Gordonia phage Squiddly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Langleyhallvirinae; unclassified Langleyhallvirinae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U1I1|A0A514U1I1_9CAUD Antirepressor OS=Gordonia phage Squiddly OX=2593337 GN=49 PE=4 SV=1
MM1 pKa = 7.33ATLKK5 pKa = 10.26RR6 pKa = 11.84WNGTAWRR13 pKa = 11.84PVGEE17 pKa = 4.28DD18 pKa = 3.06TYY20 pKa = 11.78APLMPDD26 pKa = 2.97TGITYY31 pKa = 10.26DD32 pKa = 3.47GSGNVQTVTEE42 pKa = 4.23NGVMTTYY49 pKa = 10.21TYY51 pKa = 11.46NPDD54 pKa = 2.94GTVATDD60 pKa = 3.14TRR62 pKa = 11.84GGVTRR67 pKa = 11.84TYY69 pKa = 10.98GYY71 pKa = 10.7DD72 pKa = 3.3GNGNLTSITVEE83 pKa = 4.16DD84 pKa = 3.72

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U1J8|A0A514U1J8_9CAUD Uncharacterized protein OS=Gordonia phage Squiddly OX=2593337 GN=55 PE=4 SV=1
MM1 pKa = 6.91STSEE5 pKa = 3.6QVAARR10 pKa = 11.84RR11 pKa = 11.84QKK13 pKa = 9.96VAEE16 pKa = 4.32LTRR19 pKa = 11.84QGCSTPHH26 pKa = 6.81IARR29 pKa = 11.84ICGATPEE36 pKa = 4.41TIRR39 pKa = 11.84RR40 pKa = 11.84DD41 pKa = 3.39RR42 pKa = 11.84RR43 pKa = 11.84ALGISQGRR51 pKa = 11.84APDD54 pKa = 3.92LTPQQLDD61 pKa = 3.51TARR64 pKa = 11.84QLLADD69 pKa = 4.05GCSYY73 pKa = 11.3RR74 pKa = 11.84EE75 pKa = 3.72TARR78 pKa = 11.84TIGCGHH84 pKa = 6.19SALRR88 pKa = 11.84RR89 pKa = 11.84HH90 pKa = 6.18FPGQGWTLSQSASWGMYY107 pKa = 8.07IRR109 pKa = 11.84KK110 pKa = 8.88HH111 pKa = 5.87ASS113 pKa = 2.71

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

18406

18

1870

175.3

19.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.127 ± 0.417

1.358 ± 0.172

6.748 ± 0.31

5.813 ± 0.314

2.64 ± 0.188

8.313 ± 0.362

2.233 ± 0.17

4.52 ± 0.155

3.635 ± 0.225

7.617 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.104

2.95 ± 0.152

5.629 ± 0.213

3.83 ± 0.163

7.101 ± 0.313

5.667 ± 0.204

6.683 ± 0.291

6.916 ± 0.246

2.304 ± 0.116

2.412 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski