Devosia sp. YR412
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3685 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H8ZY17|A0A1H8ZY17_9RHIZ Iron complex transport system ATP-binding protein OS=Devosia sp. YR412 OX=1881030 GN=SAMN05428969_0494 PE=3 SV=1
MM1 pKa = 7.94 LDD3 pKa = 3.53 GLTLGEE9 pKa = 3.55 IHH11 pKa = 6.33 MRR13 pKa = 11.84 LVLVIALLLSTAPAFAADD31 pKa = 3.38 AVIYY35 pKa = 10.56 KK36 pKa = 8.49 GTLGGKK42 pKa = 9.97 DD43 pKa = 2.86 IVVEE47 pKa = 4.12 LTDD50 pKa = 3.92 PSADD54 pKa = 3.27 QVVGRR59 pKa = 11.84 YY60 pKa = 9.31 SYY62 pKa = 11.2 LSQGGDD68 pKa = 3.08 IPLSSVDD75 pKa = 3.28 TSEE78 pKa = 4.83 GVVTLAEE85 pKa = 4.15 EE86 pKa = 4.71 APCTEE91 pKa = 4.37 TTCVFDD97 pKa = 4.65 DD98 pKa = 3.76 NYY100 pKa = 10.8 EE101 pKa = 4.06 IVEE104 pKa = 4.33 VPIGAHH110 pKa = 3.88 WQLRR114 pKa = 11.84 VKK116 pKa = 10.72 DD117 pKa = 4.44 DD118 pKa = 3.71 GSITGGWNPADD129 pKa = 3.58 GSAVLDD135 pKa = 3.58 IEE137 pKa = 4.29 LTEE140 pKa = 4.12 VARR143 pKa = 11.84 RR144 pKa = 11.84 TLPEE148 pKa = 4.54 DD149 pKa = 3.5 MAITPADD156 pKa = 3.84 LADD159 pKa = 4.1 SAWVASYY166 pKa = 11.08 DD167 pKa = 3.57 NPAAFSRR174 pKa = 11.84 DD175 pKa = 3.13 SAPYY179 pKa = 10.36 DD180 pKa = 3.53 FAKK183 pKa = 10.09 MDD185 pKa = 3.46 VALTEE190 pKa = 4.58 GPTRR194 pKa = 11.84 TMDD197 pKa = 3.34 GNSYY201 pKa = 10.76 RR202 pKa = 11.84 DD203 pKa = 3.68 VIDD206 pKa = 4.23 PRR208 pKa = 11.84 SKK210 pKa = 10.23 FPFPRR215 pKa = 11.84 VVAFADD221 pKa = 4.57 GSPVEE226 pKa = 4.18 AANTRR231 pKa = 11.84 LAARR235 pKa = 11.84 HH236 pKa = 5.26 AQINMSAFACLSKK249 pKa = 10.76 AYY251 pKa = 10.18 AGLGYY256 pKa = 9.7 RR257 pKa = 11.84 DD258 pKa = 3.46 GMGGGGLGDD267 pKa = 4.04 FDD269 pKa = 4.76 GEE271 pKa = 4.59 SIEE274 pKa = 4.33 IAYY277 pKa = 9.82 LSPTVINWTEE287 pKa = 3.93 TGSTYY292 pKa = 10.73 CGGAYY297 pKa = 9.43 PNNHH301 pKa = 6.43 FDD303 pKa = 4.53 SYY305 pKa = 11.1 IIDD308 pKa = 3.72 ARR310 pKa = 11.84 TGADD314 pKa = 3.84 FALGWVLKK322 pKa = 10.48 DD323 pKa = 3.85 WIATADD329 pKa = 3.52 TTNYY333 pKa = 10.52 EE334 pKa = 4.15 PVTDD338 pKa = 3.69 QAAALEE344 pKa = 4.38 NPNGYY349 pKa = 9.69 LFAPGQPLIDD359 pKa = 3.7 YY360 pKa = 10.15 ALAYY364 pKa = 10.34 LDD366 pKa = 4.43 EE367 pKa = 6.24 AGLDD371 pKa = 3.4 PDD373 pKa = 5.18 LASEE377 pKa = 4.39 CQLPDD382 pKa = 4.89 LIAEE386 pKa = 4.51 GNLGFRR392 pKa = 11.84 FAPEE396 pKa = 3.79 NKK398 pKa = 9.76 LVFAITGLAHH408 pKa = 7.27 ANFACSEE415 pKa = 4.17 DD416 pKa = 3.83 VLTVSLSDD424 pKa = 3.49 IPEE427 pKa = 4.35 LLALTASDD435 pKa = 4.48 YY436 pKa = 11.27 FPDD439 pKa = 4.14 LANN442 pKa = 3.68
Molecular weight: 47.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.694
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.605
Solomon 3.91
Lehninger 3.859
Nozaki 4.012
DTASelect 4.266
Thurlkill 3.745
EMBOSS 3.846
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.927
Protein with the highest isoelectric point:
>tr|A0A1H9CDL2|A0A1H9CDL2_9RHIZ Transcriptional regulator HxlR family OS=Devosia sp. YR412 OX=1881030 GN=SAMN05428969_1445 PE=4 SV=1
MM1 pKa = 7.67 PALILGLAVLLVVTMSVAVGAGAVPIPVDD30 pKa = 3.75 TVWRR34 pKa = 11.84 IIASKK39 pKa = 9.89 LAPGSVVADD48 pKa = 3.43 WTSGRR53 pKa = 11.84 EE54 pKa = 3.93 NIVWEE59 pKa = 4.15 VRR61 pKa = 11.84 LPRR64 pKa = 11.84 IVLGAIVGASLAVVGAALQSVTRR87 pKa = 11.84 NPLADD92 pKa = 3.56 PHH94 pKa = 7.59 LLGISSGAAFGAILVLLHH112 pKa = 6.37 TGMFLGLITVPLFAFIGALIATALVLAVSNLTKK145 pKa = 10.48 SQSAGRR151 pKa = 11.84 LVLAGVAVSFIITAAGNLFIFLGDD175 pKa = 3.23 PRR177 pKa = 11.84 AAHH180 pKa = 5.55 TVVFWMLGGLGLAQWQQLPFPLAALVLCGGYY211 pKa = 10.48 LILKK215 pKa = 9.56 ARR217 pKa = 11.84 SLNAMTLGDD226 pKa = 4.11 EE227 pKa = 4.45 SATTLGIPALRR238 pKa = 11.84 FRR240 pKa = 11.84 LTVFVICALLTGSAVAFSGVIAFVGLMIPHH270 pKa = 7.6 IVRR273 pKa = 11.84 MMVGGDD279 pKa = 3.5 YY280 pKa = 10.81 RR281 pKa = 11.84 RR282 pKa = 11.84 VLPLSALVGAIFLVLADD299 pKa = 3.81 IAARR303 pKa = 11.84 TLMPPQDD310 pKa = 3.76 MPIGVLTGIIGGLFFVGLMRR330 pKa = 11.84 WRR332 pKa = 11.84 KK333 pKa = 9.87 SGEE336 pKa = 3.7
Molecular weight: 34.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.487
IPC_protein 10.452
Toseland 10.657
ProMoST 10.452
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.804
Grimsley 10.789
Solomon 10.921
Lehninger 10.877
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.082
Sillero 10.687
Patrickios 10.57
IPC_peptide 10.921
IPC2_peptide 9.721
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3685
0
3685
1143512
29
3895
310.3
33.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.346 ± 0.058
0.627 ± 0.01
5.772 ± 0.032
5.517 ± 0.034
3.805 ± 0.027
8.47 ± 0.039
1.952 ± 0.019
5.52 ± 0.026
3.288 ± 0.034
10.323 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.022
2.862 ± 0.027
4.942 ± 0.029
3.327 ± 0.026
6.122 ± 0.038
5.566 ± 0.03
5.708 ± 0.045
7.695 ± 0.033
1.303 ± 0.019
2.286 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here