Amino acid dipepetide frequency for Rhodococcus jostii (strain RHA1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.176AlaAla: 18.176 ± 0.115	0.996AlaCys: 0.996 ± 0.017	7.983AlaAsp: 7.983 ± 0.057	7.596AlaGlu: 7.596 ± 0.061	3.498AlaPhe: 3.498 ± 0.043	11.269AlaGly: 11.269 ± 0.066	2.752AlaHis: 2.752 ± 0.03	4.756AlaIle: 4.756 ± 0.049	2.788AlaLys: 2.788 ± 0.042	12.81AlaLeu: 12.81 ± 0.094	2.532AlaMet: 2.532 ± 0.031	2.283AlaAsn: 2.283 ± 0.03	6.196AlaPro: 6.196 ± 0.055	3.631AlaGln: 3.631 ± 0.038	8.689AlaArg: 8.689 ± 0.067	6.305AlaSer: 6.305 ± 0.049	7.201AlaThr: 7.201 ± 0.057	11.515AlaVal: 11.515 ± 0.085	1.506AlaTrp: 1.506 ± 0.023	2.242AlaTyr: 2.242 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.076CysAla: 1.076 ± 0.021	0.135CysCys: 0.135 ± 0.008	0.502CysAsp: 0.502 ± 0.014	0.447CysGlu: 0.447 ± 0.012	0.23CysPhe: 0.23 ± 0.009	0.995CysGly: 0.995 ± 0.023	0.219CysHis: 0.219 ± 0.009	0.316CysIle: 0.316 ± 0.009	0.122CysLys: 0.122 ± 0.007	0.69CysLeu: 0.69 ± 0.016	0.141CysMet: 0.141 ± 0.007	0.168CysAsn: 0.168 ± 0.008	0.561CysPro: 0.561 ± 0.015	0.197CysGln: 0.197 ± 0.009	0.756CysArg: 0.756 ± 0.016	0.599CysSer: 0.599 ± 0.015	0.582CysThr: 0.582 ± 0.014	0.657CysVal: 0.657 ± 0.015	0.11CysTrp: 0.11 ± 0.006	0.168CysTyr: 0.168 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.69AspAla: 7.69 ± 0.046	0.445AspCys: 0.445 ± 0.014	3.883AspAsp: 3.883 ± 0.043	3.982AspGlu: 3.982 ± 0.037	1.704AspPhe: 1.704 ± 0.028	5.779AspGly: 5.779 ± 0.052	1.527AspHis: 1.527 ± 0.025	2.406AspIle: 2.406 ± 0.032	1.159AspLys: 1.159 ± 0.024	6.484AspLeu: 6.484 ± 0.054	0.881AspMet: 0.881 ± 0.02	1.167AspAsn: 1.167 ± 0.021	4.361AspPro: 4.361 ± 0.048	1.663AspGln: 1.663 ± 0.025	5.091AspArg: 5.091 ± 0.05	3.153AspSer: 3.153 ± 0.032	3.606AspThr: 3.606 ± 0.032	5.479AspVal: 5.479 ± 0.062	0.919AspTrp: 0.919 ± 0.02	1.283AspTyr: 1.283 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	5.809GluAla: 5.809 ± 0.049	0.416GluCys: 0.416 ± 0.012	2.744GluAsp: 2.744 ± 0.032	2.814GluGlu: 2.814 ± 0.04	1.844GluPhe: 1.844 ± 0.028	3.577GluGly: 3.577 ± 0.038	1.67GluHis: 1.67 ± 0.027	2.709GluIle: 2.709 ± 0.032	1.496GluLys: 1.496 ± 0.028	6.427GluLeu: 6.427 ± 0.061	1.032GluMet: 1.032 ± 0.019	1.352GluAsn: 1.352 ± 0.022	3.058GluPro: 3.058 ± 0.041	2.323GluGln: 2.323 ± 0.031	4.785GluArg: 4.785 ± 0.053	3.191GluSer: 3.191 ± 0.038	2.979GluThr: 2.979 ± 0.032	4.364GluVal: 4.364 ± 0.044	0.873GluTrp: 0.873 ± 0.018	1.177GluTyr: 1.177 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.015PheAla: 4.015 ± 0.039	0.296PheCys: 0.296 ± 0.01	2.489PheAsp: 2.489 ± 0.042	1.692PheGlu: 1.692 ± 0.027	1.03PhePhe: 1.03 ± 0.023	3.414PheGly: 3.414 ± 0.039	0.702PheHis: 0.702 ± 0.017	0.969PheIle: 0.969 ± 0.02	0.432PheLys: 0.432 ± 0.014	2.584PheLeu: 2.584 ± 0.038	0.459PheMet: 0.459 ± 0.014	0.569PheAsn: 0.569 ± 0.014	1.45PhePro: 1.45 ± 0.021	0.682PheGln: 0.682 ± 0.019	1.847PheArg: 1.847 ± 0.027	1.676PheSer: 1.676 ± 0.025	2.133PheThr: 2.133 ± 0.032	2.747PheVal: 2.747 ± 0.035	0.413PheTrp: 0.413 ± 0.013	0.645PheTyr: 0.645 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	9.921GlyAla: 9.921 ± 0.075	0.86GlyCys: 0.86 ± 0.021	5.029GlyAsp: 5.029 ± 0.042	4.621GlyGlu: 4.621 ± 0.047	3.139GlyPhe: 3.139 ± 0.041	7.85GlyGly: 7.85 ± 0.08	2.11GlyHis: 2.11 ± 0.032	4.354GlyIle: 4.354 ± 0.043	2.322GlyLys: 2.322 ± 0.032	8.394GlyLeu: 8.394 ± 0.068	1.982GlyMet: 1.982 ± 0.028	1.929GlyAsn: 1.929 ± 0.032	4.463GlyPro: 4.463 ± 0.043	2.536GlyGln: 2.536 ± 0.036	6.761GlyArg: 6.761 ± 0.06	5.549GlySer: 5.549 ± 0.049	5.773GlyThr: 5.773 ± 0.053	7.569GlyVal: 7.569 ± 0.056	1.578GlyTrp: 1.578 ± 0.025	2.289GlyTyr: 2.289 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.559HisAla: 2.559 ± 0.028	0.251HisCys: 0.251 ± 0.01	1.437HisAsp: 1.437 ± 0.024	1.17HisGlu: 1.17 ± 0.023	0.721HisPhe: 0.721 ± 0.014	2.172HisGly: 2.172 ± 0.031	0.832HisHis: 0.832 ± 0.027	0.855HisIle: 0.855 ± 0.02	0.332HisLys: 0.332 ± 0.012	2.339HisLeu: 2.339 ± 0.029	0.39HisMet: 0.39 ± 0.012	0.454HisAsn: 0.454 ± 0.012	1.814HisPro: 1.814 ± 0.028	0.67HisGln: 0.67 ± 0.017	2.226HisArg: 2.226 ± 0.033	1.237HisSer: 1.237 ± 0.022	1.372HisThr: 1.372 ± 0.029	1.775HisVal: 1.775 ± 0.028	0.355HisTrp: 0.355 ± 0.01	0.515HisTyr: 0.515 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	6.127IleAla: 6.127 ± 0.055	0.402IleCys: 0.402 ± 0.014	3.274IleAsp: 3.274 ± 0.031	2.59IleGlu: 2.59 ± 0.034	1.06IlePhe: 1.06 ± 0.023	4.519IleGly: 4.519 ± 0.045	0.807IleHis: 0.807 ± 0.016	1.322IleIle: 1.322 ± 0.024	0.739IleLys: 0.739 ± 0.018	3.423IleLeu: 3.423 ± 0.042	0.594IleMet: 0.594 ± 0.014	0.916IleAsn: 0.916 ± 0.02	2.4IlePro: 2.4 ± 0.027	0.854IleGln: 0.854 ± 0.017	2.73IleArg: 2.73 ± 0.031	2.352IleSer: 2.352 ± 0.029	2.728IleThr: 2.728 ± 0.032	3.799IleVal: 3.799 ± 0.037	0.436IleTrp: 0.436 ± 0.011	0.74IleTyr: 0.74 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.541LysAla: 2.541 ± 0.043	0.135LysCys: 0.135 ± 0.007	1.066LysAsp: 1.066 ± 0.023	0.996LysGlu: 0.996 ± 0.023	0.63LysPhe: 0.63 ± 0.015	1.575LysGly: 1.575 ± 0.028	0.484LysHis: 0.484 ± 0.013	1.082LysIle: 1.082 ± 0.023	0.742LysLys: 0.742 ± 0.026	2.044LysLeu: 2.044 ± 0.027	0.46LysMet: 0.46 ± 0.013	0.522LysAsn: 0.522 ± 0.013	1.241LysPro: 1.241 ± 0.025	0.682LysGln: 0.682 ± 0.015	1.419LysArg: 1.419 ± 0.023	1.274LysSer: 1.274 ± 0.023	1.313LysThr: 1.313 ± 0.028	1.834LysVal: 1.834 ± 0.029	0.302LysTrp: 0.302 ± 0.012	0.487LysTyr: 0.487 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	13.279LeuAla: 13.279 ± 0.092	0.843LeuCys: 0.843 ± 0.019	7.059LeuAsp: 7.059 ± 0.071	4.793LeuGlu: 4.793 ± 0.047	2.801LeuPhe: 2.801 ± 0.038	9.062LeuGly: 9.062 ± 0.083	2.143LeuHis: 2.143 ± 0.03	3.881LeuIle: 3.881 ± 0.045	1.552LeuLys: 1.552 ± 0.026	9.403LeuLeu: 9.403 ± 0.074	1.656LeuMet: 1.656 ± 0.026	1.807LeuAsn: 1.807 ± 0.028	5.745LeuPro: 5.745 ± 0.055	2.385LeuGln: 2.385 ± 0.033	7.508LeuArg: 7.508 ± 0.06	5.834LeuSer: 5.834 ± 0.048	6.552LeuThr: 6.552 ± 0.057	8.718LeuVal: 8.718 ± 0.063	1.17LeuTrp: 1.17 ± 0.017	1.6LeuTyr: 1.6 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.191MetAla: 2.191 ± 0.028	0.196MetCys: 0.196 ± 0.009	0.834MetAsp: 0.834 ± 0.02	0.683MetGlu: 0.683 ± 0.016	0.62MetPhe: 0.62 ± 0.015	1.363MetGly: 1.363 ± 0.025	0.408MetHis: 0.408 ± 0.012	0.91MetIle: 0.91 ± 0.02	0.484MetLys: 0.484 ± 0.014	1.914MetLeu: 1.914 ± 0.027	0.393MetMet: 0.393 ± 0.011	0.521MetAsn: 0.521 ± 0.014	1.103MetPro: 1.103 ± 0.02	0.549MetGln: 0.549 ± 0.013	1.434MetArg: 1.434 ± 0.023	1.644MetSer: 1.644 ± 0.024	1.815MetThr: 1.815 ± 0.025	1.529MetVal: 1.529 ± 0.025	0.245MetTrp: 0.245 ± 0.011	0.349MetTyr: 0.349 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.438AsnAla: 2.438 ± 0.031	0.189AsnCys: 0.189 ± 0.009	1.092AsnAsp: 1.092 ± 0.02	0.944AsnGlu: 0.944 ± 0.018	0.636AsnPhe: 0.636 ± 0.016	1.933AsnGly: 1.933 ± 0.027	0.443AsnHis: 0.443 ± 0.014	0.877AsnIle: 0.877 ± 0.017	0.442AsnLys: 0.442 ± 0.014	2.031AsnLeu: 2.031 ± 0.029	0.387AsnMet: 0.387 ± 0.013	0.494AsnAsn: 0.494 ± 0.015	1.62AsnPro: 1.62 ± 0.028	0.587AsnGln: 0.587 ± 0.016	1.53AsnArg: 1.53 ± 0.026	1.216AsnSer: 1.216 ± 0.025	1.3AsnThr: 1.3 ± 0.026	1.689AsnVal: 1.689 ± 0.026	0.342AsnTrp: 0.342 ± 0.01	0.533AsnTyr: 0.533 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	7.17ProAla: 7.17 ± 0.071	0.359ProCys: 0.359 ± 0.011	4.41ProAsp: 4.41 ± 0.044	3.863ProGlu: 3.863 ± 0.04	1.634ProPhe: 1.634 ± 0.026	5.562ProGly: 5.562 ± 0.047	1.37ProHis: 1.37 ± 0.024	2.185ProIle: 2.185 ± 0.029	1.154ProLys: 1.154 ± 0.022	4.902ProLeu: 4.902 ± 0.051	1.139ProMet: 1.139 ± 0.021	1.16ProAsn: 1.16 ± 0.023	3.183ProPro: 3.183 ± 0.051	1.652ProGln: 1.652 ± 0.028	3.763ProArg: 3.763 ± 0.053	3.389ProSer: 3.389 ± 0.037	3.695ProThr: 3.695 ± 0.041	5.214ProVal: 5.214 ± 0.053	0.785ProTrp: 0.785 ± 0.017	1.071ProTyr: 1.071 ± 0.016	0.0ProXaa: 0.0 ± 0.0
Gln	3.181GlnAla: 3.181 ± 0.037	0.216GlnCys: 0.216 ± 0.008	1.266GlnAsp: 1.266 ± 0.021	1.199GlnGlu: 1.199 ± 0.021	0.937GlnPhe: 0.937 ± 0.018	1.948GlnGly: 1.948 ± 0.028	0.732GlnHis: 0.732 ± 0.016	1.511GlnIle: 1.511 ± 0.023	0.619GlnLys: 0.619 ± 0.017	3.279GlnLeu: 3.279 ± 0.04	0.602GlnMet: 0.602 ± 0.017	0.624GlnAsn: 0.624 ± 0.014	1.605GlnPro: 1.605 ± 0.023	1.257GlnGln: 1.257 ± 0.022	2.417GlnArg: 2.417 ± 0.034	1.614GlnSer: 1.614 ± 0.022	1.464GlnThr: 1.464 ± 0.024	2.494GlnVal: 2.494 ± 0.036	0.531GlnTrp: 0.531 ± 0.014	0.625GlnTyr: 0.625 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	8.276ArgAla: 8.276 ± 0.057	0.759ArgCys: 0.759 ± 0.02	4.333ArgAsp: 4.333 ± 0.039	4.247ArgGlu: 4.247 ± 0.046	2.377ArgPhe: 2.377 ± 0.029	5.577ArgGly: 5.577 ± 0.057	1.966ArgHis: 1.966 ± 0.032	3.692ArgIle: 3.692 ± 0.037	1.741ArgLys: 1.741 ± 0.032	7.196ArgLeu: 7.196 ± 0.063	1.804ArgMet: 1.804 ± 0.026	1.66ArgAsn: 1.66 ± 0.027	4.262ArgPro: 4.262 ± 0.043	2.224ArgGln: 2.224 ± 0.031	7.093ArgArg: 7.093 ± 0.075	4.749ArgSer: 4.749 ± 0.046	4.993ArgThr: 4.993 ± 0.042	5.748ArgVal: 5.748 ± 0.043	1.327ArgTrp: 1.327 ± 0.02	1.761ArgTyr: 1.761 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	7.218SerAla: 7.218 ± 0.056	0.506SerCys: 0.506 ± 0.017	3.401SerAsp: 3.401 ± 0.033	2.987SerGlu: 2.987 ± 0.036	1.724SerPhe: 1.724 ± 0.027	6.018SerGly: 6.018 ± 0.054	1.169SerHis: 1.169 ± 0.021	2.381SerIle: 2.381 ± 0.031	1.206SerLys: 1.206 ± 0.025	5.153SerLeu: 5.153 ± 0.042	1.381SerMet: 1.381 ± 0.021	1.163SerAsn: 1.163 ± 0.021	3.483SerPro: 3.483 ± 0.044	1.458SerGln: 1.458 ± 0.025	4.399SerArg: 4.399 ± 0.04	3.617SerSer: 3.617 ± 0.048	3.934SerThr: 3.934 ± 0.033	4.97SerVal: 4.97 ± 0.045	0.928SerTrp: 0.928 ± 0.019	1.174SerTyr: 1.174 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	8.182ThrAla: 8.182 ± 0.065	0.51ThrCys: 0.51 ± 0.015	3.939ThrAsp: 3.939 ± 0.039	3.494ThrGlu: 3.494 ± 0.039	1.838ThrPhe: 1.838 ± 0.027	6.163ThrGly: 6.163 ± 0.048	1.386ThrHis: 1.386 ± 0.033	2.545ThrIle: 2.545 ± 0.032	1.209ThrLys: 1.209 ± 0.022	5.863ThrLeu: 5.863 ± 0.05	1.16ThrMet: 1.16 ± 0.017	1.219ThrAsn: 1.219 ± 0.025	4.196ThrPro: 4.196 ± 0.041	1.5ThrGln: 1.5 ± 0.024	4.149ThrArg: 4.149 ± 0.038	3.731ThrSer: 3.731 ± 0.036	4.43ThrThr: 4.43 ± 0.058	6.265ThrVal: 6.265 ± 0.048	0.841ThrTrp: 0.841 ± 0.019	1.298ThrTyr: 1.298 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	11.119ValAla: 11.119 ± 0.075	0.782ValCys: 0.782 ± 0.017	5.905ValAsp: 5.905 ± 0.047	4.836ValGlu: 4.836 ± 0.049	2.589ValPhe: 2.589 ± 0.038	7.269ValGly: 7.269 ± 0.058	1.976ValHis: 1.976 ± 0.029	3.7ValIle: 3.7 ± 0.04	1.577ValLys: 1.577 ± 0.028	9.094ValLeu: 9.094 ± 0.066	1.538ValMet: 1.538 ± 0.024	1.875ValAsn: 1.875 ± 0.026	5.041ValPro: 5.041 ± 0.044	2.136ValGln: 2.136 ± 0.028	6.226ValArg: 6.226 ± 0.055	4.93ValSer: 4.93 ± 0.041	5.916ValThr: 5.916 ± 0.06	8.769ValVal: 8.769 ± 0.077	1.082ValTrp: 1.082 ± 0.021	1.486ValTyr: 1.486 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.521TrpAla: 1.521 ± 0.025	0.168TrpCys: 0.168 ± 0.008	0.734TrpAsp: 0.734 ± 0.019	0.649TrpGlu: 0.649 ± 0.015	0.51TrpPhe: 0.51 ± 0.015	1.008TrpGly: 1.008 ± 0.021	0.344TrpHis: 0.344 ± 0.01	0.704TrpIle: 0.704 ± 0.016	0.369TrpLys: 0.369 ± 0.013	1.533TrpLeu: 1.533 ± 0.027	0.35TrpMet: 0.35 ± 0.01	0.426TrpAsn: 0.426 ± 0.012	0.712TrpPro: 0.712 ± 0.017	0.534TrpGln: 0.534 ± 0.014	1.202TrpArg: 1.202 ± 0.024	1.015TrpSer: 1.015 ± 0.02	0.945TrpThr: 0.945 ± 0.017	1.091TrpVal: 1.091 ± 0.022	0.3TrpTrp: 0.3 ± 0.011	0.303TrpTyr: 0.303 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.237TyrAla: 2.237 ± 0.032	0.207TyrCys: 0.207 ± 0.008	1.284TyrAsp: 1.284 ± 0.024	1.034TyrGlu: 1.034 ± 0.022	0.722TyrPhe: 0.722 ± 0.015	1.979TyrGly: 1.979 ± 0.029	0.437TyrHis: 0.437 ± 0.013	0.657TyrIle: 0.657 ± 0.017	0.342TyrLys: 0.342 ± 0.012	2.23TyrLeu: 2.23 ± 0.032	0.303TyrMet: 0.303 ± 0.012	0.413TyrAsn: 0.413 ± 0.012	1.164TyrPro: 1.164 ± 0.022	0.601TyrGln: 0.601 ± 0.017	1.763TyrArg: 1.763 ± 0.026	1.195TyrSer: 1.195 ± 0.021	1.186TyrThr: 1.186 ± 0.023	1.62TyrVal: 1.62 ± 0.029	0.372TyrTrp: 0.372 ± 0.012	0.447TyrTyr: 0.447 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9075 proteins (2871138 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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