Trichuris suis (pig whipworm)
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3288 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B1PI24|A0A0B1PI24_9BILA Metalloendopeptidase OS=Trichuris suis OX=68888 GN=D918_08099 PE=4 SV=1
MM1 pKa = 7.79 YY2 pKa = 10.42 EE3 pKa = 4.32 EE4 pKa = 4.32 YY5 pKa = 11.15 NKK7 pKa = 10.13 FYY9 pKa = 11.02 EE10 pKa = 4.25 LFEE13 pKa = 4.49 PVLSFFADD21 pKa = 3.28 EE22 pKa = 5.06 DD23 pKa = 3.89 VTLRR27 pKa = 11.84 DD28 pKa = 3.28 ILEE31 pKa = 4.13 LRR33 pKa = 11.84 EE34 pKa = 3.68 ADD36 pKa = 3.03 IAYY39 pKa = 8.33 WADD42 pKa = 3.24 LCEE45 pKa = 4.61 KK46 pKa = 9.95 FNVMNLQLQVGNLNN60 pKa = 3.5
Molecular weight: 7.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 3.986
IPC_protein 3.834
Toseland 3.668
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.592
Solomon 3.783
Lehninger 3.745
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A085N6M9|A0A085N6M9_9BILA Uncharacterized protein OS=Trichuris suis OX=68888 GN=D918_07103 PE=4 SV=1
MM1 pKa = 6.86 NAAWMSVRR9 pKa = 11.84 SIVVIQTRR17 pKa = 11.84 IRR19 pKa = 11.84 GGTGRR24 pKa = 11.84 GKK26 pKa = 10.62 APGRR30 pKa = 11.84 GRR32 pKa = 11.84 GPGRR36 pKa = 11.84 GRR38 pKa = 11.84 GRR40 pKa = 11.84 GPGRR44 pKa = 11.84 GRR46 pKa = 11.84 GSGPGRR52 pKa = 11.84 GGGSGPGRR60 pKa = 11.84 GRR62 pKa = 11.84 GSGPGRR68 pKa = 11.84 GTGSGGGSGFRR79 pKa = 11.84 MGPRR83 pKa = 11.84 RR84 pKa = 11.84 LPGPVAWGVCLFF96 pKa = 4.25
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.427
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.149
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3288
0
3288
1291231
26
6770
392.7
44.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.045 ± 0.034
2.696 ± 0.044
5.216 ± 0.028
6.31 ± 0.042
4.295 ± 0.03
5.571 ± 0.055
2.361 ± 0.018
4.876 ± 0.029
5.603 ± 0.038
9.66 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.016
4.219 ± 0.023
4.822 ± 0.045
4.023 ± 0.027
6.263 ± 0.038
8.303 ± 0.055
5.229 ± 0.029
6.784 ± 0.035
1.253 ± 0.015
3.064 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here