Chryseobacterium piperi
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3502 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A086BAY3|A0A086BAY3_9FLAO Hydroxyacid dehydrogenase OS=Chryseobacterium piperi OX=558152 GN=IQ37_11675 PE=3 SV=1
MM1 pKa = 7.78 KK2 pKa = 9.23 NTLLLKK8 pKa = 9.68 TIILTLVLSSYY19 pKa = 11.88 SMVKK23 pKa = 10.11 AQYY26 pKa = 10.66 DD27 pKa = 3.84 PNQDD31 pKa = 3.28 LDD33 pKa = 3.72 GDD35 pKa = 4.6 GIINSIDD42 pKa = 4.16 LDD44 pKa = 4.37 DD45 pKa = 6.14 DD46 pKa = 4.09 NDD48 pKa = 5.11 GIPDD52 pKa = 4.01 LLEE55 pKa = 4.5 SPPSNTVVNGTFTGTSAPWVLGTGWVFNSGGGNVRR90 pKa = 11.84 IEE92 pKa = 4.1 TDD94 pKa = 3.17 NVSNRR99 pKa = 11.84 DD100 pKa = 3.17 LRR102 pKa = 11.84 QTVNNLNRR110 pKa = 11.84 TNGTIALTMRR120 pKa = 11.84 LGAQDD125 pKa = 3.72 GSNAAGSTASLQVILNGTVYY145 pKa = 10.0 ATINNGTTRR154 pKa = 11.84 STATNNITITLANGATSNFTPYY176 pKa = 7.72 TTAVATGYY184 pKa = 8.34 TFQTFTINIPYY195 pKa = 9.63 NSPGTADD202 pKa = 4.73 LIFRR206 pKa = 11.84 ATTVLDD212 pKa = 3.91 DD213 pKa = 3.48 WTLDD217 pKa = 4.26 DD218 pKa = 3.69 IAIPAFIEE226 pKa = 4.31 DD227 pKa = 4.05 TDD229 pKa = 4.32 GDD231 pKa = 4.69 GIPNYY236 pKa = 10.14 QDD238 pKa = 4.45 LDD240 pKa = 3.95 SDD242 pKa = 4.51 NDD244 pKa = 3.81 GCLDD248 pKa = 4.14 AMEE251 pKa = 5.0 GDD253 pKa = 4.02 EE254 pKa = 4.06 NVAYY258 pKa = 10.97 SMLVAAAPGLTVGTGSTAPNQNLCSTGACVDD289 pKa = 3.57 AQGVPVVVNAGGAADD304 pKa = 3.7 IGSDD308 pKa = 3.23 QGQGIGDD315 pKa = 3.9 SKK317 pKa = 11.49 NNAVIACYY325 pKa = 9.89 CYY327 pKa = 10.66 KK328 pKa = 10.49 PVVTAGTALNTPYY341 pKa = 10.94 GITALGRR348 pKa = 11.84 AGANSGNWPMVRR360 pKa = 11.84 KK361 pKa = 9.28 GAWTALEE368 pKa = 4.33 AKK370 pKa = 9.6 TKK372 pKa = 10.55 GFVPNRR378 pKa = 11.84 LTSTQISAIPAANLVEE394 pKa = 4.66 GMMVYY399 pKa = 8.62 NTTLDD404 pKa = 3.75 CLQVNTNGTASGWACFNTQTCPTNN428 pKa = 3.32
Molecular weight: 44.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.656
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.884
Rodwell 3.719
Grimsley 3.554
Solomon 3.897
Lehninger 3.859
Nozaki 4.012
DTASelect 4.329
Thurlkill 3.719
EMBOSS 3.884
Sillero 4.024
Patrickios 1.252
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A086BLU0|A0A086BLU0_9FLAO Leucine--tRNA ligase OS=Chryseobacterium piperi OX=558152 GN=leuS PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.59 RR11 pKa = 11.84 KK12 pKa = 9.33 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 10.03 HH17 pKa = 3.97 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.01 GRR40 pKa = 11.84 KK41 pKa = 9.03 SLTISAARR49 pKa = 11.84 AKK51 pKa = 10.4 RR52 pKa = 3.38
Molecular weight: 6.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 10.774
IPC_protein 12.369
Toseland 12.53
ProMoST 13.027
Dawson 12.53
Bjellqvist 12.53
Wikipedia 13.013
Rodwell 12.223
Grimsley 12.574
Solomon 13.027
Lehninger 12.925
Nozaki 12.53
DTASelect 12.53
Thurlkill 12.53
EMBOSS 13.027
Sillero 12.53
Patrickios 11.959
IPC_peptide 13.027
IPC2_peptide 12.018
IPC2.peptide.svr19 9.11
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3502
0
3502
1174652
51
2623
335.4
37.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.152 ± 0.039
0.695 ± 0.012
5.358 ± 0.029
6.498 ± 0.054
5.315 ± 0.034
6.489 ± 0.039
1.724 ± 0.018
7.981 ± 0.04
8.086 ± 0.046
9.056 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.356 ± 0.019
6.315 ± 0.047
3.508 ± 0.022
3.619 ± 0.025
3.365 ± 0.027
6.601 ± 0.032
5.563 ± 0.036
6.002 ± 0.029
1.082 ± 0.015
4.236 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here