Swingsia sp. F3b2
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1664 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y6U9P6|A0A4Y6U9P6_9PROT Glutathione S-transferase family protein OS=Swingsia sp. F3b2 OX=2558361 GN=E3E12_00075 PE=4 SV=1
MM1 pKa = 7.96 IPEE4 pKa = 4.41 DD5 pKa = 3.76 TYY7 pKa = 11.78 LDD9 pKa = 3.59 YY10 pKa = 11.6 DD11 pKa = 3.35 EE12 pKa = 5.22 WRR14 pKa = 11.84 FLSEE18 pKa = 3.95 FFYY21 pKa = 10.75 TSVHH25 pKa = 5.77 GEE27 pKa = 3.67 LRR29 pKa = 11.84 YY30 pKa = 7.19 TAKK33 pKa = 10.48 ILFHH37 pKa = 6.55 DD38 pKa = 3.79 VCIIEE43 pKa = 4.4 MTHH46 pKa = 6.49 EE47 pKa = 4.2 DD48 pKa = 4.78 AIDD51 pKa = 4.7 LIDD54 pKa = 3.85 VNFLSWSNKK63 pKa = 8.29 NGRR66 pKa = 11.84 IPLNYY71 pKa = 9.91 FMDD74 pKa = 3.44 VLEE77 pKa = 4.21 YY78 pKa = 10.83 SEE80 pKa = 5.31 EE81 pKa = 4.13 GLLEE85 pKa = 5.01 DD86 pKa = 5.34 DD87 pKa = 4.6 ADD89 pKa = 3.96 VYY91 pKa = 11.64 APDD94 pKa = 4.22 EE95 pKa = 4.28 YY96 pKa = 11.37 DD97 pKa = 3.43 EE98 pKa = 5.49 EE99 pKa = 4.42 EE100 pKa = 4.67 VEE102 pKa = 4.93 DD103 pKa = 4.49 EE104 pKa = 4.37 NPSSEE109 pKa = 4.36 SHH111 pKa = 6.23 SEE113 pKa = 4.08 APSSEE118 pKa = 4.04 ASSKK122 pKa = 10.93 DD123 pKa = 3.2
Molecular weight: 14.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.567
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.024
Thurlkill 3.605
EMBOSS 3.656
Sillero 3.884
Patrickios 0.731
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A4Y6UC03|A0A4Y6UC03_9PROT Uncharacterized protein OS=Swingsia sp. F3b2 OX=2558361 GN=E3E12_04945 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VINNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.68 GRR39 pKa = 11.84 KK40 pKa = 8.84 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1664
0
1664
551706
20
3659
331.6
35.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.63 ± 0.077
1.053 ± 0.023
4.728 ± 0.043
5.073 ± 0.055
3.198 ± 0.035
9.075 ± 0.083
2.662 ± 0.04
3.813 ± 0.045
3.454 ± 0.058
10.592 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.649 ± 0.031
2.842 ± 0.064
6.427 ± 0.061
4.287 ± 0.043
6.398 ± 0.063
5.23 ± 0.041
5.506 ± 0.059
6.89 ± 0.043
1.544 ± 0.03
1.95 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here