Desulfovibrio alaskensis (strain ATCC BAA 1058 / DSM 17464 / G20) (Desulfovibrio desulfuricans (strain G20))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; Desulfovibrio alaskensis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q30V48|Q30V48_DESAG Aldose 1-epimerase OS=Desulfovibrio alaskensis (strain ATCC BAA 1058 / DSM 17464 / G20) OX=207559 GN=Dde_3655 PE=3 SV=1
MM1 pKa = 7.51AKK3 pKa = 10.13VLIVYY8 pKa = 9.73GSTTGNTQTTAEE20 pKa = 4.15IVGTEE25 pKa = 4.32LKK27 pKa = 10.81NAGHH31 pKa = 7.09DD32 pKa = 3.44AVVNDD37 pKa = 3.44VTAVEE42 pKa = 4.61AEE44 pKa = 4.47GLCEE48 pKa = 4.97GYY50 pKa = 10.64DD51 pKa = 3.26AVLFGCSTWGDD62 pKa = 3.85EE63 pKa = 4.84EE64 pKa = 6.22IEE66 pKa = 4.17LQDD69 pKa = 5.49DD70 pKa = 5.07FIDD73 pKa = 6.3LYY75 pKa = 11.64DD76 pKa = 4.34EE77 pKa = 4.58LDD79 pKa = 3.39KK80 pKa = 11.74AGLSGVKK87 pKa = 9.75VGCFGCGDD95 pKa = 3.55SSYY98 pKa = 10.9QWFCGAVDD106 pKa = 5.07AIEE109 pKa = 4.28EE110 pKa = 4.11RR111 pKa = 11.84AKK113 pKa = 11.05EE114 pKa = 3.7NGARR118 pKa = 11.84IICDD122 pKa = 3.3SLKK125 pKa = 10.26IDD127 pKa = 4.71GDD129 pKa = 3.9PGSCRR134 pKa = 11.84SEE136 pKa = 3.76IADD139 pKa = 3.38WASAVGAALL148 pKa = 3.81

Molecular weight: 15.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q315Z7|Q315Z7_DESAG ABC-type transporter periplasmic subunit OS=Desulfovibrio alaskensis (strain ATCC BAA 1058 / DSM 17464 / G20) OX=207559 GN=Dde_0448 PE=4 SV=1
MM1 pKa = 8.02KK2 pKa = 8.89YY3 pKa = 7.73TCTITRR9 pKa = 11.84HH10 pKa = 4.93SSRR13 pKa = 11.84GTLRR17 pKa = 11.84GKK19 pKa = 8.55TVLRR23 pKa = 11.84AAPGEE28 pKa = 4.39VSWHH32 pKa = 5.87IALKK36 pKa = 9.84ILGYY40 pKa = 10.21ILFMPHH46 pKa = 5.55TPRR49 pKa = 11.84IEE51 pKa = 4.39EE52 pKa = 3.82NVGWHH57 pKa = 6.4FKK59 pKa = 10.34PDD61 pKa = 3.46LAAYY65 pKa = 9.64APDD68 pKa = 4.04GGVSLWVDD76 pKa = 3.91CGNIAVRR83 pKa = 11.84KK84 pKa = 8.18ISRR87 pKa = 11.84VAAWLPAAAEE97 pKa = 3.75FHH99 pKa = 6.0ILRR102 pKa = 11.84RR103 pKa = 11.84TVRR106 pKa = 11.84DD107 pKa = 3.29ASLLCASARR116 pKa = 11.84AVRR119 pKa = 11.84RR120 pKa = 11.84PEE122 pKa = 3.92RR123 pKa = 11.84VHH125 pKa = 6.1ITAFDD130 pKa = 3.36NGFVHH135 pKa = 6.74TLADD139 pKa = 3.65MLDD142 pKa = 3.25ATNRR146 pKa = 11.84MEE148 pKa = 5.22AVLDD152 pKa = 3.63NHH154 pKa = 6.75RR155 pKa = 11.84LALTVTCRR163 pKa = 11.84SGTATLHH170 pKa = 5.85SALHH174 pKa = 6.51RR175 pKa = 11.84LTPASGNPARR185 pKa = 11.84PRR187 pKa = 11.84SS188 pKa = 3.62

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3220

0

3220

1069418

31

3252

332.1

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.903 ± 0.061

1.452 ± 0.022

5.378 ± 0.033

6.132 ± 0.038

3.776 ± 0.028

8.033 ± 0.032

2.345 ± 0.023

4.812 ± 0.037

3.713 ± 0.037

10.349 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.022

2.805 ± 0.022

4.811 ± 0.029

3.619 ± 0.026

6.926 ± 0.038

5.49 ± 0.03

5.308 ± 0.029

7.489 ± 0.04

1.179 ± 0.018

2.588 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski