Cellulophaga phage phiSM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZY86|R9ZY86_9CAUD Uncharacterized protein OS=Cellulophaga phage phiSM OX=756280 GN=PhiSM_gp74 PE=4 SV=1
MM1 pKa = 7.55SNLPTPLSKK10 pKa = 9.53ITLSEE15 pKa = 4.81LIDD18 pKa = 3.66NTNEE22 pKa = 3.7NLILSIQPAGEE33 pKa = 4.02FADD36 pKa = 3.83SAKK39 pKa = 10.2LYY41 pKa = 10.94EE42 pKa = 4.19MTAVQIIEE50 pKa = 4.17HH51 pKa = 6.2LKK53 pKa = 10.03EE54 pKa = 3.81VCDD57 pKa = 3.57YY58 pKa = 9.4DD59 pKa = 3.91TYY61 pKa = 11.13IEE63 pKa = 4.08EE64 pKa = 4.58TEE66 pKa = 4.57DD67 pKa = 3.72GSLNDD72 pKa = 3.65FLDD75 pKa = 3.68MVEE78 pKa = 4.88CSDD81 pKa = 4.32GNDD84 pKa = 3.48FYY86 pKa = 11.84EE87 pKa = 4.81VIVKK91 pKa = 10.56NKK93 pKa = 5.08TTQIIFEE100 pKa = 4.76TIDD103 pKa = 3.73DD104 pKa = 4.63ADD106 pKa = 3.83TALDD110 pKa = 4.22LLEE113 pKa = 5.66DD114 pKa = 3.72SQFNEE119 pKa = 4.85DD120 pKa = 2.87IDD122 pKa = 4.09YY123 pKa = 11.24VLIYY127 pKa = 10.51AIGDD131 pKa = 4.31DD132 pKa = 3.82IPNAIEE138 pKa = 3.86FRR140 pKa = 11.84NQEE143 pKa = 3.77IYY145 pKa = 10.77QLLNNN150 pKa = 4.51

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A229|S0A229_9CAUD Uncharacterized protein OS=Cellulophaga phage phiSM OX=756280 GN=PhiSM_gp78 PE=4 SV=1
MM1 pKa = 7.26QKK3 pKa = 10.39LIKK6 pKa = 9.35PQPLKK11 pKa = 10.91FNTMKK16 pKa = 10.97KK17 pKa = 10.15LLLNAEE23 pKa = 4.25LSHH26 pKa = 7.3RR27 pKa = 11.84AACKK31 pKa = 10.18LYY33 pKa = 9.98RR34 pKa = 11.84LVLISTAASFVVALVLYY51 pKa = 10.08LLL53 pKa = 4.61

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

17537

34

1094

216.5

24.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.136 ± 0.415

0.986 ± 0.117

5.947 ± 0.241

6.974 ± 0.332

4.733 ± 0.186

5.514 ± 0.367

1.477 ± 0.12

7.316 ± 0.176

9.46 ± 0.637

8.502 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.139

6.529 ± 0.203

2.509 ± 0.149

3.176 ± 0.113

3.655 ± 0.239

7.989 ± 0.256

5.925 ± 0.242

6.495 ± 0.22

1.112 ± 0.114

3.547 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski