Rhodobacter phage RcTitan

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Titanvirus; Rhodobacter virus RcTitan

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1LLJ1|A0A0K1LLJ1_9CAUD Portal protein OS=Rhodobacter phage RcTitan OX=1662330 GN=RCTITAN_8 PE=4 SV=1
MM1 pKa = 7.16TGEE4 pKa = 3.75TAFWLFVLWGCGVFFLICFMMGANHH29 pKa = 7.66DD30 pKa = 4.09DD31 pKa = 3.84DD32 pKa = 4.44

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1LKW6|A0A0K1LKW6_9CAUD VRR endonuclease OS=Rhodobacter phage RcTitan OX=1662330 GN=RCTITAN_53 PE=4 SV=1
MM1 pKa = 7.36TPPRR5 pKa = 11.84SKK7 pKa = 10.53PCPACAAPCGVAATACRR24 pKa = 11.84VCGHH28 pKa = 5.36VMPRR32 pKa = 11.84KK33 pKa = 9.95GGGRR37 pKa = 11.84PAPTPEE43 pKa = 3.52QRR45 pKa = 11.84LAVALTRR52 pKa = 11.84QAVADD57 pKa = 3.66GHH59 pKa = 6.44KK60 pKa = 9.6RR61 pKa = 11.84GPMPEE66 pKa = 3.68PLGRR70 pKa = 11.84RR71 pKa = 11.84NLSKK75 pKa = 10.81RR76 pKa = 11.84GAKK79 pKa = 9.47VLALIRR85 pKa = 11.84QFASNGRR92 pKa = 11.84NATEE96 pKa = 3.31IAAAIGCSVTNVRR109 pKa = 11.84RR110 pKa = 11.84YY111 pKa = 8.9AHH113 pKa = 7.42DD114 pKa = 3.51YY115 pKa = 9.9GVRR118 pKa = 11.84IVKK121 pKa = 10.45GEE123 pKa = 4.14SGPVMNGKK131 pKa = 10.14LYY133 pKa = 9.5AQTVEE138 pKa = 4.15RR139 pKa = 11.84VEE141 pKa = 4.3GCALIMQRR149 pKa = 11.84TTVVAEE155 pKa = 3.89VAEE158 pKa = 4.04ALGVCKK164 pKa = 9.86ATARR168 pKa = 11.84EE169 pKa = 3.85YY170 pKa = 9.7MKK172 pKa = 10.32RR173 pKa = 11.84VRR175 pKa = 11.84DD176 pKa = 3.62VTEE179 pKa = 4.04IEE181 pKa = 4.82LPGFDD186 pKa = 3.37QRR188 pKa = 11.84KK189 pKa = 8.68ASGFAVTRR197 pKa = 11.84RR198 pKa = 11.84LGRR201 pKa = 11.84KK202 pKa = 6.21PTLGKK207 pKa = 9.01PAKK210 pKa = 10.14

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14280

32

1052

234.1

25.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.549 ± 0.791

0.959 ± 0.125

5.42 ± 0.286

6.246 ± 0.338

4.335 ± 0.216

8.158 ± 0.354

1.12 ± 0.115

4.951 ± 0.223

5.469 ± 0.38

7.437 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.132

4.398 ± 0.183

5.042 ± 0.343

3.761 ± 0.399

5.315 ± 0.306

5.504 ± 0.236

6.113 ± 0.329

7.024 ± 0.254

1.218 ± 0.137

2.675 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski