Amino acid dipepetide frequency for Gordonia phage Squiddly

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.964AlaAla: 13.964 ± 1.196
1.032AlaCys: 1.032 ± 0.332
7.824AlaAsp: 7.824 ± 0.782
7.498AlaGlu: 7.498 ± 0.63
3.151AlaPhe: 3.151 ± 0.474
9.508AlaGly: 9.508 ± 0.857
2.01AlaHis: 2.01 ± 0.371
5.596AlaIle: 5.596 ± 0.554
3.477AlaLys: 3.477 ± 0.547
8.802AlaLeu: 8.802 ± 0.692
3.477AlaMet: 3.477 ± 0.426
3.423AlaAsn: 3.423 ± 0.416
5.814AlaPro: 5.814 ± 0.604
3.966AlaGln: 3.966 ± 0.54
7.607AlaArg: 7.607 ± 0.708
5.433AlaSer: 5.433 ± 0.47
6.846AlaThr: 6.846 ± 0.653
7.824AlaVal: 7.824 ± 1.068
1.847AlaTrp: 1.847 ± 0.327
2.173AlaTyr: 2.173 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
1.304CysAla: 1.304 ± 0.335
0.217CysCys: 0.217 ± 0.107
0.815CysAsp: 0.815 ± 0.283
1.195CysGlu: 1.195 ± 0.319
0.109CysPhe: 0.109 ± 0.094
1.793CysGly: 1.793 ± 0.395
0.543CysHis: 0.543 ± 0.176
0.272CysIle: 0.272 ± 0.126
0.489CysLys: 0.489 ± 0.174
0.761CysLeu: 0.761 ± 0.196
0.054CysMet: 0.054 ± 0.056
0.489CysAsn: 0.489 ± 0.198
0.924CysPro: 0.924 ± 0.274
0.706CysGln: 0.706 ± 0.255
0.978CysArg: 0.978 ± 0.253
0.815CysSer: 0.815 ± 0.308
0.652CysThr: 0.652 ± 0.198
0.815CysVal: 0.815 ± 0.226
0.435CysTrp: 0.435 ± 0.166
0.217CysTyr: 0.217 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
7.661AspAla: 7.661 ± 0.553
0.543AspCys: 0.543 ± 0.189
3.858AspAsp: 3.858 ± 0.561
4.727AspGlu: 4.727 ± 0.491
1.413AspPhe: 1.413 ± 0.265
6.357AspGly: 6.357 ± 0.706
1.63AspHis: 1.63 ± 0.287
2.934AspIle: 2.934 ± 0.44
1.413AspLys: 1.413 ± 0.293
6.52AspLeu: 6.52 ± 0.655
1.576AspMet: 1.576 ± 0.307
1.902AspAsn: 1.902 ± 0.298
3.912AspPro: 3.912 ± 0.429
1.684AspGln: 1.684 ± 0.326
5.162AspArg: 5.162 ± 0.614
3.803AspSer: 3.803 ± 0.41
4.184AspThr: 4.184 ± 0.481
5.162AspVal: 5.162 ± 0.551
1.25AspTrp: 1.25 ± 0.246
1.793AspTyr: 1.793 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
6.194GluAla: 6.194 ± 0.622
0.761GluCys: 0.761 ± 0.234
3.151GluAsp: 3.151 ± 0.448
2.499GluGlu: 2.499 ± 0.402
1.684GluPhe: 1.684 ± 0.323
3.695GluGly: 3.695 ± 0.515
1.358GluHis: 1.358 ± 0.222
2.771GluIle: 2.771 ± 0.354
2.445GluLys: 2.445 ± 0.41
5.596GluLeu: 5.596 ± 0.782
1.087GluMet: 1.087 ± 0.239
1.25GluAsn: 1.25 ± 0.201
3.64GluPro: 3.64 ± 0.599
3.097GluGln: 3.097 ± 0.403
4.781GluArg: 4.781 ± 0.569
2.988GluSer: 2.988 ± 0.401
3.477GluThr: 3.477 ± 0.415
4.51GluVal: 4.51 ± 0.516
1.63GluTrp: 1.63 ± 0.369
1.521GluTyr: 1.521 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
2.88PheAla: 2.88 ± 0.361
0.489PheCys: 0.489 ± 0.158
2.119PheAsp: 2.119 ± 0.344
1.304PheGlu: 1.304 ± 0.241
0.706PhePhe: 0.706 ± 0.184
2.336PheGly: 2.336 ± 0.338
0.761PheHis: 0.761 ± 0.179
0.869PheIle: 0.869 ± 0.223
0.978PheLys: 0.978 ± 0.221
1.63PheLeu: 1.63 ± 0.355
0.815PheMet: 0.815 ± 0.26
1.087PheAsn: 1.087 ± 0.259
1.032PhePro: 1.032 ± 0.288
1.141PheGln: 1.141 ± 0.241
2.065PheArg: 2.065 ± 0.383
1.141PheSer: 1.141 ± 0.255
2.065PheThr: 2.065 ± 0.279
1.63PheVal: 1.63 ± 0.296
0.435PheTrp: 0.435 ± 0.154
0.924PheTyr: 0.924 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
7.444GlyAla: 7.444 ± 1.117
1.032GlyCys: 1.032 ± 0.275
5.488GlyAsp: 5.488 ± 0.572
4.836GlyGlu: 4.836 ± 0.543
3.206GlyPhe: 3.206 ± 0.513
7.824GlyGly: 7.824 ± 0.935
1.793GlyHis: 1.793 ± 0.338
4.727GlyIle: 4.727 ± 0.554
3.314GlyLys: 3.314 ± 0.452
5.759GlyLeu: 5.759 ± 0.566
2.554GlyMet: 2.554 ± 0.325
2.934GlyAsn: 2.934 ± 0.437
3.206GlyPro: 3.206 ± 0.403
2.825GlyGln: 2.825 ± 0.302
5.488GlyArg: 5.488 ± 0.5
4.89GlySer: 4.89 ± 0.535
4.51GlyThr: 4.51 ± 0.517
6.194GlyVal: 6.194 ± 0.741
2.771GlyTrp: 2.771 ± 0.321
2.336GlyTyr: 2.336 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.956HisAla: 1.956 ± 0.317
0.489HisCys: 0.489 ± 0.145
1.576HisAsp: 1.576 ± 0.335
1.25HisGlu: 1.25 ± 0.3
0.652HisPhe: 0.652 ± 0.158
1.793HisGly: 1.793 ± 0.299
0.761HisHis: 0.761 ± 0.231
1.087HisIle: 1.087 ± 0.23
0.435HisLys: 0.435 ± 0.148
1.739HisLeu: 1.739 ± 0.304
0.598HisMet: 0.598 ± 0.157
0.326HisAsn: 0.326 ± 0.115
1.195HisPro: 1.195 ± 0.221
1.032HisGln: 1.032 ± 0.235
1.521HisArg: 1.521 ± 0.326
1.25HisSer: 1.25 ± 0.224
1.63HisThr: 1.63 ± 0.368
1.684HisVal: 1.684 ± 0.283
0.326HisTrp: 0.326 ± 0.143
1.032HisTyr: 1.032 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
5.488IleAla: 5.488 ± 0.657
0.598IleCys: 0.598 ± 0.208
3.151IleAsp: 3.151 ± 0.415
3.097IleGlu: 3.097 ± 0.511
1.032IlePhe: 1.032 ± 0.278
3.532IleGly: 3.532 ± 0.509
1.032IleHis: 1.032 ± 0.213
1.576IleIle: 1.576 ± 0.315
1.684IleLys: 1.684 ± 0.609
2.988IleLeu: 2.988 ± 0.401
0.38IleMet: 0.38 ± 0.138
1.63IleAsn: 1.63 ± 0.271
2.662IlePro: 2.662 ± 0.334
1.467IleGln: 1.467 ± 0.197
3.966IleArg: 3.966 ± 0.378
2.499IleSer: 2.499 ± 0.377
3.26IleThr: 3.26 ± 0.39
3.369IleVal: 3.369 ± 0.435
0.815IleTrp: 0.815 ± 0.198
0.978IleTyr: 0.978 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
4.292LysAla: 4.292 ± 0.654
0.38LysCys: 0.38 ± 0.157
2.499LysAsp: 2.499 ± 0.427
1.684LysGlu: 1.684 ± 0.289
0.761LysPhe: 0.761 ± 0.201
2.662LysGly: 2.662 ± 0.46
0.978LysHis: 0.978 ± 0.209
1.413LysIle: 1.413 ± 0.289
2.119LysLys: 2.119 ± 0.408
2.825LysLeu: 2.825 ± 0.418
0.815LysMet: 0.815 ± 0.264
1.032LysAsn: 1.032 ± 0.222
2.173LysPro: 2.173 ± 0.328
1.141LysGln: 1.141 ± 0.252
3.206LysArg: 3.206 ± 0.524
2.173LysSer: 2.173 ± 0.299
2.445LysThr: 2.445 ± 0.469
2.065LysVal: 2.065 ± 0.284
0.869LysTrp: 0.869 ± 0.247
0.815LysTyr: 0.815 ± 0.21
0.0LysXaa: 0.0 ± 0.0
Leu
11.03LeuAla: 11.03 ± 0.891
1.195LeuCys: 1.195 ± 0.328
4.89LeuAsp: 4.89 ± 0.627
4.129LeuGlu: 4.129 ± 0.633
1.847LeuPhe: 1.847 ± 0.321
6.792LeuGly: 6.792 ± 0.639
1.576LeuHis: 1.576 ± 0.286
3.477LeuIle: 3.477 ± 0.4
2.825LeuLys: 2.825 ± 0.33
4.673LeuLeu: 4.673 ± 0.684
1.413LeuMet: 1.413 ± 0.227
1.956LeuAsn: 1.956 ± 0.282
4.727LeuPro: 4.727 ± 0.54
2.499LeuGln: 2.499 ± 0.394
4.836LeuArg: 4.836 ± 0.675
4.129LeuSer: 4.129 ± 0.399
5.325LeuThr: 5.325 ± 0.595
5.868LeuVal: 5.868 ± 0.614
1.793LeuTrp: 1.793 ± 0.301
1.195LeuTyr: 1.195 ± 0.223
0.0LeuXaa: 0.0 ± 0.0
Met
2.988MetAla: 2.988 ± 0.479
0.272MetCys: 0.272 ± 0.164
1.087MetAsp: 1.087 ± 0.19
0.761MetGlu: 0.761 ± 0.212
0.326MetPhe: 0.326 ± 0.135
1.847MetGly: 1.847 ± 0.331
0.435MetHis: 0.435 ± 0.169
0.815MetIle: 0.815 ± 0.197
1.032MetLys: 1.032 ± 0.22
1.63MetLeu: 1.63 ± 0.311
0.761MetMet: 0.761 ± 0.191
0.761MetAsn: 0.761 ± 0.185
1.521MetPro: 1.521 ± 0.301
1.141MetGln: 1.141 ± 0.241
1.847MetArg: 1.847 ± 0.332
2.554MetSer: 2.554 ± 0.413
3.369MetThr: 3.369 ± 0.488
1.195MetVal: 1.195 ± 0.24
0.326MetTrp: 0.326 ± 0.146
0.38MetTyr: 0.38 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
3.477AsnAla: 3.477 ± 0.387
0.543AsnCys: 0.543 ± 0.161
1.63AsnAsp: 1.63 ± 0.357
1.141AsnGlu: 1.141 ± 0.274
0.598AsnPhe: 0.598 ± 0.173
3.314AsnGly: 3.314 ± 0.445
0.761AsnHis: 0.761 ± 0.193
1.141AsnIle: 1.141 ± 0.284
0.435AsnLys: 0.435 ± 0.141
2.445AsnLeu: 2.445 ± 0.354
0.326AsnMet: 0.326 ± 0.127
0.761AsnAsn: 0.761 ± 0.251
2.499AsnPro: 2.499 ± 0.3
1.195AsnGln: 1.195 ± 0.222
2.228AsnArg: 2.228 ± 0.363
1.521AsnSer: 1.521 ± 0.248
2.391AsnThr: 2.391 ± 0.366
1.793AsnVal: 1.793 ± 0.357
0.598AsnTrp: 0.598 ± 0.173
0.706AsnTyr: 0.706 ± 0.238
0.0AsnXaa: 0.0 ± 0.0
Pro
5.596ProAla: 5.596 ± 0.513
1.25ProCys: 1.25 ± 0.333
4.944ProAsp: 4.944 ± 0.576
4.401ProGlu: 4.401 ± 0.517
1.413ProPhe: 1.413 ± 0.326
4.401ProGly: 4.401 ± 0.483
1.358ProHis: 1.358 ± 0.273
2.173ProIle: 2.173 ± 0.406
2.554ProLys: 2.554 ± 0.41
2.717ProLeu: 2.717 ± 0.329
1.467ProMet: 1.467 ± 0.245
2.01ProAsn: 2.01 ± 0.352
3.043ProPro: 3.043 ± 0.567
2.173ProGln: 2.173 ± 0.477
3.043ProArg: 3.043 ± 0.497
3.64ProSer: 3.64 ± 0.42
4.021ProThr: 4.021 ± 0.508
3.477ProVal: 3.477 ± 0.352
1.141ProTrp: 1.141 ± 0.239
1.467ProTyr: 1.467 ± 0.32
0.0ProXaa: 0.0 ± 0.0
Gln
3.858GlnAla: 3.858 ± 0.613
0.652GlnCys: 0.652 ± 0.218
1.576GlnAsp: 1.576 ± 0.284
1.521GlnGlu: 1.521 ± 0.255
1.521GlnPhe: 1.521 ± 0.272
2.445GlnGly: 2.445 ± 0.327
0.924GlnHis: 0.924 ± 0.238
2.336GlnIle: 2.336 ± 0.379
1.141GlnLys: 1.141 ± 0.279
4.673GlnLeu: 4.673 ± 0.465
1.793GlnMet: 1.793 ± 0.29
0.869GlnAsn: 0.869 ± 0.242
1.847GlnPro: 1.847 ± 0.298
2.01GlnGln: 2.01 ± 0.262
2.934GlnArg: 2.934 ± 0.354
2.119GlnSer: 2.119 ± 0.36
1.902GlnThr: 1.902 ± 0.326
2.282GlnVal: 2.282 ± 0.463
0.978GlnTrp: 0.978 ± 0.247
0.924GlnTyr: 0.924 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
7.607ArgAla: 7.607 ± 0.641
0.815ArgCys: 0.815 ± 0.218
5.053ArgAsp: 5.053 ± 0.549
4.455ArgGlu: 4.455 ± 0.49
1.576ArgPhe: 1.576 ± 0.286
5.107ArgGly: 5.107 ± 0.461
2.119ArgHis: 2.119 ± 0.417
2.934ArgIle: 2.934 ± 0.433
2.934ArgLys: 2.934 ± 0.418
5.977ArgLeu: 5.977 ± 0.608
2.445ArgMet: 2.445 ± 0.465
2.01ArgAsn: 2.01 ± 0.372
2.988ArgPro: 2.988 ± 0.38
2.771ArgGln: 2.771 ± 0.399
5.868ArgArg: 5.868 ± 0.83
3.64ArgSer: 3.64 ± 0.471
4.51ArgThr: 4.51 ± 0.417
4.021ArgVal: 4.021 ± 0.457
2.336ArgTrp: 2.336 ± 0.299
1.847ArgTyr: 1.847 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
5.596SerAla: 5.596 ± 0.485
0.869SerCys: 0.869 ± 0.196
4.075SerAsp: 4.075 ± 0.502
3.314SerGlu: 3.314 ± 0.391
1.25SerPhe: 1.25 ± 0.243
5.922SerGly: 5.922 ± 0.781
1.195SerHis: 1.195 ± 0.231
2.119SerIle: 2.119 ± 0.287
2.228SerLys: 2.228 ± 0.359
3.64SerLeu: 3.64 ± 0.473
1.902SerMet: 1.902 ± 0.343
1.25SerAsn: 1.25 ± 0.26
2.717SerPro: 2.717 ± 0.35
2.391SerGln: 2.391 ± 0.355
3.695SerArg: 3.695 ± 0.551
3.695SerSer: 3.695 ± 0.735
4.129SerThr: 4.129 ± 0.541
4.021SerVal: 4.021 ± 0.516
1.087SerTrp: 1.087 ± 0.21
1.576SerTyr: 1.576 ± 0.345
0.0SerXaa: 0.0 ± 0.0
Thr
7.607ThrAla: 7.607 ± 0.614
0.489ThrCys: 0.489 ± 0.185
5.053ThrAsp: 5.053 ± 0.656
3.477ThrGlu: 3.477 ± 0.448
1.793ThrPhe: 1.793 ± 0.311
4.727ThrGly: 4.727 ± 0.572
1.141ThrHis: 1.141 ± 0.264
3.314ThrIle: 3.314 ± 0.415
1.902ThrLys: 1.902 ± 0.364
5.162ThrLeu: 5.162 ± 0.569
1.087ThrMet: 1.087 ± 0.189
1.63ThrAsn: 1.63 ± 0.277
5.379ThrPro: 5.379 ± 0.571
2.391ThrGln: 2.391 ± 0.37
4.292ThrArg: 4.292 ± 0.525
3.749ThrSer: 3.749 ± 0.46
4.781ThrThr: 4.781 ± 0.681
5.433ThrVal: 5.433 ± 0.525
1.576ThrTrp: 1.576 ± 0.298
1.847ThrTyr: 1.847 ± 0.416
0.0ThrXaa: 0.0 ± 0.0
Val
9.019ValAla: 9.019 ± 0.837
1.087ValCys: 1.087 ± 0.255
5.107ValAsp: 5.107 ± 0.575
4.129ValGlu: 4.129 ± 0.54
1.793ValPhe: 1.793 ± 0.319
5.27ValGly: 5.27 ± 0.528
1.195ValHis: 1.195 ± 0.236
2.988ValIle: 2.988 ± 0.377
2.771ValLys: 2.771 ± 0.478
4.618ValLeu: 4.618 ± 0.485
1.358ValMet: 1.358 ± 0.284
2.499ValAsn: 2.499 ± 0.374
4.292ValPro: 4.292 ± 0.486
2.499ValGln: 2.499 ± 0.478
3.966ValArg: 3.966 ± 0.485
3.803ValSer: 3.803 ± 0.445
3.749ValThr: 3.749 ± 0.531
5.433ValVal: 5.433 ± 0.538
2.065ValTrp: 2.065 ± 0.401
1.467ValTyr: 1.467 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
1.739TrpAla: 1.739 ± 0.333
0.543TrpCys: 0.543 ± 0.173
1.576TrpAsp: 1.576 ± 0.223
0.815TrpGlu: 0.815 ± 0.21
0.598TrpPhe: 0.598 ± 0.169
1.576TrpGly: 1.576 ± 0.375
0.489TrpHis: 0.489 ± 0.16
1.793TrpIle: 1.793 ± 0.32
0.978TrpLys: 0.978 ± 0.204
2.173TrpLeu: 2.173 ± 0.363
0.652TrpMet: 0.652 ± 0.134
0.869TrpAsn: 0.869 ± 0.312
1.087TrpPro: 1.087 ± 0.257
1.467TrpGln: 1.467 ± 0.266
1.793TrpArg: 1.793 ± 0.335
1.195TrpSer: 1.195 ± 0.271
1.793TrpThr: 1.793 ± 0.287
1.141TrpVal: 1.141 ± 0.253
0.38TrpTrp: 0.38 ± 0.166
0.38TrpTyr: 0.38 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.576TyrAla: 1.576 ± 0.232
0.326TyrCys: 0.326 ± 0.132
2.01TyrAsp: 2.01 ± 0.343
1.902TyrGlu: 1.902 ± 0.326
0.978TyrPhe: 0.978 ± 0.224
2.228TyrGly: 2.228 ± 0.334
0.272TyrHis: 0.272 ± 0.125
0.869TyrIle: 0.869 ± 0.27
1.195TyrLys: 1.195 ± 0.278
1.847TyrLeu: 1.847 ± 0.328
0.435TyrMet: 0.435 ± 0.152
0.815TyrAsn: 0.815 ± 0.214
1.63TyrPro: 1.63 ± 0.313
0.706TyrGln: 0.706 ± 0.206
1.739TyrArg: 1.739 ± 0.298
1.63TyrSer: 1.63 ± 0.254
1.793TyrThr: 1.793 ± 0.406
1.25TyrVal: 1.25 ± 0.272
0.38TyrTrp: 0.38 ± 0.153
0.543TyrTyr: 0.543 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (18406 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski