Amino acid dipepetide frequency for Mycobacterium phage ThulaThula

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.611AlaAla: 16.611 ± 1.435
1.344AlaCys: 1.344 ± 0.308
8.611AlaAsp: 8.611 ± 0.669
9.038AlaGlu: 9.038 ± 1.012
3.237AlaPhe: 3.237 ± 0.447
9.527AlaGly: 9.527 ± 1.347
2.504AlaHis: 2.504 ± 0.351
5.802AlaIle: 5.802 ± 0.502
4.397AlaLys: 4.397 ± 0.645
9.221AlaLeu: 9.221 ± 0.679
2.87AlaMet: 2.87 ± 0.447
3.115AlaAsn: 3.115 ± 0.409
7.328AlaPro: 7.328 ± 0.875
5.74AlaGln: 5.74 ± 0.649
8.794AlaArg: 8.794 ± 0.847
6.229AlaSer: 6.229 ± 0.659
6.229AlaThr: 6.229 ± 0.698
7.328AlaVal: 7.328 ± 0.583
1.954AlaTrp: 1.954 ± 0.422
2.626AlaTyr: 2.626 ± 0.295
0.0AlaXaa: 0.0 ± 0.0
Cys
0.916CysAla: 0.916 ± 0.215
0.122CysCys: 0.122 ± 0.084
0.916CysAsp: 0.916 ± 0.306
0.794CysGlu: 0.794 ± 0.241
0.122CysPhe: 0.122 ± 0.084
1.221CysGly: 1.221 ± 0.262
0.55CysHis: 0.55 ± 0.163
0.305CysIle: 0.305 ± 0.132
0.244CysLys: 0.244 ± 0.125
0.611CysLeu: 0.611 ± 0.193
0.183CysMet: 0.183 ± 0.1
0.183CysAsn: 0.183 ± 0.107
1.038CysPro: 1.038 ± 0.262
0.427CysGln: 0.427 ± 0.174
1.221CysArg: 1.221 ± 0.283
0.489CysSer: 0.489 ± 0.178
0.672CysThr: 0.672 ± 0.162
0.489CysVal: 0.489 ± 0.213
0.183CysTrp: 0.183 ± 0.094
0.244CysTyr: 0.244 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
9.038AspAla: 9.038 ± 0.921
0.672AspCys: 0.672 ± 0.206
5.985AspAsp: 5.985 ± 0.554
6.168AspGlu: 6.168 ± 0.689
1.649AspPhe: 1.649 ± 0.356
6.595AspGly: 6.595 ± 0.684
1.832AspHis: 1.832 ± 0.345
2.137AspIle: 2.137 ± 0.332
1.405AspLys: 1.405 ± 0.242
5.374AspLeu: 5.374 ± 0.613
1.16AspMet: 1.16 ± 0.276
1.771AspAsn: 1.771 ± 0.358
4.153AspPro: 4.153 ± 0.513
2.565AspGln: 2.565 ± 0.378
4.824AspArg: 4.824 ± 0.515
2.504AspSer: 2.504 ± 0.356
3.603AspThr: 3.603 ± 0.415
4.214AspVal: 4.214 ± 0.459
1.649AspTrp: 1.649 ± 0.289
1.649AspTyr: 1.649 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
7.573GluAla: 7.573 ± 0.735
0.977GluCys: 0.977 ± 0.297
2.687GluAsp: 2.687 ± 0.415
2.87GluGlu: 2.87 ± 0.434
1.954GluPhe: 1.954 ± 0.347
3.969GluGly: 3.969 ± 0.459
1.527GluHis: 1.527 ± 0.312
3.664GluIle: 3.664 ± 0.497
1.649GluLys: 1.649 ± 0.282
7.389GluLeu: 7.389 ± 0.828
1.282GluMet: 1.282 ± 0.218
1.893GluAsn: 1.893 ± 0.356
3.298GluPro: 3.298 ± 0.508
2.565GluGln: 2.565 ± 0.362
3.908GluArg: 3.908 ± 0.611
2.992GluSer: 2.992 ± 0.528
3.42GluThr: 3.42 ± 0.564
4.153GluVal: 4.153 ± 0.538
1.16GluTrp: 1.16 ± 0.225
1.405GluTyr: 1.405 ± 0.268
0.0GluXaa: 0.0 ± 0.0
Phe
3.053PheAla: 3.053 ± 0.478
0.305PheCys: 0.305 ± 0.132
2.382PheAsp: 2.382 ± 0.531
2.076PheGlu: 2.076 ± 0.375
0.427PhePhe: 0.427 ± 0.127
3.908PheGly: 3.908 ± 0.616
0.672PheHis: 0.672 ± 0.217
1.954PheIle: 1.954 ± 0.378
1.221PheLys: 1.221 ± 0.258
1.649PheLeu: 1.649 ± 0.261
0.366PheMet: 0.366 ± 0.131
0.672PheAsn: 0.672 ± 0.155
1.588PhePro: 1.588 ± 0.366
0.916PheGln: 0.916 ± 0.277
1.344PheArg: 1.344 ± 0.228
1.405PheSer: 1.405 ± 0.277
2.015PheThr: 2.015 ± 0.337
1.221PheVal: 1.221 ± 0.241
0.244PheTrp: 0.244 ± 0.117
0.916PheTyr: 0.916 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
9.282GlyAla: 9.282 ± 1.204
0.55GlyCys: 0.55 ± 0.185
5.374GlyAsp: 5.374 ± 0.515
4.824GlyGlu: 4.824 ± 0.572
3.237GlyPhe: 3.237 ± 0.584
10.504GlyGly: 10.504 ± 2.153
1.649GlyHis: 1.649 ± 0.282
2.809GlyIle: 2.809 ± 0.472
2.992GlyLys: 2.992 ± 0.393
7.939GlyLeu: 7.939 ± 0.747
1.954GlyMet: 1.954 ± 0.338
2.931GlyAsn: 2.931 ± 0.542
4.031GlyPro: 4.031 ± 0.541
3.359GlyGln: 3.359 ± 0.608
6.351GlyArg: 6.351 ± 0.642
5.313GlySer: 5.313 ± 0.738
6.29GlyThr: 6.29 ± 0.889
6.84GlyVal: 6.84 ± 0.799
2.076GlyTrp: 2.076 ± 0.523
3.176GlyTyr: 3.176 ± 0.54
0.0GlyXaa: 0.0 ± 0.0
His
2.26HisAla: 2.26 ± 0.297
0.122HisCys: 0.122 ± 0.089
1.71HisAsp: 1.71 ± 0.293
1.099HisGlu: 1.099 ± 0.32
0.366HisPhe: 0.366 ± 0.153
1.405HisGly: 1.405 ± 0.232
0.489HisHis: 0.489 ± 0.224
1.16HisIle: 1.16 ± 0.282
0.977HisLys: 0.977 ± 0.22
1.71HisLeu: 1.71 ± 0.316
0.366HisMet: 0.366 ± 0.135
0.611HisAsn: 0.611 ± 0.201
1.527HisPro: 1.527 ± 0.313
1.038HisGln: 1.038 ± 0.238
1.954HisArg: 1.954 ± 0.446
0.794HisSer: 0.794 ± 0.21
1.588HisThr: 1.588 ± 0.371
1.344HisVal: 1.344 ± 0.309
0.244HisTrp: 0.244 ± 0.13
0.977HisTyr: 0.977 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
5.069IleAla: 5.069 ± 0.395
0.427IleCys: 0.427 ± 0.158
4.092IleAsp: 4.092 ± 0.582
4.031IleGlu: 4.031 ± 0.594
0.733IlePhe: 0.733 ± 0.218
3.725IleGly: 3.725 ± 0.548
0.489IleHis: 0.489 ± 0.161
1.16IleIle: 1.16 ± 0.261
1.344IleLys: 1.344 ± 0.248
1.771IleLeu: 1.771 ± 0.279
0.427IleMet: 0.427 ± 0.2
0.977IleAsn: 0.977 ± 0.245
2.87IlePro: 2.87 ± 0.422
1.954IleGln: 1.954 ± 0.322
3.542IleArg: 3.542 ± 0.34
2.015IleSer: 2.015 ± 0.303
4.336IleThr: 4.336 ± 0.545
3.298IleVal: 3.298 ± 0.455
0.611IleTrp: 0.611 ± 0.178
0.611IleTyr: 0.611 ± 0.225
0.0IleXaa: 0.0 ± 0.0
Lys
3.786LysAla: 3.786 ± 0.528
0.489LysCys: 0.489 ± 0.206
1.282LysAsp: 1.282 ± 0.276
0.916LysGlu: 0.916 ± 0.278
1.405LysPhe: 1.405 ± 0.236
1.466LysGly: 1.466 ± 0.239
0.611LysHis: 0.611 ± 0.248
0.855LysIle: 0.855 ± 0.204
0.855LysLys: 0.855 ± 0.204
2.87LysLeu: 2.87 ± 0.426
0.794LysMet: 0.794 ± 0.191
1.038LysAsn: 1.038 ± 0.267
2.076LysPro: 2.076 ± 0.436
1.221LysGln: 1.221 ± 0.26
2.992LysArg: 2.992 ± 0.49
2.321LysSer: 2.321 ± 0.398
2.382LysThr: 2.382 ± 0.419
1.954LysVal: 1.954 ± 0.324
0.366LysTrp: 0.366 ± 0.134
0.977LysTyr: 0.977 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
11.542LeuAla: 11.542 ± 0.739
0.794LeuCys: 0.794 ± 0.259
6.168LeuAsp: 6.168 ± 0.558
3.725LeuGlu: 3.725 ± 0.371
2.076LeuPhe: 2.076 ± 0.314
8.244LeuGly: 8.244 ± 0.806
1.466LeuHis: 1.466 ± 0.288
4.763LeuIle: 4.763 ± 0.582
1.771LeuLys: 1.771 ± 0.357
7.267LeuLeu: 7.267 ± 0.698
1.649LeuMet: 1.649 ± 0.333
2.137LeuAsn: 2.137 ± 0.421
4.763LeuPro: 4.763 ± 0.548
2.87LeuGln: 2.87 ± 0.395
5.924LeuArg: 5.924 ± 0.649
3.176LeuSer: 3.176 ± 0.44
5.008LeuThr: 5.008 ± 0.584
5.191LeuVal: 5.191 ± 0.501
0.611LeuTrp: 0.611 ± 0.142
1.282LeuTyr: 1.282 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
3.42MetAla: 3.42 ± 0.462
0.305MetCys: 0.305 ± 0.134
0.977MetAsp: 0.977 ± 0.235
0.672MetGlu: 0.672 ± 0.188
0.672MetPhe: 0.672 ± 0.161
1.527MetGly: 1.527 ± 0.301
0.55MetHis: 0.55 ± 0.163
0.55MetIle: 0.55 ± 0.166
0.672MetLys: 0.672 ± 0.184
1.893MetLeu: 1.893 ± 0.332
0.366MetMet: 0.366 ± 0.15
0.733MetAsn: 0.733 ± 0.195
1.344MetPro: 1.344 ± 0.353
0.794MetGln: 0.794 ± 0.215
1.282MetArg: 1.282 ± 0.275
1.832MetSer: 1.832 ± 0.341
2.26MetThr: 2.26 ± 0.472
0.489MetVal: 0.489 ± 0.176
0.183MetTrp: 0.183 ± 0.097
0.061MetTyr: 0.061 ± 0.066
0.0MetXaa: 0.0 ± 0.0
Asn
3.847AsnAla: 3.847 ± 0.453
0.305AsnCys: 0.305 ± 0.159
2.26AsnAsp: 2.26 ± 0.429
1.527AsnGlu: 1.527 ± 0.317
0.366AsnPhe: 0.366 ± 0.138
3.481AsnGly: 3.481 ± 0.348
0.55AsnHis: 0.55 ± 0.177
1.282AsnIle: 1.282 ± 0.281
0.55AsnLys: 0.55 ± 0.193
1.954AsnLeu: 1.954 ± 0.43
0.183AsnMet: 0.183 ± 0.1
0.794AsnAsn: 0.794 ± 0.252
2.26AsnPro: 2.26 ± 0.394
0.733AsnGln: 0.733 ± 0.234
2.26AsnArg: 2.26 ± 0.517
1.893AsnSer: 1.893 ± 0.451
1.221AsnThr: 1.221 ± 0.349
2.015AsnVal: 2.015 ± 0.299
0.916AsnTrp: 0.916 ± 0.175
0.55AsnTyr: 0.55 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
7.511ProAla: 7.511 ± 0.77
0.366ProCys: 0.366 ± 0.144
4.275ProAsp: 4.275 ± 0.491
3.908ProGlu: 3.908 ± 0.551
1.588ProPhe: 1.588 ± 0.324
5.863ProGly: 5.863 ± 0.686
0.916ProHis: 0.916 ± 0.231
2.26ProIle: 2.26 ± 0.365
2.076ProLys: 2.076 ± 0.395
4.275ProLeu: 4.275 ± 0.429
1.588ProMet: 1.588 ± 0.302
2.137ProAsn: 2.137 ± 0.396
4.702ProPro: 4.702 ± 0.696
2.504ProGln: 2.504 ± 0.426
4.275ProArg: 4.275 ± 0.61
2.809ProSer: 2.809 ± 0.402
3.176ProThr: 3.176 ± 0.472
4.458ProVal: 4.458 ± 0.437
1.405ProTrp: 1.405 ± 0.288
0.916ProTyr: 0.916 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
4.641GlnAla: 4.641 ± 0.566
0.427GlnCys: 0.427 ± 0.136
1.344GlnAsp: 1.344 ± 0.324
1.588GlnGlu: 1.588 ± 0.326
1.954GlnPhe: 1.954 ± 0.522
3.053GlnGly: 3.053 ± 0.48
0.794GlnHis: 0.794 ± 0.215
2.076GlnIle: 2.076 ± 0.289
1.16GlnLys: 1.16 ± 0.232
3.481GlnLeu: 3.481 ± 0.537
1.405GlnMet: 1.405 ± 0.308
0.733GlnAsn: 0.733 ± 0.22
2.565GlnPro: 2.565 ± 0.381
2.015GlnGln: 2.015 ± 0.345
3.237GlnArg: 3.237 ± 0.349
1.832GlnSer: 1.832 ± 0.337
2.076GlnThr: 2.076 ± 0.339
2.809GlnVal: 2.809 ± 0.424
0.916GlnTrp: 0.916 ± 0.241
0.611GlnTyr: 0.611 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
7.878ArgAla: 7.878 ± 0.724
1.16ArgCys: 1.16 ± 0.29
5.74ArgAsp: 5.74 ± 0.621
3.481ArgGlu: 3.481 ± 0.448
2.015ArgPhe: 2.015 ± 0.255
5.618ArgGly: 5.618 ± 0.671
2.198ArgHis: 2.198 ± 0.393
2.931ArgIle: 2.931 ± 0.396
2.809ArgLys: 2.809 ± 0.364
5.74ArgLeu: 5.74 ± 0.659
2.382ArgMet: 2.382 ± 0.352
2.076ArgAsn: 2.076 ± 0.487
3.664ArgPro: 3.664 ± 0.437
2.137ArgGln: 2.137 ± 0.308
5.863ArgArg: 5.863 ± 0.848
2.87ArgSer: 2.87 ± 0.388
4.519ArgThr: 4.519 ± 0.458
3.908ArgVal: 3.908 ± 0.567
1.405ArgTrp: 1.405 ± 0.306
2.26ArgTyr: 2.26 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.985SerAla: 5.985 ± 0.837
0.244SerCys: 0.244 ± 0.113
3.359SerAsp: 3.359 ± 0.349
2.382SerGlu: 2.382 ± 0.375
1.405SerPhe: 1.405 ± 0.284
5.863SerGly: 5.863 ± 0.919
1.099SerHis: 1.099 ± 0.265
2.137SerIle: 2.137 ± 0.351
1.71SerLys: 1.71 ± 0.292
3.42SerLeu: 3.42 ± 0.352
0.855SerMet: 0.855 ± 0.228
1.221SerAsn: 1.221 ± 0.297
3.237SerPro: 3.237 ± 0.44
1.344SerGln: 1.344 ± 0.254
2.931SerArg: 2.931 ± 0.386
2.26SerSer: 2.26 ± 0.386
2.992SerThr: 2.992 ± 0.447
3.786SerVal: 3.786 ± 0.411
0.977SerTrp: 0.977 ± 0.275
1.405SerTyr: 1.405 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
7.573ThrAla: 7.573 ± 0.662
0.55ThrCys: 0.55 ± 0.207
4.336ThrAsp: 4.336 ± 0.516
4.275ThrGlu: 4.275 ± 0.536
1.527ThrPhe: 1.527 ± 0.447
6.29ThrGly: 6.29 ± 0.831
1.344ThrHis: 1.344 ± 0.23
3.053ThrIle: 3.053 ± 0.479
2.321ThrLys: 2.321 ± 0.32
5.13ThrLeu: 5.13 ± 0.507
0.916ThrMet: 0.916 ± 0.218
1.832ThrAsn: 1.832 ± 0.262
4.214ThrPro: 4.214 ± 0.432
2.198ThrGln: 2.198 ± 0.287
3.237ThrArg: 3.237 ± 0.453
2.015ThrSer: 2.015 ± 0.391
4.458ThrThr: 4.458 ± 0.596
5.557ThrVal: 5.557 ± 0.699
1.466ThrTrp: 1.466 ± 0.306
1.16ThrTyr: 1.16 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
7.939ValAla: 7.939 ± 0.566
0.977ValCys: 0.977 ± 0.27
5.069ValAsp: 5.069 ± 0.656
4.824ValGlu: 4.824 ± 0.55
2.015ValPhe: 2.015 ± 0.29
5.252ValGly: 5.252 ± 0.579
1.588ValHis: 1.588 ± 0.294
2.931ValIle: 2.931 ± 0.412
1.71ValLys: 1.71 ± 0.357
5.191ValLeu: 5.191 ± 0.574
0.977ValMet: 0.977 ± 0.261
2.504ValAsn: 2.504 ± 0.416
3.603ValPro: 3.603 ± 0.372
2.504ValGln: 2.504 ± 0.357
4.214ValArg: 4.214 ± 0.509
3.053ValSer: 3.053 ± 0.434
4.641ValThr: 4.641 ± 0.585
5.679ValVal: 5.679 ± 0.724
1.282ValTrp: 1.282 ± 0.3
1.527ValTyr: 1.527 ± 0.317
0.0ValXaa: 0.0 ± 0.0
Trp
1.771TrpAla: 1.771 ± 0.287
0.55TrpCys: 0.55 ± 0.159
0.916TrpAsp: 0.916 ± 0.245
0.794TrpGlu: 0.794 ± 0.204
1.038TrpPhe: 1.038 ± 0.261
1.405TrpGly: 1.405 ± 0.238
0.427TrpHis: 0.427 ± 0.137
0.611TrpIle: 0.611 ± 0.243
0.427TrpLys: 0.427 ± 0.147
1.71TrpLeu: 1.71 ± 0.293
0.489TrpMet: 0.489 ± 0.155
0.672TrpAsn: 0.672 ± 0.154
1.282TrpPro: 1.282 ± 0.345
1.038TrpGln: 1.038 ± 0.235
0.977TrpArg: 0.977 ± 0.276
1.282TrpSer: 1.282 ± 0.263
0.794TrpThr: 0.794 ± 0.206
1.344TrpVal: 1.344 ± 0.336
0.672TrpTrp: 0.672 ± 0.177
0.427TrpTyr: 0.427 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.87TyrAla: 2.87 ± 0.395
0.244TyrCys: 0.244 ± 0.106
1.466TyrAsp: 1.466 ± 0.303
1.344TyrGlu: 1.344 ± 0.247
0.55TyrPhe: 0.55 ± 0.175
2.565TyrGly: 2.565 ± 0.475
0.489TyrHis: 0.489 ± 0.137
1.038TyrIle: 1.038 ± 0.241
0.366TyrLys: 0.366 ± 0.147
1.832TyrLeu: 1.832 ± 0.243
0.305TyrMet: 0.305 ± 0.124
0.916TyrAsn: 0.916 ± 0.269
1.466TyrPro: 1.466 ± 0.301
0.794TyrGln: 0.794 ± 0.215
1.527TyrArg: 1.527 ± 0.324
1.527TyrSer: 1.527 ± 0.321
1.771TyrThr: 1.771 ± 0.303
1.405TyrVal: 1.405 ± 0.331
0.305TyrTrp: 0.305 ± 0.147
0.305TyrTyr: 0.305 ± 0.144
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (16376 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski