Amino acid dipepetide frequency for Cellulophaga phage phiSM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.246AlaAla: 5.246 ± 0.897
0.627AlaCys: 0.627 ± 0.189
3.193AlaAsp: 3.193 ± 0.369
3.65AlaGlu: 3.65 ± 0.474
2.053AlaPhe: 2.053 ± 0.429
3.821AlaGly: 3.821 ± 0.476
0.798AlaHis: 0.798 ± 0.2
5.018AlaIle: 5.018 ± 0.433
6.444AlaLys: 6.444 ± 1.216
5.703AlaLeu: 5.703 ± 0.56
0.912AlaMet: 0.912 ± 0.24
3.25AlaAsn: 3.25 ± 0.374
1.369AlaPro: 1.369 ± 0.462
1.711AlaGln: 1.711 ± 0.271
1.939AlaArg: 1.939 ± 0.319
4.733AlaSer: 4.733 ± 0.423
4.676AlaThr: 4.676 ± 0.645
4.505AlaVal: 4.505 ± 0.526
0.399AlaTrp: 0.399 ± 0.135
1.312AlaTyr: 1.312 ± 0.261
0.0AlaXaa: 0.0 ± 0.0
Cys
0.171CysAla: 0.171 ± 0.095
0.342CysCys: 0.342 ± 0.115
0.513CysAsp: 0.513 ± 0.172
0.741CysGlu: 0.741 ± 0.223
0.456CysPhe: 0.456 ± 0.174
0.684CysGly: 0.684 ± 0.219
0.0CysHis: 0.0 ± 0.0
0.798CysIle: 0.798 ± 0.253
0.855CysLys: 0.855 ± 0.213
0.855CysLeu: 0.855 ± 0.29
0.0CysMet: 0.0 ± 0.0
0.285CysAsn: 0.285 ± 0.108
0.627CysPro: 0.627 ± 0.203
0.285CysGln: 0.285 ± 0.12
0.57CysArg: 0.57 ± 0.18
0.798CysSer: 0.798 ± 0.249
0.57CysThr: 0.57 ± 0.177
0.855CysVal: 0.855 ± 0.184
0.057CysTrp: 0.057 ± 0.057
0.399CysTyr: 0.399 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
2.908AspAla: 2.908 ± 0.308
0.57AspCys: 0.57 ± 0.181
3.707AspAsp: 3.707 ± 0.55
4.163AspGlu: 4.163 ± 0.398
3.422AspPhe: 3.422 ± 0.379
3.992AspGly: 3.992 ± 0.474
0.684AspHis: 0.684 ± 0.188
4.106AspIle: 4.106 ± 0.349
4.619AspLys: 4.619 ± 0.519
5.132AspLeu: 5.132 ± 0.656
0.969AspMet: 0.969 ± 0.199
3.764AspAsn: 3.764 ± 0.467
1.312AspPro: 1.312 ± 0.223
1.026AspGln: 1.026 ± 0.194
1.996AspArg: 1.996 ± 0.342
5.646AspSer: 5.646 ± 0.569
2.965AspThr: 2.965 ± 0.43
4.277AspVal: 4.277 ± 0.505
1.255AspTrp: 1.255 ± 0.265
2.965AspTyr: 2.965 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
5.075GluAla: 5.075 ± 0.806
0.513GluCys: 0.513 ± 0.197
4.277GluAsp: 4.277 ± 0.552
5.931GluGlu: 5.931 ± 1.044
2.566GluPhe: 2.566 ± 0.301
2.965GluGly: 2.965 ± 0.383
1.255GluHis: 1.255 ± 0.258
6.444GluIle: 6.444 ± 0.561
6.615GluLys: 6.615 ± 0.752
5.76GluLeu: 5.76 ± 0.558
1.255GluMet: 1.255 ± 0.291
4.391GluAsn: 4.391 ± 0.546
1.141GluPro: 1.141 ± 0.248
2.509GluGln: 2.509 ± 0.452
1.825GluArg: 1.825 ± 0.307
5.36GluSer: 5.36 ± 0.684
4.505GluThr: 4.505 ± 0.407
5.132GluVal: 5.132 ± 0.551
0.684GluTrp: 0.684 ± 0.171
1.54GluTyr: 1.54 ± 0.247
0.0GluXaa: 0.0 ± 0.0
Phe
2.509PheAla: 2.509 ± 0.326
0.399PheCys: 0.399 ± 0.155
3.593PheAsp: 3.593 ± 0.411
2.851PheGlu: 2.851 ± 0.413
1.825PhePhe: 1.825 ± 0.258
1.939PheGly: 1.939 ± 0.267
0.855PheHis: 0.855 ± 0.225
3.707PheIle: 3.707 ± 0.417
4.847PheLys: 4.847 ± 0.581
3.707PheLeu: 3.707 ± 0.479
1.312PheMet: 1.312 ± 0.304
3.365PheAsn: 3.365 ± 0.416
1.54PhePro: 1.54 ± 0.26
1.255PheGln: 1.255 ± 0.275
1.768PheArg: 1.768 ± 0.288
4.676PheSer: 4.676 ± 0.442
2.452PheThr: 2.452 ± 0.337
2.851PheVal: 2.851 ± 0.386
0.399PheTrp: 0.399 ± 0.152
1.483PheTyr: 1.483 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
3.593GlyAla: 3.593 ± 0.587
0.513GlyCys: 0.513 ± 0.164
3.707GlyAsp: 3.707 ± 0.455
3.479GlyGlu: 3.479 ± 0.429
3.193GlyPhe: 3.193 ± 0.395
4.22GlyGly: 4.22 ± 0.762
0.627GlyHis: 0.627 ± 0.196
3.593GlyIle: 3.593 ± 0.46
4.619GlyLys: 4.619 ± 0.577
3.65GlyLeu: 3.65 ± 0.473
1.083GlyMet: 1.083 ± 0.215
3.536GlyAsn: 3.536 ± 0.493
0.228GlyPro: 0.228 ± 0.099
1.768GlyGln: 1.768 ± 0.292
2.395GlyArg: 2.395 ± 0.311
4.22GlySer: 4.22 ± 0.528
3.878GlyThr: 3.878 ± 0.479
4.106GlyVal: 4.106 ± 0.716
0.57GlyTrp: 0.57 ± 0.16
2.167GlyTyr: 2.167 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
0.741HisAla: 0.741 ± 0.206
0.228HisCys: 0.228 ± 0.105
0.513HisAsp: 0.513 ± 0.169
1.026HisGlu: 1.026 ± 0.277
0.855HisPhe: 0.855 ± 0.21
1.141HisGly: 1.141 ± 0.327
0.228HisHis: 0.228 ± 0.131
0.969HisIle: 0.969 ± 0.243
1.369HisLys: 1.369 ± 0.305
1.597HisLeu: 1.597 ± 0.28
0.399HisMet: 0.399 ± 0.128
0.855HisAsn: 0.855 ± 0.208
0.456HisPro: 0.456 ± 0.156
0.627HisGln: 0.627 ± 0.204
0.855HisArg: 0.855 ± 0.25
0.855HisSer: 0.855 ± 0.197
0.456HisThr: 0.456 ± 0.139
0.627HisVal: 0.627 ± 0.197
0.342HisTrp: 0.342 ± 0.117
0.627HisTyr: 0.627 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
3.992IleAla: 3.992 ± 0.49
0.57IleCys: 0.57 ± 0.175
5.646IleAsp: 5.646 ± 0.494
6.159IleGlu: 6.159 ± 0.702
2.452IlePhe: 2.452 ± 0.356
3.193IleGly: 3.193 ± 0.458
1.026IleHis: 1.026 ± 0.242
4.22IleIle: 4.22 ± 0.458
7.071IleLys: 7.071 ± 0.551
4.961IleLeu: 4.961 ± 0.563
1.54IleMet: 1.54 ± 0.249
5.531IleAsn: 5.531 ± 0.453
2.452IlePro: 2.452 ± 0.263
1.939IleGln: 1.939 ± 0.261
2.623IleArg: 2.623 ± 0.435
5.988IleSer: 5.988 ± 0.507
5.132IleThr: 5.132 ± 0.612
4.79IleVal: 4.79 ± 0.446
0.798IleTrp: 0.798 ± 0.168
3.079IleTyr: 3.079 ± 0.524
0.0IleXaa: 0.0 ± 0.0
Lys
6.216LysAla: 6.216 ± 0.679
0.855LysCys: 0.855 ± 0.191
4.733LysAsp: 4.733 ± 0.712
7.014LysGlu: 7.014 ± 1.053
4.505LysPhe: 4.505 ± 0.517
5.075LysGly: 5.075 ± 0.654
2.053LysHis: 2.053 ± 0.353
6.216LysIle: 6.216 ± 0.583
11.861LysLys: 11.861 ± 1.984
7.527LysLeu: 7.527 ± 0.785
2.794LysMet: 2.794 ± 0.469
5.76LysAsn: 5.76 ± 0.493
1.483LysPro: 1.483 ± 0.211
2.965LysGln: 2.965 ± 0.404
3.136LysArg: 3.136 ± 0.495
6.558LysSer: 6.558 ± 0.574
6.273LysThr: 6.273 ± 0.646
5.36LysVal: 5.36 ± 0.568
1.083LysTrp: 1.083 ± 0.244
3.136LysTyr: 3.136 ± 0.428
0.0LysXaa: 0.0 ± 0.0
Leu
4.733LeuAla: 4.733 ± 0.529
1.312LeuCys: 1.312 ± 0.222
5.531LeuAsp: 5.531 ± 0.597
5.817LeuGlu: 5.817 ± 0.59
3.136LeuPhe: 3.136 ± 0.389
4.505LeuGly: 4.505 ± 0.516
1.083LeuHis: 1.083 ± 0.265
6.501LeuIle: 6.501 ± 0.726
8.839LeuLys: 8.839 ± 0.742
5.874LeuLeu: 5.874 ± 0.556
2.281LeuMet: 2.281 ± 0.368
5.988LeuAsn: 5.988 ± 0.651
2.68LeuPro: 2.68 ± 0.358
2.452LeuGln: 2.452 ± 0.355
3.365LeuArg: 3.365 ± 0.377
7.698LeuSer: 7.698 ± 0.813
4.049LeuThr: 4.049 ± 0.426
5.132LeuVal: 5.132 ± 0.411
1.083LeuTrp: 1.083 ± 0.245
2.908LeuTyr: 2.908 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
1.369MetAla: 1.369 ± 0.275
0.114MetCys: 0.114 ± 0.088
0.684MetAsp: 0.684 ± 0.182
1.483MetGlu: 1.483 ± 0.285
0.741MetPhe: 0.741 ± 0.179
0.741MetGly: 0.741 ± 0.244
0.456MetHis: 0.456 ± 0.15
0.855MetIle: 0.855 ± 0.175
2.395MetLys: 2.395 ± 0.304
1.083MetLeu: 1.083 ± 0.249
0.513MetMet: 0.513 ± 0.16
1.825MetAsn: 1.825 ± 0.303
0.627MetPro: 0.627 ± 0.214
1.426MetGln: 1.426 ± 0.237
1.198MetArg: 1.198 ± 0.25
1.996MetSer: 1.996 ± 0.29
1.255MetThr: 1.255 ± 0.28
0.855MetVal: 0.855 ± 0.218
0.057MetTrp: 0.057 ± 0.059
0.513MetTyr: 0.513 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.479AsnAla: 3.479 ± 0.58
0.684AsnCys: 0.684 ± 0.221
3.365AsnAsp: 3.365 ± 0.403
4.961AsnGlu: 4.961 ± 0.521
3.25AsnPhe: 3.25 ± 0.474
3.764AsnGly: 3.764 ± 0.484
0.456AsnHis: 0.456 ± 0.139
4.334AsnIle: 4.334 ± 0.449
6.33AsnLys: 6.33 ± 0.502
6.387AsnLeu: 6.387 ± 0.501
1.312AsnMet: 1.312 ± 0.286
4.163AsnAsn: 4.163 ± 0.415
1.939AsnPro: 1.939 ± 0.317
2.623AsnGln: 2.623 ± 0.384
2.338AsnArg: 2.338 ± 0.348
5.018AsnSer: 5.018 ± 0.597
3.25AsnThr: 3.25 ± 0.463
4.22AsnVal: 4.22 ± 0.527
0.741AsnTrp: 0.741 ± 0.179
2.68AsnTyr: 2.68 ± 0.322
0.0AsnXaa: 0.0 ± 0.0
Pro
1.597ProAla: 1.597 ± 0.326
0.285ProCys: 0.285 ± 0.123
1.939ProAsp: 1.939 ± 0.31
2.11ProGlu: 2.11 ± 0.346
1.483ProPhe: 1.483 ± 0.289
0.741ProGly: 0.741 ± 0.19
0.399ProHis: 0.399 ± 0.187
1.312ProIle: 1.312 ± 0.291
1.654ProLys: 1.654 ± 0.292
2.452ProLeu: 2.452 ± 0.351
0.456ProMet: 0.456 ± 0.174
1.54ProAsn: 1.54 ± 0.305
0.627ProPro: 0.627 ± 0.207
0.912ProGln: 0.912 ± 0.235
0.399ProArg: 0.399 ± 0.118
2.167ProSer: 2.167 ± 0.397
1.825ProThr: 1.825 ± 0.358
2.11ProVal: 2.11 ± 0.418
0.171ProTrp: 0.171 ± 0.102
0.912ProTyr: 0.912 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
2.452GlnAla: 2.452 ± 0.357
0.228GlnCys: 0.228 ± 0.118
1.654GlnAsp: 1.654 ± 0.246
1.996GlnGlu: 1.996 ± 0.331
1.654GlnPhe: 1.654 ± 0.248
1.711GlnGly: 1.711 ± 0.293
0.627GlnHis: 0.627 ± 0.283
2.566GlnIle: 2.566 ± 0.359
2.281GlnLys: 2.281 ± 0.324
3.479GlnLeu: 3.479 ± 0.486
0.627GlnMet: 0.627 ± 0.198
2.053GlnAsn: 2.053 ± 0.341
0.57GlnPro: 0.57 ± 0.166
1.255GlnGln: 1.255 ± 0.21
1.312GlnArg: 1.312 ± 0.28
2.11GlnSer: 2.11 ± 0.274
1.711GlnThr: 1.711 ± 0.26
1.882GlnVal: 1.882 ± 0.366
0.285GlnTrp: 0.285 ± 0.126
1.312GlnTyr: 1.312 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
2.509ArgAla: 2.509 ± 0.385
0.285ArgCys: 0.285 ± 0.119
2.11ArgAsp: 2.11 ± 0.273
1.312ArgGlu: 1.312 ± 0.271
2.11ArgPhe: 2.11 ± 0.322
1.597ArgGly: 1.597 ± 0.247
0.456ArgHis: 0.456 ± 0.142
3.307ArgIle: 3.307 ± 0.41
3.65ArgLys: 3.65 ± 0.503
3.307ArgLeu: 3.307 ± 0.358
0.798ArgMet: 0.798 ± 0.229
2.68ArgAsn: 2.68 ± 0.379
0.741ArgPro: 0.741 ± 0.164
1.369ArgGln: 1.369 ± 0.262
1.939ArgArg: 1.939 ± 0.308
2.224ArgSer: 2.224 ± 0.381
2.11ArgThr: 2.11 ± 0.317
1.996ArgVal: 1.996 ± 0.316
0.627ArgTrp: 0.627 ± 0.227
1.426ArgTyr: 1.426 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
4.562SerAla: 4.562 ± 0.597
0.57SerCys: 0.57 ± 0.246
4.562SerAsp: 4.562 ± 0.513
4.961SerGlu: 4.961 ± 0.488
5.246SerPhe: 5.246 ± 0.563
5.531SerGly: 5.531 ± 0.594
1.426SerHis: 1.426 ± 0.318
5.189SerIle: 5.189 ± 0.501
6.672SerLys: 6.672 ± 0.817
6.843SerLeu: 6.843 ± 0.665
1.312SerMet: 1.312 ± 0.23
5.36SerAsn: 5.36 ± 0.588
1.825SerPro: 1.825 ± 0.317
2.452SerGln: 2.452 ± 0.269
2.908SerArg: 2.908 ± 0.36
7.014SerSer: 7.014 ± 0.692
4.961SerThr: 4.961 ± 0.529
5.075SerVal: 5.075 ± 0.482
0.798SerTrp: 0.798 ± 0.205
2.623SerTyr: 2.623 ± 0.4
0.0SerXaa: 0.0 ± 0.0
Thr
3.365ThrAla: 3.365 ± 0.524
0.513ThrCys: 0.513 ± 0.224
3.307ThrAsp: 3.307 ± 0.514
3.935ThrGlu: 3.935 ± 0.443
2.794ThrPhe: 2.794 ± 0.441
3.821ThrGly: 3.821 ± 0.518
0.912ThrHis: 0.912 ± 0.207
5.703ThrIle: 5.703 ± 0.475
4.79ThrLys: 4.79 ± 0.497
6.216ThrLeu: 6.216 ± 0.561
0.57ThrMet: 0.57 ± 0.165
2.965ThrAsn: 2.965 ± 0.427
2.452ThrPro: 2.452 ± 0.338
1.768ThrGln: 1.768 ± 0.305
1.996ThrArg: 1.996 ± 0.334
4.391ThrSer: 4.391 ± 0.4
3.136ThrThr: 3.136 ± 0.369
3.707ThrVal: 3.707 ± 0.409
0.57ThrTrp: 0.57 ± 0.157
2.338ThrTyr: 2.338 ± 0.406
0.0ThrXaa: 0.0 ± 0.0
Val
4.619ValAla: 4.619 ± 0.92
0.627ValCys: 0.627 ± 0.194
3.992ValAsp: 3.992 ± 0.501
5.075ValGlu: 5.075 ± 0.684
3.193ValPhe: 3.193 ± 0.39
3.136ValGly: 3.136 ± 0.477
0.855ValHis: 0.855 ± 0.246
5.018ValIle: 5.018 ± 0.483
5.931ValLys: 5.931 ± 0.455
5.589ValLeu: 5.589 ± 0.601
1.083ValMet: 1.083 ± 0.245
4.847ValAsn: 4.847 ± 0.485
1.483ValPro: 1.483 ± 0.238
1.654ValGln: 1.654 ± 0.279
2.053ValArg: 2.053 ± 0.283
5.132ValSer: 5.132 ± 0.572
3.707ValThr: 3.707 ± 0.483
4.22ValVal: 4.22 ± 0.532
0.57ValTrp: 0.57 ± 0.162
2.167ValTyr: 2.167 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
0.798TrpAla: 0.798 ± 0.164
0.171TrpCys: 0.171 ± 0.091
0.684TrpAsp: 0.684 ± 0.181
0.855TrpGlu: 0.855 ± 0.199
0.684TrpPhe: 0.684 ± 0.201
0.57TrpGly: 0.57 ± 0.213
0.171TrpHis: 0.171 ± 0.091
0.513TrpIle: 0.513 ± 0.163
0.513TrpLys: 0.513 ± 0.148
1.141TrpLeu: 1.141 ± 0.223
0.171TrpMet: 0.171 ± 0.089
0.627TrpAsn: 0.627 ± 0.226
0.171TrpPro: 0.171 ± 0.101
0.456TrpGln: 0.456 ± 0.15
0.513TrpArg: 0.513 ± 0.155
0.969TrpSer: 0.969 ± 0.266
0.627TrpThr: 0.627 ± 0.194
1.026TrpVal: 1.026 ± 0.214
0.171TrpTrp: 0.171 ± 0.103
0.285TrpTyr: 0.285 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.426TyrAla: 1.426 ± 0.264
0.456TyrCys: 0.456 ± 0.199
1.768TyrAsp: 1.768 ± 0.27
2.224TyrGlu: 2.224 ± 0.342
1.711TyrPhe: 1.711 ± 0.331
1.996TyrGly: 1.996 ± 0.357
0.399TyrHis: 0.399 ± 0.16
2.794TyrIle: 2.794 ± 0.425
3.25TyrLys: 3.25 ± 0.397
3.764TyrLeu: 3.764 ± 0.436
0.741TyrMet: 0.741 ± 0.213
2.509TyrAsn: 2.509 ± 0.341
1.369TyrPro: 1.369 ± 0.299
1.312TyrGln: 1.312 ± 0.299
1.426TyrArg: 1.426 ± 0.252
2.338TyrSer: 2.338 ± 0.325
1.711TyrThr: 1.711 ± 0.306
2.224TyrVal: 2.224 ± 0.346
0.456TyrTrp: 0.456 ± 0.162
1.597TyrTyr: 1.597 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (17537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski