35 pKa = 8.97GRR37 pKa = 11.84IGSIQSGRR45 pKa = 11.84WVQVDD50 pKa = 3.41IAQNRR55 pKa = 11.84DD56 pKa = 2.52ARR58 pKa = 11.84GRR60 pKa = 11.84FTKK63 pKa = 10.52QEE65 pKa = 3.81NN66 pKa = 3.3

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

16376

31

1268

204.7

22.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.256 ± 0.499

1.093 ± 0.187

6.894 ± 0.191

6.033 ± 0.29

2.833 ± 0.194

9.148 ± 0.666

2.174 ± 0.193

4.348 ± 0.132

3.065 ± 0.163

8.042 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.058 ± 0.169

2.894 ± 0.175

6.1 ± 0.185

3.872 ± 0.221

7.065 ± 0.451

4.861 ± 0.209

6.345 ± 0.249

6.748 ± 0.273

1.862 ± 0.132

2.308 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski